• Coupled systems mechanics
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• v.11 no.6
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• pp.485-504
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• 2022

This paper presents nonlinear oscillations of a carbon nanotube reinforced composite beam subjected to lateral harmonic load with damping effect based on the modified couple stress theory. As reinforcing phase, three different types of single walled carbon nanotubes distribution are considered through the thickness in polymeric matrix. The non-linear strain-displacement relationship is considered in the von Kármán nonlinearity. The governing nonlinear dynamic equation is derived with using of Hamilton's principle.The Galerkin's decomposition technique is utilized to discretize the governing nonlinear partial differential equation to nonlinear ordinary differential equation and then is solved by using of multiple time scale method. The frequency response equation and the forced vibration response of the system are obtained. Effects of patterns of reinforcement, volume fraction, excitation force and the length scale parameter on the nonlinear responses of the carbon nanotube reinforced composite beam are investigated.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1630-1636
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• 2010

During the fermentation process of Saccharomyces cerevisiae, yeast cells must rapidly respond to a wide variety of external stresses in order to survive the constantly changing environment, including ethanol stress. The accumulation of ethanol can severely inhibit cell growth activity and productivity. Thus, the response to changing ethanol concentrations is one of the most important stress reactions in S. cerevisiae and worthy of thorough investigation. Therefore, this study examined the relationship between ethanol tolerance in S. cerevisiae and a unique protein called alcohol sensitive RING/PHD finger 1 protein (Asr1p). A real-time PCR showed that upon exposure to 8% ethanol, the expression of Asr1 was continuously enhanced, reaching a peak 2 h after stimulation. This result was confirmed by monitoring the fluorescence levels using a strain with a green fluorescent protein tagged to the C-terminal of Asr1p. The fluorescent microscopy also revealed a change in the subcellular localization before and after stimulation. Furthermore, the disruption of the Asr1 gene resulted in hypersensitivity on the medium containing ethanol, when compared with the wild-type strain. Thus, when taken together, the present results suggest that Asr1 is involved in the response to ethanol stress in the yeast S. cerevisiae.

• Journal of the Korean GEO-environmental Society
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• v.11 no.6
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• pp.5-20
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• 2010

One-dimensional equivalent linear site response analysis is widely used in practice due to its simplicity, requiring only few input parameters, and low computational cost. The main limitation of the procedure is that it is essentially a linear method, in which the time dependent change in the soil properties cannot be modeled and constant values of shear modulus and damping is used throughout the duration of the analysis. Various forms of modified equivalent linear analyses have been developed to enhance the accuracy of the equivalent linear method by incorporating the dependence of the shear strain with the loading frequency. The methods are identical in that it uses the shear strain Fourier spectrum as the backbone of the analysis, but differ in the method in which the strain Fourier spectrum is smoothed. This study used two domestically measured soil profiles to perform a series of nonlinear, equivalent linear, and modified equivalent linear site response analyses to verify the accuracy of two modified procedures. The results of the analyses indicate that the modified equivalent linear analysis can highly overestimate the amplification of the high frequency components of the ground motion. The degree of overestimation is dependent on the characteristics of the input ground motion. Use of a motion rich in high frequency contents can result in unrealistic response.

• Advances in aircraft and spacecraft science
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• v.9 no.3
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• pp.217-242
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• 2022

This paper proposes a novel time-domain homogenization model combining the viscoelastic constitutive law with Eshelby's inclusion theory-based micromechanics model to predict the mechanical behavior of the particle reinforced composite material. The proposed model is intuitive and straightforward capable of predicting composites' viscoelastic behavior in the time domain. The isotropization technique for non-uniform stress-strain fields and incremental Mori-Tanaka schemes for high volume fraction are adopted in this study. Effects of the imperfectly bonded interphase layer on the viscoelastic behavior on the dynamic mechanical behavior are also investigated. The proposed model is verified by the direct numerical simulation and DMA (dynamic mechanical analysis) experimental results. The proposed model is useful for multiscale analysis of viscoelastic composite materials, and it can also be extended to predict the nonlinear viscoelastic response of composite materials.

• Journal of Microbiology and Biotechnology
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• v.20 no.9
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• pp.1314-1321
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• 2010

Sulfur metabolism in S. cerevisiae is well established, but the mechanisms underlying the formation of sulfide remain obscure. Here, we investigated by real-time RT-PCR the dependence of expression levels of MET3, MET5/ECM17, MET10, MET16, and MET17 along with SSU1 on nitrogen availability in two wine yeast strains that produce divergent sulfide profiles. MET3 was the most highly expressed of the genes studied in strain PYCC4072, and SSU1 in strain UCD522. The strains behaved differently according to the sampling times, with UCD522 and PYCC4072 showing the highest expression levels at 120 h and 72 h, respectively. In the presence of 267 mg assimilable N/l, the genes were more highly expressed in strain UCD522 than in PYCC4072. MET5/ECM17 and MET17 were only weakly expressed in both strains under any condition tested. MET10 and SSU1 in both strains, but MET16 only in PYCC4072, were consistently upregulated when sulfide production was inhibited. This study illustrates that strain genotype could be important in determining enzyme activities and therefore the rate of sulfide liberation. This linkage, for some yeast strains, of sulfide production to expression levels of genes associated with sulfate assimilation and sulfur amino acid biosynthesis could be relevant for defining new strategies for the genetic improvement of wine yeasts.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.4
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• pp.721-725
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• 2007

