• The Journal of Korean Medicine
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• v.25 no.3
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• pp.137-148
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• 2004

Objectives : To study change of external dermal formation, change of leukocytes in vasculature, change of lipid formation in stratum corneum and distribution of ceramide through administering Sihocheonggan-san (SC) extract on to the control (CON). This study was done through forcing injury to mice's back skin which have lipid protection formation in stratum corneum. Materials and Methods : The CON to which damage was caused intentionally using the external application on the mice's back skin used the SC. The change of leukocytes in vasculature were identified through optima 5.2 and Student's t-test and the results were made into a dermal formation graph. Results : After dispensing SC extract into the CON, dermal injury was decreased. Especially, recover of lipid protection formation, which includes lipid and ceramide in stratum corneum suppressing acute inflammation that some factors are PKC, TNF-α, IL-12B which controlled the secretion of relating inflammatory cytokine, also went onto decrease of angiogenesis, and a decrease of degranulated mast cells was noted. In addition, the decrease of epithelial injury also caused the growth of cells to decrease in stratum basale and cytoclasis. In the vasculature, the leukocytes were also decreased and it could relate to decrease in CON. Conclusions : SC has effect on CON suppressing the dermal injury through recovering of lipid protection formation in stratum corneum.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.2
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• pp.99-104
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• 2007

The skin barrier function is one of the vital functions. Horny layer and epidermis among various skin layers play an important role in protecting body from environmental insults. Stratum corneum is completely formed by differentiation processes from the basal layer and newly formed corneocytes push the older ones from skin surface. In this study we investigated changes of various parameters - capacitance, transepidermal water loss (TEWL), pH and amount of removed corneocytes - according to the stratum corneum's depth. We removed the stratum corneum using tape stripping technique. We found that uppermost stratum corneum play an important role in skin surface moisturization and lower stratum corneum in protecting transepidermal water loss. In addition, skin pH is more acidic than surface pH. In the stratum corneum, skin pH is mote acidic in the deeper layer. Furthermore amount of removed corneocyte is also decreased according to depth because of stronger cell-cell union strength. From these results we suggest that stratum corneum has different characteristics depending on their position.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.2
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• pp.131-138
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• 2019

Carbonylated proteins (CPs) are synthesized by the chemical reaction of basic amino acid residues in proteins with aldehyde compounds yielded by lipid peroxidation. CPs are excited by a range of light from UVA to blue light, and resulted in the generation of superoxide anion radicals ($^{\cdot}O_2{^-}$) by photosensitizing reaction. Then, they CPs induce new protein carbonylation in stratum corneum through ROS generation. Furthermore, the superoxide anion radicals produce CPs in the stratum corneum (SC) through lipid peroxidation and finally affects skin conditions including color and moisture functions. The purpose of this study was to investigate the relationship between the production of stratum corneum carbonylated protein (SCCP) and the skin elasticity. 46 healthy female Koream at the ages of 30 ~ 50 years old were participated in this study for 8 weeks. The skin test was experiment conducted into two groups; placebo group (N = 23) used cream that did not contain active ingredients, and the other group (N = 23) used cream containing the elasticity improving ingredients. Test areas were the crow 's feet and the cheek. Various non-invasive methods were carried out to measure biophysical parameters on human skin indicating that dermis density and skin wrinkle were measured by using DUB scanner and Primos premium, respectively. Skin elasticity were measured using dermal torque meter (DTM310) and balistometer (BLS780). SCCP was assessed in a simple and non-invasive method using skin surface biopsy on the cheek of the subject. The amount of SCCP was determined using image analysis. All measurements were taken at 0, 4 and 8 8week. Results revealed that the amount of CP in SC was reduced when the skin wrinkle and skin elasticity related parameters were improved. This indicates that the correlation between the elasticity improvement and the amount of CP can be used as a anti-aging indicator and applicable to the skin clinical test for the measurement of skin aging in the future.

