• Title/Summary/Keyword: surface display

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Site-specific Dye-labeling of the Bacterial Cell Surface by Bioconjugation and Self-assembly (바이오접합과 자가결합을 이용한 박테리아 세포막의 위치 특이적 형광 표지)

  • Yang, I Ji;Lim, Sung In
    • Korean Chemical Engineering Research
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    • v.60 no.3
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    • pp.398-406
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    • 2022
  • The outer membrane of Gram-negative bacteria is the outermost layer of cellular environment in which numerous biophysical and biochemical processes are in action sustaining viability. Advances in cell engineering enable modification of bacterial genetic information that subsequently alters membrane physiology to adapt bacteria to specific purposes. Surface display of a functional molecule on the outer membranes is one of strategies that directs host cells to respond to a specific extracellular matter or stimulus. While intracellular expression of a functional peptide or protein fused to a membrane-anchoring motif is commonly practiced for surface display, the method is not readily applicable to exogenous or large proteins inexpressible in bacteria. Chemical conjugation at reactive groups naturally occurring on the membrane might be an alternative, but often compromises fitness due to non-specific modification of essential components. Herein, we demonstrated two distinct approaches that enable site-specific decoration of the outer membrane with a fluorescent agent in Escherichia coli. An unnatural amino acid genetically incorporated in a surface-exposed peptide could act as a chemoselective handle for bioorthogonal dye labeling. A surface-displayed α-helical domain originating from a part of a selected heterodimeric coiled-coil complex could recruit and anchor a green fluorescent protein tagged with a complementary α-helical domain to the membrane surface in a site- and hetero-specific manner. These methods hold a promise as on-demand tools to confer new functionalities on the bacterial membranes.

Dual Backlight Unit Incorporating a Single Light Source Integrated with a Beam Splitting Reflector (광분할 반사경이 집적된 단일 광원 기반의 통합형 듀얼 백라이트 유닛)

  • Park, Chan-Kyu;Lee, Hak-Soon;Lee, Sang-Shin
    • Korean Journal of Optics and Photonics
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    • v.19 no.2
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    • pp.122-126
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    • 2008
  • Dual backlight unit incorporating a single light source integrated with a beam splitting reflector was demonstrated, providing a surface light beam for both the keypad and the display section simultaneously. The reflector was realized by aligning a groove substrate with a matching cover, and a light guiding module comprising a stack of light guide panels and prism/diffuser sheets was attached to both sides of the reflector. A light emanating from the light source-placed in the middle of the substrate-undergoes a series of reflections through the reflector to reach the input of the light guiding module. Then it is transformed into a surface light beam, which is used to irradiate the keypad and display sections simultaneously. As for the accomplished dual surface light sources, the measured average luminance and the spatial luminance uniformity were respectively about $420\;cd/m^2$ and 69% for the keypad section, and $640\;cd/m^2$ and 79% for the display section.

Adhesion Force Measurements of Nano-Imprint Materials Using Atomic Force Microscope (원자력현미경을 이용한 나노임프린트 재료의 접착력 측정)

  • Yun, Hyeong Seuk;Lee, Mongryong;Song, Kigook
    • Polymer(Korea)
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    • v.38 no.3
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    • pp.358-363
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    • 2014
  • Adhesion forces between acrylate imprinting resin and a surface treated atomic force microscope (AFM) tip were investigated. Compared to the untreated silicon tip, 38% of the adhesion force is reduced for the hydrophobic tip treated with $CH_4$ plasma whereas 1.6 time increases is found for the hydrophilic tip with $O_2$ plasma treatment. Such a measurement of the adhesion force using AFM provides very quantitative results on adhesion comparing to the crosscut adhesion test which gives qualitative results. Since the adhesion area becomes larger as the imprinting pattern size gets smaller, the surface treatment issue becomes more important in the nano-imprinting process.

