• Journal of the Korean Chemical Society
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• v.8 no.2
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• pp.47-56
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• 1964

When a developed paper partition chromatogram was irradiated by means of the pneumatic tube system of the Korean research reactor (neutron flux: 1.5 ${\times}10^{12}n/cm^2$sec.) the qualitative confirmation of the developed spot on the chromatogram was possible. In the case of an organic halogen compounds (chloro-acid, chloro-ester, iodide, and fluoride) the spot analysis was possible by the present method whereas the same spot could not give the distinct coloring with a common coloring reagents. Filterpaper thickness calibration and activity calibration induced by irradiation of the components of the filter paper, which were a source of erraneous interpretation of the spot, were searched and an average filterpaper calibration method and filter paper activity were improvised to obtain a good qualitative analysis of the spot. Finally the use and applicability of this method for the analysis identification of an organic halogen compound were evaluated. As the filter paper phase an ordinary phase (Whatmann #1, filter paper) and reversed phase (liquid paraffin impregnated) were used.

• Journal of Astronomy and Space Sciences
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• v.24 no.2
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• pp.91-98
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• 2007

The existence of the North-South asymmetry of the solar activity is widely accepted even though the phenomenon is not yet satisfactorily understood. We have studied the temporal variations in the North-South asymmetry of the sunspot area during the period from May in 1874 to April in 2007. The combined data have been examined for periodicity in the solar activity North-South asymmetry. We have found that (i) solar northern and southern hemispheres show an asymmetric behavior, in terms of a sunspot area, (ii) sunspot areas in northern and southern hemispheres show clearly the 11 year periodicity when they were analyzed separately, as they do when analyzed together, (iii) in addition to the general 11 year periodicity in both northern and southern hemisphere solar activities, there are also noticeable shorter periodicities other than the 11 year periodicity in the asymmetry in North-South activity. Finally, we conclude by pointing out the importance of studying the North-South asymmetry of the solar activity.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2011.06a
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• pp.27-28
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• 2011

A solar flares have the 11-year cycle and release a large energy which may produce coronal mass ejections (CME). The NOAA (National Oceanic and Atmospheric Administration) predicted that the sun spot activity will be maximized in 2013-2014. A strong solar flare can cause the disturbance of global positioning system including various communication of TV, radio broadcasting. The actual solar storm in 1989 caused power outages in Canada during 9 hours and about 600 million people had experienced a blackout. Such a solar storm can shorten the GPS satellite's life span about 5 to 10 years which can be resulted in economic loss considering the amount of multi-billion won. This paper analyzed the recent solar storm of X-class occurred on 15th of February about 10:45 this year that was reached Korea (Bohyun observatory) on 18th of February about 10:30 (local time), and compared with the data of before and after a week. The proton data of 18th of February considered that the solar strom reached on earth showed a fluctuation compared to the data of before and after a week. The positioning results at Daejeon also showed higher positioning error compared to the data of before and after a week results.

• Journal of Navigation and Port Research
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• v.35 no.6
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• pp.477-482
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• 2011

Solar flares have the 11-year cycle and release a large energy which may produce coronal mass ejections (CME). The NOAA (National Oceanic and Atmospheric Administration) predicted that the sun spot activity will be maximized in 2013-2014. A strong solar flare can cause the disturbance of global positioning system including various communication of TV, radio broadcasting. The actual solar storm in 1989 caused power outages in Canada during 9 hours and about 600 million people had experienced a blackout. Such a solar storm can shorten the GPS satellite's life span about 5 to 10 years which can resulted in economic loss considering the amount of multi-billion won. This paper analyzed the influence of recent X-class solar storm occurred on 15th of February about 10:45 this year that was reached Korea (Bohyun observatory) on 18th of February about 10:30 (01:30 - UTC), and compared with the data before and after a week. The proton data of 18th of February considered that the solar storm reached on earth showed a fluctuation compared to the data before and after a week. The positioning results at Daejeon and Seoul of Korea also showed higher positioning error compared to the data before and after a week results.

• Journal of Korean Society of Rural Planning
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• v.14 no.3
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• pp.75-87
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• 2008

Changes in society organization of rural villages in our country are caused by aging society and lessening of economic activity population aggravates the economy of rural villages. With this, it joins in with the aging of village community center, the representative center space of the rural village and therefore, it is a point of view where the change is necessary in the size and the side of function. The research which it sees is made on-the-spot inspection and literature investigation compensate in behalf of 25 administrative village community centers led in the object by grasping present status and drew up the layout sketches and plane surface degrees of the village community centers. By referring to the photographs and the topographical maps taken in the spot, it was able to divide the village community centers into side of arrangement, side of plane surface and side of form and managed to seize the characters, problems and improvable directions of each of them. The research result could be used as a guidable provision and be applied in the fundamental research in developing model of village community centers during space planning in the future.

• Food Science of Animal Resources
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• v.31 no.3
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• pp.405-412
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• 2011

The purpose of this study was to isolate and characterize bacteriocin-producing bacteria against Clostridium perfringens from domestic animals to determine their usefulness as probiotics. The feces of cattle and chicken were used as sources to isolate bacteriocin-producing bacteria using the spot-on-lawn method. In total, 900 bacterial stains were isolated from domestic animal feces, and 19 strains were finally selected after determining the inhibitory activity against the pathogenic indicator C. perfringens KCTC 3269. Eighteen strains of Bacillus subtilis and one strain of Brevibacillus parabrevis were identified by 16s rRNA sequencing. Most of the bacterial strains isolated were resistant to 0.5% bile salts and remained viable after 2 h at pH 3.0. Additionally, some B. subtilis strains showed strong inhibitory activity against Listeria monocytogenes. We isolated and screened B. subtilis strains CB 153 and CB 189 from cattle and B. subtilis MSC 156 and B. parabrevis MSC 164 from chickens using probiotic selection criteria such as inhibition activity against C. perfringens and tolerance to acid and bile salts. The isolated bacteriocin-producing bacteria and/or bacteriocin have the potential to be used as probiotics in the livestock industry.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.879-886
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• 2014

