• Title/Summary/Keyword: sugar utilization

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Purification and Utilization of Industrial Waste Water Using Microorganism -(Part 1) Isolation of the yeast strain from organic waste water and its use on waste water treatment- (산업폐수의 처리 및 이용에 관한 연구 -(제 1 보) 효모균주의 분리와 이에 의한 유기성폐수의 처리에 관하여-)

  • Lee, Kang-Heup;Yim, Sung-Sam;Park, Tai-Won
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.228-235
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    • 1977
  • The yeast strain was isolated from food industry waste water and its identification and biological characteristics were investigated. The optimum condition for cultivations and its activities for the reduction of B.O.D. on the food industry waste water were also confirmed. The results are as follows; 1) The isolated was identified as Candida curvata. 2) Candida curvata grew well in all of the experimented media, so and it can be regarded as a useful strain in the treatment of food industry waste water. 3) There was only a slight difference in the induction period between sterilized cultivation and unsterilized cultivation. But in the ice cream waste water, the period was considerably longer in unsterilized cultivation. 4) Specific rate of growth of Candida curvata in sugar waste water was 0.50/hr, ice cream waste water 0.50/hr, and beer waste water 1.0/hr. 5) Increasing of innoculum reduced the induction period in unsterilized cultivation. 6) The amount of dried yeast from sugar waste water were $175mg/{\ell}$, ice cream waste water $628mg/{\ell}$, and beer waste water $857mg/{\ell}$. Crude protein content in the dried yeast from sugar waste water were 52%, ice cream waste water 54%, and beer waste water 54%. 7) The rate of BOD reduction in sugar waste water were 49%, ice cream waste water 80%, and beer waste water 64%.

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Ethanol Production by Synchronous Saccharification and Fermentation using Food Wastes (음식물 쓰레기 동시당화 발효에 의한 에탄올 생산)

  • Han, Hyo-Jung;Li, Hong-xian;Kim, Seong-Jun
    • KSBB Journal
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    • v.21 no.6 s.101
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    • pp.474-478
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    • 2006
  • For the economically feasible production of ethanol, utilization of SFW (saccharified food wastes) as substrate for synchronous saccharification and fermentation (SSF) process was developed in this study. When 200 g of food wastes and 40 mL of enzyme ($amylase activity,\;3.0\;U/m{\ell}$) were reacted, production rate of reducing sugar was $5.84\;g/{\ell}{\cdot}h$, and consumption rate was $-3.88\;g/{\ell}{\cdot}h\;at\;35^{\circ}C$ So suitable condition of SSF was concluded at temperature of $35^{\circ}C$. Also, optimal enzyme concentration of SSF was concluded in $2.0\;U/m{\ell}$, at this condition, the production rate of reducing sugar was $4.80\;g/{\ell}{\cdot}h$ At SSF process, when 50 g of food wastes was supplied in 12 h interval, $64\;g/{\ell}$ of ethanol and 0.45 g-ethanol/g-reducing sugar in yield were obtained in 120 h fermentation. Thus, the technology of high yield of ethanol production using food wastes was confirmed. And semi-continuos SSF system for cutting off cost of enzymatic saccharification was developed in this study.

Proximate, Sugar and Amino Acid Compositions of Dolsan Leaf Mustard(Brassica juncea) (돌산 갓의 일반성분, 당 및 아미노산 조성)

  • 조영숙;박석규;전순실;문주석;하봉석
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.1
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    • pp.48-52
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    • 1993
  • To furnish basic data for the utilization of leaf mustard as a raw material of salted and fermented vegetable food, major chemical compositions of Dolsan Leaf Mustard (DLM) were investigated. The moisture and ash contents of DLM were 87.5% and 1.4%, respectively. Compared with other Korean traditional or Japanese leaf mustard, DIM contained more crude protein (3.8%) but less crude fat (0.3%) and crude fiber (1.3%). As an alkali food, leaf and leat stalk had pH of 5.7 and 5.8, titratable acidity of 687 and 318mg/100g and alkalinity of 2.5 and 5.2, respectively. The major free sugar in DLM was glucose and fructose was also detected in a small amount. The contents of total and reducing sugar in leaf and leaf stalk were 574, 352, 820 and 538mg%, respectively. Total amino acid contents of leaf and leaf stalk were 8.0 and 2.5% on wet basis. Although the amino acid compositions of leaf and leaf stalk were different each other, glutamic acid and proline were the major amino acids in both of the leaf and leaf stalk. Significantly higher amount (ca.10.3-fold) of free amino acid was present in leaf (3074mg%) than in leaf stalk (298mg%). Glutamic acid and aspartic acid were the major free amino acids and essential amino acid contents in leaf and leaf stalk were 869 and 68mg% being 22.9 and 28.3% of total free amino acids.