A Study on the Interface and Luminescent Properties of OLED using $Al_2Nq_4$ as an Emitting Layer ($Al_2Nq_4$를 발광층으로 이용한 OLED의 계면 및 발광 특성에 관한 연구)

  • Yang, Ki-Sung;Lee, Ho-Sik;Shin, Hoon-Kyu;Kim, Doo-Seok;Kim, Chung-Kyun;Kwon, Young-Soo
    • Proceedings of the KIEE Conference
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    • 2004.11a
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    • pp.215-219
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    • 2004
  • Metal-chelate derivatives have been investigated intensively as an emitting layer and recognize to have excellent electroluminescence(EL) properties. We synthesized new luminescent material, 1,4-dihydoxy-5,8-naphtaquinone $Aiq_3$ complex($Al_2Nq_4$) and investigated the electrical optical properties. OLED has potential candidates for information display with merits of thickness, low power and high efficiency. Although the OLED show a lot of advantages for information display, it has the limit of inorganic(metal)/ organic interface. In this study, the two methods are used to study the interface of metal/organic in OLED. First, we treated $O_2$ plasma on an ITO thin film by using RIE system, and analyzed the ingredient of ITO thin film according to change of the processing conditions. We used the RDS and the XPS for the ingredient analysis of the surface and bulk. We measured electrical resistivity using Four-Point-Probe and calculated sheet resistance, and ITO surface roughness was measured by using AFM. We fabricated OLED using substrate that was treated optimum ITO surface. Second, we used the buffer layer of CuPc to improve the characteristics of the interface and the hole injection in OLED. The result of the study for electrical and optical properties by using I V L T System(Flat Panel Display Analysis System), we confirmed that the electrical properties and the luminance properties were improved.

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Large-scale Ambient Display Environment for providing Multi Spatial Interaction Interface (멀티 공간 인터랙션 인터페이스 제공을 위한 대규모 앰비언트 디스플레이 환경)

  • Yun, Chang Ok;Park, Jung Pil;Yun, Tae Soo;Lee, Dong Hoon
    • Proceedings of the Korea Contents Association Conference
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    • 2009.05a
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    • pp.30-34
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    • 2009
  • Recently, systems providing the interaction different according to an interval between a user and the display were developed in order to construct the ambient or the ubiquitous computing environment. Therefore, we propose a new type of spatial interaction system; our main goal is to provide the interactive domain in the large-scale ambient display environment. So, we divide into two zones of interaction dependent on the distance from the interaction surface interactive zone and ambient zone. In interactive zone, the users can approach the interaction surface and interact with natural hand-touch. When the users are outside the range of the interactive zone, the display shows only general information. Therefore, this system offers the various interactions and information to users in the ubiquitous ambient environment.

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Tolerance Analysis of Focus-adjustable Head-mounted Displays

  • Choi, Hojong;Ryu, Jae Myung;Kim, Jin Ha
    • Current Optics and Photonics
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    • v.1 no.5
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    • pp.474-490
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    • 2017
  • Since the recent slowdown in the smartphone market, studies for wearable devices are briskly being carried out to find new markets, such as virtual reality devices. In this paper, a head-mounted display (HMD) which provides expanded virtual images before human eyes by enlarging images of a small display was designed, and the tolerance analysis method for a focus-adjustable HMD based on afocal optical systems was studied. There are two types of HMDs: a see-through type that allows the user to view the surroundings, and a see-close type where the user can only view the display screen; the former is used in this study. While designing the system, we allowed a lens within the system to be shifted to adjust its focus from +1 to -4 D (diopters). The yield of the designed systems was calculated by taking the worst-case scenario of a uniform distribution into account. Additionally, a longitudinal aberration was used rather than MTF for the tolerance analysis with respect to system performance. The sensitivity of the designed system was calculated by assigning a certain tolerance, and the focus lens shift was calculated to adjust the image surface variations resulting from the tolerance. The smaller the tolerance, the more expensive the unit price of the products. Very small tolerances may even be impossible to fabricate. Considering this, the appropriate tolerance was assigned; the maximum shift of the focus lens in which the image surface can be adjusted was obtained to find the changes in aberration and a good yield.

