• Fisheries and Aquatic Sciences
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• v.19 no.10
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• pp.40.1-40.14
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• 2016

Background: Due to the paucity of oceanic resources utilized in the preparation of diets for cultured fish, commercial feed producers have been trying to replace fishmeal (FM) using alternative protein sources such as vegetable protein meals (VMs). One of the main drawbacks of using VMs in fish feed is related to the presence of a variety of anti-nutritional factors, which could trigger an inflammation process in the distal intestine. This reduces the capacity of the enterocytes to absorb nutrients leading to reduced fish growth performances. Methods: We evaluated the mitigating effects of butyrate and taurine used as feed additives on the morphological abnormalities caused by a soybean meal (SBM)-based diet in the distal intestine of sea bass (Dicentrarchus labrax). We used three experimental diets, containing the same low percentage of FM and high percentage of SBM; two diets were supplemented with either 0.2% sodium butyrate or taurine. Histological changes in the intestine of fish were determined by light and transmission electron microscopy. Infiltration of $CD45^+$ leucocytes in the lamina propria and in the submucosa was assessed by immunohistochemistry. We also quantified by One-Step Taqman$^{(R)}$ real-time RT-PCR the messenger RNA (mRNA) abundance of a panel of genes involved in the intestinal mucosa inflammatory response such as $TNF{\alpha}$ (tumor necrosis factor alpha) and interleukins: IL-8, IL-$1{\beta}$, IL-10, and IL-6. Results: Fish that received for 2 months the diet with 30% soy protein (16.7% SBM and 12.8% full-fat soy) developed an inflammation in the distal intestine, as confirmed by histological and immunohistochemistry data. The expression of target genes in the intestine was deeply influenced by the type of fish diet. Fish fed with taurine-supplemented diet displayed the lowest number of mRNA copies of IL-$1{\beta}$, IL-8, and IL-10 genes in comparison to fish fed with control or butyrate-supplemented diets. Dietary butyrate caused an upregulation of the $TNF{\alpha}$ gene transcription. Among the quantified interleukins, IL-6 was the only one to be not influenced by the diet. Conclusions: Histological and gene expression data suggest that butyrate and taurine could have a role in normalizing the intestinal abnormalities caused by the SBM, but the underling mechanisms of action seem different.

• Journal of Digital Convergence
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• v.20 no.2
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• pp.299-309
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• 2022

YouTube videos secure the latest trends in the field and provide viewers' preferences and non-preferences. This study analyzes the discussion of death in recent YouTube videos and explores the relationship between life, well-dying, and well-aging. The purpose of this study is to contribute to the establishment of a healthy life for Koreans by exploring relevance. The survey period and target are 13 videos uploaded on YouTube from 2019 to 2021. As a result of the investigation, it was possible to encompass 25 speakers who were discussing death centered on doctors. Video speakers in charge of various areas conducted various discussions on death from the perspective of experts. The key points are the true value of life, death that you don't need to fear, the importance of hope, the naturalness of death, etc. 11 expert perspectives are measured into 4 types based on 4 criteria: life, death, well-aging, and well-dying. Despite the limitations of studying 13 extracted at a specific time, this study revealed that YouTube videos are a place for free communication. It can contribute in part to maintaining and improving the health of our lives in the future and researching convergence.

• Journal of Korea Society of Industrial Information Systems
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• v.16 no.1
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• pp.31-36
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• 2011

Ubiquitous network and wireless sensor networks is being applied in various fields. Located at target areas, node of wireless sensor network uses batteries as a power source. Batteries have a limited energy in sensor network applications. Also, before use, the battery must be charged and It is difficult to replace the battery. Therefore, energy harvesting technology is being researched and being developed for long life of sensor node. Especially, sola energy is being extensively researched. because that can have great amounts of energy than other environmental energy in a short time. In this study, we tested battery charging and recharging, operation of sensor node using Solar Cell. Also, monitoring data gathering and voltage Analysis showed energy harvesting time of Sola Cell battery for sensor node and operation of sensor node.

