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Effect of Inoculum Size on Biomass Accumulation and Ginsenoside Production by Large-Scale Cell Suspension Cultures of Panax ginseng  

Thanh Nguyen Trung (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Murthy Hosakatte Niranjana (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Department of Botany, Karnatak University)
Yu Kee-Won (CBN Biotech Co. Ltd.)
Jeong Cheol Seung (CBN Biotech Co. Ltd.)
Hahn Eun-Joo (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Paek Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.6, no.4, 2004 , pp. 265-268 More about this Journal
Abstract
Cell growth and ginseng saponin production by large-scale suspension (bioreactor) cultures of Panax ginseng were investigated under various inoculum sizes. Cell growth was low at an inoculum size of 40 g FW/L, and the maximum cell growth was obtained with increasing inoculum size up to 100 g FW/L. The cell density of 333 g FW/L and 12.7 g DW/L was obtained at inoculum size of 100 g FW/L after 30 days of cultivation. Maximum saponin production of $4.40\;\cal{mg/g}$ DW was achieved at 60 g FW/L of inoculum size. Thus, inoculum size 60 g FW/L was suitable for optimum biomass accumulation as well as saponin production during bioreactor cultivation of ginseng suspension cells.
Keywords
Bioreactor; ginseng; inoculum size; saponin; suspension culture;
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1 Matsubara K, Kitani S, Yoshioka T, Morimoto T, Fujita Y, Yamada Y (1989) High-density culture of Coptis japonica cells increases berberine production. J Chem Tech Biotechnol 46: 61-69
2 Rokem JS, Goldberg I (1985) Secondary metabolites from plant cell suspension cultures: Methods for yield improvement. In: Mizrahi A, van Wenzel AL eds. Advances in Biotechnological Processes 4. New York: Alan R. Liss, pp 241-274
3 van Gulik WM, Nuutila AM, Vinke KL, ten Hoopen HJG, Heijen JJ (1994) Effect of carbon dioxide, air flow rate, and inoculation density on the batch growth of Catharanthus roseus cell suspensions in stirred fermentors. Biotechnol Prog 10: 335-339
4 Lian ML, Chakrbarty D, Paek KY (2002) Effect of plant growth regulators and medium composition on cell growth and saponin production during cell suspension culture of mountain ginseng (Panax ginseng C. A. Mayer). J Plant Bioi 45: 201-206
5 Wang HO, Zhong, JJ, Yu JT (1997) Enhanced production of taxol in suspension cultures of Taxus chinensis by controlling inoculum size. Biotechnol Lett 19: 353-355
6 Paek KY, Hahn EJ, Son SH (2001) Application of bioreactors for large-scale micropropagation systems of plants In Vitro Cell Dev. BioI. 37:284-292
7 Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improving ginsenoside accumulation in adventitious root culture of Panax ginseng CA Meyer. Biochem Eng J 11: 211-215
8 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. physiol. Plant. 15: 473-479
9 Ryu, Dewey DY, Lee SO (1990) Determination of growth rate for plant cell cultures: Comparative studies. Biotechnol Bioeng 35: 305-311
10 Ozeki Y, Komamine A (1985) Effects of inoculum density, zeatin and sucrose on anthocynin accumulation in a carrot suspension culture. Plant Cell Tiss Org Cult 5:45-53
11 Fujita Y, Hara Y (1985) The effective production of shikonin by cultures with an increased cell population with an increased cell population. Afric Bioi Chem 49: 2071-2075
12 Su WW, Lei F (1993) Rosmarinic acid production in perfused Anchusa officinalis culture: effect of inoculum size. Biotechnol Lett 15: 1035-1038
13 Sakurai M, Mori T, Seki M, Furusaki S (1996) Changes of anthocyanin composition by conditioned medium and cell inoculum size using strawberry suspension culture. Biotechnol Lett 18: 1149-1154
14 Akalezi CO, Liu S, Li OS, Yu JT, Zhong JJ (1999) Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Process Biochem 34: 639-642