• Genomics & Informatics
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• v.16 no.1
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• pp.2-9
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• 2018

Olfactory receptors (ORs) in mammals are generally considered to function as chemosensors in the olfactory organs of animals. They are membrane proteins that traverse the cytoplasmic membrane seven times and work generally by coupling to heterotrimeric G protein. The OR is a G protein-coupled receptor that binds the guanine nucleotide-binding $G{\alpha}_{olf}$ subunit and the $G{\beta}{\gamma}$ dimer to recognize a wide spectrum of organic compounds in accordance with its cognate ligand. Mammalian ORs were originally identified from the olfactory epithelium of rat. However, it has been recently reported that the expression of ORs is not limited to the olfactory organ. In recent decades, they have been found to be expressed in diverse organs or tissues and even tumors in mammals. In this review, the expression and expected function of olfactory receptors that exist throughout an organism's system are discussed.

• International Journal of Industrial Entomology and Biomaterials
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• v.23 no.1
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• pp.147-153
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• 2011

The Pseudorabies (PR), also called Aujeszky's disease (AD), is an infectious viral disease caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. Pseudorabies virus (PRV) virions contain several envelope glycoproteins. Among them, gB, gC and gD are regarded as the major immunogenic proteins. We expressed these glycoproteins using the bacterial expression system and analyzed recombinant proteins. Expression of glycoproteins gC and gD were observed on SDS-PAGE or Western blot analysis, but gB was not. Optimal concentration of IPTG and inducing time were determined as 1.0 mM and 4 h, respectively, for the expression of both gC and gD in E. coli. A sodium dodecyl sulfate (SDS) was the most efficient detergent in solubilizing insoluble recombinant protein.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.281-284
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• 2003

Development of internet had centered on that offer universality of expression. But, graphic processing of expression center has many inconveniences because of method that is not structural in World Wide Web. Announced SVG that is XML application to permit efficient expression and storage of vector graphic expression, share in W3C to supplement these things. Therefore, because using SVG document in this treatise, edit vector graphic through interface that could edit expressed graphic object directly structural edit and screen that is offered in graphic edit system form and designed SVG edit system to express. This system may use usefully to efficient XML graphics processing on the GIS system and Mobile service environment and so on.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.463-469
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• 2002

Rotaviruses are the world-wide leading causative agents of severe dehydrating gastroenteritis in young children and animals. The outer capsid glycoprotein VP7 and inner capsid glycoprotein VP6 of rotaviruses are highly antigenic and immunogenic. An SFV-based expression system has recently emerged as a useful tool for heterologous protein production in mammalian cells, exhibiting a much more efficient performance compared to other gene expression systems. Accordingly, the current study adopted an SFV-based expression system to express the VP7 of a group A human rotavirus from a Korean isolate, and the VP6 of a group B bovine rotavirus from a Korean isolate, in mammalian cells. The genes of the VP6 and VP7 were inserted into the SFV expression vector pSFV-1. The RNA was transcribed in vitro from pSFV-VP6 and pSFV-VP7 using SP6 polymerase. Each RNA was then electroporated into BHK-21 cells along with pSFV-helper RNA containing the structural protein gene without the packaging signal. The expression of VP6 and VP7 in the cytoplasm was then detected by immunocytochemistry. The recombinant virus was harvested by ultracentrifugation and examined under electron microscopy. After infecting BHK-21 cells with the defective viruses, the expressed proteins were separated by SDS-PAGE and analyzed by a Western blot. The results indicate that an SFV-based expression system fur the VP6 and VP7 of rotaviruses is an efficient tool for developing a diagnostic kit and/or preventive vaccine.

• The Plant Pathology Journal
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• v.18 no.2
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• pp.57-62
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• 2002

In vivo expression technology (IVET) has been developed to study bacterial gene expression in Salmonella typhimurium during host infection. The expression of selected genes by IVET has been elevated in vivo but not in vitro. The selected genes turned out to be important for bacterial virulence and/or pathogenicity. IVET depends on a synthetic operon with a promoterless transcriptional fusion between a selection marker gene and a reporter gene. The IVET approach has been successfully adapted in other bacterial pathogens and plant-associated bacteria using different selection markers. Pseudomonas putida suppresses citrus root rot caused by Phytophthora parasitica and enhances citrus seedling growth. The WET strategy was adapted based on a transcriptional fusion, pyrBC'-lacZ, in P. putida to study the bacterial traits important far biocontrol activities. Several genes appeared to be induced on P. parasitica hyphae and were found to be related with metabolism and regulation of gene expression. It is likely that the biocontrol strain took a metabolic advantage from the plant pathogenic fungus and then suppressed citrus root rot effectively. The result was parallel with those from the adaptation of IVET in P. fluorescens, a plant growth promoting rhizobacteria (PGPR). Interestingly, genes encoding components for type III secretion system have been identified as rhizosphere-induced genes in the PGPR strain. The type III secretion system may play a certain role during interaction with its counterpart plants. Application of IVET has been demonstrated in a wide range of bacteria. It is an important strategy to genetically understand complicated bacterial traits in the environment.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.4
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• pp.798-803
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• 2004

