• 한국콘텐츠학회논문지
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• 제7권3호
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• pp.101-109
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• 2007

한국과학 영재학교 학생들과 민족사관고 학생들의 여가활동 실태를 규명하기 위하여 영재학교 166명 (남:122, 여:44), 민사고 184명(남:71, 여:113)을 대상으로 분석하였다. 1) 남학생들은 스포츠에 여학생의 경우 영재학교는 취미교양 활동에 민사고는 관람 감상활동에 가장 많이 참여하는 것으로 나타났다. 2) 여가활동을 하게 된 직접적인 동기는 정신적 스트레스와 갈등 해소라는 응답이 가장 많았고, $75\sim80%$정도가 여가활동이 건강과 체력 유지에 아주 중요한 역할을 하는 것으로 인식하고 있었다. 3) 혼자서 여가 활동을 한다는 비율이 30%정도였고, 계획 없이 여가활동을 실시한다는 응답도 영재학교 남학생 51%, 여학생 40%, 민사고는 남학생 40%, 여학생 48% 정도인 것으로 제시되었다. 4) 평일에 주로 영재학교 남학생들은 스포츠 활동에 참여하였고, 민사고 남 여학생 및 영재학교 여학생 집단은 취미 교양활동에 가장 많이 참여하였다. 5) 남학생의 경우 영재학교 38%, 민사고기 54% 학생들이 평일에 컴퓨터 게임을 하지 않는다고 응답하였고, 여학생은 70% 정도가 컴퓨터 게임을 하지 않는 것으로 나타났다. 영재학교 남학생들이 민사고 남학생들 보다 컴퓨터 게임에 더 많은 시간을 소비하였다. 6) 여가 활동을 하지 않는 가장 큰 이유로 시험이나 과제로 인한 시간부족을 꼽았고, 게을러서 여가 활동을 하지 않는다는 응답도 $19\sim25%$나 되었다.

• 생명과학회지
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• 제32권6호
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• pp.482-490
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• 2022

근육 양과 근력의 감소에 의한 근감소증은 다양한 병적 상태의 악화, 삶의 질 저하, 사망률의 증가를 동반하며, 기대수명이 증가함에 따라 앞으로도 유병률은 계속 증가할 것이다. 노화 과정에서 만성 산화 스트레스와 염증 반응의 증가는 골격근 소실의 주요 원인으로 작용하며, 에너지 대사에 필수적인 미토콘드리아의 기능 장애와 관련된 자가포식 및 세포사멸 신호의 교란은 근육 단백질의 손실을 가속화한다. 오래전부터 각종 질병의 예방 및 치료에 널리 사용되어온 동충하초의 주요 생리활성물질인 cordycepin의 약리학적 효과는 항산화 및 항염증 작용과 직접적인 관련이 있는 것으로 알려져 있다. 본 총설에서는 근감소증의 예방과 치료에의 적용을 위한 cordycepin의 세포사멸, 자가포식, 단백질의 이화작용 및 근육 재생에 중요한 위성세포의 활성에 대한 연관성을 제시하였다. 비록 현재까지 근감소증에 대한 cordycepin의 연구는 미진한 수준이지만, 그동안의 연구 결과에서 cordycepin은 노화로 인한 미토콘드리아 기능 약화를 억제하고 근육 단백질의 분해를 차단하는 데 기여할 수 있음을 알 수 있다. 또한 근세포 손상에 대한 cordycepin의 보호 효과는 항산화 및 항염증 활성과 밀접한 관련이 있음을 제안한다. 따라서 근세포의 노화방지에 관여하는 cordycepin의 분자생물학적 기전을 중심으로 보다 지속적인 기초 연구가 필요할 것으로 사료된다.

• Biomolecules & Therapeutics
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• 제31권1호
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• pp.97-107
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• 2023

Aristolochic acid (AA), extracted from Aristolochiaceae plants, plays an essential role in traditional herbal medicines and is used for different diseases. However, AA has been found to be nephrotoxic and is known to cause aristolochic acid nephropathy (AAN). AA-induced acute kidney injury (AKI) is a syndrome in AAN with a high morbidity that manifests mitochondrial damage as a key part of its pathological progression. Melatonin primarily serves as a mitochondria-targeted antioxidant. However, its mitochondrial protective role in AA-induced AKI is barely reported. In this study, mice were administrated 2.5 mg/kg AA to induce AKI. Melatonin reduced the increase in Upro and Scr and attenuated the necrosis and atrophy of renal proximal tubules in mice exposed to AA. Melatonin suppressed ROS generation, MDA levels and iNOS expression and increased SOD activities in vivo and in vitro. Intriguingly, the in vivo study revealed that melatonin decreased mitochondrial fragmentation in renal proximal tubular cells and increased ATP levels in kidney tissues in response to AA. In vitro, melatonin restored the mitochondrial membrane potential (MMP) in NRK-52E and HK-2 cells and led to an elevation in ATP levels. Confocal immunofluorescence data showed that puncta containing Mito-tracker and GFP-LC3A/B were reduced, thereby impeding the mitophagy of tubular epithelial cells. Furthermore, melatonin decreased LC3A/B-II expression and increased p62 expression. The apoptosis of tubular epithelial cells induced by AA was decreased. Therefore, our findings revealed that melatonin could prevent AA-induced AKI by attenuating mitochondrial damage, which may provide a potential therapeutic method for renal AA toxicity.

