• Imaging Science in Dentistry
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• v.36 no.4
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• pp.189-198
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• 2006

Purpose: To investigate the in vitro response of MC3T3-E1 osteoblastic cells to X-ray in the presence and absence of 2 deoxy-D-glucose (2-DG) and quercetin (QCT). Materials and Methods: The MC3T3-E1 cells were cultured in an ${\alpha}-MEM$ supplemented with 5 mM 2-DG or $10{\mu}M$ QCT and then the cells were incubated for 12 h prior to irradiation with 2, 4, 6, and 8Gy using a linear accelerator (Mevaprimus, Germany) delivered at a rate of 1.5 Gy/min. At various times after the irradiation, the cells were processed for the analyses of proliferation, viability, cytotoxicity, and mineralization. Results: Exposure of the cells to X-ray inhibited the tritium incorporation, 3-(4, 5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT)-reducing activity, and alkaline phosphatase (ALP) activity, and caused cytotoxicity and apoptosis in a dose-dependent manner of the X-ray. This effect was further apparent on day 3 and 7 after the irradiation. RA+2-DG showed the decrease of DNA content, cell viability, and increase of cytotoxicity rather than RA. ALP activity increased on day 7 and subsequently its activity dropped to a lower level. 2-DG suppressed the calcium concentration, but visual difference of number of calcified nodules between RA and RA+2-DG was not noticed. RA+QCT showed the increase of DNA content, cell viability, but decrease of cytotoxicity and subG1 stage cells in the cell cycle, and increased calcified nodules in von Kossa staining rather than the RA. ALP activity showed significant increases on day 7 and subsequently its activity dropped to a lower level. Conclusion: The results showed that the 2-DG acted as a radiosensitizing agent and QCT acted as a radiosensitizing agent respectively in the irradiated MC3T3-E1 osteoblast-like cells.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.7
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• pp.741-753
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• 2008

This paper explores the throughput performance of CSMA/CA-based DCF protocol over both ideal channels and fading channels with payload size at the MAC layer in the 802.11a wireless LAN. In the ideal channel, there are no errors and at the transmission cycle there is one and only one active station which always has a packet to send and other stations can only accept packets and provide acknowledgements. In the fading channel, bit errors appear in the channel randomly and the number of stations is assumed to be fixed. And each station always has packets for transmission. In other words, we operate in saturation conditions. Up to now conventional research work about DCF throughput analysis of IEEE 802.11 a wireless LAN has been done over the ideal channel, but this paper is done over the Rayleigh/Ricean fading channel. So, the ratio of received average energy per bit-to-noise power spectral density $E_b/N_o$ is set to 25 dB and the ratio of direct-to-diffuse signal power in each sub-channel $\xi$ is set to 6 for combined Rayleigh/Ricean fading channel. In conclusion, it is shown that the saturation throughput is always less than the maximum throughput at all the payload size and the higher the transmission rate be, the higher the decreasing rate of saturation throughput compared to the maximum throughput be.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.7 no.4
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• pp.87-100
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• 2012

This study is to determine the key factors of internal situational characteristics, affecting the motivation and performance for overseas expansion of Korean venture business in global markets and to investigate the moderating effect of them on the relationship between entrepreneurship and overseas expansion. To meet the research purpose, by reviewing previous researches, we categorized internal factors into four situational factors of firm size with sales growth, firm age with main product's life cycle, overseas experience and organizational structure and into three entrepreneurship sub-categories of innovativeness, pro-activeness and risk-taking. As result of the statistical analysis of 289 small and medium-sized enterprises with Korean Venture Certificate, it is verified that the motivation for globalization has been influenced by firm size, entrepreneurship, and overseas experience. On the other hand, all factors excepting firm size have positive effects on globalization performance overseas. And, it also verified that there are positive moderating effects of internal situational factors on the relationship of entrepreneurship and the performance of globalization, wheres overseas experience only plays positive moderating effects on the relationship between entrepreneurship and the motivation of globalization. On the basis of these results, we discussed and suggested practical and political issues and implications in order to vitalize globalization of Korean SMEs in the conclusion.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.242-256
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• 2003

