Browse > Article
http://dx.doi.org/10.5660/WTS.2017.6.2.157

Isolation and Selection of Functional Microbes for Eco-friendly Turfgrass Management in Golf Course from Livestock Manure Compost  

Jeong, Je-Yong (Hyosung O&B Co. Ltd.)
Kim, Young-Sun (Hyosung O&B Co. Ltd.)
Cho, Sung-Hyun (Hyosung O&B Co. Ltd.)
Lee, Geung-Joo (Department of Horticultural Science, Chungnam National University)
Publication Information
Weed & Turfgrass Science / v.6, no.2, 2017 , pp. 157-164 More about this Journal
Abstract
Functional microorganisms decompose various organic matter by enzyme activity and suppress plant disease caused by pathogen. This study was conducted to isolate and select functional microorganisms with protein or carbohydrate degradation activities and antagonistic activity against turfgrass fungal pathogens for eco-friendly turfgrass management in golf course from compost containing livestock manure of poultry or swine. Totally 68 isolates collected from livestock manure compost strains were isolated and tested for their activities of amylase, protease and lipase and antagonistic activities against Rhizoctonia solani AG2-2, R. solani AG1-1, and Sclerotinia homoeocarpa. Among the isolates, 34 strains were selected as functional microbes showing higher activities of amylase and protease. Three isolates of ASC-14, ASC-18, and ASC-35 among the 34 strains were selected as antifungal bacterial strains repressing the above 3 turfgrass fungal pathogens. Analysis results of 16s rRNA gene sequence and phylogenic cluster indicated that ASC-14 and ASC-18 belonged to Bacillus amyloliquefaciens, while ASC-35 was B. subtilis, respectively.
Keywords
Amylase and protease activity; Antifungal bacterial strain; Functional microbe; Livestock manure;
Citations & Related Records
Times Cited By KSCI : 18  (Citation Analysis)
연도 인용수 순위
1 Ahn, I.P., Park, K.S. and Kim, C.H. 2002. Rhizobacteria induced resistance perturbs viral disease progress and triggers defenserelated gene expression. Mol. Cells 13(2):302-308.
2 Borriss, R. 2011. Use of plant-associated Bacillus strains as biofertilizers and biocontrol agents, In Bacteria in agrobiology: plant growth response. Maheshwari D. K, Eds; Springer Heidelberg, Germany, pp. 41-76.
3 Borriss, R., Chen, X.H., Ruckert, C., Blom, J., Becker, A., et al. 2011. Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int. J. Syst. Evol. Microbiol. 61:1786-1801.   DOI
4 Chen, H., Xiang, X., Wu, J. W., Zheng, Z. and Yu, Z. 2008. Antagonistic effects of volatiles generated by Bacillus subtilis on spore germination and hyphal growth of the plant pathogen, Botrytis cinerea. Biotechnol. Letters 30(5):919-923.   DOI
5 Chung, H.K., Ryu, J.H., Lee, H.S., Park, M.S., Madhaiyan, M., et al. 2004. Effect of immobilized cells of Pantoea agglomerans on growth promotion of rice (Oryza sativa L.) in the presence of rock phosphates. Kor. J. Soil Sci. Fert. 37(1):41-45.
