• Journal of Electrical Engineering and Technology
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• v.8 no.5
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• pp.1169-1174
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• 2013

The objective of this work was to increase the efficiency of ultraviolet-light emitting diodes at 375 nm for sterilization. Since $TiO_2$ had antibacterial properties, which were attributed to the appearance of hydroxyl radicals and superoxide radical anions on the surface species under ultra violet radiation at about 387 nm, photo-reactive layers such as Ag-doped $TiO_2$ were coated on aluminum substrates by electrostatic spraying. Crystallinity and surface morphology of the coating layer were examined by X-ray diffraction ${\theta}-2{\theta}$ scan and field emission-scanning electron microscope, respectively. In an antibacterial test, we observed above 99% reduction of Escherichia coli populations on 3 or 5 mol% Ag-doped $TiO_2$ layers after irradiation for 2 hrs at 375 nm, while very low inactivation on bare aluminum substrates occurred after irradiation as the same condition.

• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1209-1215
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• 2017

Microwave sterilization was performed to inactivate the spores of biofilms of Bacillus cereus involved in foodborne illness. The sterilization conditions, such as the amount of water and the operating temperature and treatment time, were optimized using statistical analysis based on 15 runs of experimental results designed by the Box-Behnken method. Statistical analysis showed that the optimal conditions for the inactivation of B. cereus biofilms were 14 ml of water, $108^{\circ}C$ of temperature, and 15 min of treatment time. Interestingly, response surface plots showed that the amount of water is the most important factor for microwave sterilization under the present conditions. Complete inactivation by microwaves was achieved in 5 min, and the inactivation efficiency by microwave was obviously higher than that by conventional steam autoclave. Finally, confocal laser scanning microscopy images showed that the principal effect of microwave treatment was cell membrane disruption. Thus, this study can contribute to the development of a process to control food-associated pathogens.

• Journal of the Korea Convergence Society
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• v.13 no.3
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• pp.281-289
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• 2022

Plasma ozone is utilized in a variety of applications in the field of sterilization due to its high sterilization performance. Dielectric materials used in DBD(dielectric barrier discharges) are mainly polymer, quartz and ceramics. These dielectric layers have the advantage of limiting the amount of supplied electron charge and allowing plasma to occur evenly on the surface of dielectric. Actually, the target or environment for sterilization is often a complex structure, so research and academic study are needed by utilizing the concept of space sterilization. In this study, the device is applied to generate DBD plasma at atmospheric pressure for disinfection due to the effectiveness in producing radicals and ozone. The generator of plasma ozone is a basic structure of dielectric barrier discharge by placing ceramic tube dielectrics and stainless steel electrical conductors at regular intervals. Various applications can be developed based on the proposed design method. Plasma ozone generation for space sterilization device is recognized as an excellent sterilization device. Through the design and verification of the device, we intend to establish an optimal design of the spatial sterilization device and provide the basis data for sterilization applications.

• The korean journal of orthodontics
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• v.28 no.2 s.67
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• pp.329-341
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• 1998

Sterilization has received much attention in orthodontic practices over the past several years. The present study was undertaken to investigate the effects of sterilization on the physical properties of orthodontic pliers-AEZ, Unitek, and Dentronix ligature cutters. This study was designed to examine the tips of ligature cutters before and after 200 and 400 sterilization cycles using the Bowmar RHT-1000, the Dentronix DDS-5000, and the Eschmann SES-2000. The tip surface and the fracture surface were observed with a scanning electron microscope. The microstructure was observed with an optical microscope. The hardness test was carried out with the mic개-Vickers hardness tester and the Rockwell C Scale hardness tester. The chemical composition was analyzed by energy dispersive X-ray spectrometer. The results of this study were as follows : 1. The number and the size of corrosion products on the tip surface and the proportion of cleavage planes in fractured specimen increased, but the hardness of the tip decreased in proportion to sterilization cycles. From these observations, it was considered that mechanical properities decreased in proportion to sterilization cycles. 2. The number and the size of chromium carbides increased in proportion to sterilization cycles. Coarse microstructure decreased mechanical properities. 3. The AEZ and Unitek ligature cutters were Fe-Cr stainless steels, but the Dentronix ligature cutter was Co-Cr alloy. There were many differences among manufactures, but the chemical composition was .not changed after sterilization cycles. 4. The tip edge of ligature cutter used in a clinic revealed microcracks with the SEM observation. Clinical experience confirmed that ligature cutters were gradually degraded by sterilization.

• Journal of the Korean Society of Radiology
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• v.14 no.5
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• pp.537-543
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• 2020

The elderly population in the modern society is growing rapidly due to advance medical technology and minimally invasive surgery. Therefore, as the tendency to use medical device is increasing, pathogenic infection is a concern. Therefore, the first aim of modern medicine is infection prevention in medical place. Recently, patient implants are increasing using 3D printing. Hydroxyapatite is used as a representative material. And, there haven't had currently absorbed dose standard for sterilization of hydroxyapatite discs. Escherichia coli and Streptococcus mutans contaminated on the surface of hydroxyapatite discs were irradiated at each absorbed dose of 0, 0.5, 1.0, 3.0, 5.0 kGy using Gamma-ray of cobalt and Electron-beam of linear accelerator. Then, the number of bacteria was measured in the sample by the decimal dilution method. After sterilization, a non-parametric testing method was performed to compare the survival of Escherichia coli and Streptococcus mutans. As a result, Escherichia coli was sterilized at 1 kGy or more and Streptococcus mutans at 3 kGy or more on absorbed dose. It is considered possible to perform sterilization at a lower value than the recommended absorbed dose of radiation sterilization.