This study aimed to determine the relationship between rainfall-discharge patterns and maior aquatic environmental factors in a river-type reservoir. Specifically, daily monitoring was conducted in Paltang Reservoir from January 1999 to December 2001. Observation of the daily changes of the environment factors showed that natural meteorological factors and hydrological factors causing the change of water discharge had a major effect on the aquatic environment. Rainfall was the main source of hydrological changes, with its frequency a possible direct variable governing the range of discharge changes. Rainfall was weak in November${\sim}$May and heavy in June${\sim}$October (heavist in summer). The range of water discharge was greatest during summer (July to September) and lowest during winter (January to February). A principal component analysis (PCA) showed that aquatic environmental factors could be classified into three different types in the pattern of annual variation. First, type I included water temperature, turbidity, water color and organic matter (COD), which increased with increasing water discharge. Second, type ll consisted of DO and pH, which decreased with increasing water discharge. Third, type III included conductivity, alkalinity and chloride ion, which showed middle values with increasing water discharge. Monthly variation of aquatic environments explained by the first two dimensions of the PCA suggests that aquatic environments of Paltang Reservoir may have annual cycle typical of river-type reservoirs depending on hydrological factor such as water discharge.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.26 no.6
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• pp.431-438
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• 2013

The systemization of construction information is required over whole life cycle of facilities to improve productivity of construction industry. BIM(Building Information Modeling) is a technology to manage information based on 3D information model. It has been actively suggested as one of the alternatives. However, it may be currently concentrated on the large project while the small and medium project based on BIM are slightly treated in indifference. In the case of small and medium project, the loss of information has been occurred more seriously than large project. However, it is hard to introduce BIM to the small and medium companies due to the lack of investment resources. This study has been performed to set up information management system based on BIM considering characteristics of small and medium project without excessive investment. In this study, pseudo BIM is defined as BIM for small and medium project. The concept of pseudo BIM has been suggested. The PLIB of ISO and construction information classification system of MOLIT in Korea are used to construct data dictionary for pseudo BIM. A pilot test is performed to verify the effectiveness of pseudo BIM.

• The Korean Journal of Ecology
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• v.25 no.2
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• pp.93-100
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• 2002

In order to analyze the life cycle of A. artemisiifolia var. elation dormancy and some environmental factors inducing germination of the seeds were examined. The results were as follows : Dormancy of fertile seeds was broken in part within a month after seed collection in case of adequate moisture and alternating temperature was also effective in breaking dormancy. The temperature range, which allow germination was 12℃ ∼ 32℃. Optimum temperature for germination was 24℃. The seed of A. artemisiifolia var. elatior was light-independent. The difference of storage period appeared to have no particular effect on the viability of seeds at any time during the 9-month storage period. In the increasing temperature(IT) regime, A artemisiifolia var. elatior seeds started to germinate at 16℃, showing the higher temperature the greater germination rate, the final germination percentage was 99.34%. On the other hand, in the decresing temperature(DT) regime, seeds began to germinate at 20℃ with the 1.34% germination. An induced dormancy occurred at 12℃ making the 5.34% fecal germination in the DT regime. Low temperature was more effective to break dormancy than higher temperature Seeds of A. artemisiifolia var. elatior seems to be germinated in mid to late autumn or germination delayed until following spring. The above results suggest these variation of germination response in diverse environmental factors seems to be a physiological strategy to maintain their existence and to reproduce in the extreme thermal variation.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3519-3528
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• 2012

Inhibitory effects of Maclura amboinenesis Bl, one plant used traditionally for the treatment of cancers, on metastatic potential of highly metastatic B16F10 melanoma cells were investigated in vitro. Cell proliferation was assessed using the MTT colorimetric assay. Details of metastatic capabilities including invasion, migration and adhesion of B16F10 melanoma cells were examined by Boyden Chamber invasion and migration, scratch motility and cell attachment assays, respectively. The results demonstrated that n-hexane and chloroform extracts exhibited potent anti-proliferative effects (p<0.01), whereas the methanol and aqueous extracts had less pronounced effects after 24 h exposure. Bioactivity-guided chromatographic fractionation of both active n-hexane and chloroform extracts led to the isolation of two main prenylated xanthones and characterization as macluraxanthone and gerontoxanthone-I, respectively, their structures being identified by comparison with the spectral data. Interestingly, both exhibited potent effective effects. At non-toxic effective doses, n-hexane and chloroform extracts (10 and $30{\mu}g/ml$) as well as macluraxanthone and gerontoxanthone-I (3 and $10{\mu}M$) significantly inhibited B16F10 cell invasion, to a greater extent than $10{\mu}m$ doxorubicin, while reducing migration of cancer cells without cellular cytotoxicity. Moreover, exposure of B16F10 melanoma cells to high concentrations of chloroform ($30{\mu}g/ml$) and geratoxanthone-I ($20{\mu}M$) for 24 h resulted in delayed adhesion and retarded colonization. As insights into mechanisms of action, typical morphological changes of apoptotic cells e.g. membrane blebbing, chromatin condensation, nuclear fragmentation, apoptotic bodies and loss of adhesion as well as cell cycle arrest in the G1 phase with increase of sub-G1 cell proportions, detected by Hoechst 33342 staining and flow cytometry were observed, suggesting DNA damage and subsequent apoptotic cell death. Taken together, our findings indicate for the first time that active n-hexane and chloroform extracts as well as macluraxanthone and gerontoxanthone-I isolated from Maclura amboinensis Bl. roots affect multistep of cancer metastasis processes including proliferation, adhesion, invasion and migration, possibly through induction of apoptosis of highly metastatic B16F10 melanoma cells. Based on these data, M. amboinensis Bl. represents a potential candidate novel chemopreventive and/or chemotherapeutic agent. Additionally, they also support its ethno-medicinal usage for cancer prevention and/or chemotherapy.