6 Couch, H.B. 1985. Common names for turfgrass disease. In Common names for plant disease. Plant Dis. Rep. 69:672-675.
7 Ham, S.K., Lim, J.Y. and Lee, Y.M. 2014. The effect of thatch decomposing by application with composted liquid manure and microorganism medium in golf course soil. Weed Turf. Sci. 3(4):342-346. (In Korean)   DOI
8 Jeun, Y.C., Park, K.S. and Kim, C.H. 2001. Different mechanisms of induced systemic resistance and systemic acquired resistance against Colletotrichum orbiculare on the leaves of cucumber plants. Mycobiology 29(1):19-26.   DOI
9 Han, H.S. and Lee, K.D. 2012. Changes of biological and chemical properties during composting of livestock manure with isolated native microbe. Kor. J. Soil Fert. Sci. 45(6):1126-1135. (In Korean)   DOI
10 Han, J.H., Park, K.S., Lee, S.Y. and Kim, J.J. 2012. Effects of Streptomyces spp. on growth of plants and antifungal activity of plant pathogens. Kor. J. Pestic. Sci. 16(4):383-386. (In Korean)   DOI
11 Joa, J.H., Lim, H.C., Han, S.G., Chun, S.J. and Suh, J.S. 2007. Characteristics of Bacillus sphaericus PSB-13 as phosphate solubilizing bacterium isolated from citrus orchard soil. Kor. J. Soil Sci. Fert. 40(5):405-411. (In Korean)
12 Jung, H.K., Kim, J.R., Woo, S.M. and Kim, S.D. 2007. Selection of auxin, siderophore, and cellulose-producing PGPR, Bacillus licheniformis K11 and its plant growth promoting mechanisms. J. Korean Soc. Appl. Biol. Chem. 50(1):23-28. (In Korean)
13 Kim, Y.S., Ham, S.K., Kim, T.S. and Jeong, H.S. 2008. Effect of liquid fertilizer containing medium of Lactobacillus confusa and Pichia anomala on growth in creeping bentgrass. Kor. Turfgrass Sci. 22(2):185-196. (In Korean)
14 Jung, W.C., Shin, T.S., Kim, B.S., Im J.S., Lee, J.H., et al. 2008. Efficacy of antagonistic bacteria for biological control of Rhizoctonia blight (Large patch) on Zoysiagrass. Res. Plant Dis. 14(1):43-50. (In Korean)   DOI
15 Kim, J.N., Kim, I.S., Geon, M.G. and Park, D.H. 2006. Control activities of natural compound, TGR-N1, against turfgrass fungal diseases in vitro and in field. Kor. Turfgrass Sci. 20(1):57-63. (In Korean)
16 Kim, T.S., Ko, M.J., Lee, S.W., Han, J.H., Park, K.S., et al. 2011. Antifungal and proteolytic activity and auxin formation of bacterial strains isolated from highland forest soils of Halla mountain. Kor. J. Pestic. Sci. 15(4):495-501. (In Korean)
17 Kim, Y.S., Lee, C.E., Ham, S.K. and Lee, G.J. 2016. Growth of creeping bentgrass by application of compound fertilizer containing microbes. Weed Turf. Sci. 5(1):42-50. (In Korean)   DOI
18 Kim, Y.S., Ham, S.K. and Lee, S.J. 2010. Effect of liquid fertilizer contained medium of Lactobacillus sp. And Saccharomyces sp. on growth of creeping bentgrass. Kor. Turfgrass Sci. 24(2):138-144. (In Korean)
19 Kim, Y.S., Ma, K.Y. and Lee, G.J. 2015. Antagonistic mechanism and culture conditions of isolated microbes applied for controlling large patch disease in zoysiagrass. Kor. J. Hort. Sci. Technol. 33(4):492-500. (In Korean)
20 Kwon, J.S., Weon, H.Y., Suh, J.S., Kim, W.G., Jang, K.Y., et al. 2007. Plant growth promoting effect and antifungal activity of Bacillus subtilis S37-2. Korean J. Soil Sci. Fert. 40(6):447-453. (In Korean)
21 Kwon, H.D. and Song, H.G. 2014. Interactions between indole-3- acetic acid producing Acinetobacter sp. SW5 and growth of tomato plant. Kor. J. Microbiol. 50(4):302-307.   DOI
