Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Response of Bioluminescent Bacteria to Sixteen Azo Dyes

  • Lee, Hwa-Young (National Research Laboratory on Environmental Biotechnology, Kwangju Institute of Science and Technology(K-JIST)) ;
  • Park, Sue-Hyung (National Research Laboratory on Environmental Biotechnology, Kwangju Institute of Science and Technology(K-JIST)) ;
  • Gu, Man-Bock (National Research Laboratory on Environmental Biotechnology, Kwangju Institute of Science and Technology(K-JIST))
  • Published : 2003.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Recombinant bioluminescent bacteria were used to monitor and classify the to xicity of azo dyes. Two constitutive bioluminescent bacteria, Photobacterium phosphoreum and Es-Cherichia coli, E, coli GC2 (lac::luxCOABE), were used to detect the cellular toxicity of the azo dyes. In addition, four stress-inducible bioluminestent E. coli, DPD2794 (recA::luxCDABE), a DNA damage Sensitive strain; DPD2540 (fabA::luxCDABE), a membrane damage sensitive strain; DPD2511 (katG::luxCDABE), an oxidative damage sensitive strain; and TV1061 (grpE::luxCDABE), a protein damage sensitive strain, were used to provide information about the type of toxicity caused by crystal violet, the most toxic dye of the 16 azo dyes tested. These results suggest that azo dyes result in serious cellular toxicity in bacteria, and that toxicity monitoring and classific ation of some azo dyes, In the field, may be possible using these recombinant bioluminescent bacteria.

Keywords

References

  1. Mutat. Res. v.465 The comet assay in eight mouse organs: results with 24 azo compounds Tsuda,S.;N.Matsusaka;H.Madarame;S.Ueno;N.Susa;K.Ishida;N.Kawanura;K.Sekihashi;Y.F.Sasaki https://doi.org/10.1016/S1383-5718(99)00199-0
  2. Mutat. Res. v.462 Genotoxic hazards of azo pigments and other colorants related to 1-phenylazo-2-hydrozynaphthalene Moller,P.;H.Wallin https://doi.org/10.1016/S1383-5742(99)00090-3
  3. Mutat. Res. v.114 The significance of azo-reduction in the mutagenesis and carcinogenesis of azo dyes Chung,K.T. https://doi.org/10.1016/0165-1110(83)90035-0
  4. Carcinogenicity and metabolism of azo dyes, especially those derived from benzidine, U.S. Department of Health and Human Service Publication No.(NIOSH) Boeniger,M.F.
  5. J. Bacteriol. v.172 Monitoring of naphthalene catabolism by bioluminescence with nah-lux transcriptional fusions Burlage,R.S.;G.S.Sayler;F.Larimer https://doi.org/10.1128/jb.172.9.4749-4757.1990
  6. Environ. Toxicol. Chem. v.20 Phenolic toxicity-detection and classification through the use of a recombinant bioluminescent Escherichia coli Choi,S.H.;M.B.Gu https://doi.org/10.1897/1551-5028(2001)020<0248:PTDACT>2.0.CO;2
  7. Mutat. Res. v.442 Distinct responses of a recA::lux CDABE Escherichia coli strain to direct and indirect DNA damaging agents Min,J.;E.J.Kim;R.A.LaRossa;M.B.Gu https://doi.org/10.1016/S1383-5718(99)00059-5
  8. Chemosphere v.46 Toxicity monitoring and classification of endocrine disrupting chemicals(EDCs) using recombinant bioluminescent bacteria Gu,M.B.;J.Min;E.J.Kim https://doi.org/10.1016/S0045-6535(01)00081-9
  9. J. Biotechnol. v.88 Some observations in freeze-drying of recombinant bioluminescent Escherichia coli for toxicity monitoring Gu,M.B.;S.H.Choi;S.W.Kim https://doi.org/10.1016/S0168-1656(01)00268-1
  10. Appl. Environ. Microbiol. v.60 Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens Marincs,F.;D.W.R.White
  11. Appl. Enviton. Microbiol. v.63 no.7 Detection of DNA damage by use of Escherichia coli carrying recA::lux, uvrA::lux, or alkA::lux reporter plasmids Vollmer,A.C.;S.Belkin;D.R.Smulski;T.K.Van dyk;R.A.LaRossa
  12. Appl. Environ. Microbiol. v.62 Oxidative stress detection with Escherichia coli harboring a katG'::lux fusion Belkin,S.;D.R.Smulski;A.C.Vollmer;T.K.Van dyk;R.A.LaRossa
  13. Appl. Environ. Microbiol. v.61 Responses to toxicants of an Escherichia coli strain carrying a uspA'::lux genetic fusion and an E.coli strain carrying a grpE'::lux fusion are similar Van dyk,T.K.;D.R.Smulski;T.R.Reed;S.Belkin;A.C.Vollmer;R.A.LaRossa
  14. Tox. Assess. Int. Q. v.2 Photobacterium phosphoreum toxicity bioassay: 1. Test procedures and applications Ribo,L.M.;K.L.E.Kaiser https://doi.org/10.1002/tox.2540020307
  15. Biotechnol. Bioprocess. Eng. v.5 Toxicity Monitoring of endocrine disrupting chemicals(EDCs) using freeze-dred recombinant bioluminescent bacteria Kim,S.W.;S.H.Choi;J.Min;M.B.Gu https://doi.org/10.1007/BF02932344