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The Effect of Photomodulation in Human Dermal Fibroblasts (피부 섬유아세포에서 광자극의 효과)

  • Kim, Mi Na;Kwak, Taek Jong;Kang, Nae Gyu;Lee, Sang Hwa;Park, Sun Gyoo;Lee, Cheon Koo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.4
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    • pp.325-331
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    • 2015
  • Skin is exposed to sunlight or artificial indoor light on a daily. The reached solar light on the earth surface consist of 50% visible light and 45% infrared (IR) except for ultra violet (UV). The negative effects of UV including UVB and UVA have been steadily investigated within the last decades. However, little is known about the effects of visible or IR light. In this study, we irradiated human dermal fibroblasts using light emitting diode (LED) to investigate the optimal parameter for enhancing cell growth and collagen synthesis. We found that red of 630 nm and green of 520 nm enhance the cell proliferation, but irradiation with purple and blue light exerts toxic effects. To examine the response of irradiation time and light intensity on the fibroblasts, cells were exposed to red or green light with intensities from 0.05 to $0.75mW/cm^2$. Procollagen secretion was increased of 1.4 fold by 10 min irradiation, while 30 min treatment decreased the collagen synthesis of dermal fibroblasts. Treatment with red of $0.3mW/cm^2$ and green of 0.15 and $0.3mW/cm^2$ resulted in enhancement of collagen mRNA. Lastly, we investigated the combinatorial effect of red and green light on dermal fibroblasts. The sequential irradiation of red and green light is an efficient way for the purpose of the increase in the number of fibroblasts than single light treatment. On the other hand, the exposure of red light alone was more effective method for enhancing of collagen secretion. Our study showed that specific light parameters accelerated cell proliferation, gene expression and collagen secretion on human dermal fibroblasts. In conclusion, we demonstrate that light exposure with specific parameter has beneficial effects on the function of dermal fibroblasts, and suggests the possibility of its cosmetically and clinical application.

Effect of Halophilic Bacterium, Haloarcula vallismortis, Extract on UV-induced Skin Change (호염 미생물(Haloarcula vallismortis) 용해물의 자외선유발 피부변화에 대한 효과)

  • Kim, Ji Hyung;Shin, Jae Young;Hwang, Seung Jin;Kim, Yun Sun;Kim, Yoo Mi;Gil, So Yeon;Jin, Mu Hyun;Lee, Sang Hwa
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.4
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    • pp.341-350
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    • 2015
  • Skin carrys out protective role against harmful outer environment assaults including ultraviolet radiation, heavy metals and oxides. Especially, ultraviolet-B (UVB) light causes inflammatory reactions in skin such as sun burn and erythma and stimulates melanin pigmentation. Furthermore, the influx of UVB into skin cells causes DNA damage in keratinocytes and dermal fibroblasts, inhibition of extracellular matrix (ECM) synthesis which leads to a decrease in elasticity of skin and wrinkle formation. It also damages dermal connective tissue and disrupts the skin barrier function. Prolonged exposure of human skin to UVB light is well known to trigger severe skin lesions such as cell death and carcinogenesis. Haloarcula vallismortis is a halophilic microorganism isolated from the Dead Sea, Its growth characteristics have not been studied in detail yet. It generally grows at salinity more than 10%, but the actual growth salinity usually ranges between 20 to 25%. Because H. vallismortis is found mainly in saltern or salt lakes, there could exist defense mechanisms against strong sunlight. One of them is generation of additional ATP using halorhodopsin which absorbs photons and produces energy by potential difference formed by opening the chloride ion channel. It often shows a color of pink or red because of their high content of carotenoid pigments and it is considered to act as a defense mechanism against intense UV irradiation. In this study, the anti-inflammatory effect of the halophilic microorganism, H. vallismortis, extract was investigated. It was found that H. vallismortis extract had protective effect on DNA damage induced by UV irradiation. These results suggest that the extract of halophilic bacterium, H. vallismortis could be used as a bio-sunscreen or natural sunscreen which ameliorate the harmful effects of UV light with its anti-inflammatory and DNA protective properties.

