• Biotechnology and Bioprocess Engineering:BBE
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• 제5권6호
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• pp.422-430
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• 2000

Mechanism of lignin biodegradation caused by basidiomycetes and the history of lignin biodegradation studies were briefly reviewed. The important roles of fungal extracellular ligninolytic enzymes such as lignin and manganese peroxidases (LiP and MnP) were also summarized. These enzymes were unique in their catalytic mechanisms and substrate specificities. Either LiP or MnP system is capable of oxidizing a variety of aromatic substrates via a one-electron oxidation. Extracellular fungal system for aromatic degradation is non-specific, which recently attracts many people working a bioremediation field. On the other hand, an intracellular degradation system for aromatic compounds is rather specific in the fungal cell. Structurally similar compounds were prepared and metabolized, indicating that an intracellular degradation strategy consisted of the cellular systems for substrate recognition and metabolic response. It was assumed that lignin-degrading fungi might be needed to develop multiple metabolic pathways for a variety of aromatic compounds caused by the action of non-specific ligninolytic enzymes on lignin. Our recent results on chemical stress responsible factors analyzed using mRNA differential display techniques were also mentioned.

• Geomechanics and Engineering
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• 제21권6호
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• pp.527-536
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• 2020

An elastic-plastic solution for cavity expansion problem considering strength degradation, undrained condition and initial anisotropic in-situ stress is established based on the Tresca yield criterion and cavity expansion theory. Assumptions of large-strain for plastic region and small-strain for elastic region are adopted, respectively. The initial in-situ stress state of natural soil mass may be anisotropic caused by consolidation history, and the strength degradation of soil mass is caused by structural damage of soil mass in the process of loading analysis (cavity expansion process). Finally, the published solutions are conducted to verify the suitability of this elastic-plastic solution, and the parametric studies are investigated in order to the significance of this study for in-situ soil test.

• Journal of Cancer Prevention
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• 제23권4호
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• pp.153-161
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• 2018

Imbalance of protein homeostasis (proteostasis) is known to cause cellular malfunction, cell death, and diseases. Elaborate regulation of protein synthesis and degradation is one of the important processes in maintaining normal cellular functions. Protein degradation pathways in eukaryotes are largely divided into proteasome-mediated degradation and lysosome-mediated degradation. Proteasome is a multisubunit complex that selectively degrades 80% to 90% of cellular proteins. Proteasome-mediated degradation can be divided into 26S proteasome (20S proteasome + 19S regulatory particle) and free 20S proteasome degradation. In 1980, it was discovered that during ubiquitination process, wherein ubiquitin binds to a substrate protein in an ATP-dependent manner, ubiquitin acts as a degrading signal to degrade the substrate protein via proteasome. Conversely, 20S proteasome degrades the substrate protein without using ATP or ubiquitin because it recognizes the oxidized and structurally modified hydrophobic patch of the substrate protein. To date, most studies have focused on protein degradation via 26S proteasome. This review describes the 26S/20S proteasomal pathway of protein degradation and discusses the potential of proteasome as therapeutic targets for cancer treatment as well as against diseases caused by abnormalities in the proteolytic system.

• 분석과학
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• 제37권1호
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• pp.25-38
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• 2024

The study aimed to investigate a novel approach by utilizing liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) to separate, identify and characterize very nominal quantities of degradation products (DPs) of balsalazide along with its process related impurities without isolation from their reaction mixtures. The impurities along with balsalazide were resolved on spherisorb ODS2 (250×4.6 mm, 5.0 ㎛) column at room temperature using 0.2 M sodium acetate solution at pH 4.5 and methanol in the ratio of 55:45 (v/v) as mobile phase pumped isocratically at 1.0 mL/min as mobile phase and UV detection at 255 nm. The method shows sensitive detection limit of 0.003 ㎍/mL, 0.015 ㎍/mL and 0.009 ㎍/mL respectively for impurity 1, 2 and 3 with calibration curve liner in the range of 50-300 ㎍/mL for balsalazide and 0.05-0.30 for its impurities. The balsalazide pure compound was subjected to stress studies and a total of four degradation products (DPs) were formed during the stress study and all the DPs were characterized with the help of their fragmentation pattern and the masses obtained upon LC-MS/MS. The DPs were identified as 3-({4-[(E)-(4-hydroxyphenyl) diazenyl]benzoyl}amino)propanoic acid (DP 1), 4-[(E)-(4-hydroxyphenyl)diazenyl] benzamide (DP 2), 5-[(E)-(4-carbamoylphenyl)diazenyl]-2-hydroxybenzoic acid (DP 3) and 3-({4-[(E)-phenyldiazenyl]benzoyl}amino)propanoic acid (DP 4). Based on findings, it was concluded that, the proposed method was successfully applicable for routine analysis of balsalazide and its process related impurities in pure drug and formulations and also applicable for identification of known and unknown impurities of balsalazide.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회A
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• pp.108-113
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• 2007