22 Lee, S.Y., Lee, Y.K., Park, K.S. and Kim, Y.K. 2010. Selection of beneficial microbial agents for control of fungal diseases in the phyllosphere of cucumber plant. Kor. J. Pestic. Sci. 14(4):326-331. (In Korean)
23 Lee, H.J., Cho, J.S., Bahn, K.N., Heo, J.S. and Shin, W.K. 1998. Changes of the physic-chemical and microbiological properties during composting for composting of sewage sludge. Kor. J. Envrion. Agric. 17(1):16-21. (In Korean)
24 Lee, H.W., Ahn, J.H., Weon, H.Y., Song, J.K. and Kim, B.K. 2013. Diversity and antimicrobial activity of actinomycetes isolated from rhizosphere of rice (Oryza sativa L.). Kor. J. Pestic. Sci. 17(4):371-378. (In Korean)   DOI
25 Lee, J.J., Kim, Y.S., Ham, S.K., Lee, C.E. and Lee, G.J. 2015. Growth and quality improvement of creeping bentgrass by two fertilizers containing Trichoderma species. Weed Turf. Sci. 4(3):249-255. (In Korean)   DOI
26 Lee, S.B., Kim, J.G., Lee, D.B., Lee, K.B., Han, S.S., et al. 2002. Changes of physic-chemical properties and microflora of pig manure due to composting with turning times and depth. Kor. J. Soil Fert. Sci. 35(2):127-135. (In Korean)
27 Lee, S.Y., Kim, B.Y., Ahn, J.H., Song, J.Y., Seol, Y.J., et al. 2012. Draft Genome Sequence of the Biocontrol Bacterium Bacillus amyloliquefaciens Strain M27. J. of Bacteriology. 194:6934-6935. (In Korean)   DOI
28 Lee, S.Y., Weon, H.Y., Kim, J.J. and Han, J.H. 2013. Cultural characteristics and mechanism of Bacillus amyloliquefaciens subsp. palatarum CC110 for biological control of cucumber downy mildew. Kor. J. Pestic. Sci. 17(4):428-434. (In Korean)   DOI
29 Lee, Y.H. and Park, S.R. 2001. Isolation and characteristics of composting-promoting-bacteria. Kor. J. Soil Sci. Fert. 34(6):394-400. (In Korean)
30 Lee, Y.S., Kang, J.H., Choi, K.J., Lee, S.T., Kim, E.S., et al. 2011. Response of soil microbial communities to different cultivation systems in controlled horticultural land. Kor. J. Soil Sci. Fert. 44(1):118-126. (In Korean)   DOI
31 Whang, K.S. and Chang, G.W. 1996. Change of microflora in livestock manure during composting process. Kor. J. Soil Fert. Sci. 29(3):303-311. (In Korean)
32 Lim, H.J., Kim, Y.S. and Ham, S.K. 2011. Screening of cellulose decomposing microorganisms for functional improvement for SCB (slurry composting and biofiltering) liquid fertilizer. Asian J. Turfgrass Sci. 25(1):48-51. (In Korean)
33 Ma, K.Y., Kwark, S.N. and Lee, G.J. 2013. Isolation and selection of antagonistic microbes for biological control of zoysiagrass large patch disease. Kor. J. Hort. Sci. Technol. 31(6):657-665. (In Korean)
34 Park, J.H., Kang, S.Y. and Kim, H.K. 1998. Physiology of Rhizoctonia solani AG2-2(IV), Trichoderma harzianum, and Chaetomium cochliodes, and their utilization of thatch related carbohydrate in Zoysia japonica. Weed Turf. Sci. 12(4):211-220. (In Korean)
35 Park, K.S., Ahn, I.P. and Kim, C.H. 2001. Systemic resistance and expression of the pathogenesis-related genes mediated by the plant growth-promoting rhizobacterium Bacillus amyloliquefaciens EXTN-1 against anthracnose disease in cucumber. Mycobiology 29(1):48-53.   DOI
36 Shrestha, A., Choi, K.U., Lim, C.K., Hur, J.H. and Cho, S. 2009. Antagonistic effect of Lactobacillus sp. strain KLF01 against plant pathogenic bacteria Ralstonia solanacearum. Kor. J. Pestic. Sci. 13(1):45-53.