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Studies on the Utilization of Persimmons -(Part 1) Effect of Carton Dioxide Treatment on the Removal of Astringency- (감의 이용(利用)에 관(關)한 연구(硏究) -제1보(第一報) 탄산(炭酸)가스처리(處理)가 탈삽(脫澁)에 미치는 영향(影響)-)

  • Seu, Ohn-Soo;Sohn, Tae-Hwa
    • Applied Biological Chemistry
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    • v.19 no.2
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    • pp.93-98
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    • 1976
  • The experiment was made to investigate concentrations of $CO_2\;and\;O_2$ in the removal of astringency of persimmon fruits and to study the changes of total, reducing sugar and hardness during the removal of astringency. 1. During the removal of astringency of persimmon fruits, channel of tannin content showed the rapid decrease in high concentration of $CO_2$ and the gradual decrease in low concentration of $CO_2$. 2. Concentration of oxygen did not show effect on the removal of astringency at high concentration of $CO_2$ but at low concentration of $CO_2$ in Chungdo-Bansi. 3. Optimal concentration of the removal of astringency was $60{\sim}70%,\;CO_2$ and $6{\sim}8%\;O_2$ in Chungdo-Bansi and Sagoksi 4. In changes of sugar content total sugar was decreased and reducing sugar was increased.

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Quality characteristics and antioxidant activity of black Doraji-apple juice mixed with jujube extracts (대추 추출물이 첨가된 흑도라지 사과주스의 품질 특성 및 항산화 활성)

  • Kim, Ja-Min;Moon, Yong-Sun;Yoon, Kyung-Young
    • Food Science and Preservation
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    • v.21 no.2
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    • pp.199-205
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    • 2014
  • This study was conducted to investigate the physicochemical properties and antioxidant activity of black Doragi-apple juice to increase the utilization of Doragi, which is known as a high-functional horticultural crop. To prepare the black Doragi, it was steamed for 15 days at $60^{\circ}C$ and was then dried at $30^{\circ}C$ for 3 h. The five types of black Doragi-apple juice were prepared based on different mixing ratios of black Doraji extract, apple extract, and jujube extract. The mixing ratios of black Doraji extract, apple extract, and jujube extract were 3:3:1, 4:2:1, 2:4:1, 2:2:1, and 2:6:1 for samples A, B, C, D, and E, respectively. The pH and acidity of black Doragi-apple juice were within the ranges of 3.9-4.15 and 1.26-1.51%, respectively. Black Doraji-apple juice E showed the highest sugar content (9.33 $^{\circ}Brix$), reducing sugar content (85.05 mg/mL), and sugar content/acid ratio (6.98). Based on the sensory evaluation, sample C was most preferable in terms of color, taste, sugar-acid ratio, and overall preference, except for the flavor. Black Doragi-apple juice D showed a higher total polyphenol content (706 ${\mu}g/mL$) than sample C (586.22 ${\mu}g/mL$), but there was no statistically significant difference between samples C and D in terms of antioxidant activities. Therefore, it is suggested that the best mixing ratio of black Doraji extract, apple extract, and jujube extract for the production of the best black Doragi-apple juice with excellent taste and antioxidant activities is 2:4:1 (sample C).

Properties of Cellulose as an Enzyme Substrate (효소기질로서의 섬유소의 성질)

  • Kim, B. Hong
    • Microbiology and Biotechnology Letters
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    • v.6 no.4
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    • pp.197-203
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    • 1978
  • Voluminous studies have been carried out on the utilization of cellulosic materials as a raw material for the production of sugar or single cell protein. The structural complexity of the substrate generated problems in the research works as well as in the application of the results obtained in the researches. This review outlines the physical ana chemical properties of cellulosic materials obtained from researches in the field other them biology.