• 대한치위생과학회지
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• 제1권2호
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• pp.41-52
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• 2018

The purpose of this study was to examine the state of oral prophylaxis practice among dental hygiene students and their awareness of musculoskeletal diseases in an effort to provide some information on how to strengthen education on treatment posture to manage musculoskeletal diseases and how to raise awareness of musculoskeletal diseases. From November 2 to 13, 2016, a self-administered survey was conducted on 653 sophomores, juniors and seniors with an experience of oral prophylaxis practice. SPSS version 20.0 for Windows was employed to analyze the collected data. The findings of the study were as follows: 1. The largest group that accounted for 37.4% responded that the total number of students undergoing oral prophylaxis practice during a semester was four to six. The biggest group that represented 65.4% answered that the required practice time per student was one to fewer than three hours. 76.0% continued to be in the repeated same posture. 2. As for the posture of patients, supine position was most common for the maxillary sinus, which accounted for 82.2%. And semi-upright position was most common for the mandibular sinus, which represented 49.6%. 3. In regard to the burden of oral prophylaxis practice, 33.9% considered the required for the practice appropriate. 42.3% took the repeated long-lasting posture, and 53.5% were under physical pressure. 55.4% suffered from mental pressure and stress. 4. The most dominant musculoskeletal area that they experienced pain after oral prophylaxis practice was neck with 52.5%; waist with 48.2, shoulders/wrists/hands with 45.5, back with 10.3, buttocks with 4.1, elbows with 2.3, legs with 2.1, ankles/feet with 0.8 and knees with 0.6%. 5. Concerning the maintenance of repeated treatment postures and pain experience, the students who continued to be in the repeated same position underwent more pain than the others who didn't on the shoulders(2.92±1.05), in the waist(3.02±1.01), buttocks(1.75±0.92), elbows(1.55±0.79) and ankles/foot(2.52±1.25). The differences were statistically significant(p<.05, p<.01). 6. As to educational experience on treatment posture and musculoskeletal diseases, 88.8% received education on treatment posture; 87.9%, on what position should be taken in times of cooperation; 46.9%, on musculoskeletal diseases; 51.9%, on carpal tunnel syndrome; 42.3%, on varicose vein. The students who replied education on occupational diseases was necessary accounted for 89.6%. 7. The students who experienced treatment posture education were better aware of the causes of musculoskeletal diseases(3.23±3.00), ways for preventing the diseases(3.33±.834) and how to stretch the body(3.63±.858). The differences were statistically significant(p<.05, p<.001). 8. The students who experienced education on occupational diseases heard more about musculoskeletal diseases(3.27±.965), were better cognizant of the causes of the diseases(3.45±.847), were better aware of how to prevent them(3.55±.805) and found themselves to know how to stretch to prevent the diseases (3.73±.826). The differences were statistically significant(p<.001).

• 대한치과보철학회지
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• 제52권2호
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• pp.82-89
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• 2014

연구 목적: 본 연구의 목적은 중합 과정 중 치과용 레진 시멘트의 점탄성 성질의 변화를 관찰하기 위한 것이다. 연구 재료 및 방법: 6 종류의 레진시멘트(Clearfil SA luting, Panavia F 2.0, Zirconite, Variolink N, RelyX Unicem clicker, RelyX U200)가 이번 실험에 사용되었다. AR1500 stress controlled rheometer를 이용해 $32^{\circ}C$에서 동적 시간 경과 시험(dynamic oscillation time sweep test)이 시행되었다. 각각의 레진시멘트의 전단 저장 계수(G'), 전단 손실 계수(G"), 손실 탄젠트(tan ${\delta}$), 변위량을 20분 동안 3번씩 반복 측정하였다. 측정 결과는 일원배치분석 및 Tukey's hoc test로 사후 검정을 시행하였다(${\alpha}$=0.05). 결과: 모든 레진 시멘트는 혼합 후 시간에 따라 G' 값이 증가하였고, 최종적으로 안정상태에 도달하였다. 실험 종료 시점에서 RelyX U200은 가장 높은 G'값을 나타냈고, RelyX Unicem (clicker type)과 Variolink N이 가장 낮은 G'값을 나타냈다. Tan ${\delta}$와 변위량은 일정 수준의 값을 유지하다가 G'이 증가하기 시작하는 시점에서 0에 도달하였다. 이는 변위량이 0에 도달하는 지점과 거의 일치하였으며, 그 시간은 6분 내외였다. 결론: 본 연구에서 RelyX U200은 다른 레진 시멘트와 비교하여 가장 오랜 시간 동안 소성을 유지하고, 경화 완료 후 가장 높은 강도(rigidity)를 보였다. 따라서 여러 개의 보철물을 동시에 합착해야 하는 경우에 RelyX U200이 유용할 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3903-3907
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• 2013

