• Journal of Microbiology and Biotechnology
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• v.26 no.3
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• pp.452-460
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• 2016

Acid stress can affect the viability of probiotics, especially Bifidobacterium. This study aimed to improve the acid tolerance of Bifidobacterium longum BBMN68 using adaptive evolution. The stress response, and genomic differences of the parental strain and the variant strain were compared by acid stress. The highest acid-resistant mutant strain (BBMN68m) was isolated from more than 100 asexual lines, which were adaptive to the acid stress for 10^th, 20^th, 30^th, 40^th, and 50^th repeats, respectively. The variant strain showed a significant increase in acid tolerance under conditions of pH 2.5 for 2 h (from 7.92 to 4.44 log CFU/ml) compared with the wild-type strain (WT, from 7.87 to 0 log CFU/ml). The surface of the variant strain was also smoother. Comparative whole-genome analysis showed that the galactosyl transferase D gene (cpsD, bbmn68_1012), a key gene involved in exopolysaccharide (EPS) synthesis, was altered by two nucleotides in the mutant, causing alteration in amino acids, pI (from 8.94 to 9.19), and predicted protein structure. Meanwhile, cpsD expression and EPS production were also reduced in the variant strain (p < 0.05) compared with WT, and the exogenous WT-EPS in the variant strain reduced its acid-resistant ability. These results suggested EPS was related to acid responses of BBMN68.

• Geomechanics and Engineering
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• v.19 no.1
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• pp.79-91
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• 2019

Renewed interest in the long-term pile foundations has been driven by the increase in offshore wind turbine installation to generate renewable energy. A monopile subjected to repetitive loads experiences an evolution of displacements, pile rotation, and stress redistribution along the embedded portion of the pile. However, it is not fully understood how the embedded pile interacts with the surrounding soil elements based on different pile geometries. This study investigates the long-term soil response around offshore monopiles using finite element method. The semi-empirical numerical approach is adopted to account for the fundamental features of volumetric strain (terminal void ratio) and shear strain (shakedown and ratcheting), the strain accumulation rate, and stress obliquity. The model is tested with different strain boundary conditions and stress obliquity by relaxing four model parameters. The parametric study includes pile diameter, embedded length, and moment arm distance from the surface. Numerical results indicate that different pile geometries produce a distinct evolution of lateral displacement and stress. In particular, the repetitive lateral load increases the global lateral load resistance. Further analysis provides insight into the propagation of the shear localization from the pile tip to the ground surface.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.05a
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• pp.275-278
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• 2005

INCONEL 718, nickel based superalloy, has good formability, high strength, excellent corrosion resistance and mechanical properties at high temperature. Owing to theses attractive properties, it finds use in applications such as combustion system, turbine engines and nuclear reactors. In such applications, components are typically required to be tolerant of high stress impact loading. This may cause material degradation and lead to catastrophic failure during service operation. In order to design optimal structural parts made of INCONEL 718, accurate understanding of material's mechanical properties, dynamic behavior and fracture characteristic as a function of strain rates are required. This paper concerned with the dynamic material properties of the INCONEL 718 for the various strain rates. The dynamic response of the INCONEL 718 at intermediate strain rate is obtained from the high speed tensile test machine test and at the high strain rate is from the split Hopkinson pressure bar test. Based on the experimental results, the effects of strain rate on dynamic flow stress, work hardening characteristics, strain rate sensitivity and elongation to the failure are evaluated. Experimental results from both quasi-static and high strain rate up to the 5000/sec are interpolated in order to construct the Johnson-Cook model as the constitutive relation that should be applied to simulate and design the structural parts made of INCONEL 718.

• Transactions of Materials Processing
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• v.14 no.6 s.78
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• pp.559-564
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• 2005

INCONEL 718, nickel based superalloy, has good formability, high strength, excellent corrosion resistance and mechanical properties at high temperature. Owing to theses attractive properties, it is utilized in applications such as combustion system, turbine engines and nuclear reactors. In such applications, components are typically required to be tolerant of high stress impact loading. This may cause material degradation and lead to catastrophic failure during service operation. Accurate understanding of material's mechanical properties with various strain rates is required in order to guarantee the reliability of structural parts made of INCONEL 718. This paper is concerned with the dynamic material properties of the INCONEL 718 at various strain rates. The dynamic response of the INCONEL 718 at the intermediate strain rate is obtained from the high speed tensile test and at the high strain rate is from the split Hopkinson pressure bar test. The effect of the strain rate on dynamic flow stress, work hardening characteristics, strain rate sensitivity and elongation to the failure is evaluated with the experimental results. Experimental results from both the quasi-static and the high strain rate up to 5000/sec are interpolated in order to construct the Johnson-Cook model as the constitutive relation that should be applied to simulate and design the structural parts made of rNCONEL 718.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.90-96
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• 2008

