• Title/Summary/Keyword: strand orientation

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Characteristics of Surface Strand Orientation and Strand Mat Thickness Variation and Its Effect on the Bending Properties of Commercial OSB

  • Oh, Sei Chang
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.2
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    • pp.56-62
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    • 2008
  • The surface and edge structure of OSB is defined by surface strand orientation and strand mat thickness variation parallel to the length of the panel using video-microscope. The bending strength of OSB was correlated with surface strand orientation and decreased with increasing the orientation angle in the direction parallel to length of the panel. Average strand mat thickness variation parallel to the length of the panel did not influence the bending strength, but the bonding characteristics among the outermost strands affects the bending strength of OSB. Hankinson formula can be used to predicts the MOE according to strand orientation in the surface of OSB, and more precise strand alignment and reducing thickness variation should be important in the structural performance of OSB panels.

DNA Light-strand Preferential Recognition of Human Mitochondria Transcription Termination Factor mTERF

  • Nam, Sang-Chul;Kang, Chang-Won
    • BMB Reports
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    • v.38 no.6
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    • pp.690-694
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    • 2005
  • Transcription termination of the human mitochondrial genome requires specific binding to termination factor mTERF. In this study, mTERF was produced in E. coli and purified by two-step chromatography. mTERF-binding DNA sequences were isolated from a pool of randomized sequences by the repeated selection of bound sequences by gel-mobility shift assay and polymerase chain reaction. Sequencing and comparison of the 23 isolated clones revealed a 16-bp consensus sequence of 5'-GTG$\b{TGGC}$AGANCCNGG-3' in the light-strand (underlined residues were absolutely conserved), which nicely matched the genomic 13-bp terminator sequence 5'-$\b{TGGC}$AGAGCCCGG-3'. Moreover, mTERF binding assays of heteroduplex and single-stranded DNAs showed mTERF recognized the light strand in preference to the heavy strand. The preferential binding of mTERF with the light-strand may explain its distinct orientation-dependent termination activity.

Cloning and Characterization of a Gene Encoding 22 kDa Functional Protein of Bacteriophage MB78

  • Gupta, Lalita;Chakravorty, Maharani
    • BMB Reports
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    • v.38 no.2
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    • pp.161-166
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    • 2005
  • Functional protein of MB78 bacteriophage having apparent molecular weight of 22 kDa is expressed from 1.7 kb HindIII G fragment. The nucleotide sequence of this fragment showed two open reading frames of 222 and 196 codons in tail-to-tail orientation separated by a 62-nucleotide intercistronic region. The ORF of 22 kDa protein is present in opposite orientation, i.e. in the complementary strand, preceded by a strong ribosomal binding site and a promoter sequence. Another ORF started from the beginning of the fragment whose promoter region and translational start site lies in the 0.45 kb HincII U fragment which is located next to the HindIII G fragment, that has the sequence for DNA bending. 3' end of the fragment has high sequence homology to the EaA and EaI proteins of bacteriophage P22, a close relative of MB78 phage.

Exploring Reliability of Oriented Strand Board's Tensile and Stiffness Strengths

  • Wang, Y.;Young, T.M.;Guess, F.M.;Leon, R.V.
    • International Journal of Reliability and Applications
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    • v.8 no.1
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    • pp.111-124
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    • 2007
  • In this paper, we apply insightful statistical reliability tools to manage and seek improvements in the strengths of Oriented Strand Board (OSB). As a part of the OSB manufacturing process, the product undergoes destructive testing at various intervals to determine compliance with customers' specifications. Workers perform these tests on sampled cross sections of the OSB panel to measure the tensile strength, also called internal bond (IB), in pounds per square inches until failure. Additional stiffness strength tests include parallel and perpendicular elasticity indices (EI), which are taken from cross sectional samples of the OSB panel in the parallel and perpendicular directions with respect to the orientation of the wood strands. We explore both graphically and statistically these "pressure-to-failures" of OSB. Also, we briefly comment on reducing sources of variability in the IB and EI of OSB.