• Journal of Veterinary Clinics
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• v.23 no.2
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• pp.133-143
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• 2006

Diagnosis on reproductive failures of dairy cows by ultrasonography was performed for 151 dairy cows. To diagnose types of reproductive failures, ultrasonography (SA 600, Medison, 5.0 MHz rectal linear transducer) was carried out in combination with rectal examination. Of 151 dairy cows, pregnant cows were 13 and the cows in normal estrual cycle were 40 cows, thereby the cows with reproductive failures were 98 cows. 1. Of 98 cows with reproductive failures, the cows with ovarian diseases were 34 cows (34.7%) and the cows with uterine diseases were 41 cows (41.8%). 2. The diameter of follicle in proestrus was 1.94 cm and it was longer than that of follicle in diestrus (p<0.05). 3. The mean size of corpus luteum of pregnant cows was bigger than that of corpus luteum in normal diestrus (p<0.05). 4. The length of cystic corpus luteum was 3.26 and the width of that was 1.91 cm. The length of corpus luteum tissue was 1.95 and the width of that was 1.91 cm excluding the size of cavity in corpus luteum. 5. The mean length of follicular cyst was 3.31 and the mean width of that was 2.3 cm. 6. The mean length and width of luteal cyst was 3.45 and 2.25 cm, respectively. The mean length and width of corpus luteum tissue was 1.15 and 0.67 cm, respectively, excluding the size of cyst in the luteal cyst. 7. The width of uterine horn associated with endometritis was significantly reduced as the period after parturition was elapsed (p<0.05). The mean width of uterine horn within 40 days after parturition was 4.55 cm. These results indicated that ultrasonography is of great use for accurate diagnosis both on ovarian diseases and uterine diseases and that it is very effective to diagnose endometritis in dairy cows.

• Journal of Life Science
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• v.21 no.6
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• pp.851-857
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• 2011

Sulforaphane (SFN) is an isothiocyanate, isolated from glucoraphanin in broccoli and other cruciferaous vegetables. Recent studies have revealed that SFN induces anti-proliferation and apoptosis by cell cycle arrest in various cancer cells. In this study, we investigated the effect of SFN induced apoptosis in chondrosarcoma HTB-94 cells. SFN caused suppression of proliferation and apoptosis in a dose-dependent manner as determined by cell phenotype, MTT assay and FACS analysis in HTB-94 cells. Treatment of SFN led to caspase-3 activation and p53 accumulation as determined by Western blot analysis. Also, SFN significantly induced DNA fragmentation and nuclear degradation though activation of caspase-3, as detected by DNA electrophoresis and immunostaining, respectively. Our results indicate that SFN-induced apoptosis was regulated by p53 and caspase-3 dependent pathways. Furthermore, SFN may act as a potent anti-proliferation agent, and as a promising candidate for molecular-targeting chemotherapy against human chondrosarcoma cells.

• Journal of Korea Water Resources Association
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• v.39 no.10 s.171
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• pp.881-890
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• 2006

Hydrological characteristics and urbanization effects in the Gap river catchment were investigated employing the SWAT model. The hydrological characteristics analysis showed that total runoff in the whole catchment from 2001 to 2004 consists of 44% of groundwater flow, 6% of lateral flow and 50% of surface flow under year 2000 landuse conditions. The analysis of urbanization effect using different landuse maps for year 1975 and 2000 indicated that although 5% increase in urbanized areas did not significantly impact on the total runoff in the whole catchment, a sub-basin where urbanized area increased by 32% over the past 30 years showed $68{\sim}73%$ decrease in groundwater flow and $22{\sim}66%$ increase in surface flow. It was found that urbanization decreased overall soil moisture and percolation rate except for some increase in soil moisture during dry season. Urbanization effect was found more sensitive during a dry year which has less rainfall and higher evapotranspiration than during a wet year. Therefore, from the results of this study we could infer increased flood damage during wet season and dried stream during dry season due to urbanization. To conclude, the results of this study can provide fundamental information to the eco-friendly restoration project for the three major rivers (Gap-cheon, Yudeung-cheon and Daejeon-cheon) in Daejeon Metropolitan City.