Background: Patients with colorectal cancer are usually treated with chemotherapy, which reduces the number of blood cells, especially white blood cells, and consequently increases the risk of infections. Some research studies have reported that aromatherapy massage affects the immune system and improves immune function by, for example, increasing the numbers of natural killer cells and peripheral blood lymphocytes. However, there has been no report of any study which provided good evidence as to whether aromatherapy with Thai massage could improve the immune system in patients with colorectal cancer. The objectives of this study were to determine whether the use of aromatherapy with light Thai massage in patients with colorectal cancer, who have received chemotherapy, can result in improvement of the cellular immunity and reduce the severity of the common symptoms of side effects. Materials and Methods: Sixty-six patients with colorectal cancer in Phichit Hospital, Thailand, were enrolled in a single-blind, randomised-controlled trial. The intervention consisted of three massage sessions with ginger and coconut oil over a 1-week period. The control group received standard supportive care only. Assessments were conducted at pre-assessment and at the end of one week of massage or standard care. Changes from pre-assessment to the end of treatment were measured in terms of white blood cells, neutrophils, lymphocytes, CD4 and CD8 cells and the CD4/CD8 ratio and also the severity of self-rated symptom scores. Results: The main finding was that after adjusting for pre-assessment values the mean lymphocyte count at the post-assessment was significantly higher (P=0.04) in the treatment group than in the controls. The size of this difference suggested that aromatherapy with Thai massage could boost lymphocyte numbers by 11%. The secondary outcomes were that at the post assessment the symptom severity scores for fatigue, presenting symptom, pain and stress were significantly lower in the massage group than in the standard care controls. Conclusions: Aromatherapy with light Thai massage can be beneficial for the immune systems of cancer patients who are undergoing chemotherapy by increasing the number of lymphocytes and can help to reduce the severity of common symptoms.

• Journal of the Korean Data and Information Science Society
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• 제27권6호
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• pp.1609-1619
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• 2016

본 연구는 한국형 웰니스 생활양식 측정도구의 구성타당도와 신뢰도를 확인하기 위한 도구개발 연구이다. 간호대학생 345명이 99문항의 WEL (wellness evaluation of lifestyle)을 작성하였고, SPSS WIN 22.0와 AMOS 18.0을 이용한 탐색적 요인분석과 확인적 요인분석을 통해 측정도구의 구성타당도를 검증하였다. 최종 한국형 웰니스 생활양식 측정도구는 71문항, 14개 하위척도로 구성되었다. 각 하위척도는 4개의 요인구조를 가지는 것으로 확인되었다: 본질적 자기, 사회적 자기, 신체적 자기, 적응적 자기, 모델의 적합도는 ${\chi}^2=225.12$, p<.001, CMIN/DF=3.17, RMSEA=.08, NFI=.87, IFI=.91, CFI=.91로 나타났다. 도구의 집중타당도를 평가한 결과 기각비 (C.R.)는 2.95~16.84로였고, 표준분산추출 (AVE)도 .61~.69로 수용 가능하였다. 하위척도의 내적 일치도 Cronbach's ${\alpha}$는 .55~.87이었다. 본 연구를 통해 한국형 웰니스 생활양식 측정도구가 한국인의 웰니스를 다차원적으로 측정하기에 타당하고 신뢰성있는 도구임을 확인하였다.

• 대한한의학방제학회지
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• 제25권4호
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• pp.509-526
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• 2017