Application of Potassium Feldspar pIR-IRSL Method to Dating Quaternary Marine and Fluvial Terrace Sediments in Korea: A Case Study on a Fluvial Terrace and Gusan Fault in Uljin, Korea (한반도 해안-하안단구 퇴적층에 대한 K 장석 pIR-IRSL연대측정법 적용가능성 고찰: 울진 하안단구와 구산단층 연대측정)

  • Hong, Seongchan;Choi, Jeong-Heon
    • The Journal of the Petrological Society of Korea
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    • v.25 no.3
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    • pp.241-252
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    • 2016
  • In this paper, in order to test the possibility of applying K feldspar $pIR-IRSL_{290}$ signal(read out at $290^{\circ}C$) to date old terrace sediments(up to ~ 200 ka, MIS 7) in Korea, we investigated luminescence properties of $pIR-IRSL_{290}$ signals in K feldspar extracts from 27 marine and fluvial terrace sediment samples, and these were compared with those of quartz OSL and conventional K feldspar $IRSL_{50}$ (readout at $50^{\circ}C$) signals. The averaged $2D_0$ value of K feldspar $pIR-IRSL_{290}$ growth curves was ~ 700 Gy, which is consistent with that of $IRSL_{50}$ signal, and this is 3 times higher than that for quartz OSL (~ 250 Gy) on average. Where possible, K feldspar $pIR-IRSL_{290}$ ages were compared with quartz OSL and conventional $IRSL_{50}$ ages. Our preliminary K feldspar $pIR-IRSL_{290}$ ages were older than quartz OSL ages by about 200%, while fading rate-corrected conventional $IRSL_{50}$ ages are in good agreement with those based on quartz OSL. This seems to indicate the possibility of K-feldspar $pIR-IRSL_{290}$ age overestimation due to the presence of unbleachable $pIR-IRSL_{290}$ signals, even with a prolonged exposure to sunlight. Both quartz OSL and K-feldspar $pIR-IRSL_{290}$ signals for the samples from Noeum fluvial terrace and Gusan fault site were all in dose saturation level, thus unable to estimate the formation ages of the sediments. However, $2D_0$ values derived from the dose response growth curves strongly indicate that the Noeum fluvial terrace sediments have formed before 109-140 ka, while the fluvial sediments from Gusan fault were desposited before 100-105 ka. Further, this seems to suggest that the previous quartz OSL ages of ~40-50 ka for Gusan fault sediments should be the underestimated ones due to dose saturation problem.

Estimation of Nitrogen Uptake and Biomass of Rice (Oryza sativa L.) Using Ground-based Remote Sensing Techniques (지상 원격측정 센서를 활용한 벼의 생체량과 질소 흡수량 추정)

  • Gong, Hyo-Young;Kang, Seong-Soo;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.779-787
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    • 2011
  • This study was conducted to evaluate the usefulness of ground-based remote sensing for the estimation of rice yield and application rate of N-fertilizer during growing season. Dongjin-1, Korean cultivar of rice was planted on May 30, 2006 and harvested on October 9, 2006. Chlorophyll content and LAI (leaf area index) were measured using Minolta SPAD-502 and AccuPAR model LP-80, respectively. Reflectance indices were determined with passive sensors using sunlight and four types of active sensors using modulated light, respectively. Reflectance indices and growth rate were measured three times from 29 days to 87 days after rice plating and at harvesting day. The result showed that values of growing characteristics and reflectance indices were highly correlated. Growing characteristics to show significant correlation with reflectance indices were in order of followings: fresh weight > N uptake > dry weight > height > No. of tiller > N content. Chlorophyll contents measured by chlorophyll meter (SPAD 502) showed high correlation with nitrogen concentration (r=$0.743^{**}$), although the correlation coefficients between remote sensing data and nitrogen concentration were higher. LAI was highly correlated with dry weight (r=$0.931^{**}$), but relationship between LAI and nitrogen concentration (r=$0.505^*$) was relatively low. The data of CC-passive sensor were negatively correlated with those of the near-infrared. NDVI correlation coefficients found more useful to identify the growth characteristics rather than data from single wavelength. Both passive sensor and active sensor were highly significantly correlated with growth characteristics. Consequently, quantifying the growth characteristics using reflectance indices of ground-based remote sensing could be a useful tool to determine the application rate of N fertilizer non-destructively and in real-time.

