• Molecular & Cellular Toxicology
• /
• v.1 no.2
• /
• pp.99-105
• /
• 2005

Perchloroethylene (tetrachloroethylene, PCE), a dry cleaning and degreasing solvent, can enter ground-water through accidental leak or spills. PCE can be degraded to trichloroethylene (TCE), 1, 1-dichloroethylene (DCE) and vinyl chloride (VC) as potential bio-product. These compounds have been reported that they can cause clinical diseases and cytotoxicity. However, only a little genotoxic information of these compounds has been known. In this study, we investigated DNA single strand breaks of PCE, TCE, DCE and VC by single cell gel electrophoresis assay, (comet assay) which is a sensitive, reliable and rapid method for DNA single strand breaks with mouse lymphoma L5178Y cells. From these results, $37.5\;{\mu}g/ml$ of PCE, $189\;{\mu}g/ml$ of TCE and $56.4\;{\mu}g/ml$ of DCE were revealed significant DNA damages in the absence of S-9 metabolic activation system meaning direct-acting mutagen. And in the presence of S-9 metabolic activation system, $41.5\;{\mu}g/ml$ of PCE, $328.7\;{\mu}g/ml$ of TCE and $949\;{\mu}g/ml$ of DCE were induced significant DNA damage. In the case of VC, it was revealed a significant DNA damage in the presence of S-9 metabolic activation system. Therefore, we suggest that chloroethylene compounds (PCE, TCE, DCE and VC) may be induced the DNA damage in a mammalian cell.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.17 no.2
• /
• pp.220-226
• /
• 2016

Because MS(Multi-Strand) cables consist of many strands, a jacking force is applied to each strand one by one for cable construction. All strands should have an equal tensile force when the last one is wedged. This is the core technology for MS iso-tensioning. In this study, a new MS cable iso-tensioning system was developed for controlling and jacking the high-strength strands, with an ultimate tensile strength of 2,200MPa, for a stay cable of extra-dosed/cable-stayed bridges. The newly developed iso-tensioning system consists of electrical resistance load cells, hydraulic jacking devices, hydraulic pumps, and integrated controllers. Moreover, it is embedded with an algorithm that can control and predict the variations in tensile forces of the Master and Slave strands in real time. Actual experiments were carried out to verify the function and performance of the newly developed system. This system was applied successfully to the stay cable construction of 2nd Tae-in extra-dosed bridge in Gwangyang.

• Research in Plant Disease
• /
• v.11 no.2
• /
• pp.98-105
• /
• 2005

All viruses have few genes relative to their hosts. Viruses, thus, utilize many host factors for efficient viral replication in host cell. Virus-host interactions are crucial determinations of host range, replication, and pathology. Host factors participate in most steps of positive-strand RNA virus infection, including entry, viral gene expression, virion assembly, and release. Recent data show that host factors play important roles in assembling the viral RNA replication complex, selecting and recruiting viral RNA replication templates, activating the viral complex for RNA synthesis, and the other steps. These virus-host interactions may contribute to the host specificity and/or pathology. Positive-strand RNA viruses encompass over two-thirds of all virus genera and include numerous pathogens. This review focuses on the importance of host factors involved in positive strand plant RNA virus genome replication.

• Journal of the Korea Furniture Society
• /
• v.11 no.2
• /
• pp.73-78
• /
• 2000

This study was carried out to measure formaldehyde emission with the passing of two years from plywood, sliver-board and strand-board bonded with urea resins which were made of 6 f/U molar ratios. The urea resins were manufactured by six kinds of formaldehyde/urea molar ratio of 1.0, 1.2, 1.4, 1.6, 1.8 and 2.0. 1. The plywood with molar ratio of 1.0 satisfied the KS F3101 $F_2$ directly after manufacture. The plywood with molar ratio of 1.2 satisfied m 3 days. The plywood with molar ratio of 1.4 satisfied the $F_3$ in 3 days and the $F_2$ in 600 days. And the plywood with molar ratio of 1.8 and 2.0 satisfied the $F_3$ in 365 days, but didn't satisfy the $F_2$ in 730 days. 2. Sliver-board with molar ratio of 1.0 and 1.2 satisfied the KS F3104 $E_2$ right after manufacture. Sliver-board with molar ratio of 1.4 and 1.6 satisfied in 150 and 360 days, respectively. Sliver-board with molar ratio of 1.8 and 2.0 satisfied in 730 days. 3. Strand-board with molar ratio of 1.0 and 1.2 satisfied the KS F3104$ E_2$ directly after manufacture. Strand-board with molar ratio of 1.4 and 1.6 satisfied in 150 days. But Strand-board with molar ratio of 1.8 and 2.0 didn't satisfied in 730 days.

• Journal of the Korea Furniture Society
• /
• v.11 no.2
• /
• pp.45-53
• /
• 2000

