• Molecules and Cells
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• v.42 no.11
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• pp.783-793
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• 2019

When endoplasmic reticulum (ER) functions are perturbed, the ER induces several signaling pathways called unfolded protein response to reestablish ER homeostasis through three ER transmembrane proteins: inositol-requiring enzyme 1 (IRE1), PKR-like ER kinase (PERK), and activating transcription factor 6 (ATF6). Although it is important to measure the activity of ATF6 that can indicate the status of the ER, no specific cell-based reporter assay is currently available. Here, we report a new cell-based method for monitoring ER stress based on the cleavage of $ATF6{\alpha}$ by sequential actions of proteases at the Golgi apparatus during ER stress. A new expressing vector was constructed by using fusion gene of GAL4 DNA binding domain (GAL4DBD) and activation domain derived from herpes simplex virus VP16 protein (VP16AD) followed by a human $ATF6{\alpha}$ N-terminal deletion variant. During ER stress, the GAL4DBD-VP16AD(GV)-$hATF6{\alpha}$ deletion variant was cleaved to liberate active transcription activator encompassing GV-$hATF6{\alpha}$ fragment which could translocate into the nucleus. The translocated GV-$hATF6{\alpha}$ fragment strongly induced the expression of firefly luciferase in HeLa Luciferase Reporter cell line containing a stably integrated 5X GAL4 site-luciferase gene. The established double stable reporter cell line HLR-GV-$hATF6{\alpha}$(333) represents an innovative tool to investigate regulated intramembrane proteolysis of $ATF6{\alpha}$. It can substitute active pATF6(N) binding motif-based reporter cell lines.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.3 no.1
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• pp.106-116
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• 1992

This study were purposed to find out parental perceptions of child adjustment associated with parenting stress with autistic children and differences of parenting stress between autistic children's parents and normal children's. Subjects were 27 autistic children's parents, 28 normal children's, 4 teachers with autistic children. 'Scales were Parenting Stress Index(PSI), Eyberg Child Behavior Index(ECBI), Childhood Autism Rating Scale(CARS), Social Maturing Scale(SMS). The results of this study were as follows. 1) In total parenting stress, parents with autistic children had significantly higher scores than normal's. 2) In child domain of parenting stress, fathers with autistic children had significantly higher than mothers on the other components except Mood and Demandingness. 3) In parent domain of parenting stress, parents with autistic children had significantly higher than nomal's on Restrict of role, Attachment, Sense of Competence. All mothers of normal and autistic children had significantly higher than fathers on the other components except Repression. 4) In parenting perceptions associated with parenting stress level of autistic children's parents. Low level group perceived their children as higher adaptibility than teacher's evaluation and high level group perceived them as equal to teacher's.

• Speech Sciences
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• v.11 no.3
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• pp.7-21
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• 2004

The present study aims to investigate domain-initial strengthening phenomenon, which refers to strengthening of articulatory gestures at the initial positions of prosodic domains. More specifically, this paper presents the result of an experimental study of initial syllables with onset consonants (initial-syllable vowels henceforth) of various prosodic domains in English and Hamkyeong Korean, a pitch accent dialect spoken in the northern part of North Korea. The durations of initial-syllable vowels are compared to those of second vowels in real-word tokens for both languages, controlling both stress and segmental environment. Hamkyeong Korean, like English, tuned out to strengthen the domain-initial consonants. With regard to vowel durations, no significant prosodic effect was found in English. On the other hand, Hamkyeong Korean showed significant differences between the durations of initial and non-initial vowels in the higher prosodic domains. The theoretical implications of the findings are as follows: The potentially universal phenomenon of initial strengthening is shown to be subject to language specific variations in its implementation. More importantly, the distinct phonetics- phonology model (Pierrehumbert & Beckman, 1998; Keating, 1990; Cohn, 1993) is better equipped to account for the facts in the present study.

• BMB Reports
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• v.39 no.5
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• pp.607-617
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• 2006

A novel calcium-dependent protein kinase gene (designated as IiCPK2) was cloned from tetraploid Isatis indigotica. The full-length cDNA of IiCPK2 was 2585 bp long with an open reading frame (ORF) of 1878 bp encoding a polypeptide of 625 amino acid residues. The predicted IiCPK2 polypeptide included three domains: a kinase domain, a junction domain (or autoinhibitory region), and a C-terminal calmodulin-like domain (or calcium-binding domain), which presented a typical structure of plant CDPKs. Further analysis of IiCPK2 genomic DNA revealed that it contained 7 exons, 6 introns and the length of most exons was highly conserved. Semi-quantitative RT-PCR revealed that the expression of IiCPK2 in root, stem and leaf were much higher in tetraploid sample than that in diploid progenitor. Further expression analysis revealed that gibberellin ($GA_3$), NaCl and cold treatments could up-regulate the IiCPK2 transcription. All our findings suggest that IiCPK2 might participate in the cold, high salinity and GA3 responsive pathways.

