• KSCE Journal of Civil and Environmental Engineering Research
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• v.41 no.6
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• pp.637-644
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• 2021

Dynamic response of structures can be evaluated experimentally by conducting cyclic loading tests. It has been known that steel materials are rate-dependent and the lateral response of a structure is significantly affected by the presence of axial force. However, the rate-dependency of steel column structures subjected to both axial and lateral loads has not been sufficiently studied yet due to the difficulty of controlling the axial force in a real-time manner during test. This study introduces an advanced way to apply the axial load in real-time to a column specimen using the adaptive time series (ATS) compensator and the flexible loading beam (FLB), where the H-shape steel columns made of SS275 are used for monotonic and cyclic loading tests with various loading rates with axial loads. The lateral strength and post-yield response of the steel columns are compared for each of monotonic and cyclic loading tests. The estimating equation of yield stress of various strain rate has proposed and finite element analysis were performed for comparison.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.4
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• pp.353-360
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• 2010

In this paper, an efficient algorithm is established in order to estimate the plastic properties of power-law hardening bulk specimen materials with one simple spherical indentation impression test. This work is based on a new formulation of representative strain and, therefore, compare to the preceding approaches the fitting parameters are significantly reduced. Moreover, the new definition of representative strain endowed more physical meaning to the representative strain. In order to verify the reliability of the reverse analysis, we have studied a broad set of materials whose property ranges cover essentially all engineering metals and alloys. Based on the indentation force-displacement P-${\delta}$ curves obtained from numerical simulations, the characteristics of the indentation response and material elastoplastic properties are bridged via explicit functions. Next, through the procedure of reverse analysis the yield stress and power-law hardening exponent of bulk specimen materials can be determined. Finally, good agreement between the result from reverse analysis and initial input data from experiment can be observed.

• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1345-1358
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• 2017

The impact of overproduction of a heterologous protein on the metabolic system of host Lactococcus lactis was investigated. The protein expression profiles of L. lactis IL1403 containing two near-identical plasmids that expressed high- and low-level of the green fluorescent protein (GFP) were examined via shotgun proteomics. Analysis of the two strains via high-throughput LC-MS/MS proteomics identified the expression of 294 proteins. The relative amount of each protein in the proteome of both strains was determined by label-free quantification using the spectral counting method. Although expression level of most proteins were similar, several significant alterations in metabolic network were identified in the high GFP-producing strain. These changes include alterations in the pyruvate fermentation pathway, oxidative pentose phosphate pathway, and de novo synthesis pathway for pyrimidine RNA. Expression of enzymes for the synthesis of dTDP-rhamnose and N-acetylglucosamine from glucose was suppressed in the high GFP strain. In addition, enzymes involved in the amino acid synthesis or interconversion pathway were downregulated. The most noticeable changes in the high GFP-producing strain were a 3.4-fold increase in the expression of stress response and chaperone proteins and increase of caseinolytic peptidase family proteins. Characterization of these host expression changes witnessed during overexpression of GFP was might suggested the metabolic requirements and networks that may limit protein expression, and will aid in the future development of lactococcal hosts to produce more heterologous protein.

• Journal of the Korean Geotechnical Society
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• v.36 no.12
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• pp.77-86
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• 2020

In this study, the importance of implied strength correction for shallow depths at a region of moderate to low seismicity with primary focus on its effect upon site natural period and mean period of the ground motion is investigated. In addition to the most commonly used Modified Kondner-Zelasko (MKZ) model, this paper uses a quadratic/hyperbolic (GQ/H) model that can capture the stress - strain response at large strains as well as small strain stiffness dependence. A total of six site profiles by downhole tests are used and 1D site response analyses are performed using three input motions with contrasting mean periods. The difference between non-corrected and corrected analyses is conditional on the site period as well as mean ground motion period. The effect of periods is analyzed by correlating them with the effective peak ground acceleration, maximum shear strains and amplification factors. The comparative study reveals that the difference is more prominent in soft sites with long site periods. Insignificant differences are observed when soil profiles are subjected to ground motion with very short mean period.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.4
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• pp.23-32
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• 2023

The seismic performance of buried PE pipes is reported to be favorable due to their exceptional elongation capacity at break. Although a seismic performance evaluation procedure based on the response displacement method has been summarized in Korea for fusion-bonded PE pipes, there is currently no procedure available for mechanically jointed PE pipes. This article aims to present a seismic performance evaluation procedure based on the response displacement method specifically designed for mechanically jointed PE pipes in Korea. When employing the mechanical joining method for PE pipes, it is recommended to adhere to the evaluation procedure established for segment-type pipes. This involves assessing the stress induced by the pipe, the expansion and contraction strain of the joint, and the bending angle of the pipe joint. Furthermore, the coefficient of inhomogeneity of the soil, which is necessary for estimating the axial strain of the ground, is introduced. Additionally, a computation method for determining lateral displacement and reconsolidation settlement in soil susceptible to liquefaction is proposed. As a result of the sensitivity analysis considering the typical soil condition in Korea, the mechanically jointed PE pipe with a certain quality was shown to have good structural seismic safety when soil liquefaction was not considered. This procedure serves as a valuable tool for seismic design and evaluating the seismic performance of mechanically joined buried PE pipes, which are primarily utilized for connecting small-diameter pipes.