This study was conducted to test in vitro the antagonistic effect of composted liquid manure (CLM) against soil-borne turfgrass pathogenic fungi, Rhizoctonia solani AG-2-2 (IIIB) (brown patch), R. solani AG-2-2 (IV) (large patch), and Sclerotinia homoeocarpa (dollar spot) for environmentally friendly turfgrass management. CLMs were collected from 9 livestock excretion treatment facilities around the country including Gunwi (GW), Hapcheon (HC), Hoengseong (HS), Icheon (IC), Iksan (IS), Muan (MA), Nonsan (NS), and Yeoju (YJ). CLMs of IC, GW, and IS showed s ignificant (p < 0.05) mycelium growth inhibition that was 17.8%, 20.4%, and 48.0% against R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. A t otal of 110 bacterial isolates were obtained from the CLMs that showed antagonistic effects. Among them, 5, 4, and 10 microbe isolates showed promising antifungal activity against mycelium growth of R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. The bacterial isolates ICIIIB60, GWIV70, and ISSH20 effectively inhibited the mycelial growth of three soil-borne turfgrass pathogens. Selected bacterial isolates were identified as Alcaligenes sp., Bacillus licheniformis Ab2, and B. subtilis C7-3 through 16s rDNA gene sequence analysis. Among 5 fungicides, the most compatible fungicide with ICIIIB60, GWIV70, and ISSH20 was tebuconazol, toclofos-methyl and toclofos-methyl, respectively. These findings suggested that CLMs could be effectively used not only as organic liquid fertilizer sources but also as biological control agents for soil-borne turfgrass diseases such as brown patch, large patch, and dollar spot.

• Food Science of Animal Resources
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• v.34 no.5
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• pp.614-621
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• 2014

This study was conducted to isolate and characterize bacteriocin-producing bacteria against Clostridium perfringens (C. perfringens) from domestic animals to determine their usefulness as probiotics. Bacteriocin-producing bacteria were isolated from pig feces by the spot-on-lawn method. A total of 1,370 bacterial stains were isolated, and six were tentatively selected after identifying the inhibitory activity against the pathogenic indicator C. perfringens KCTC 3269 and KCTC 5100. The selected strains were identified as Enterococcus faecalis (E. faecalis) by 16s rRNA sequencing. Most of the isolated bacterial strains were resistant to 0.5% bile salts for 48 h and remained viable after 2 h at pH 3.0. Some E. faecalis also showed strong inhibitory activity against Listeria monocytogenes KCTC 3569, KCTC 3586 and KCTC 3710. In the present study, we finally selected E. faecalis AP 216 and AP 45 strain based on probiotic selection criteria such as antimicrobial activity against C. perfringens and tolerance to acid and bile salts. The bacteriocins of E. faecalis AP 216 and AP 45 strains were highly thermostable, showing anticlostridial activities even after incubation at $121^{\circ}C$ for 15 min. These bacteriocin-producing bacteria and/or bacteriocins could be used in feed manufacturing as probiotics as an alternative to antibiotics in the livestock industry.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.132-132
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• 2017

Plants, including Platycodon grandiflorum have been used globally across varied cultures as a safe natural source of medicines. From time immemorial, humans have relied on plants that could meet their basic necessities such as food, shelter, fuel and health. This study was executed to profile proteins from the hormone induced diploid and tetraploid roots using high throughput proteome approach. Two dimensional gels stained with CBB, a total of 64 differential expressed proteins were identified from the diploid root using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 20 differential expressed protein spots ( ${\geq}1.5-fold$) were analyzed using LTQ-FTICR MS whereas a total of 13 protein spots were up regulated and 7 protein spots were down-regulated. However, in the case of tetraploid root, a total of 78 differential expressed proteins were identified from tetraploid root of which a total of 28 differential expressed protein spots (${\geq}1.5-fold$) were analyzed by mass spectrometry whereas a total of 16 protein spots were up regulated and a total of 12 protein spots were down-regulated. However, proteins identified using iProClass databases revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding, oxidoreductase activity, transporter activity and isomers activity. The exclusive protein profile may provide insight clues for better understanding the characteristics of protein function and its metabolic activity that can help for the development of the nutritional and breeding aspects of this economically important medicinal plant.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.157-164
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• 2017

Functional microorganisms decompose various organic matter by enzyme activity and suppress plant disease caused by pathogen. This study was conducted to isolate and select functional microorganisms with protein or carbohydrate degradation activities and antagonistic activity against turfgrass fungal pathogens for eco-friendly turfgrass management in golf course from compost containing livestock manure of poultry or swine. Totally 68 isolates collected from livestock manure compost strains were isolated and tested for their activities of amylase, protease and lipase and antagonistic activities against Rhizoctonia solani AG2-2, R. solani AG1-1, and Sclerotinia homoeocarpa. Among the isolates, 34 strains were selected as functional microbes showing higher activities of amylase and protease. Three isolates of ASC-14, ASC-18, and ASC-35 among the 34 strains were selected as antifungal bacterial strains repressing the above 3 turfgrass fungal pathogens. Analysis results of 16s rRNA gene sequence and phylogenic cluster indicated that ASC-14 and ASC-18 belonged to Bacillus amyloliquefaciens, while ASC-35 was B. subtilis, respectively.