Soil Management Techniques for High Quality Cucumber Cultivation in Plastic Film Greenhouse (고품질 시설하우스 오이재배를 위한 토양 종합관리 기술)

  • Hyun, Byung-Keun;Jung, Sug-Jae;Jung, Yeon-Jae;Lee, Ju-Young;Lee, Jae-Kook;Jang, Byoung-Choon;Chio, Nag-Doo
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.717-721
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    • 2011
  • In case of plastic film greenhouses cultivating fresh vegetables on paddy soil, soil characteristics must be considered as more important factor than any other factors. Generally after the four years of cultivation, soils tend to increase electrical conductivity value, nutrient unbalance and soil pests. As a result, degradation of agricultural products occurred, therefore it is necessary to improve soil conditions. In this study, yield and economic cost of cucumber were analyzed. The best soil conditions for cucumber cultivation were alluvial or valley in soil topology, moderately or poorly drainage in soil drainage classes, coarse loamy soil in texture. In addition, rich-sunlight and-deep groundwater would be proper for the cucumber cultivation. Good environmental managements of plastic film greenhouse were as follows. The temperature needed to be adjusted three times. The optimal daytime temperature could be $22{\sim}28^{\circ}C$, the one from 12 until night could be $14{\sim}15^{\circ}C$, and the temperature from 24 to sunrise could be $10{\sim}12^{\circ}C$. During plant growth period, soil moisture content was as low as 10~15%, and it needed to be maintained as 15~20% during reproductive growth period. To control pests, catch crop cultivation and solar treatment were carried out, after those EC was reduced and the root-knot nematode was controled too. Cucumber yield from the plot with improved soil managements increased to $158.4Mg\;ha^{-1}$, but plot with successive cropping injury yielded $140.3Mg\;ha^{-1}$. The income from the plot with improved soil managements was 215,676 thousand won $ha^{-1}$, the plot with successive cropping injury was 131,649 thousand won $ha^{-1}$. Income rate of each plot was 51.8% and 38.4%, respectively.

Effect of the Elevated Carbon Dioxide on the Growth and Physiological Responses of Peach 'Mihong' (CO2 상승처리가 복숭아 '미홍'의 수체생육 및 생리반응에 미치는 영향)

  • Lee, Seul Ki;Cho, Jung Gun;Jeong, Jae Hoon;Ryu, Suhyun;Han, Jeom Hwa;Do, Gyung-Ran
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.312-319
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    • 2021
  • This study was conducted to investigate the effect of elevated carbon dioxide on the growth and physiological responses of peach 'Mihong' (Prunus persica). We simulated three different carbon dioxide conditions based on climate change scenarios RCP 8.5 in the sunlight phytotron rooms from April 22 to July 6, 2020; 400 µmol·mol-1(present condition), 700 µmol·mol-1 treatment(expecting carbon dioxide concentrations in mid-21st century), 940 µmol·mol-1 treatment (expecting carbon dioxide concentrations in late 21st century). The average of maximum photosynthesis rate at 700 µmol·mol-1(16.06 µmol·CO2·m-2·s-1) was higher than those at 400 µmol·mol-1(14.45 µmol·CO2·m-2·s-1) and 940 µmol·mol-1(15.96 µmol·CO2·m-2·s-1) from May 22 to July 2. However, stomatal conductances at 700 µmol·mol-1 and 940 µmol·mol-1 were lower than those at the control. Also, the carbon dioxide saturation point in all treatments was reduced from 1,200 µmol·mol-1 in the early stage of growth to 600-800 µmol·mol-1 in the late stage of growth. The stomatal densities were decreased as carbon dioxide increased. The shoot lengths were decreased while the carbon dioxide was increased, but the increase of trunk diameter and leaf areas, shoot numbers were not statistically different. The fruit weight at 700 µmol·mol-1(152.5 g) was higher than those at the control(141.8 g) and 940 µmol·mol-1(147.4 g). The soluble solids were higher at 700 µmol·mol-1, 940 µmol·mol-1 compared to the control. These results suggest that a carbon dioxide elevated to 700 µmol·mol-1 in the future may give a positive effect on the yield and fruit quality of peach 'Mihong' while a carbon dioxide elevated above 940 µmol·mol-1 may affect negatively such as early senescence and loss of fruit set.