Background and objectives : Soeumin Bojungykgi-tang (seBYTE) has been used to supplement qi in Korean medicine. It has been demonstrated to possess various biological functions such as anti-cancer, anti-aging and anti-inflammatory effects. The present study evaluated the protective roles of seBYTE in hepatotoxic in vitro and in vivo model. Methods : To investigate cytoprotective effect of seBYTE, HepG2 cells were pretreated with seBYTE and then subsequently exposed to $10{\mu}m$ AA for 12 h, followed by $5{\mu}m$ iron. Cell viability was examined by MTT assay, and expression of apoptosis-related proteins was evaluated by immunoblot analysis. For responsible molecular mechanisms, ROS production, GSH contents, and mitochondrial membrane potential were measured. In addition, hepatoprotective effect of seBYTE in vivo was assessed in $CCl_4$-induced animal model. Results : seBYTE prevented AA + iron-induced cytotoxicity in concentration dependent manner. In addition, ROS production, GSH depletion, and mitochondrial dysfunction induced by AA + iron were significantly reduced by seBYTE pretreatment. Furthermore, seBYTE recovered expression of the pro-apoptotic proteins such as PARP and pro-caspase-3. In animal experiment, plasma ALT and AST levels were significantly elevated in $CCl_4$ treatment, but seBYTE significantly decreased the ALT and AST levels. Moreover, seBYTE alleviated the numbers of histological activity index, percentages of degenerative regions, degenerated hepatocytes, infiltrated inflammatory cells, nitrotyrosine- and 4-hydroxynonenal-positive cells in liver. Conclusions : These results showed that hepatoprotective effect of seBYTE against on $CCl_4$-induced hepatic damages is partly due to antioxidative and anti-apoptotic process.

• 생명과학회지
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• 제20권2호
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• pp.245-252
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• 2010

실험동물은 생후 4주령 Sprague Dawley계 수컷 흰쥐 30마리로 유산소운동+부추섭취군(6마리), 유산소운동군(6마리), 부추섭취군(6마리), 대조군(6마리)으로 총 24마리로 구분하였다. 운동은 총 2주간 트레드밀로 실시하였으며, 유산소 운동은 주5회, 1일 25~30분 실시하였다. 부추는 5%를 첨가한 사료를 섭취시킨 결과 다음과 같은 결론을 얻었다. 혈중지질에서 TC와 TG는 집단간 유의한 차이가 없었다. HDL-C는 유산소운동+부추섭취군, 유산소운동군, 부추섭취군이 대조군보다 높았다. LDL-C는 유산소운동+부추섭취군, 유산소운동군과 부추섭취군이 대조군 보다 낮았다. MDA는 유산소운동군과 부추섭취군이 대조군보다 낮았다. 항산화 효소에서 SOD의 활성도는 유산소운동군과 부추섭취군이 대조군보다 높았다. CAT의 활성도는 유산소운동+부추섭취군이 대조군보다 높았다. 낮은 강도의 유산소 운동과 천연 항산화제인 부추섭취는 지질과산화물을 낮춤으로서 산화적 스트레스를 개선하는 효과가 있는 것으로 사료된다. 향후 부추섭취와 관련하여 운동강도에 대한 지속적인 연구가 요구되어진다.

• Toxicological Research
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• 제22권1호
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• pp.23-30
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• 2006

Among poly aromatic hydrocarbons, dioxin and PCBs are the most controversial environmental pollutants in our modern life. These pollutants are known as human carcinogens, and liver is the most sensitive target in animal cancer models. Specific aims of the study were focused on the mechanism of carcinogenesis in hepatocytes and the structure-activity relation among these diverse environmental chemicals. Because key mechanisms of dioxin-induced carcinogenesis in human epithelial cell model are the alteration of signal transduction pathway and PKC isoforms, the alteration of the signal transduction pathways and other factors associated with carcinogenesis were studied. Rat hepatocytes cultured under the sandwich protocols were exposed with the various concentration of dioxins and PCBs, and signal transduction pathway, protein kinase C isoforms, oxidant stress, and apoptotic nuclei were evaluated. Since it is important to understand the structure-activity relation among these chemicals to properly assess the carcinogenic potentials, the study analyzed the parameters associated with carcinogenic processes, based on their structural characteristics. In addition, signal transduction pathways and PKC isoforms involved in inhibition of UV-induced apoptosis were also analyzed to elaborate the tumor promotion mechanism of these chemicals. Induction of apoptosis by UV irradiation was optimal at $60\;J/m^2$ in primary hepatocyte in culture. Compared to non coplanar PCBs such as PCB 114 and PCB 153, coplanar PCBs such as PCB 77 and PCB126 showed a stronger inhibition of apoptosis induced by UV irradiation. Production of reactive oxygen species (ROS) was more stimulated by non-coplanar PCBs than coplanar PCBs with the most potent induction of ROS by chlorinated non-coplanar PCB. As compared to the level of induction by PCB126, non-coplanar PCB153 showed a higher increase of intracellular concentrations. Besides the alteration of intracellular calcium concentration, translocation of PKC from cytosolic fraction to membrane fraction was clearly observed upon the exposure of non-coplanar PCB. Taken together, the present study demonstrated that there is a potent structure-activity relationship among PCB congeners and the mechanism of PAH-induced carcinogenesis is structure-specific. The study suggested that more diverse pathways of PAH-induced carcinogenesis should be taken into account beyond the boundary of Ah receptor dogma to assess the health impact of PAH with more accuracy.