• Smart Structures and Systems
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• v.19 no.2
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• pp.181-194
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• 2017

In order to investigate the interface debonding defects detection mechanism between steel tube and concrete core of concrete-filled steel tubes (CFSTs), multi-physical fields coupling finite element models constituted of a surface mounted Piezoceramic Lead Zirconate Titanate (PZT) actuator, an embedded PZT sensor and a circular cross section of CFST column are established. The stress wave initiation and propagation induced by the PZT actuator under sinusoidal and sweep frequency excitations are simulated with a two dimensional (2D) plain strain analysis and the difference of stress wave fields close to the interface debonding defect and within the cross section of the CFST members without and with debonding defects are compared in time domain. The linearity and stability of the embedded PZT response under sinusoidal signals with different frequencies and amplitudes are validated. The relationship between the amplitudes of stress wave and the measurement distances in a healthy CFST cross section is also studied. Meanwhile, the responses of PZT sensor under both sinusoidal and sweep frequency excitations are compared and the influence of debonding defect depth and length on the output voltage is also illustrated. The results show the output voltage signal amplitude and head wave arriving time are affected significantly by debonding defects. Moreover, the measurement of PZT sensor is sensitive to the initiation of interface debonding defects. Furthermore, wavelet packet analysis on the voltage signal under sweep frequency excitations is carried out and a normalized wavelet packet energy index (NWPEI) is defined to identify the interfacial debonding. The value of NWPEI attenuates with the increase in the dimension of debonding defects. The results help understand the debonding defects detection mechanism for circular CFST members with PZT technique.

• Korean Journal of Pharmacognosy
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• v.47 no.2
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• pp.179-185
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• 2016

Ginger (Zingiber officnale Roscoe, Zingiberaceae), which is a well-known food seasoning, has been used as a traditional medicine for the treatment of gastrointestinal disorder, vomiting and cough in Korea, China and Japan. Ethanol extract from the dried ginger (DG) was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions the ethyl acetate soluble fraction (EDG) showed the most potent DPPH radical scavenging and superoxide quenching activities. To know the effect of antioxidant activities of EDG, we tested the activities of superoxide dismutase (SOD) and catalase together with oxidative stress tolerance and intracellular ROS level in Caenorhabditis elegans. To investigate whether EDG-mediated increased stress tolerance was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain including CF1553. Consequently, EDG elevated SOD and catalase activities of C. elegans, reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, EDG-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

• Korean Journal of Pharmacognosy
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• v.49 no.1
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• pp.40-46
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• 2018

Chaenomeles sinensis (Thouin) Koehne fruit (Rosaceae) has been used as a traditional medicine in Korea, Japan and China to treat sore throat, diarrhea and inflammation. The ethanol extract of C. sinensis fruit was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions, the n-butanol fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities, the n-butanol fraction was checked the activities of superoxide dismutase (SOD) and catalase activities, and intracellular ROS levels and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of the n-butanol fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, the n-butanol fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, the n-butanol fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

• Korean Journal of Pharmacognosy
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• v.48 no.4
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• pp.298-303
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• 2017

To know the anti-oxidative effect of Epimedii Herba (Berberidaceae), the methanol extract of this plant was investigated by using a Caenorhabditis elegans model system. The methanol extract of this plant showed relatively significant DPPH radical scavenging and superoxide quenching activities. The ethyl acetate soluble fraction of Epimedii Herba (EHE), which showed the most potent DPPH radical scavenging and superoxide quenching activities, was tested on its effects on superoxide dismutase (SOD), catalase, intracellular ROS, and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, in order to verify that regulation of stress-response genes is responsible for the increased stress tolerance of the EHE treated C. elegans, we checked SOD-3 expression using a transgenic strain. As a result, the EHE increased SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Besides, EHE-treated CF1553 worms showed higher SOD-3::GFP intensity than that of non-treated controls.

• International Journal of Industrial Entomology and Biomaterials
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• v.16 no.1
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• pp.21-27
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• 2008

The expression of heat shock protein genes (Hsp 70, Hsp 40, Hsp 20.8 and Hsp 20.4) against thermal stress in silkworm Bombyx mori was performed through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Upon exposure of silkworm to two temperature regimes ($38^{\circ}C$ and $42^{\circ}C$), significant change in the expression of Hsp gene was observed as compared to the control. Hsp 70 and Hsp 40 showed increased expression than the small heat shock protein genes Hsp 20.8 and Hsp 20.4. The Hsp 70 showed increased expression during the recovery period as compared to 1 hr thermal treatments ($38^{\circ}C$/1 hr and $42^{\circ}C$/1 hr). Whereas, Hsp 40, Hsp 20.8 and Hsp 20.4 genes showed higher expression level at initial stages that later gradually decrease during recovery period. Tissue specific expression of Hsp 70 showed variation in the level of expression amongst the tissues. The mid gut and fat body tissues showed higher expression than the cuticle and silk gland tissue. The Hsp 70, Hsp 40 gene expression was analyzed in thermotolerant (Nistari) and thermo susceptible silk worm strain (NB4D2) and results showed significant variation in their expression level. The Nistari showed higher expression of Hsp 70 and Hsp 40 genes than the NB4D2. These findings provide a better understanding of cellular protection mechanisms against environmental stress such as heat shock, as these Hsps are involved in an organism thermotolerance.