Study on the Current Status of Smart Garden (스마트가든의 인식경향에 관한 연구)

  • Woo, Kyung-Sook;Suh, Joo-Hwan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.2
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    • pp.51-60
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    • 2021
  • Modern society is becoming more informed and intelligent with the development of digital technology, in which humans, objects, and networks relate with each other. In accordance with the changing times, a garden system has emerged that makes it easy to supply the ideal temperature, humidity, sunlight, and moisture conditions to grow plants. Therefore, this study attempted to grasp the concept, perception, and trends of smart gardens, a recent concept. To achieve the purpose of this study, previous studies and text mining were used, and the results are as follows. First, the core characteristics of smart gardens are new gardens in which IoT technology and gardening techniques are fused in indoor and outdoor spaces due to technological developments and changes in people's lifestyles. As technology advances and the importance of the environment increases, smart gardens are becoming a reality due to the need for living spaces where humans and nature can co-exist. With the advent of smart gardens, it will be possible to contribute to gardens' vitalization to deal with changes in garden-related industries and people's lifestyles. Second, in current research related to smart gardens and users' experiences, the technical aspects of smart gardens are the most interesting. People value smart garden functions and technical aspects that enable a safe, comfortable, and convenient life, and subjective uses are emerging depending on individual tastes and the comfort with digital devices. Third, looking at the usage behavior of smart gardens, they are mainly used in indoor spaces, with edible plants are being grown. Due to the growing importance of the environment and concerns about climate change and a possible food crisis, the tendency is to prefer the cultivation of plants related to food, but the expansion of garden functions can satisfying users' needs with various technologies that allow for the growing of flowers. In addition, as users feel the shapes of smart gardens are new and sophisticated, it can be seen that design is an essential factor that helps to satisfy users. Currently, smart gardens are developing in terms of technology. However, the main components of the smart garden are the combination of humans, nature, and technology rather than focusing on growing plants conveniently by simply connecting potted plants and smart devices. It strengthens connectivity with various city services and smart homes. Smart gardens interact with the landscape of the architect's ideas rather than reproducing nature through science and technology. Therefore, it is necessary to have a design that considers the functions of the garden and the needs of users. In addition, by providing citizens indoor and urban parks and public facilities, it is possible to share the functions of communication and gardening among generations targeting those who do not enjoy 'smart' services due to age and bridge the digital device and information gap. Smart gardens have potential as a new landscaping space.

The Method and Meaning of the Archiving Project of Suicide Survivors (자살유족 기록작업의 방법과 의미)

  • Lee, Young-nam
    • The Korean Journal of Archival Studies
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    • no.59
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    • pp.207-275
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    • 2019
  • This archiving project of the survivors of suicide was done with the survivor supporting team of the Seoul Suicide Prevention Center. The survivor supporting team was operating a Self-help Support Group for the emotional support of the survivors of suicide. A Self-help Support Group is a place for the survivors of suicide to regularly meet and share their suffering by talking of topics hard to discuss elsewhere. As the Self-help Support Group progressed members who acted as the leader of the group appeared. They formed an essay group that writes together. Two fathers who lost their sons, two mothers who lost their daughters, a mother who lost her son, a wife who lost his husband. The essay group met each week in a place facing Sajik Park. Through the windows that took up the whole side of the room, evening was coming in. The things that happened during the day went away towards Inwang mountain following the setting sun. Ten people (six members of the essay group, three from the survivor support team, a historian for unique conversation) sat around a table, facing each other. "Now, what shall we do?" History for unique conversation is a time that archives life by sharing conversations. At times a complete stranger, and other times people who share their ordinary lives sit around together (3-9 people, sometimes about 15). On the table there is coffee, bread, fruits and salads, and sometimes a dish someone heartily prepared. When a bottle of wine is placed on the table, each takes a glass. Morning, afternoon, the time the evening is welcomed in, late night. It does not matter which. For six months, 3 hours when meeting every week, 6 hours when at every other week. A room where the ambience is like that of a kitchen where sunlight enters, or a cozy living room is the best location. However, there are many times when it is held in a multipurpose room in the suburbs where many meetings are held, or in a classroom of a school. The meeting place is decided according to different situations of the time. There are no participation requirements as it is said to be for themselves to write down according to archiving form while looking back their lives thoroughly, and they are the only ones to stop themselves. The archives landscape from far away would seem like trying to do some talking. However, when going into a microscopic situation one must leave themselves to the emotional dynamics. It is because it archives the frustration and failures one experienced through life. A participator of history for unique conversation must face the sufferings of their life. The archiving project took place in 2013 to 2014. Many years have passed. Has the objective distance for archiving the situation of that time been secured? That may be uncertain, but I will speak of a few stray thoughts on archiving while depicting the process and method of operation.