The Effect of Call Center Consultant 's Emotional Labor on Burnout: The Moderating Effect of Resilience and Social Support (콜센터 상담원의 감정노동이 소진에 미치는 영향: 회복 탄력성과 사회적 지지의 조절효과)

  • Park, Hayoung;Kim, Jungkyu
    • Stress
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    • v.26 no.4
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    • pp.340-349
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    • 2018
  • Background: The purpose of this study was to examine the moderating effect of resilience and social support to analyze that call center consultants' emotional labor impacts on the burnout. Methods: A survey questionnaire was conducted to people who work at call center in Gwangju and Deagu. The data from 444 were analyzed. Results: The result from the hierarchical multiple regression is as follows. First, deep acting decreased burnout, but surface acting of emotional labor increased burnout. Also, frequency of emotion display, variety of emotion required to be expressed and attentiveness to required display rules increased burnout. Second, Resilience had moderating effect between deep acting and burnout. Third, the moderate effect of interaction of social support from leader with surface action was found at burnout. Also, moderating effect of social support from leader were shown in frequency of emotion display with burnout and attentiveness to required display rules with burnout. Fourth, social support from colleague did not have buffering effect on emotional labor and burnout. Conclusions: It is expected that the moderator variables identified in this study can be used for prevention and treatment of burnout caused by emotional labor.

Application of Nanoparticles for Materials Recognition using Peptide Phage Display Technique- Part I: Preliminary study using LaPO4 and TiO2 nanoparticles (Peptide phage display 기술을 이용한 나노입자의 materials recognition 응용 - Part I: LaPO4 및 TiO2 나노입자를 이용한 기초연구)

  • Lee, Chang-Woo;Kim, Min-Jung;Standaert, R.;Kim, Seyeon;Owens, E.;Yan, Jun;Choa, Yong-Ho;Doktycz, M.;Lee, Jai-Sung
    • Korean Journal of Metals and Materials
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    • v.46 no.1
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    • pp.6-12
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    • 2008
  • Peptides with specific sequences against $LaPO_4$ and $TiO_2$ nanoparticles were discovered through peptide phage display technique as an application to biomolecular recognition of inorganic materials. Sequencing results showed that a motif consisting of serine and proline was commonly expressed in specific sequences. It was postulated that serine directly bound to nanoparticles using its terminal hydroxyl (OH) group. In this sense, oxygen atom seemed to work as a ligand to metal ions and hydrogen atom as a H-bond donor, was thought to bind to the oxygen atoms or the hydroxyl groups on particle surface. Also, it was expected that proline assists serine to make an ideal van der Waals contact between serine and nanoparticles, which optimizes the binding of peptide onto surface.

Effect of various MgO E-beam evaporation sources on the characteristics of MgO protecting layer of AC-PDP

  • Park, Sun-Young;Lee, Mi-Jung;Kim, Soo-Gil;Kim, Hyeong-Joon;Moon, Sung-Hwan;Kim, Jong-Kuk
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.223-226
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    • 2004
  • MgO thin films were deposited bye-beam evaporation on $SiO_2$/Si wafers for the application of a protective layer in alternating current plasma display panels (AC-PDPs). Three different MgO sources, single crystal, melted polycrystal and sintered polycrystal, were used to find out the change of the properties of MgO protective layer depending on the source type. The properties of MgO thin films such as density, orientation and surface morphology were influenced by the source type. MgO thin films deposited with the melted polycrystal source had the highest density with the highest (100) preferred orientation, whereas the films deposited with the sintered polycrystal source had the lowest density with less preferred orientation. Such a result seems to be originated from the different mobility of adatoms on the surface of the deposited MgO thin films. Different microstructures of MgO thin films deposited even in the same deposition condition were observed depending on the MgO source type, resulting in different discharge characteristics.

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