• Journal of Pharmaceutical Investigation
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• 제40권4호
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• pp.201-212
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• 2010

Self-organized nanogels from polysaccharide derivatives offer a promising approach in treatment of cancer due to their flexibility in chemistry and their ability to improve the therapeutic index of a drug by modifying biodistribution by their preferential localization at target sites and lower distribution in normal healthy tissues. These properties have promoted studies of active cancer targeting by self-organized nanogels for even better accumulation in solid tumors. However although many researchers have reported their potential by using cell culture systems and small animal tumor models in cancer therapy, these nanogels need more decoration such as conjugation with targeting moiety and endowment of stimuli-sensitivity for precise targeting of the cancer site. In this review, we summarize the recent efforts in developing novel targeting approaches via active endocytosis and stimuli-sensitive systems responding to hyperthermic or acidic tumor pH conditions.

• Molecules and Cells
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• 제46권1호
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• pp.21-32
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• 2023

MicroRNAs (miRNAs) play cardinal roles in regulating biological pathways and processes, resulting in significant physiological effects. To understand the complex regulatory network of miRNAs, previous studies have utilized massivescale datasets of miRNA targeting and attempted to computationally predict the functional targets of miRNAs. Many miRNA target prediction tools have been developed and are widely used by scientists from various fields of biology and medicine. Most of these tools consider seed pairing between miRNAs and their mRNA targets and additionally consider other determinants to improve prediction accuracy. However, these tools exhibit limited prediction accuracy and high false positive rates. The utilization of additional determinants, such as RNA modifications and RNA-binding protein binding sites, may further improve miRNA target prediction. In this review, we discuss the determinants of functional miRNA targeting that are currently used in miRNA target prediction and the potentially predictive but unappreciated determinants that may improve prediction accuracy.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제16권12호
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• pp.1154-1164
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• 2010

타겟팅 서비스는 다양한 미디어 서비스 환경에서 사용자 프로파일, 선호도 및 사용 내역 등을 기반으로 사용자의 취향에 가장 적합한 서비스를 추천 및 제공함으로써, 서비스의 만족도와 이용량을 향상 시키는 주요한 응용서비스로서 주로 방송분야에서 연구되어 왔다. 타겟팅 서비스는 방송 콘렌츠에서 interstitial 콘텐츠로, 고정형 TV단말에서 모바일 단말로 서비스의 영역이 확대되고 있으며, 단순한 방송 데이터에서 광고 데이터, 쿠폰 및 관련 미디어 콘텐츠 정보 등으로 데이터의 종류가 다양해지고 있다. 본 논문에서는 사용자 정보를 기반으로 기사, 광고 및 방송정보에 대한 타겟팅 데이터 서비스를 설계, 구현하고자 한다. 이를 위해 웹 기반의 미디어 콘텐츠에 적용할 수 있도록 사용자 프로파일, 선호도 및 사용 내역 정보를 기존 TV-Anytime Forum의 사용자 메타데이터 및 OpenSocial의 사용자 정보를 기반으로 새롭게 정의하였다. 또한, 사용자 정보와 콘텐츠 정보간 유사도 및 사용 내역을 기반으로 사용자 선호도 정보 및 행동 패턴 정보를 생성하여 타겟팅 데이터 서비스를 구현하였다. 성능평가를 통해서 제안하는 타겟팅 데이터 서비스 기술이 기존의 방송 서비스뿐만 아니라, 웹 기반의 미디어 콘텐츠에서도 적용 가능 하다는 것을 확인하였다.

• Archives of Pharmacal Research
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• 제29권12호
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• pp.1164-1170
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• 2006

A triptolide-lysozyme (TP-LZM) conjugate was synthesized to achieve renal specific delivery and to reduce the side effects of triptolide. Triptolide was coupled to lysozyme through succinic via an ester bond with an average coupling degree of 1 mol triptolide per 1 mol lysozyme. The lysozyme can specifically accumulate in the proximal tubular cells of the kidney, making it a potential carrier for targeting drugs to the kidney. The structure of triptolide succinate (TPS) was confirmed by IR, $^{1}H-NMR$, MS and UV. The concentrations of triptolide in various samples were determined by reversed-phase high-performance liquid chromatography (HPLC). In this study, the physicochemical and stability profiles of TP-LZM under various conditions were investgated the stability and releasing profiles of triptolide-lysozyme (TP-LZM) under various conditions. In vitro release trails showed triptolide-lysozyme was relatively stable in plasma (less than 30% of free triptolide released) and could release triptolide quickly in lysosome (more than 80% of free triptolide released) at $37^{\circ}C$ for 24 h. In addition, the biological activities of the conjugate on normal rat kidney proximal tubular cells (NRK52E) were also tested. The conjugate can effectively reduce NO production in the medium of NRK52E induced by lipopolysaccharide (LPS) but with much lower toxicity. These studies suggest the possibility to promote curative effect and reduce its extra-renal toxicity of triptolide by TP-LZM conjugate.

