Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Transepidermal Water Loss, Stratum Corneum Hydration and Transmission Electron Microscope Observation on Acetone Damaged Canine Skin Barrier Model

아세톤에 의해 손상된 개 피부장벽 모델에서의 경표피수분소실도 및 각질층보습도 평가와 전자현미경적 관찰

  • Oh, Won-Seok (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Seong-Jun (Neodin Veterinary Science Institute) ;
  • Oh, Tae-Ho (College of Veterinary Medicine, Chungnam National University)
  • 오원석 (경북대학교 수의과대학) ;
  • 박성준 ((주)네오딘동물의학연구소) ;
  • 오태호 (충남대학교 수의과대학)
  • Accepted : 2010.04.09
  • Published : 2010.04.30
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Abstract

The purpose of this study is to establish experimental canine skin barrier disruption model in dog. The study was designed to investigate the predictive properties of acetone damage using as effect parameters transepidermal water loss (TEWL) and stratum corneum (SC) hydration. To compare the structures of SC intercellular lipids in normal and acetone damaged skin, TEM observations were performed. Six clinically normal, male Beagles without dermatological problems were chosen for this study. Acetone damage was performed at 48hrs after clipping. Efficacy measurements for TEWL and skin hydration were performed before ($t_1$) and after ($t_2$) damage in a temperature- and humidity-controlled room ($2{0\sim}22^{\circ}C$, 50-60%). TEWL and SC hydration values were decreased in the acetone damaged model compared with non damaged skin. In TEM observation of acetone damaged sample, the intercellular lipid lamellae exhibit abnormal and incomplete structure compared with those of normal skin. It seems that acetone damage would be one of canine skin epidermal barrier disruption model for the study of canine atopic dermatitis (AD) as well as dry skin in veterinary dermatology research.

본 연구는 실험적으로 개의 피부장벽 손상 모델을 평가하기 위해 아세톤을 이용하여 개 피부장벽의 손상을 유도하고 손상의 정도를 경표피수분소실과 각질층보습도 수치를 측정하였다. 손상 후의 각질층세포간 지질구조는 전자현미경을 이용하여 평가하였다. 피부 및 임상병리학적 검사에서 건강한 2~4세령의 수컷 비글 개 6마리를 사용하였으며 아세톤에 의해 피부는 삭모 후 48시간후 에 손상되었다. 경표피수분소실도와 각질층보습도는 아세톤 손상전보다 손상후에 유의성 있게 저하되었다. 아세톤 손상표피의 전자현미경학적 평가에서는 손상된 각질세포간 비정상적이고 불완전한 지질층판이 관찰되었다. 본 연구를 통하여 아세톤을 이용한 개의 피부장벽손상은 개 아토피나 건성피부 연구를 위한 손상모델 가능성이 있는 것으로 판단된다.

Keywords

References

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