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Constitutive equations for polymer mole and rubbers: Lessons from the $20^{th}$ century

  • Wagner, Manfred H.
    • Korea-Australia Rheology Journal
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    • v.11 no.4
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    • pp.293-304
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    • 1999
  • Refinements of classical theories for entangled or crosslinked polymeric systems have led to incommensurable models for rubber networks and polymer melts, contrary to experimental evidence, which suggests a great deal of similarity. Uniaxial elongation and compression data of linear and branched polymer melts as well as of crosslinked rubbers were analyzed with respect to their nonlinear strain measure. This was found to be the result of two contributions: (1) affine orientation of network strands, and (2) isotropic strand extension. Network strand extension is caused by an increasing restriction of lateral movement of polymer chains due to deformation, and is modelled by a molecular stress function which in the tube concept of Doi and Edwards is the inverse of the relative tube diameter. Up to moderate strains, $f^2$ is found to be linear in the average stretch for melts as well as for rubbers, which corresponds to a constant tube volume. At large strains, rubbers show maximum extensibility, while melts show maximum molecular tension. This maximum value of the molecular stress function governs the ultimate magnitude of the strain-hardening effect of linear and long-chain branched polymer melts in extensional flows.

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Reflections on the Elementary School Geometry Curriculum in the Netherlands -Based on the Realistic Mathematics Education- (네덜란드의 초등학교 기하 교육과정에 대한 개관 -현실적 수학교육을 중심으로-)

  • Chong, Yeong-Ok
    • School Mathematics
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    • v.9 no.2
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    • pp.197-222
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    • 2007
  • The study aims to reflect the elementary school geometry education based on the Realistic Mathematics Education in the Netherlands in the light of the results from recent researches in geometry education and the direction of geometry standards for school mathematics of the National Council of Teachers of Mathematics in order to induce implications for improving korean geometry curriculum and textbook series. In order to attain these purposes, the present paper reflects the history of elementary school geometry education in the Netherlands, sketches the elementary school geometry education based on the Realistic Mathematics Education in the Netherlands by reflecting general goals of the mathematics education, the core goals for geometry strand of the Netherlands, and geometry and spatial orientation strand of Dutch Pluspunt textbook series for the elementary school more concretely. Under these reflections on the documents, it is analyzed what is the characteristics of geometry strand in the Netherlands as follows: emphasis on realistic spatial phenomenon, intuitive and informal approach, progressive approach from intuitive activity to spatial reasoning, intertwinement of mathematics strands and other disciplines, emphasis on interaction of the students, cyclical repetition of experiencing phase, explaining phases, and connecting phase. Finally, discussing points for improving our elementary school geometry curriculum and textbook series development are described as follows: introducing spatial orientation and emphasizing spatial visualization and spatial reasoning with respect to the instruction contents, considering balancing between approach stressing on grasping space and approach stressing on logical structure of geometry, intuitive approach, and integrating mathematics strands and other disciplines with respect to the instruction method.

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Roles of Budding Yeast Hrr25 in Recombination and Sporulation

  • Lee, Min-Su;Joo, Jeong Hwan;Kim, Keunpil
    • Journal of Microbiology and Biotechnology
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    • v.27 no.6
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    • pp.1198-1203
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    • 2017
  • Hrr25, a casein kinase $1{\delta}/{\varepsilon}$ homolog in budding yeast, is essential to set up mono-orientation of sister kinetochores during meiosis. Hrr25 kinase activity coordinates sister chromatid cohesion via cohesin phosphorylation. Here, we investigated the prophase role of Hrr25 using the auxin-inducible degron system and by ectopic expression of Hrr25 during yeast meiosis. Hrr25 mediates nuclear division in meiosis I but does not affect DNA replication. We also found that initiation of meiotic double-strand breaks as well as joint molecule formation were normal in HRR25-deficient cells. Thus, Hrr25 is essential for termination of meiotic division but not homologous recombination.

Anterior Cruciate Ligament Double Bundle Reconstruction with Hamstring Tendon Autografts - Technical Notes (자가 슬괵건을 이용한 전방 십자 인대 이준 다발 재건술 - 수술 술기 -)