• Proceedings of the SCSK Conference
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• 1999.10a
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• pp.132-132
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• 1999

In the cosmetics field, facial skins have been classified into four types according to self estimation as well as physiological parameters. The aim of this study is to understand skin condition in the levels and composition of stratum corneum (SC) lipids, and to propose new classification for skin types. We assessed the relationship between the SC lipid composition and the self-estimated skin types or physiological parameters of the skin. Sensitive skin has been of great concern over the last decades, and it should be recognized as a skin type. Therefore, we also investigated the influence of the SC lipid composition on variations of sensitivity evaluated by the Stinging Test. Fifty-five healthy Japanese women aged 22-44 participated in this study. Skin biopsies were taken from facial skin using polyethylene sheet with cyanoacrylate. SC lipids were extracted and separated into individual lipid classes. The combined ceramides and cholesterol were quantified by HPTLC. Free fatty acid was quantified according to the ACS-ACOD method. Instrumental measurements were made at the site around the biopsy. In addition, the Stinging Test and a sensory evaluation questionnaire were administered to all subjects. The generally recognized O-D skin type classification is dependent on the consumer`s subjective assessment with respect to their skin troubles. The product of hydration state and skin surface lipid level showed a significant correlation with self-estimated skin types. The oily-dry skin type classified according to physiological parameters as well as SC lipid levels should be characterized as delicate skin with its barrier function deteriorating in the winter. Two groups of sensitive skin were established. One is the barrier function of the skin is deteriorated, and another is the sebaceous gland activity is in decline.

• Journal of Veterinary Clinics
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• v.27 no.2
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• pp.142-146
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• 2010

The purpose of this study is to establish experimental canine skin barrier disruption model in dog. The study was designed to investigate the predictive properties of acetone damage using as effect parameters transepidermal water loss (TEWL) and stratum corneum (SC) hydration. To compare the structures of SC intercellular lipids in normal and acetone damaged skin, TEM observations were performed. Six clinically normal, male Beagles without dermatological problems were chosen for this study. Acetone damage was performed at 48hrs after clipping. Efficacy measurements for TEWL and skin hydration were performed before ($t_1$) and after ($t_2$) damage in a temperature- and humidity-controlled room ($2{0\sim}22^{\circ}C$, 50-60%). TEWL and SC hydration values were decreased in the acetone damaged model compared with non damaged skin. In TEM observation of acetone damaged sample, the intercellular lipid lamellae exhibit abnormal and incomplete structure compared with those of normal skin. It seems that acetone damage would be one of canine skin epidermal barrier disruption model for the study of canine atopic dermatitis (AD) as well as dry skin in veterinary dermatology research.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.387-412
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• 2003

Influence of gelatinase on basement membrane (BM) structure was investigated by using a skin equivalent (SE) model. The results showed that (1) gelatinase produced by cells degraded the BM and (2) the addition of matrix metalloproteinase-specific inhibitor to the SE medium accelerated the formation of BM structure, indicating that gelatinase is involved in BM impairment. The activity of gelatinase was also studied in healthy human facial skin tissues. The result of in situ zymography revealed gelatinase activity around the basal layer of the epidermis, where BM integrity was severely compromised. Therefore, this enzyme was suggested to be associated with BM decomposition in human facial skin. To assess the behavior of gelatinase in stratum corneum (SC) non-invasively, an immunological study was performed. Since positive immunostaining of pro-gelatinase B was observed in SC stripped from sun-exposed skin, whereas no positive staining detected in SC of non-irradiated skin, gelatinase in the epidermis could be non-invasively detected by measuring gelatinase in SC. Gelatinase in SC of healthy female volunteers was monitored using a special film that sensitively and conveniently detects gelatinase. Ninetr percent of SC from facial skin (l00 women, 40's-50's) was gelatinase-positive. On the other hand, SC from non-irradiated skin was negative. These results strongly suggest that (1) gelatinase is constantly produced in the facial epidermis of most middle-aged woman during their daily life, and (2) the enzyme might be involved in the aging-related degeneration of both BM and the matrix fibers of the upper layer of the dermis, acting as a very important aging factor. Strong inhibitory activity against gelatinase was found in turmeric extract and identified curcumin as the major ingredient. Topical application of cream containing turmeric extract significantly decreased the number of gelatinase-positive SC clusters in human facial skins. These results indicated that turmeric is an effective ingredient to prevent skin from photo aging by suppressing chlonically upregulated gelatinase activity by UV and to improve skin condition.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.81-87
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• 2018