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Spore Production of Entomopathogenic Fungus, Beauveria bassiana 726, Using Molasses (당밀을 이용한 곤충병원성 곰팡이 Beauveria bassiana 726의 포자생산)

  • 김병혁;강성우;윤철식;성재모;홍석인;김승욱
    • KSBB Journal
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    • v.14 no.3
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    • pp.365-370
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    • 1999
  • To optimize the culture conditions for Beauveria bassiana 726, the effects of culture medium, pH, and temperature on mycelium and spore production were investigated. The optimum temperature and pH for the cultivation of B. bassiana 726 were 28 $^{\circ}C$ and 5.0, respectively. The optimized medium was composed of 1.0~2.0% total sugar from molasses, 0.5% corn steep liquor and 0.05% KH$_2$PO$_4$. In the cultivation of B. bassiana 726 with the optimum medium, the specific growth rate and substrate utilization were well-fitted with the proposed kinetic model in the shake flask and stirred tank reactor. When the fed-batch cultivation using carbon suorce, nitrogen source, and mineral salt as a feeding medium was compared with batch cultivation in stirred tank reactor, mycelium (12.7 g/L) and spore production (5.4$\times$$10^8/mL$) were enhanced up to 110% and 85%, respectively.

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UTILIZATION OF $F_1$ HYBRIDS OF FLUE-CURED TOBACCO VARIETIES (N. TARACUM L.) FOR TOBACCO CULTURE BEFORE RICE IN PADDY FIELD ($F_1$잡종을 이용한 황색종 논담배(N. tabacum L.) 품종개량)

  • Jin, Jeong-Eu;Lee, Seung-Cheol;Ko, Mi-Seok
    • Journal of the Korean Society of Tobacco Science
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    • v.7 no.1
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    • pp.33-38
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    • 1985
  • In spite of their inferior quality, pale yellow flue-cured tobacco varieties (N. tabacum L.) are being cultivated as pre-crop of rice in paddy field because of its early leaf maturity. Fertile and male sterile Hicks as green variety , three pale yellow varieties and six single cross $F_1$ hybrids between green and pale yellow varieties, were evaluated. Fertile $F_1$ hybrids were similar to pale yellow varieties in early leaf maturity, curing characters and nicotine contents, where-as they were lower in total nitrogen, higher in reducing sugar comparing to those of the pale yellow varieties.

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Inhibiton Activity and Charaterization of Lactic Acid Bacteria from Pig Feces (돼지분변으로부터 분리한 유산균주들의 헬리코박터 저해력과 항균활성 및 배양특성)

  • Moon, Ki-Hyuke;Park, Phun-Bum;Yoon, Jeong-Weon
    • KSBB Journal
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    • v.20 no.2 s.91
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    • pp.76-83
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    • 2005
  • Lactic acid bacteria were isolated from pig feces for probiotics. The six isolated strains were identified as Lactobacillus paracasei (Lp), Lactobacillus fermentum (Lf), Lactobacillus brevis (Lb), Lactobacillus plantarum (P1 , P2), and Pediococcus pentosaceus (P3) by its sugar utilization, morphological and physiological characteristics. Pl was showed largest antibacterial inhibition zone among the isolated strains. It was against Salmonella gallinarum 25mm, E. coli 20.5mm, Staphylococcus aures 24mm, and Pseudomonas aeruginosa 28mm by inhibitory zone, respectively. Lf was showed hyper acid tolerance, $80\%$ survival rate for 40 minutes, and P1, Lb showed hyper bile tolerance, $408\%,\;283\%$ survival rate for 9 hrs, respectively. Therefore the Lf, P1, and P2 strains were expected to probiotics.

Succinic Acid Production by Anaerobiospirillum succiniciproducens ATCC 29305 Growing on Galactose, Galactose/Glucose, and Galactose/Lactose

  • Lee, Pyung-Cheon;Lee, Sang-Yup;Chan, Ho-Nam
    • Journal of Microbiology and Biotechnology
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    • v.18 no.11
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    • pp.1792-1796
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    • 2008
  • Succinic acid-producing Anaerobinspirillum succiniciproducens was anaerobically grown on galactose, galactose/glucose, or galactose/lactose in order to study its galactose fermentation. Unlike a previous report, A. succiniciproducens was found to efficiently metabolize galactose as the sole carbon source at a rate of 2.4 g/g-DCW/h and produced succinic acid with as high a yield of 87% as with using glucose. When glucose and galactose were present, A. succiniciproducens metabolized both sugars simultaneously. Furthermore, when lactose and galactose coexisted, lactose did not inhibit the galactose fermentation of A. succiniciproducens. Therefore, co-utilization of galactose and other sugars can improve the productivity and economy of bio-based succinic acid processes.