XML(extensible Markup Language) of W3C(World Wide Web Consortium) that is used the standard core technology of data exchange on the current Internet is an independent data type of usable at the all platforms. Especially, it can handle rapidly because of the integration of each other data types that is exchanged. Between each application and system that built at an enterprise in the past. However, W3C had notice to use XSLT(extensible Stylesheet Language Transformation) that is document transformable standard to descript expression information in XML documents because documents of XML only have a logical structure information. It is designed for XML that is developed for data exchange on the internet. Moreover, it is proposed to process and to change as other data type for expression XML documents for user. This thesis design and implement XSLT document editing system transformable as a un data type as a HTML data type applying XSLT at XML and developed the system. It can edit XSLT document that descript expression information in XML document that is used for data editing in the WYSIWYG environment.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05b
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• pp.212-216
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• 2004

XML(extensible Markup Language) of W3C(World Wide Web Consortium) that is used the standard core technology of data exchange on the current Internet is an independent data type of usable at the all platforms. Especially, it can handle rapidly because of the integration of each other data types that is exchanged. Between each application and system that built at an enterprise in the past. However, W3C had notice to use XSLT(extensible Stylesheet Language Transformation) that is document transformable standard to descript expression information in XML documents because documents of XML only have a logical structure information. It is designed for XML that is developed for data exchange on the internet. Moreover, it is proposed to process and to change as other data type for expression XML documents for user. Tris thesis design and implement XSLT document editing system transformable as a HTML data type applying XSLT at XML and developed the system. It can edit XSLT document that descript expression information in XML document that is used for data editing in the WYSIWYG environment.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1357-1363
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• 2008

The $P_{entC}$ promoter of the entCERA operon encoding enzymes for enterobactin biosynthesis in Escherichia coli is tightly regulated by the availability of iron in the culture medium. In iron-rich conditions, the $P_{entC}$ promoter activity is strongly repressed by the global transcription regulator Fur (ferric uptake regulator), which complexes with ferrous ions and binds to the Fur box 19-bp inverted repeat. In this study, we have constructed the expression vector pOS2 containing the $P_{entC}$ promoter and characterized its repression, induction, and modulation by quantifying the expression of the lacZ reporter gene encoding $\beta$-galactosidase. $\beta$-Galactosidase activities of E. coli transformants harboring pOS2-lacZ were highly induced in the presence of divalent metal ion chelators such as 2,2'-dipyridyl and EDTA, and were strongly repressed in the presence of excess iron. It was also shown that the basal level $\beta$-galactosidase expression by the $P_{entC}$ promoter was drastically decreased by incorporating the fur gene into the expression vector. Since the newly developed iron chelator-inducible expression system is efficient and cost-effective, it has wide applications in recombinant protein production.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.6
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• pp.1174-1181
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• 2005

The exact bit error rate(BER) calculation of an UWB-TH PPM multiple access communication system, which is known to be suitable for the fast transmission of massive information data, is introduced. The statistic feature of the multiple access intereference (MAI) of the system is precisely modeled by the characteristic function technique. The concrete expression for the MAI allows the exact expression for BER to be derived. In addition, we propose the approximate expression for the BER which reveals superior accuracy to the expression from the previous Gaussian approximation of the MAI. The validity of the proposed expressions is confirmed from the comparison of proposed results with the results from Monte-Carlo simulation.

• Proceedings of the IEEK Conference
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• 2003.07a
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• pp.446-449
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• 2003

In recent years, the research of an efficient transmission method is going to meet a demand for high speed and large capacity radio communication. These systems make use of ultra-short duration pulses which yield UWB(Ultra Wide Band) signals characterized by low power spectral densities. The wavelet synthesis wave is able to set up the scale freely. So it is possible to use as the transmission wave of UWB by compressing time. In this paper, we present a general analytical expression for the average BER(Bit Error Rate) performance of UWB data transmission using wavelet system as a function of the cross-correlation between the users' signatures in an additive white Gaussian noise(AWGN) channel.