• Plant Biotechnology Reports
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• v.3 no.2
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• pp.127-134
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• 2009

Members of the NAM-ATAF-CUC (NAC) protein family are plant-specific transcription factors that contain a highly conserved N-terminal NAC-domain and diverse C-terminal regions. They have been implicated in plant development and abiotic stress responses. To identify interacters of rice NAC-domain proteins (OsNACs), we performed yeast two-hybrid screening of rice cDNA library using OsNAC5 as a bait, and the results showed that OsNAC5 interacts with other OsNACs including itself. To delineate an interacting domain, a series of deletion constructs of four OsNACs were made and transformed into yeast in various combinations. The results revealed that the conserved NAC domain of OsNACs plays a primary role in homodimer and heterodimer formation, and a part of C-terminal sequence is also necessary for the interaction. In vitro pull-down assays using recombinant OsNAC proteins verified the dimer formations, together suggesting that OsNACs may act by forming homodimers and/or heterodimers in plants.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.484-490
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• 2005

Human ribosomal protein S3 (rpS3), which has a DNA repair endonuclease activity, is a multifunctional protein. This protein is involved in DNA repair, translation, and apoptosis. In particular, rpS3 has a lyase activity, which cleaves the phosphodiester bond of damaged sites such as cyclobutane pyrimidine dimers and AP sites. Here, using deletion analysis, we identified that the repair endonuclease domain resides in the C-terminal region (165-243 aa) of rpS3. We also found that ectopic expression of GST-rpS3 in bacterial strain BL21 promoted the resistance of these cells to ultraviolet (UV) radiation and hydrogen peroxide ($H_{2}O_{2}$) treatment. The repair domain of rpS3 was sufficient to exhibit the resistance to UV irradiation and recover cell growth and viability, showing that the repair activity of rpS3 is responsible for the resistance to UV irradiation. Our study suggests that rpS3 is able to process DNA damage in bacteria via its repair domain, showing the resistance to genotoxic stress. This implies that rpS3-like activity could be operative in bacteria.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.9
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• pp.4239-4249
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• 2011

In the country with frequent earthquakes like Japan, resistance to earthquake is assessed on the basis that Dam body's Face slab is destroyed by concentrated stress. In our country this kind of modeling and analysis is not yet definitely established. This paper performed pseudo static analysis and dynamic analysis for CFRD and evaluated reliability with the results of Shaking Table Test. The Seismic coefficient method, modified seismic coefficient method, Newmark method of Pseudo-static analysis and frequency domain response analysis, time domain history analysis of dinamic analysis were used. The analysis results were differ between analysis method, but the trends of acceleration and displacement were good agreement with the results of shaking table test.

• Coupled systems mechanics
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• v.8 no.5
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• pp.393-414
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• 2019

This paper presents seismic analysis of concrete gravity dams considering soil-structure-fluid interaction. Displacement based plane strain finite element formulation is considered for the dam and foundation domain whereas pressure based finite element formulation is considered for the reservoir domain. A direct coupling method has been adopted to obtain the interaction effects among the dam, foundation and reservoir domain to obtain the dynamic responses of the dam. An efficient absorbing boundary condition has been implemented at the truncation surfaces of the foundation and reservoir domains. A parametric study has been carried out considering each domain separately and collectively based on natural frequencies, crest displacement and stress at the neck level of the dam body. The combined frequency of the entire coupled system is very less than that of the each individual sub-system. The crest displacement and neck level stresses of the dam shows prominent enhancement when coupling effect is taken into consideration. These outcomes suggest that a complete coupled analysis is necessary to obtain the actual responses of the concrete gravity dam. The developed methodology can easily be implemented in finite element code for analyzing the coupled problem to obtain the desired responses of the individual subdomains.

• International journal of advanced smart convergence
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• v.12 no.3
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• pp.109-118
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• 2023

This study attempted to identify the experiences of nursing students' participation in cadaver practice and provide a basis for understanding students' experiences in cadaver practice, which can be reflected in the development of programs for them. By applying the content analysis method according to the guidelines by Krippendorff to analyze the meaning of the experience of participating in cadaver practice among 80 nursing students who participated in cadaver practice at K University in W-si, Gangwon-do, a total of 4 areas, 13 categories, and 25 meaningful statements were derived. The categories included "worried," "surprised," and "fear" in the anxiety domain; "interesting," "knowing," and "focused" in the immersion domain; "value of life," "gratitude and remembrance," "thinking about donation," "facing death," and "precious body" in the reflection domain; and "motivation" and "sense of accomplishment" in the growth domain. The results of this study will help to understand the physical and psychological reactions that nursing students may experience during cadaver practice, and will provide a basis for developing various strategies such as counseling, education, and reflection programs in conjunction with cadaver practice to help nursing students cope with stress, develop a sense of ethical responsibility, and develop a positive self-image as nursing students in order to be successful in cadaver practice. This study is also significant because it provides a basis for preventive program interventions for experiences related to the negative effects of cadaver practice.

• Proceedings of the Materials Research Society of Korea Conference
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• 1994.11a
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• pp.156-162
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• 1994

The existence of the elastic anisotropic channeling is based on the experimental evidences. The rotating electron pairs orbits play the role of basic bonding orbitals. The abnormal signals from ligand domain and train-membrane in cancer cell, Deformation in fatigue and creep at low stress, con duction, superconduction and semiconduction are all from the new metallic bonding orbital.