• Journal of Digital Contents Society
• /
• v.19 no.2
• /
• pp.293-300
• /
• 2018

This study explored the security of Industry 4.0 such as security trends and security threats in Industry 4.0, and security system in Industry 4.0. The threat elements in Industry 4.0 are changing from ICT to IoT and to CPS security, so security paradigm and security System should change accordingly. In particular, environmental and administrative security are more important to solve CPS security. The fourth industry-age security should change to customized security for individual systems, suggesting that the security technology that combines hardware and software in product production design should change from the beginning of development. The security system of the fourth industry proposes design and implementation as a CPS security system as a security system that can accommodate various devices and platforms from a security system in a single system such as a network to an individual system.

• Korean Journal of Food Science and Technology
• /
• v.50 no.4
• /
• pp.444-450
• /
• 2018

Dendritic cells (DCs) are potent antigen-presenting cells that play a pivotal role in modulating both innate and adaptive immunity. This study examined the immunomodulatory activities of hot-water extracts of bee pollen (BPW) in bone-marrow derived DCs (BMDC) and mice splenocytes. BMDCs isolated from mice were treated with 250 and $500{\mu}g/mL$ BPW for 24 h. BPW, up to $500{\mu}g/mL$, did not display any cellular toxicity against BMDCs. In fact, it functionally induced BMDC activation via augmentation of CD80, CD86, and major histocompatibility complex (MHC) class I/II expression and pro-inflammatory cytokine (tumor necrosis factor; $TNF-{\alpha}$, interleukin; IL-6, and $IL-1{\beta}$) production. Interestingly, BPW treatment significantly increased the production of interferon $(IFN)-{\gamma}$ in splenocytes, suggesting its possible contribution to Th1 polarization in immune response. Taken together, these findings suggest that BPW may regulate innate and adaptive immunity via DC activation and Th1 polarization in immune responses.

• Journal of Life Science
• /
• v.21 no.12
• /
• pp.1761-1771
• /
• 2011

In the previous results, we developed an effective products to apply as functional foods for overcome of radiation damage and reduction of side effects in radiotherapy. To verify the prevention of UVB-induced immunosuppression of immune cell function by HemoHIM, we studied on the mechanism of the skin immune function for the protection in UVB irradiation. In studies presented here, we showed that HemoHIM can prevent UVB-induced impairment of skin immune cell function by in vitro and in vivo assay. Exposure of freshly cultured murine dendritic cells (DCs) with IL-4/GM-CSF to UVB irradiation resulted in impairment of accessory function. This suppression could be prevented by addition of HemoHIM before or after to the cultures of UVB-irradiated DCs. We also tested the effects of HemoHIM on the suppression of contact hypersensitivity (CHS) treated oral or intraperitoneal administration. This UVB-suppressed CHS was prevented by administration of HemoHIM to UVB-irradiated mice. These results suggest that HemoHIM may prevent UVB-induced immune suppression in the skin.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.1
• /
• pp.160-170
• /
• 2019

The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

• BMB Reports
• /
• v.47 no.2
• /
• pp.115-120
• /
• 2014

In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bone marrow-derived dendritic cells (BMDCs), a representative antigen presenting cell (APC). MAP1305 protein induces DC maturation and the production of pro-inflammatory cytokines (Interleukin (IL)-6), tumor necrosis factor (TNF)-${\alpha}$, and IL-$1{\beta}$) through Toll like receptor-4 (TLR-4) signaling by directly binding with TLR4. MAP1305 activates the phosphorylation of MAPKs, such as ERK, p38MAPK, and JNK, which is essential for DC maturation. Furthermore, MAP1305-treated DCs transform naive T cells to polarized $CD4^+$ and $CD8^+$ T cells, thus indicating a key role for this protein in the Th1 polarization of the resulting immune response. Taken together, M. avium subsp. paratuberculosis MAP1305 is important for the regulation of innate immune response through DC-mediated proliferation of $CD4^+$ and $CD8^+$ T cells.

