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http://dx.doi.org/10.5352/JLS.2011.21.6.851

Sulforaphane-Induced Apoptosis was Regulated by p53 and Caspase-3 Dependent Pathway in Human Chondrosarcoma, HTB-94  

Lee, Won-Kil (Department of Biological Sciences, College of Natural Sciences, Kongju National University)
Kim, Song-Ja (Department of Biological Sciences, College of Natural Sciences, Kongju National University)
Publication Information
Journal of Life Science / v.21, no.6, 2011 , pp. 851-857 More about this Journal
Abstract
Sulforaphane (SFN) is an isothiocyanate, isolated from glucoraphanin in broccoli and other cruciferaous vegetables. Recent studies have revealed that SFN induces anti-proliferation and apoptosis by cell cycle arrest in various cancer cells. In this study, we investigated the effect of SFN induced apoptosis in chondrosarcoma HTB-94 cells. SFN caused suppression of proliferation and apoptosis in a dose-dependent manner as determined by cell phenotype, MTT assay and FACS analysis in HTB-94 cells. Treatment of SFN led to caspase-3 activation and p53 accumulation as determined by Western blot analysis. Also, SFN significantly induced DNA fragmentation and nuclear degradation though activation of caspase-3, as detected by DNA electrophoresis and immunostaining, respectively. Our results indicate that SFN-induced apoptosis was regulated by p53 and caspase-3 dependent pathways. Furthermore, SFN may act as a potent anti-proliferation agent, and as a promising candidate for molecular-targeting chemotherapy against human chondrosarcoma cells.
Keywords
Chondrosarcoma; sulforaphane; apoptosis; caspase-3;
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