Browse > Article

Transepidermal Water Loss, Stratum Corneum Hydration and Transmission Electron Microscope Observation on Acetone Damaged Canine Skin Barrier Model  

Oh, Won-Seok (College of Veterinary Medicine, Kyungpook National University)
Park, Seong-Jun (Neodin Veterinary Science Institute)
Oh, Tae-Ho (College of Veterinary Medicine, Chungnam National University)
Publication Information
Journal of Veterinary Clinics / v.27, no.2, 2010 , pp. 142-146 More about this Journal
Abstract
The purpose of this study is to establish experimental canine skin barrier disruption model in dog. The study was designed to investigate the predictive properties of acetone damage using as effect parameters transepidermal water loss (TEWL) and stratum corneum (SC) hydration. To compare the structures of SC intercellular lipids in normal and acetone damaged skin, TEM observations were performed. Six clinically normal, male Beagles without dermatological problems were chosen for this study. Acetone damage was performed at 48hrs after clipping. Efficacy measurements for TEWL and skin hydration were performed before ($t_1$) and after ($t_2$) damage in a temperature- and humidity-controlled room ($2{0\sim}22^{\circ}C$, 50-60%). TEWL and SC hydration values were decreased in the acetone damaged model compared with non damaged skin. In TEM observation of acetone damaged sample, the intercellular lipid lamellae exhibit abnormal and incomplete structure compared with those of normal skin. It seems that acetone damage would be one of canine skin epidermal barrier disruption model for the study of canine atopic dermatitis (AD) as well as dry skin in veterinary dermatology research.
Keywords
Canine; Skin barrier; Acetone; TEM; TEWL; stratum corneum hydration;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Fluhr JW, Dickel H, Kuss O, Weyher I, Diepgen TL, Berardesca E. Impact of anatomical location on barrier recovery, surface pH and stratum corneum hydration after acute barrier disruption. Br J Dermatol 2002; 146: 770-776.   DOI   ScienceOn
2 Fuchs SM, Fluhr JW, Maibach HI. Barrier Recovery. In: Bioengineering of the skin: Water and the Stratum Corneum, 2nd ed. Boca Raton: CRC Press. 2005: 197-209
3 Inman AO, Olivry T, Dunston SM, Monteiro-Riviere NA, Gatto H. Electron microscopic observations of stratum corneum intercellular lipids in normal and atopic dogs. Vet Pathol 2001; 38: 720-723.   DOI   ScienceOn
4 Linde YW. Dry skin in atopic dermatitis. Acta Derm Venereol Suppl (Stockh) 1992; 177: 9-13.
5 Madison KC, Swartzendruber DC, Wertz PW, Downing DT. Presence of intact intercellular lipid lamellae in the upper layers of the stratum corneum. J Investig Dermatol 1987; 88: 714-718.   DOI   ScienceOn
6 Bikle DD, Ratnam A, Mauro T, Harris J, Pillai S. Changes in calcium responsiveness and handling during keratinocyte differentiation. Potential role of the calcium receptor. J Clin Invest 1996; 97: 1085-1093.   DOI
7 Di Nardo A, Wertz P, Giannetti A, Seidenari S. Ceramide and cholesterol composition of the skin of patients with atopic dermatitis. Acta Derm Venereol 1998; 78: 27-30.   DOI   ScienceOn
8 Edelberg R. Relation of electrical properties of skin to structure and physiologic state. J Invest Dermatol 1977; 69: 324-327.   DOI   ScienceOn
9 Fartasch M. Ultrastructure of the epidermal barrier after irritation. Microsc Res Tech 1997; 37: 193-199.   DOI   ScienceOn
10 Elias PM. Epidermal lipids, barrier function, and desquamation. J Invest Dermatol 1983; 80 (Suppl): 44s-49s.
11 Mao-Qiang M, Mauro T, Bench G, Warren R, Elias PM, Feingold KR. Calcium and potassium inhibit barrier recovery after disruption, independent of the type of insult in hairless mice. Exp Dermatol 1997; 6: 36-40.   DOI   ScienceOn
12 Mauro T, Isseroff R, Lasarow R, Pappone P. Ion channels are linked to differentiation in keratinocytes. J Membr Biol 1993; 132: 201-209.
13 Menon GK, Elias PM, Lee SH, Feingold KR. Localization of calcium in murine epidermis following disruption and repair of the permeability barrier. Cell Tissue Res 1992; 270: 503-512.   DOI   ScienceOn
14 Miyamoto T, Nojima H, Shinkado T, Nakahashi T, Kuraishi Y. Itch-associated response induced by experimental dry skin in mice. Jpn J Pharmacol 2002; 88: 285-292.   DOI
15 Motta S, Monti M, Sesana S, Mellesi L, Ghidoni R, Caputo R. Abnormality of water barrier function in psoriasis. Role of ceramide fractions. Arch Dermatol 1994; 130: 452-456.   DOI   ScienceOn
16 Pinnagoda J, Tupker RA, Coenraads PJ, Nater JP. Prediction of susceptibility to an irritant response by transepidermal water loss. Contact Dermatitis 1989; 20: 341-346.   DOI   ScienceOn
17 Zhai H, Leow YH, Maibach HI. Human barrier recovery after acute acetone perturbation: An irritant dermatitis model. Clin Exp Dermatol 1998; 23: 11-13.   DOI   ScienceOn