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Effects of Silkworm Hemolymph on Cell Viability and hCTLA4Ig Production in Transgenic Rice Cell Suspension Cultures  

Cheon, Su-Hwan (Department of Biological Engineering, Inha University)
Lee, Kyoung-Hoon (Department of Biological Engineering, Inha University)
Kwon, Jun-Young (Department of Biological Engineering, Inha University)
Ryu, Hyun-Nam (Department of Biological Engineering, Inha University)
Yu, Da-Hyun (Department of Biological Engineering, Inha University)
Choi, Yong-Soo (Department of Biological Engineering, Inha University)
Kim, Dong-Il (Department of Biological Engineering, Inha University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.12, 2007 , pp. 1944-1948 More about this Journal
Abstract
Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at $60^{\circ}C$ for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could not elevate cell viability at the concentrations up to 3% (v/v), addition of 0.3% (v/v) SH to a culture medium enhanced the production of hCTLA4Ig by 36.8% over an SH-free medium. Moreover, the production period of hCTLA4Ig could be shortened in a 0.3% (v/v) SH-added medium compared with that in an SH-free culture. As a result, addition of 0.3% (v/v) SH improved the productivity of hCTLA4Ig significantly in transgenic rice cell cultures.
Keywords
Silkworm hemolymph (SH); transgenic plant cell cultures; hCTLA4Ig; RAmy3D promoter;
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