The objective of this article is to provide analytical tools for the scattering of stratum corneum (SC) and to check whether the optical clearing agents (OCAs) could be applied in optics affecting the scattering reduction. Dark field images of tape striped corneocyte separates scattered light of the SC from others in vitro. Several optical clearing agents were tested to reduce the scattering. Physical properties of SC such as water contents, keratin configuration and volume after OCAs treatment were investigated by FT-IR and 3D laser microscope. Several reducing sugars, monomeric sugars, sugar alcohol, and hyaluronic acid, which were used as humectants in cosmetic field, also reduced scattering. However, unlike dehydration in optics, water penetrated into the keratin in SC and scattering was decreased at low concentration of OCAs. In that condition, the volume of corneocyte was increased and stiffness seemed to decrease. The analyzing of tape-stripped SC, showed the change of optical and physical properties of corneocyte by optical clearing agents. The hydration of SC layer by optical clearing agents decreased the scattering of corneocyte and thus improved the skin appearance and moisturizing effect, which are important benefits in the cosmetic field and could provide new possibility to develop skin care study targeting at SC.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.2
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• pp.113-119
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• 2015

The measurement of hydration level in the surface layer of the skin, stratum corneum (SC), gives important information on the biophysical properties and function of the skin barrier such as softness, flexibility, and healthiness of the skin. But it is difficult to measure a consistent hydration level from a sample to another sample due to individual variations and environmental changes. The aim of this study was to evaluate objective hydration after using various products in the SC. The SC Hydration was measured by capacitance (Corneometer$^{(R)}$, C+K, Germany) on the ventral site of forearm from 40 healthy volunteers. The skin surface was chronologically measured immediately after application of the test products and 3 and 6 hours later. We analyzed the averages of five measurements of each site and used the hydration increase rate for correction on untreated site variation. We found that most polyols including glycerol and butylenes glycol influenced directly the hydration increase rate in the SC previously. In this study, glycerol was used to prepare the standard products from 0 to 20 percents and applied to the same volunteers. The individual standard curve showed linear relation to glycerol concentrations. Based on the the standard curve, hydration of SC was converted into hydration increase rate to glycerol concentrations. The converted glycerol concentrations of products were repetitive and reproducible. In addition, the individual standard curve was used to relate the skin type of each individual. These results suggest that the hydration of the SC standardized regardless of external variation and individual skin condition can explain detailed skin state variation. Further studies will be conducted with other ingredients such as surfactants, lipids and aqueous materials, and with other methods for noninvasive measurement.

• The Journal of the Convergence on Culture Technology
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• v.3 no.3
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• pp.31-36
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• 2017

We are made up to the variation of the structure modeling of the stratum corneum by the skin on the hydration. The given skin structure is consist of the control level of the intercellular route and transcellular route, and is confirmed to the variation of the modeling for algorithm. Hydration condition is consisted to modeling by the size, form and combination, that skin impedance is appeared to result the value of measurement by the several layer such as forms of ${\xi}-R-SC-RH$, ${\xi}-R-SL-RH$, ${\xi}-R-SG-RH$, ${\xi}-R-SS-RH$ and ${\xi}-R-SB-RH$. Hydration condition is showed to the alteration difference value of S-Corneum, S-Lucidum, S-Granulosum, S-Spinosum and S-Basale. And, composition condition was constructed with the alteration modeling that was established to separate the division parts for conversion system. We will be possible to progress the improvement effectiveness of the skin and to consist of the continuous hydration control system for functional on the skin. We will be possible to progress the improvement effectiveness of the skin and to consist of the continuous penetration control system for functional energy on the skin.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.1
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• pp.27-39
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• 1996

Recently, in vitro stratum curneum model was a useful tool to evaluate the skin condition such as tranespidermal water loss, skin hydration and etc.. In this study to evaluate the alteration of SC with aging in hairless mouse, we measured TEWL using in vitro SC model, SC lipid, and outer corneocytes area. Although the total SC lipid was rich about 30% in 8 week old mouse compared with that of 82 week old mouse, the TEWL values were higher in the former than that of the latter. The outer corneocytes area was 559 $\pm$ 65 $mu extrm{m}$2 in 8 week old mouse and 755 $\pm$ 56 ${\mu}{\textrm}{m}$2 in 82 week old mouse. So the barrier function was reinforced with aging. This result suggested that the reinforced barrier function is one of the defense systems against the outer enviornment and the decreased skin function with aging.