In this report, stress corrosion cracking generation due to pipe material degradation in the primary stage of the nuclear power plant was investigated. Firstly, after artificially degrading the CF8A steel during 2, 4, and 6 months in actual temperature, $400^{\circ}C,$ assessed corrosion susceptibility of the degraded material following ASTM G5 standard. And next, the S.C.C. tests for the degraded material were conducted under the condition of $60^{\circ}C,$ 2wt.% H2BO3+Li70H solution, 0.8 oy. From the results, Corrosion rates linearly increased with degradation period and solution temperature increase. And both the raw material and the degraded materials were not failed in the S.C.C. test condition. In spite of long time test (about 3,900 hrs) under S.C.C. condition, surface pits or surface corrosion by the electro chemical reaction were not observed. And also, even though the nondestructive DCPD and ACPD methods were applied to on-line monitor the S.C.C. failure processes it was impossible because the surface pits and cracks were not generated.

• Molecules and Cells
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• 제38권5호
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• pp.390-395
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• 2015

Degradation of chlorophyll (Chl) by Chl catabolic enzymes (CCEs) causes the loss of green color that typically occurs during senescence of leaves. In addition to CCEs, STAYGREEN1 (SGR1) functions as a key regulator of Chl degradation. Although sgr1 mutants in many plant species exhibit a staygreen phenotype, the biochemical function of the SGR1 protein remains elusive. Many recent studies have examined the physiological and molecular roles of SGR1 and its homologs (SGR2 and SGR-LIKE) in Chl metabolism, finding that these proteins have different roles in different species. In this review, we summarize the recent studies on SGR and discuss the most likely functions of SGR homologs.

• Advances in aircraft and spacecraft science
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• 제11권1호
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• pp.1-21
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• 2024

The stiffness reduction of cross-ply composite laminates featuring a transverse cracking and delamination within the mid-layer is predicted through utilization of a modified shear-lag model, incorporating a stress perturbation function. Good agreement is obtained by comparing the prediction models and experimental data. The material characteristics of the composite are affected by fluctuations in temperature and transient moisture concentration distribution in desorption case, based on a micro-mechanical model of laminates. The transient and non-uniform moisture concentration distribution induces a stiffness reduction. The obtained results demonstrate the stiffness degradation dependence on factors such as cracks density, thickness ratio and environmental conditions. The present study underscores the significance of comprehending the degradation of material properties in the failure progression of laminates, particularly in instances of extensive delamination growth.

• BMB Reports
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• 제50권8호
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• pp.393-400
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• 2017

Plants are constantly exposed to a variety of abiotic stresses, such as drought, heat, cold, flood, and salinity. To survive under such unfavorable conditions, plants have evolutionarily developed their own resistant-mechanisms. For several decades, many studies have clarified specific stress response pathways of plants through various molecular and genetic studies. In particular, it was recently discovered that ubiquitin proteasome system (UPS), a regulatory mechanism for protein turn over, is greatly involved in the stress responsive pathways. In the UPS, many E3 ligases play key roles in recognizing and tethering poly-ubiquitins on target proteins for subsequent degradation by the 26S proteasome. Here we discuss the roles of RING ligases that have been defined in related to abiotic stress responses in plants.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1995년도 가을 학술발표회 논문집
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• pp.185-193
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• 1995

The softening behaviors of concrete have been the object of numerous experimental and numerical studies, because the load carrying capacity of cracked concrete structure is not zero. Numerical studies are devoted to the investigation of three-dimensional softening behaviors of concrete on the basis of a viscoplastic theory, which may be able to represent the effects of plasticity and also of rheology. In order to properly describe material behaviors corresponding to different stress levels, two surfaces in stress space are adopted; one is a yield surface, and the other is a failure or bounding surface. When a stress path reaches the failure surface, it is considered that the softening behaviors are initiated as micro-cracks coalesce and are simulated by assuming that the actual strain increments in the post-peak region are less than the equivalent viscoplastic strain increment. The experimental studies and the finite element analyses have been carried out under the displacement control. Numerically simulated results indicate that the model is able to predict the essential characteristics of concrete behaviors such as the non-linearity, stiffness degradation, different behaviors in tension and compression, and specially dilatation under uniaxial compression.

• International Journal of Concrete Structures and Materials
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• 제10권4호
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• pp.435-450
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• 2016

Experimental studies were conducted on 36 grouted splices to investigate their mechanical performance under four loading schemes: (1) incremental tensile loading, (2) repeated tensile loading, (3) cyclic loading at high stress, and (4) cyclic loading at large strain. Load-deformation responses of the grouted splices under cyclic loadings were featured with pinching effect and stiffness degradation compared to those responses under tensile loadings. The shape of the hysteresis loops of load-deformation curves was similar to that under incremental tensile loading. For the purpose of structural analysis, stress-strain relationships were presented for grouted splices under various loadings.