• Journal of Life Science
• /
• v.17 no.7 s.87
• /
• pp.948-955
• /
• 2007

Neutrophils play a key role as a first line of defense and are known to acquire the characteristics of dendritic cells (DCs) under the appropriate conditions. The spontaneous apoptosis of neutrophils was delayed by treatment with 4-(2-aminoethyl) benzensulfonylfluoride (AEBSF), a serine protease inhibitor. AEBSF inhibited both caspase-3 and serine protease activities, whereas ZVAD-fmk, a pancaspase inhibitor, inhibited only caspase-3 activity. The life span of neutrophils was prolonged up to 5 days by AEBSF in the presence or absence of granulocyte macrophage colony stimulating factor(CM-CSF). DC surface markers, such as CD80, CD83, and MHC class ll were not expressed on neutrophils treated with AEBSF alone. CM-CSF failed to prolong the survival time of neutrophils up to3 days but increased the expression levels of DC markers on neutrophils in the presence of AEBSF. Expression levels of DC markers were the highest on neutrophils treated with CM-CSF and AEBSF for 3 days. AEBSF and CM-CSF-treated neutrophils stimulated proliferation of T cells in the presence of a superantigen, Staphylococcal enterotoxin B (SEB) but produced $interferon-{\gamma}$ ($IFN{\gamma}$) in the absence of SEB. These results suggest that the inhibition of serine protease activity prolonged the life span of human neutrophils and combined treatment of neukophils with CM-CSF and serine protease inhibitor induced differentiation of neutrophils into DC-like cells.

• Journal of Digital Contents Society
• /
• v.12 no.2
• /
• pp.253-262
• /
• 2011

IT skills might be just a product for people without disabilities; however it can be "another sense" that is able to give the disabled the same social abilities as non-disabled people. Library services are necessary to help disabled people have access to information. The effectiveness of the library services for the disabled depends on the level and the size of library. If 10% of the total domestic population couldn't participate in their social activities, it must be a huge individual at the same time national loss. This paper will look into supporting technologies and services for the reading disabled through literature researches and visiting domestic and international libraries. Based on this analysis, this paper suggests development plans for enhancing domestic situation. We are sure that our suggestions can contribute to give a long-term service development strategy for improving the reading disabled people's information accessibility.

• Journal of Digital Contents Society
• /
• v.12 no.4
• /
• pp.567-574
• /
• 2011

In this paper, we have designed and implemented a commercial web system for the future which resolves the necessary security and compatibility to the existing issues in various fields such as theater or the ball park that tickets are issued. The system presents the OTP (One Time Password) system using the mobile in order for defense in the bypass hacking technique such as screen hacking and for greater security. Also, we presents a smart ticketing system that improve the existing responsive hybrid web. It uses Non-ActiveX System which solve compatible problems with present systems that don't use ActiveX. Also, it is available in a variety of environments and equipment. In addition, the system provides an intelligent screen switching systems using the characteristics of mobile devices, an automatic discount system, and a venue information system which shows the shortest distance to the venue considering the commercial portion.

• Journal of Digital Contents Society
• /
• v.18 no.1
• /
• pp.1-7
• /
• 2017

Virtual reality(VR) is known as immersive multimedia or computer-simulated reality, is a computer technology that replicates an environment, real or imagined, and simulates a user's physical presence and environment to allow for user interaction. Virtual realities artificially create sensory experience including sight, touch, hearing, and smell. Owing to the use of a single device in most VR contents, user have difficulty in manipulating user interface and game object. And also immersion of the game goes down because they can't see the mouse and keyboard in virtual space. In this paper, we design and implement the chess game to easily and accurately control user interface to improve the immersion in game.

• Journal of Digital Contents Society
• /
• v.18 no.7
• /
• pp.1393-1401
• /
• 2017

The Linux operating system is expanding to include desktops and servers, as well as mobile devices, automobiles and embedded devices, and is the dominant operating system category because it is freely available and open source software. However, the desktop market is dominated by Microsoft's Windows, and Apple's Mac OS is increasingly dominated by enthusiasts, but Linux's growth is stagnant. An important element to make up for this deficiency is the X Window System. Most Linux distributions still use the traditional X.org server as the X Window System, but it is heavy and slow. To replace it, a new X window system called Wayland emerged. Wayland is small and light enough to fit on an embedded or mobile device, so it can be mounted on the IoT related device as well as on the desktop. With the advent of Wayland, the change in the X window will accelerate the popularization of Linux.