• Journal of Industrial Technology
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• v.27 no.B
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• pp.235-243
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• 2007

Sensor network is used to obtain sensing data in various area. The interval to sense the events depends on the type of target application and the amounts of data generated by sensor nodes are not constant. Many applications exploit long sensing interval to enhance the life time of network but there are specific applications that requires very short interval to obtain fine-grained, high-precision sensing data. If the number of nodes in the network is increased and the interval to sense data is shortened, the amounts of generated data are greatly increased and this leads to increased amount of packets to transfer to the network. To transfer large amount of packets fast, it is necessary that the delay between successive packet transmissions should be minimized as possible. In Sensor network, since the Operating Systems are worked on the event driven, the Timer Event is used to transfer packets successively. However, since the transferring time of packet completely is varies very much, it is very hard to set appropriate interval. The longer the interval, the higher the delay and the shorter the delay, the larger the fail of transfer request. In this paper, we propose ESTEO which reduces the delay between successive packet transmissions by using SendDone Event which informs that a packet transmission has been completed.In ESTEO, the delay between successive packet transmissions is shortened very much since the transmission of next packet starts at the time when the transmission of previous packet has completed, irrespective of the transmission timee. Therefore ESTEO could provide high packet transmission rate given large amount of packets.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.26 no.2
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• pp.99-115
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• 2023

Purpose: Exclusive breastfeeding promotes gut microbial compositions associated with lower rates of metabolic and autoimmune diseases. Its cessation is implicated in increased microbiome-metabolome discordance, suggesting a vulnerability to dietary changes. Formula supplementation is common within our low-income, ethnic-minority community. We studied exclusively breastfed (EBF) neonates' early microbiome-metabolome coupling in efforts to build foundational knowledge needed to target this inequality. Methods: Maternal surveys and stool samples from seven EBF neonates at first transitional stool (0-24 hours), discharge (30-48 hours), and at first appointment (days 3-5) were collected. Survey included demographics, feeding method, medications, medical history and tobacco and alcohol use. Stool samples were processed for 16S rRNA gene sequencing and lipid analysis by gas chromatography-mass spectrometry. Alpha and beta diversity analyses and Procrustes randomization for associations were carried out. Results: Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria were the most abundant taxa. Variation in microbiome composition was greater between individuals than within (p=0.001). Palmitic, oleic, stearic, and linoleic acids were the most abundant lipids. Variation in lipid composition was greater between individuals than within (p=0.040). Multivariate composition of the metabolome, but not microbiome, correlated with time (p=0.030). Total lipids, saturated lipids, and unsaturated lipids concentrations increased over time (p=0.012, p=0.008, p=0.023). Alpha diversity did not correlate with time (p=0.403). Microbiome composition was not associated with each samples' metabolome (p=0.450). Conclusion: Neonate gut microbiomes were unique to each neonate; respective metabolome profiles demonstrated generalizable temporal developments. The overall variability suggests potential interplay between influences including maternal breastmilk composition, amount consumed and living environment.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1518-1521
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• 2008

Bacteria sense their population density and coordinate the expression of target genes, including virulence factors in Gram-negative bacteria, by the N-acylhomoserine lactones (AHLs)-dependent quorum sensing (QS) mechanism. In contrast, several soil bacteria are able to interfere with QS by enzymatic degradation of AHLs, referred to as quorum quenching. A potent AHL-degrading enzyme, AiiA, from Bacillus thuringiensis has been reported to effectively attenuate the virulence of bacteria by quorum quenching. However, little is known about the role of AiiA in B. thuringiensis itself. In the present study, an aiiA-defective mutant was generated to investigate the role of AHA in rhizosphere competence in the root system of pepper. The aiiA mutant showed no detectable AHL￢-egrading activity and was less effective for suppression of soft-rot symptom caused by Erwinia carotovora on the potato slice. On the pepper root, the survival rate of the aiiA mutant significantly decreased over time compared with that of wild type. Interestingly, viable cell count analysis revealed that the bacterial number and composition of E. carotovora were not different between treatments of wild type and the aiiA mutant. These results provide evidence that AHA can play an important role in rhizosphere competentce of B. thuringiensis and bacterial quorum quenching to Gram-negative bacteria without changing bacterial number or composition.