• Child Health Nursing Research
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• 제8권3호
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• pp.334-343
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• 2002

This study was aimed at primarily to develop a tool to evaluate sexuality education and secondarily to test effectiveness through application of developed evaluation tools in elementary school. The results from this study were summarized below: 1. On the basis of targeting the lower grades (1st- 3th year) and the higher grades (4th-6th year) elementary school students' sexuality education guidebooks published by Korea Ministry of Education & Human Resources Development, 71 preliminary items targeting the lower grades, 90 preliminary items targeting the higher grades were developed. 2. Through the validity test about the contents of the preliminary items three times, the items were regulated to 65 items targeting the lower grads and 57 items targeting the higher grades. And then, the preliminary items were re-regulated to 40 items separately. Then, pre-test which targeting each 30 students was enforced. 3.Finally, the evaluation tools for sexuality education that consisted of 40 items targeting the lower grades and the higher grades were developed. 4. eliability test of the developed tools, sexuality education evaluation tools showed alpha coefficient of internal consistency were 0.8355 (for the use of the lower grades) and 0.8881 (for the use of the higher grades). 5. o apply the developed sexuality education evaluation tools, 10-times sexuality education were carried out class unit and pre-post test were done using same questionnaire, which contains developed tool, there were significant difference in low grade (t=16.548, p=0.000), high grade (t=14.773, p=0.000). The results of this study suggest that the evaluation tool for sexuality education in elementary school may be a useful tool with a high degree of reliability and validity. In this sense, the evaluation tool for sexuality education developed by this study can be effectively utilized in Korea elementary schools.

• 식품기술
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• 제22권2호
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• pp.247-254
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• 2009

• Biomolecules & Therapeutics
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• 제28권1호
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• pp.1-17
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• 2020

Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells that exert suppressive function on the immune response. MDSCs expand in tumor-bearing hosts or in the tumor microenvironment and suppress T cell responses via various mechanisms, whereas a reduction in their activities has been observed in autoimmune diseases or infections. It has been reported that the symptoms of various diseases, including malignant tumors, can be alleviated by targeting MDSCs. Moreover, MDSCs can contribute to patient resistance to therapy using immune checkpoint inhibitors. In line with these therapeutic approaches, diverse oligonucleotide-based molecules and small molecules have been evaluated for their therapeutic efficacy in several disease models via the modulation of MDSC activity. In the current review, MDSC-targeting oligonucleotides and small molecules are briefly summarized, and we highlight the immunomodulatory effects on MDSCs in a variety of disease models and the application of MDSC-targeting molecules for immuno-oncologic therapy.

• BMB Reports
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• 제55권2호
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• pp.65-71
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• 2022

Regenerative medicine is a research field that develops methods to restore damaged cell or tissue function by regeneration, repair or replacement. Stem cells are the raw material of the body that is ultimately used from the point of view of regenerative medicine, and stem cell therapy uses cells themselves or their derivatives to promote responses to diseases and dysfunctions, the ultimate goal of regenerative medicine. Stem cell-derived extracellular vesicles (EVs) are recognized as an attractive source because they can enrich exogenous microRNAs (miRNAs) by targeting pathological recipient cells for disease therapy and can overcome the obstacles faced by current cell therapy agents. However, there are some limitations that need to be addressed before using miRNA-enriched EVs derived from stem cells for multiplexed therapeutic targeting in many diseases. Here, we review various roles on miRNA-based stem cell EVs that can induce effective and stable functional improvement of stem cell-derived EVs. In addition, we introduce and review the implications of several miRNA-enriched EV therapies improved by multiplexed targeting in diseases involving the circulatory system and nervous system. This systemic review may offer potential roles for stem cell-derived therapeutics with multiplexed targeting.

• Molecules and Cells
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• 제45권11호
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• pp.789-791
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• 2022

Targeting fibroblast growth factor receptors (FGFRs) has been slow compared to other targeted cancer therapies for receptor tyrosine kinases, such as epidermal growth factor receptors. The low efficacy and variable response have limited the growth of FGFR inhibitors in clinical use. Nevertheless, recent systematic and genomic approaches have identified the biological conditions for effectively targeting FGFRs and can accelerate the development of targeted drugs. Under clinical and preclinical trials, the inhibitors started fast growing furious races to target FGFRs. Finally, FGFRs will be more actionable and targetable with more precise and effective drugs at the end of the race, passing the finish line.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2006년도 한국컴퓨터종합학술대회 논문집 Vol.33 No.1 (D)
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• pp.22-24
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• 2006

유선 환경의 초고속 인터넷 서비스는 일반적으로 PC와 같은 고정형 단말을 대상으로 한다. 휴대폰이나 네비게이션용 GPS 단말 같은 이동 단말은 비교적 정확하게 단말의 위치를 찾을 수 있어 이를 활용한 응용 서비스가 확대되는 추세이다. 유선 인터넷 단말의 경우 IP주소의 할당 내역을 바탕으로 비교적 넓은 영역의 지역 정보를 찾아서 이용하는 Geo Targeting이 미국 등 해외에서 활성화 되고 있으나 국내에서는 IP에 대한 지역정보를 얻을 수 있는 적절한 방법이 없어 관련 서비스가 크게 활성화되고 있지 못하다. 본 논문에서는 유선 인터넷 환경 하의 Geo Targeting 구현에 대해 설명하고 이를 활용한 응용 서비스를 소개한다.