A Study on the Problems of Eating Habits of Mordern People and Suggesting Alternatives to Overcome Diseases: A Review of the Five Blue Zones, Based on the Roma Linda Region in the USA (현대인의 식습관 문제점 인지와 발생 질병극복을 위한 대안 제시: 5대 블루존 중 미국 로마린다 지역을 중심으로)

  • Shin, Kyung-Ok;Je, Haejong
    • Journal of Korean Society of Neurocognitive Rehabilitation
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    • v.10 no.2
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    • pp.53-62
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    • 2018
  • The purpose of this study was to propose an alternative for the eating habits of modern people and coping with the diseases. The purpose of this study was to apply the principles of eating habits of people living in Roma Linda to modern dietary life and to help healthy life and prevent disease. The period of this study was from May 1, 2016 to February 28, 2018. Literature search was conducted using Pubmed and Korean academic web sites. Based on the recognition of wrong eating habits, we classify and classify diseases according to eating habits. A total of more than 100 papers were selected and 60 papers and a database were prepared. People living in Roma Linda have eight health principles. The Roma Linda practiced balanced nutritional intake, sufficient exercise, adequate water intake, sunlight, temperance (abstinence from alcohol etc.), fresh air, adequate rest, and trust in eating habits. People living in Roma Linda have a high intake of vegetables, fruits and nuts. People living in Roma Linda are educated about nutrition, and among them, there is a low prevalence of coronary heart disease and cancer, because they mostly do not smoke or drink alcohol. Unhealthy eating habits and dietary behavior are associated with many diseases. Many chronic, degenerative diseases are due to bad eating habits and stress. If you take good food habits of people living in the Roma Linda area and practice it steadily, it will have a great effect on disease prevention.

Evaluation of the Growth and Yield of Sweetpotato (Ipomoea batatas L.) at Different Growth Stages under Low Light Intensity (생육시기별 차광 처리에 의한 고구마 생육 및 수량성 평가)

  • Park, Won;Chung, Mi Nam;Nam, Sang-Sik;Kim, Tae Hwa;Lee, Hyeong-Un;Goh, San;Lee, Im Been;Shin, Woon-Cheol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.2
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    • pp.146-154
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    • 2021
  • This study was conducted to determine the degree of reduction in the yield of sweetpotato subjected to different shading treatments according to the growing season of the typical viscous sweetpotato 'Hogammi' and the powdery sweetpotato 'Jinyulmi'. Shading was provided using commercially available shading nets (55% and 75% shading level), and the treatments were applied at the following stages of storage root growth: SFS: the storage root formation stage (planting-50th day), SSS: the storage root swelling stage (50-90th day), and SAS: the storage root actively swelling Stage (90-120th day). The growth characteristics according to shading treatments during each growth period, the number of tubers obtained at harvest, and sugar contents were investigated. For both assessed cultivars, there was no significant difference between the control group and the 55% shading treated group with respect to the maximum quantum yield (Fv/Fm) of photosystem II under different shading treatments, whereas the 75% shading group showed slightly higher values than the control group. In both cultivars, the contents of chlorophyll a and b tended to increase in plants subjected to shading treatments compared with the control plants, particularly that of chlorophyll b. Compared with the control group, the chlorophyll b content of 'Hogammi' subjected to 55% and 75% shading increased by 47% and 41%, respectively, whereas that of 'Jinyulmi' increased by 39% and 34%, respectively. We also detected reductions in the dry weights of the above- and belowground parts of the two varieties in response to shading compared with the control, with the reduction in the dry weight of belowground parts being significant. Furthermore, in both varieties, the T/R rate tended to increase in response to shading treatment. Owing to the lack of sunlight, both cultivars tended to suppress the formation and enlargement of tuber roots. Consequently, post-harvest yield analysis revealed that under shading treatments, both cultivars were characterized by poor tuber root growth according to growing season, with the yield of 'Hogammi' showing a greater reduction compared with that of 'Jinyulmi'. In addition, we found that the higher shading level also significantly reduced yields. Compared with the storage root formation and storage root actively swelling stages, shading treatments during the storage root swelling stage significantly affected yield reduction in both varieties.