• Journal of Society of Preventive Korean Medicine
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• v.3 no.2
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• pp.91-100
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• 1999

Today, toxicology is used for many purpose, in many fields. Classification of special toxic effect is related next 4 important principles. 1. The chemical substance must move to target organ or tissue that can induce Biological effect. For this movement, we have to understand the physical-chemical characteristic of substance, and the rout of absorption, metabolism, diffusion and excretion of toxic substance. 2. Every biological effect that induced by chemical substance is not harmful. For example, some specific chemical substance is not harmful in liver enzyme system. 3. The strength of biological effect induced by chemical substance is deep related with dose. Nearly all substance is not effective below the specific dose, and it may toxic to death over the specific dose. It is the 'Dose - response relationship' But carcinogen may toxic whether it is law dose or not. 4. The information that was obtained by experimental animal test, could have to adapt in human biology. Because biological effect of chemical substance could be different in every biological species. In past, drugs was obtained by animal or plants. But in the future, it could be obtained by biochemistry, and genome project. Therefore, in Oriental medicine, research and approach is needed at this time, and have to develop new method of experience in toxic method.

• Journal of Electrical Engineering and Technology
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• v.12 no.4
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• pp.1406-1416
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• 2017

In complex and large-scale industries, properly designed fault detection and identification (FDI) systems considerably improve safety, reliability and availability of target processes. In thermal power plants (TPPs), generating units operate under very dangerous conditions; system failures can cause severe loss of life and property. In this paper, we propose a bagged auto-associative kernel regression (AAKR)-based FDI approach for steam boilers in TPPs. AAKR estimates new query vectors by online local modeling, and is suitable for TPPs operating under various load levels. By combining the bagging method, more stable and reliable estimations can be achieved, since the effects of random fluctuations decrease because of ensemble averaging. To validate performance, the proposed method and comparison methods (i.e., a clustering-based method and principal component analysis) are applied to failure data due to water wall tube leakage gathered from a 250 MW coal-fired TPP. Experimental results show that the proposed method fulfills reasonable false alarm rates and, at the same time, achieves better fault detection performance than the comparison methods. After performing fault detection, contribution analysis is carried out to identify fault variables; this helps operators to confirm the types of faults and efficiently take preventive actions.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.4 no.1
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• pp.3-10
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• 2018

The folate receptor (FR) is a promising cell membrane-associated target for nuclear imaging of various cancers (via imaging $FR-{\alpha}$) and potentially also inflammatory diseases (via imaging $FR-{\beta}$), through the use of folic acid-based radioconjugates. However, there have been several drawbacks of previously reported radioconjugates, such as a short half-life of the radiolabel ($^{68}Ga\;t_{1/2}$ 68 min), a complex and time-consuming multistep radiosynthesis, and a high renal uptake of radiolabeled folate derivatives. The goal of this study was to develop an imaging probe by directly labeling folate with radioactive iodine without using an extra prosthetic group. The radiolabeling of folate was optimized using various labeling conditions and the labeled tracers were isolated by high-performance liquid chromatography. The in vitro stability of labeled folate was checked in phosphate-buffered saline and serum. The tumor-targeting efficacy of the probe was also evaluated by biodistribution studies using a murine 4T1 tumor model.

• Journal of the Korea Convergence Society
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• v.8 no.10
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• pp.311-321
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• 2017

In recent years, awareness of talent donation has been increasing as a way to lead a happy life with others. The purpose of this study is to investigate participation characteristics such as participation difficulties, participation period, service target, participation field, participation style, participation motive of beauty talented volunteers. This survey responded to 701 beauty talent volunteers. Participation difficulties included lack of skills and capability, lack of social awareness, and lack of spare time. Participation characteristics varied according to gender, age, education, occupation, monthly income, etc. Based on the results of these researches, the suggestions for activation for donating beauty talents were presented.