Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Rice NAC proteins act as homodimers and heterodimers

  • Jeong, Jin Seo (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Park, Yeong Taek (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Jung, Harin (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Park, Su-Hyun (School of Biotechnology and Environmental Engineering, Myongji University) ;
  • Kim, Ju-Kon (School of Biotechnology and Environmental Engineering, Myongji University)
  • Received : 2008.11.03
  • Accepted : 2008.12.15
  • Published : 2009.04.30
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Abstract

Members of the NAM-ATAF-CUC (NAC) protein family are plant-specific transcription factors that contain a highly conserved N-terminal NAC-domain and diverse C-terminal regions. They have been implicated in plant development and abiotic stress responses. To identify interacters of rice NAC-domain proteins (OsNACs), we performed yeast two-hybrid screening of rice cDNA library using OsNAC5 as a bait, and the results showed that OsNAC5 interacts with other OsNACs including itself. To delineate an interacting domain, a series of deletion constructs of four OsNACs were made and transformed into yeast in various combinations. The results revealed that the conserved NAC domain of OsNACs plays a primary role in homodimer and heterodimer formation, and a part of C-terminal sequence is also necessary for the interaction. In vitro pull-down assays using recombinant OsNAC proteins verified the dimer formations, together suggesting that OsNACs may act by forming homodimers and/or heterodimers in plants.

Keywords

Acknowledgement

Supported by : Ministry of Education, Science and Technology

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