• Journal of Biosystems Engineering
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• v.32 no.6
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• pp.408-415
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• 2007

Leaf vegetables are composed of about 80% of moisture and 20 to 30% are damaged during the transportation due to decomposition and browning. This study was conducted to develop a washing, sterilization dehydrating system that could remove these polluters, and major findings were summarized as followings. The system was constructed so that leaf vegetables were moved through the washing, sterilization, and dehydration stages contained in a box, the shifting was conducted using PLC-controlled pneumatic pressure. Basic tests for determination of design parameters led to the results that optimum washing times by air bubbles were 5 minutes for lettuce and perilla and 10 minutes for Chinese cabbage, and percentages of bacteria removed using the optimum washing times were 94, 98, and 76%, respectively. Optimum speed and time of a centrifugal dehydrator for removal surface waterdrop on the leaves were 400 rpm and 30 s, respectively, dehydration rates were in a range $70{\sim}82%$ and damage rates were within 15% for the three vegetables tested. Operating efficiencies of the developed system for lettuce, perilla, and Chinese cabbage were 100, 120, and 80 kg/h, respectively, which were 25, 24, and 27 times of operating efficiencies of the conventional manual method.

• Korean Chemical Engineering Research
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• v.49 no.6
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• pp.835-839
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• 2011

The characteristics of torch-type atmospheric pressure plasma and its sterilization effects have been analyzed. The length of plasma flame was varied with the level of applied voltage and the mixture gases composed of argon and oxygen. The effect of plasma flame on the temperature increase of surface treated was limited to $43^{\circ}C$ as a maximum temperature under exposing time of 10 min. The sterilization for E. coli was strongly affected by the applied voltage, the oxygen ratio in the mixture gas and the treatment time. At a high concentration of ozone, the increase of treatment time under the direct contact with plasma flame yields to maximize the effect of the sterilization on E. coli.

• Journal of Life Science
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• v.9 no.1
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• pp.17-21
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• 1999

Chitosan derivitives, a sulfated N-acetylchitosan was synthesized, and artificial skin of sulfated N-acetylchitosan and N-carboxyl butyl chitosan were investigated. Sulfated derivatives of chitosan were analyzed by {TEX}${13}^C${/TEX}-NMR and the structure on N-acetyl chitosan 3,6-O-disulfate were confirmed. Rabbits underwent a midline laparotomy followed either by a bilateral peritoneal sidewall abraison(3.0×1.5cm). The injured surface was then covered with 0.2mm thick sulfated N-acetyl chitosan membrane. Sulfated N-acetyl chitosan membrane was found to reduce postsurgical bleeding after abraison of peritoneal surface treated with sulfated N-acetyl chitosan membrane. Sulfated N-acetyl chitosan implanted rabbit showed quick wound healing than N-carboxybutyl chitosan. With a sterilization procedure of chemical sterilization, sulfated N-acetyl chitosan seem to be better substitutes than N-carboxybutyl chitosan.

• Korean Journal of Food Science and Technology
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• v.50 no.3
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• pp.331-338
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• 2018

The purpose of this study was to determine the optimum sterilization conditions for the production of retorted steamed egg using response surface methodology. Sterilization processes for eighteen conditions using varying sterilization temperature ($X_1$), time ($X_2$), and method ($X_3$) as the independent variables were carried out through a $3^2{\times}2$ experimental factorial design. Quality evaluations after sterilization included measurements of $F_0$ value ($Y_1$), peak stress ($Y_2$), pH ($Y_3$), color value ($Y_{4-6}$), and organoleptic test [preference for appearance ($Y_7$), overall acceptability ($Y_8$), and preference for texture ($Y_9$) and egg taste ($Y_{10}$)]. Dependent variables ($Y_{1-10}$) of eighteen conditions were more affected by temperature and time than by the sterilization method. Eight factors were selected among the dependent variables as significant factors related to the quality of the steamed egg. Finally, by establishing an optimum range of each dependent variable and contour analysis, the optimum sterilization conditions for the production of steamed egg were determined to be $120^{\circ}C$ for 25 min using a 2-step sterilization process.

• Journal of Biosystems Engineering
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• v.34 no.4
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• pp.234-242
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• 2009

This study was attempted to develop surface washing-system of ginseng. The effect of sterilization, washing and keeping freshness of ginseng through analyzing unit process were examined to establish optimal condition for washing system. Surface washing method of fresh ginseng used two way and full cone spray type. Sterilization was used at $2^{\circ}C$ water with electrolysis water of 50 and 80 ppm. Ginseng was sterilized with electrolysis water during 30 and 60 s, dehydrated during 1 min and dried during 1min at 30 and $50^{\circ}C$. Hardness of surface-washed ginseng showed good result on 1 min spraying time with 80 ppm electrolysis water at $10^{\circ}C$ storage. Ginseng with 80 ppm electrolysis water was sterilized better with $1.05{\times}103$. There are no changes with 0% on appearance quality at 80 ppm electrolysis.