This study was carried out to determine the suitability of 3 months old bamboo species of Phyllostachys bambusoides S. et Z., Phyllostachys Pubescens Mazel and Phyllostachys nigra var henonis Stapf as raw materials for the manufacture of strandboard. Total of 108 strandboards were made using urea-formaldehyde resin content level of 12% and one percent of liquid wax emulsion. The strandboard consisted of three layers the top and the bottom layer of which were oriented to the same direction and weighted 25% of the strandboard each. The middle core layer weighed 50% of the board and was perpendicular to the outer top and bottom layers. Analysis was performed to determine the effect of strand lengths and Uowing years of 3 months, 2 years and 3 years on strandboard properties. The physical and mechanical properties of bamboo species and boards were measured and compared to the standard requirements of strandboards. The results are as follows; 1. The more the growing years the higher the density of bamboo. Top part of bamboo indicated higher density value than that of bottom part. 2. Bamboo showed higher static bending strength compared to the main wood species. Longer growing years of bamboo generally inclosed the static tending strength out there were no statistical significancies for Phyllostachys bmbusoides S. et Z. and Phyllostachys pubescens Mazel. 3. Strand length indicated no difference on density and moisture content of strandboard. 5 cm of strand length gave the best static bending strength and internal bonding strength. Bamboo strandboard exhibited lesser extents of thickness swelling than that of CSA standard. 4. 3 months old bamboo gave higher static bending strength of strandboard than those of 2 years and 3 years old bamboo. In case of Phyllostachys nigra var henonis Stapf, 3 months old bamboo indicated higher internal bonding strength than those of 2 years and 3years old bamboo. but in Phyllostachys bambusoides S. et Z., Phyllostachys pubescens Mazel, there were no difference among growing years. Growing years showed no different physical properties of bamboo strandboard.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.25 no.2
• /
• pp.163-173
• /
• 2012

This study covers the nonlinear analysis of anchor head for high strength prestressing strand and presents necessary process in improving the performance of anchor head. The surface of wedge for strand is contacted to the surface of the wedge hole on anchor head when it is fitted into the wedge hole, and the contact condition changes according to the level of load applied through the wedge. In order to analyze detailed behavior, nonlinear material model and contact element were used in analysis. It was found from the analysis that the behavior of anchor head is affected by the interaction with the wedge contacted so that the wedge in FE model should have the same figure as the actual object. Circular array of wedge hole presents better stress distribution than layer array even though the small difference in maximum deformation. Increment of thickness of anchor head and distance of wedge hole also improve the performance of anchor head.

• Bulletin of the Korean Chemical Society
• /
• v.28 no.9
• /
• pp.1510-1514
• /
• 2007

Recently, it has been reported that the inhibition of the strand transfer function of HIV-1 integrase is necessary to obtain significant antiviral activity. Accordingly, several compounds typified by aryl 1,3-diketo acids that can inhibit strand transfer reaction of HIV-1 IN have been identified. In this work, we synthesized new 4- hydroxy-5-azacoumarin-3-carbox(thio)amides (1a-h) and evaluated for the inhibition of HIV-1 IN strand transfer reaction with a brief SAR. Among synthesized, compound 1e was the most potent HIV-1 IN inhibitor with equipotent activity to that of L-708,906. Therefore, the 4-hydroxy-5-azacoumarin ring can be considered as a new scaffold in designing more potent of HIV-1 IN inhibitors for treatment of AIDS.

• Bulletin of the Korean Chemical Society
• /
• v.27 no.9
• /
• pp.1346-1352
• /
• 2006

We describe an effective method of microchip electrophoresis (ME) based on single strand conformation poly-morphism (SSCP) analysis to rapidly detect the point mutation, Leu72Met, in a human obesity gene. The 207-bp dsDNA in the Leu72Met region, an estimate of the child obesity DNA mutant, was amplified by polymerase chain reaction (PCR) and submitted to a conventional glass microchip analysis with a sieving matrix of 1.75% poly(vinylpyrrolidone) (Mr 1 300 000), 1.0% poly(ethyleneoxide) (Mr 600 000) and 5.0% w/w glycerol. When combined with base stacking (BS) with hydroxide ions, the SSCP-ME provided rapid analysis as well as sensitive detection. The detection sensitivity was effectively enhanced in the OH- concentration range of 0.01-0.025 M NaOH. The sensitivity and speed of this ME-based SSCP methodology for the rapid detection of Leu72Met point mutations makes this an attractive method for diagnosing childhood obesity in a clinical diagnostic laboratory.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.3
• /
• pp.469-475
• /
• 2020

During meiosis I, programmed DNA double-strand breaks (DSBs) occur to promote chromosome pairing and recombination between homologs. In Saccharomyces cerevisiae, Mec1 and Tel1, the orthologs of human ATR and ATM, respectively, regulate events upstream of the cell cycle checkpoint to initiate DNA repair. Tel1^ATM and Mec1^ATR are required for phosphorylating various meiotic proteins during recombination. This study aimed to investigate the role of Tel1^ATM and Mec1^ATR in meiotic prophase via physical analysis of recombination. Tel1^ATM cooperated with Mec1^ATR to mediate DSB-to-single end invasion transition, but negatively regulated DSB formation. Furthermore, Mec1^ATR was required for the formation of interhomolog joint molecules from early prophase, thus establishing a recombination partner choice. Moreover, Mec1^ATR specifically promoted crossover-fated DSB repair. Together, these results suggest that Tel1^ATM and Mec1^ATR function redundantly or independently in all post-DSB stages.

• Journal of Microbiology
• /
• v.37 no.3
• /
• pp.154-158
• /
• 1999

The arginine residue at position 243 (Arg 243) of the yeast transcription factor, Gcn4p, is invariably conserved among bZIP transcription factors. Using site-directed oligonucleotide saturation mutagenesis involving two-step polymerase chain reaction (PCR) amplification, random mutations were successfully introduced at the codon of 243 in the basic domain of Gcn4p. This mutant library was transformed ito Gcn4p defective yeast strain and selected for the transcriptionally active colonies. All colonies which were transcriptionally active had arginines in the codon 243. In this study, the strand preference by Taq polymerase during mutagenesis was also tested. Oligonucleotides were specially designed to test whether or not the polymerase was preferred using the strand as a template. A population of randomly mutated products were cloned into an appropriate vector and characterized by DNA sequencing analysis. Saturation mutagenesis which was performed efficiently by this method revealed a strong bias in terms of strand preference of Taq polymerase by an approximate ratio of 3 to 1 in this study.