Engineered mixtures composed of rigid sand particles and soft rubber particles are tested to investigate their behavior with strain level. Mixtures are prepared with different volumetric sand fractions (sf) to identify response using small strain resonant column, intermediate strain oedometer, and large strain direct shear tests. The small strain shear modulus and damping ratio are determined with volumetric sand fractions. The asymmetric frequency response curve increases with decreasing sand fraction. Linear responses of shear strain and damping ratio with shear strain are observed at the mixture of sf=0.2. Vertical strain increases with decreasing sand fraction. Mixtures with $04.{\leq}sf{\leq}0.6$ show the transitional stress-deformation behavior from rubber-like to sand-like behavior. The friction angle increases with the sand fraction and no apparent peak strength is observed in mixture without sf=1.0.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.101-105
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• 2003

Recombinant bioluminescent bacteria were used to monitor and classify the to xicity of azo dyes. Two constitutive bioluminescent bacteria, Photobacterium phosphoreum and Es-Cherichia coli, E, coli GC2 (lac::luxCOABE), were used to detect the cellular toxicity of the azo dyes. In addition, four stress-inducible bioluminestent E. coli, DPD2794 (recA::luxCDABE), a DNA damage Sensitive strain; DPD2540 (fabA::luxCDABE), a membrane damage sensitive strain; DPD2511 (katG::luxCDABE), an oxidative damage sensitive strain; and TV1061 (grpE::luxCDABE), a protein damage sensitive strain, were used to provide information about the type of toxicity caused by crystal violet, the most toxic dye of the 16 azo dyes tested. These results suggest that azo dyes result in serious cellular toxicity in bacteria, and that toxicity monitoring and classific ation of some azo dyes, In the field, may be possible using these recombinant bioluminescent bacteria.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1491-1494
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• 2003

A specific experimental method, the Split Hopkinson Pressure Bar (SHPB) technique has been widely used to determine the dynamic material properties under the impact compressive loading conditions with strain-rate of the order of 103/s∼104/s. This type of test procedure has been used to examine the dynamic response of materials in various modes of testing. In this paper, dynamic deformation behaviors of rubber materials widely used for the isolation of vibration from varying structures under dynamic loading are determined using a Split Hopkinson Pressure Bar technique.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.371-376
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• 2004

It is well known that a specific experimental method such as the Split Hopkinson Pressure Bar (SHPB) technique is the simplest experimental technique to determine the dynamic material properties under the impact compressive loading conditions with strain-rate of the order of $10^3/s{\sim}10^4/s$. This type of experimental procedure has been widely used with proper modification on the test setups to determine the varying dynamic response of materials for the dynamic boundary conditions such as tensile and fracture as well. In this paper, dynamic compressive deformation behaviors of an Ethylene Copolymer materials widely used for the isolation of vibration from varying structures under dynamic loading are estimated using the SHPB technique.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.6
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• pp.122-130
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• 2004

It is well known that a specific experimental method, the Split Hopkinson Pressure Bar (SHPB) technique is a best experimental technique to determine the dynamic material properties under the impact compressive loading conditions with strain-rate of the order of 10$^3$/s∼10$^4$/s. This type of experimental procedure has been widely used with proper modification on the test setups to determine the varying dynamic response of materials for the dynamic boundary conditions such as tensile and fracture as well. In this paper, dynamic compressive deformation behaviors of a rubber and an Ethylene Copolymer materials widely used for the isolation of vibration from varying structures under dynamic loading are estimated using a Split Hopkinson Pressure Bar technique.

• Computers and Concrete
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• v.20 no.3
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• pp.361-368
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• 2017

Seismic response of the concrete column covered by nanofiber reinforced polymer (NFRP) layer is investigated. The concrete column is studied in this paper. The column is modeled using sinusoidal shear deformation beam theory (SSDT). Mori-Tanaka model is used for obtaining the effective material properties of the NFRP layer considering agglomeration effects. Using the nonlinear strain-displacement relations, stress-strain relations and Hamilton's principle, the motion equations are derived. Harmonic differential quadrature method (HDQM) along with Newmark method is utilized to obtain the dynamic response of the structure. The effects of different parameters such as NFRP layer, geometrical parameters of column, volume fraction and agglomeration of nanofibers and boundary conditions on the dynamic response of the structure are shown. The results indicated that applied NFRP layer decreases the maximum dynamic displacement of the structure. In addition, using nanofibersas reinforcement leads a reduction in the maximum dynamic displacement of the structure.