• KSBB Journal
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• 제17권1호
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• pp.110-113
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• 2002

본 연구에서는 고려인삼세포 현탁배양에 의한 회분배양 실험에서 쟈스몬산과 안식향산이 ginsenoside 생산에 미치는 영향을 조사하였다. 세포내에서 이차대사계를 활성화시키는 중간 신호전달 물질(signal transducer)로 알려진 쟈스몬산은 50 $\mu$M 이상의 농도로 투여되었을 때 ginsenoside 생산을 증가시킬 수 있었다. 그 이상의 농도에서는 세포 생장을 저하시키는 결과를 보였다. 쟈스몬산 투여 후 12시간가지 ginsenoside의 생산은 증가하였고, 그 이후에는 더 이상 증가하지 않았다. 안식향산과 쟈스몬산을 동시에 투여하면 ginsenoside 생산이 9.6배 증가하는 결과를 나타내었다. 이는 쟈스몬산 단독투여 때보다 2.2배 증가된 결과였다.

• Archives of Pharmacal Research
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• 제17권1호
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• pp.54-55
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• 1994

• Journal of Microbiology and Biotechnology
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• 제17권12호
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• pp.1944-1948
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• 2007

Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at $60^{\circ}C$ for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could not elevate cell viability at the concentrations up to 3% (v/v), addition of 0.3% (v/v) SH to a culture medium enhanced the production of hCTLA4Ig by 36.8% over an SH-free medium. Moreover, the production period of hCTLA4Ig could be shortened in a 0.3% (v/v) SH-added medium compared with that in an SH-free culture. As a result, addition of 0.3% (v/v) SH improved the productivity of hCTLA4Ig significantly in transgenic rice cell cultures.

• KSBB Journal
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• 제11권5호
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• pp.600-605
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• 1996

Taxus brevifolia와 Taxus cuspidata 현탁세포배 양에서 taxa!의 생산에 가장 적합한 초기 접종농도 를 결정하기 위해 접종농도를 세포생체중량으로 2.5, 5 5, 7.5, 10 g/flask가 되도록 변화시켜 세포의 생장 과 taxol의 생산을 측정하였다. Taxus brevifolia의 경우 초기 접종농도가 높을수록 lag phase가 감소되 는 경향을 보였으며, 두 가지 세포주(Taxus bre­1 vifolia와 Taxus cuspidata)에서 모두 초기 접종농도 를 5 g/flask가 taxol의 생산에 최척 임을 확인할 수 있었다. Taxol 생산에 가장 척합한 초기 접종농도로 생장배지와 생산배지에 세포를 접종시켰을 때 생장 배지에서는 taxal이 검출되지 않았지만 6% 자당을 이용한 생산배지에서는 9일째 taxol이 최대로 생산 되었다가 이후 다시 줄어드는 경향을 보였다.

• Journal of Plant Biotechnology
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• 제6권4호
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• pp.265-268
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• 2004

Cell growth and ginseng saponin production by large-scale suspension (bioreactor) cultures of Panax ginseng were investigated under various inoculum sizes. Cell growth was low at an inoculum size of 40 g FW/L, and the maximum cell growth was obtained with increasing inoculum size up to 100 g FW/L. The cell density of 333 g FW/L and 12.7 g DW/L was obtained at inoculum size of 100 g FW/L after 30 days of cultivation. Maximum saponin production of $4.40\;\cal{mg/g}$ DW was achieved at 60 g FW/L of inoculum size. Thus, inoculum size 60 g FW/L was suitable for optimum biomass accumulation as well as saponin production during bioreactor cultivation of ginseng suspension cells.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.101-106
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• 2001

In order to establish efficient plant regeneration from embryogenic suspension cultures of soybean, Glycine max L, we examined the effects of auxin type and concentration, cytokinin type and concentration, and amino acid type and concentration on the growth of embryogenic clumps from induced callus, and the effect of desiccation of mature somatic embryos obtained from these clumps on the frequency of somatic embryo germination. Embryogenic callus was induced from the edge of the cotyledons cultured on MS medium containing 6% sucrose, 40 mg/L 2,4-D, 0.2% gelrite and pH 5.7. The growth of embryogenic clumps was best in early staged, embryogenic callus that was placed in suspension culture of MS medium containing 5 mg/L 2,4-D and 0.5 mg/L asparagine. Single somatic embryos were isolated from the clumps and plated on the same medium for maturation. When the mature single somatic embryos were desiccated for 96 h, somatic embryo germination came up to approximately 90%. The plantlets germinated after embryos desiccation for 2 weeks were transfered to MS medium containing 3% sucrose,0.2% gelrite and pH 5.7.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권1호
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• pp.51-55
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• 2001