  • Ahn, Jin-Hwan;Lee, Sang-Hak;Ahn, Hyung-Kwon;Kang, Hong-Jae
    • Journal of the Korean Arthroscopy Society
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    • v.9 no.2
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    • pp.222-231
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    • 2005
  • Purpose: This article describes a double-bundle ACL reconstruction technique using a five-strand hamstring tendon autograft with conventional anteromedial bundle reconstruction and additional posterolateral bundle reconstruction. Operative technique: For the tibial tunnel, the conventional single tunnel technique is performed and for the femoral tunnel, the double tunnel technique is performed with the anteromedial and posterolateral bundle. After minimal notchplasty, the anteromedial femoral tunnel is prepared with leaving one milimeter of posterior femoral cortex within the over-the-top, which if positioned at the 11-o'clock orientation for the right knee or at the 1-o'clock position for the left knee. The posterolateral femoral tunnel that is located 5 to 7 mm superior to the inner margin of the lateral meniscus anterior horn at $90^{\circ}$ of flexion is prepared with tile outside-in technique using a 4.5 cannulated reamer. The graft material for the double bundle reconstruction is made of the conventional four-strand hamstring autograft in the anteromedial bundle and of a single-strand semitendinosus tendon in the posterolateral bundle. The anteromedial bundle is fixed with using a rigid fix system on the femoral side and the posterolateral bundle is fixed to tie with the miniplate from the outside femur. Then, with the knee in $10^{\circ}\;to\;20^{\circ}$ of flexion, a bioabsorbable screw is simultaneously applied to achieve tibial fixation with tensioning of both bundles. Conclusion: A double bundle reconstruction with five-strand hamstring autograft, which is designed with a favorable conventional anteromedial bundle and an additional posterolateral bundle to restore rotation stability, seems to be a very effective method for the treatment for ACL instabilities.

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A Study on Fatigue Crack Propagation Behavior in Random Short-Fiber SMC Composites (비규칙 단섬유강화 SMC 복합재료의 피로균열 전파거동에 관한 연구)

  • Kim, Jae-Dong;Koh, Sung-Wi
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.26 no.2
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    • pp.204-212
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    • 1990
  • The SMC composite, now being considered in certain structural applications, is anticipated to experience repeated loading during service. Thus, understanding of the fatigue behavior is essential in proper use of the composite material. In this paper, using the SMC composite composed of E-glass chopped strand and unsaturated polyester resin three point bending fatigue tests are carried out to investigate the fatigue crack propagating behavior under various cyclic stresses and fatigue damage of various microcrack forms. The following results are obtained from this study; 1) Most of the total fatigue life of the SMC composite is consumed at the initial extension or the growth of the macroscopic crack. 2) A Paris' type power-law relationship between the crack propagation rate and stress intensity factor range is obtained, and the value of material constant m is much higher (m=9~11)than that of other metals. 3) In case of high cyclic stress the fatigue damage show high microcrack density and short crack length, but in case of low cyclic stress does it vice versa. 4) Fatigue damage is characterized by microcrack density, crack length and distribution of crack orientation.

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Light environment and physiological response of Panax ginseng I. Experience on light in natural habitat,through cultivation under forest and artificial shade, and change of light control technique (인삼의 광환경과 생리반응 I.자생지. 임간 및 일복 재배에서 광경험과 광조절 방법의 변천)

  • Park, Hoon
    • Journal of Ginseng Research
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    • v.7 no.2
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    • pp.172-192
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    • 1983
  • Light environment and growth of Panax ginseng In habitat and cultivation under natural shade were renewed. Grower's experiences on ginseng stand In relation to light environment were assessed. Change of shading method and grower's concepts on light requirement of ginseng plant in Korea, Manchuria and Japan was counted. Growth of wild ginseng was better under rich light. Optimum crown density index appeared to be 0.7 In natural habitat and 0.4 to 0.7 for the cultivation in forest. Change of light Intensity in forest was greatest in May and reached near to constant value (from 40% to 3% for broad leaf deciduous forest and loom 4% to 2% for pine forest). Insufficient light condition induced long and thin stem, poor flowering and seed bearing, and sequent dormancy. Relation between light and ginseng strand was not clear but light Interception with cool weather was effective. Topography and orientation of bed have been deeply considered for light environment. Panel or bark of won were used for shading in deep forest manly In Manchuria while reed blind and straw thatch were used in Korea. Kinds and number of shades material and seasonal or daily schedule have been simplified probably by labor pressure due to eulargement of plantation. Post height has been greater while width of roof, bed and ditch has changed lisle. Scientific survey in the past omitted important light control methods (complete light hardening etc) which has been practiced in specific areas. The concept and technique of light control in the past in Korea were delicate and intensive including the control of temperature and moisture. For the application of old concept in modem cultivation precise Investigation of the technique including the measurement of light, temperature and moisture is needed.

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