This study was conducted to establish a plant cell culture system for the production of medically important secondary metabolites from Xanthium strumarium. The effects of plant growth regulators including NAA, 2,4-D, kinetin, and ABA were examined in terms of callus induction, maintenance of callus and suspension cultures. It was shown that callus was induced upon treatment with NAA while embryo was induced after treatment with 2,4-D. Callus formation was further improved by treatment with ABA and NAA. The level of callusing increased by 17-29% for the seed case, cotyledon, leaf, and hypocotyl and by 96% in the case of the root. Suspension cell lines were established using calli produced from cotyledon, hypocotyl and root and cultured at 25$\^{C}$ under light conditions. The cells grew up to 15g/L with NAA 2ppm, BA 2ppm, and ABA 1ppm treatment. Supernatants of suspension cultures of cell lines derived from coyledon and hypocotyl produced some distinctive secondary metabolites, one of which was identified as 8-epi-tomentosin, which belongs to the xanthanolides. The amounts of 8-epi-tomentosin produced by the cotyledon- and hypocotylderived cell lines were 13.4mg/L and 11.0mg/L, respectively.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.131-135
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• 2003

Callus and cell suspension cultures of Eurycoma longifolia Jack were initiated from leaves of different trees. The leaf explant of tree Eu9 produced the most calli and also induced high cell biomass in the cell suspension culture. Optimum production of cell biomass could be initiated in proliferating culture medium with a pH of 5.75 prior to autoclaving. The effects of macronutrient inorganic salts of Murashige and Skoog (MS) liquid medium supplemented with X on production of cell biomass of Eurycoma longifolia were also investigated. The highest cell biomass was produced in MS medium containing macronutrients of $21\;mM\;NH_4NO_3,\;12.25\;mM\;KNO_3,\;3.00\;mM\;CaCl_2.2H_2O,\;0.575\;mM\;MgSO_4.7H_2O$, and $1.83\;mM\;KH_2PO_4$. A new medium labeled as TAM was formulated for the production of Eurycoma longifolia cell biomass in the cell suspension culture.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.107-112
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• 2001

The kinetics of Ti-transformed Salvia miltiorrhiza cell cultures was studied in 250-$m\ell$ shake flasks by using B5 medium with addition of 30 gfL of sucrose. In the cell cultures, the maximum cell mass obtained was 11.5 g DW/L on day 15. The highest amount of phenolic compounds - rosmarinic acid (RA) and lithospermic acid B (LAB) reached 871.3 mg/L (day 15) and 121.3 mg/L (day 13), respectively. The total tanshinone production, i.e., intracellular plus extracellular cryptotanshinone, tanshinone 1, and tanshinone IIA, was 5.3 mg/L on day 13. For the cultivations in 2.4-L stirred bioreactors, the residual sugar level and medium conductivity were a little higher in a small turbine impeller reactor ($T_s$) than those in a large turbine impeller reactor ($T_L$), while a higher cell density was obtained in the $T_L$. For the production of tanshinones and phenolics, better results were obtained in the $T_L$ than in the $T_s$. In the $T_L$, similar or even a little higher production titers of tanshinones and phenolic compounds were achieved compared to those in the flasks. The results suggest that the shake flask results could be successfully scaled up to the $T_L$ reactor. Such a large impeller reactor like $T_L$ may be better than a small impeller one for the large-scale production of the valuable metabolites by the suspension cultures of Ti transformed S.miltiorrhiza cells. This is considered due to the beneficial culture environment in the $T_L$, such as low shear rates as estimated theoretically.

• KSBB Journal
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• 제21권5호
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• pp.331-335
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• 2006

참당귀(Angelica gigas Nakai) 현탁세포의 면역 증강성 다당의 생산을 증진시키기 위하여 초기 당농도와 생장조절제인 2,4-D의 농도를 변화시켰다. 당의 농도를 높일수록 세포의 생장과 ECP의 생산은 각각 1.6배, 1.1배 증가하여 효과적이었다. 2,4-D는 첨가하지 않은 것이 세포의 생장이 대조군에 비해 1.2배 높았지만, ECP의 생산의 경우 2mg/L 2,4-D를 첨가한 경우 대조군에 비해 10% 이상 더 생산되었다. 또한 배양 중 여러 가지의 elicitor를 첨가함으로써 ECP의 생산을 증진시키기 위하여 fungal elicitor들을 처리한 결과 Verticillium dahliae 90 mg/L을 배양 4일째에 처리한 경우 세포의 생장을 다소 저해시킨 반면, ECP 생산량이 1.7배나 증가함을 확인하였다.