A fiber optic based weight sensor has fabricated using a fiber Bragg grating with a weight sensitive. The sensing concept exploits the inherent characteristics of the FBG and is based on the strain effect induced in the fiber Bragg grating through. A direct indication of the weight level is given by the shift of the Bragg wavelength caused by the expansion of the sensing material. A FBG behaves like a spectral filter which has inherent characteristics that render it very sensitive to strain and temperature. The sensing principle is also based on the strain effect induced in the FBG through the caused by the weight. The experimental setup used for the initial investigation to characterize the mass response of the sensor. The transmitted signal from the sensor was monitored using an optical spectrum analyzer with a resolution bandwidth of 0.4nm. In this paper, we presented the spectral characterization and shaping of FBG by scanning a mass element that affects a small grating fraction at a time, without permanent effects on the optical fiber when the various wavelength and strain is removed. That is, destruction when the optical fiber for weight is physically damaged.

• Advances in nano research
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• v.7 no.4
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• pp.249-263
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• 2019

In the current paper, an exact solution method is carried out for analyzing the thermo-mechanical vibration of curved FG nano-beams subjected to uniform thermal environmental conditions, by considering porosity distribution via nonlocal strain gradient beam theory for the first time. Nonlocal strain gradient elasticity theory is adopted to consider the size effects in which the stress for not only the nonlocal stress field but also the strain gradients stress field is considered. It is perceived that during manufacturing of functionally graded materials (FGMs) porosities and micro-voids can be occurred inside the material. Material properties of curved porous FG nanobeam are assumed to be temperature-dependent and are supposed to vary through the thickness direction of beam which modeled via modified power-law rule. Since variation of pores along the thickness direction influences the mechanical and physical properties, porosity play a key role in the mechanical response of curved FG nano-structures. The governing equations and related boundary condition of curved porous FG nanobeam under temperature field are derived via the energy method based on Timoshenko beam theory. An analytical Navier solution procedure is utilized to achieve the natural frequencies of porous FG curved nanobeam supposed to thermal loading. The results for simpler states are confirmed with known data in the literature. The effects of various parameters such as nonlocality parameter, porosity volume fractions, thermal effect, gradient index, opening angle and aspect ratio on the natural frequency of curved FG porous nanobeam are successfully discussed. It is concluded that these parameters play key roles on the dynamic behavior of porous FG curved nanobeam. Presented numerical results can serve as benchmarks for future analyses of curve FG nanobeam with porosity phases.

• Journal of the Korean Geotechnical Society
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• v.20 no.5
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• pp.145-159
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• 2004

The conventional method for assessment of liquefaction potential proposed by Seed and Idriss has been widely used in most countries because of simplicity of tests. Even though various data such as stress, strain, stress path, and excess pore water pressure can be obtained from the dynamic test, especially, two simple experimental data such as the maximum deviatoric stress and the number of cycles at liquefaction have been used in the conventional assessment. In this study, a new detailed assessment for liquefaction potential to reflect both characteristics of real earthquake motion and dynamic soil resistance is proposed and verified. In the assessment, the safety factor of the liquefaction potential at a given depth of a site can be obtained by the ratio of a resistible cumulative plastic shear strain determined through the performance of the conventional cyclic test and a driving cumulative plastic shear strain calculated from the shear strain time history through the ground response analysis. The last point to cumulate the driving plastic shear strain to initiate soil liquefaction is important for this assessment. From the result of cyclic triaxial test using real earthquake motions, it was concluded that liquefaction under the impact-type earthquake loads would initiate as soon as a peak loading signal was reached. The driving cumulative plastic shear strain, therefore, can be determined by adding all plastic shear strains obtained from the ground response analysis up to the peak point. Through the verification of the proposed assessment, it can be concluded that the proposed assessment for liquefaction potential can be a progressive method to reflect both characteristics of the unique soil resistance and earthquake parameters such as peak earthquake signal, significant duration time, earthquake loading type, and magnitude.

• Journal of the Society of Naval Architects of Korea
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• v.45 no.6
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• pp.726-734
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• 2008

While the structural safety assessment of Cargo Containment System(CCS) in membrane type LNG carriers has to be carried out in consideration of sloshing impact pressure, it is very difficult to figure out its dynamic response behaviors due to its very complex structural arrangements/materials and complicated phenomena of sloshing impact loading. For the development of its original technique, it is necessary to understand the characteristics of dynamic response behavior of CCS structure under sloshing impact pressure. In this study, for the exact understanding of dynamic response behavior of CCS structure in membrane Mark III type LNG carriers under sloshing impact pressure, its wet drop impact response analyses were carried out by using Fluid-Structure Interaction(FSI) analysis technique of LS-DYNA code, and were also validated through a series of wet drop experiments for the enhancement of more accurate shock response analysis technique. It might be thought that the structural response behaviors of impact response analysis, such as impact pressure impulses and resulted strain time histories, generally showed very good agreement with experimental ones with very appropriate use of FSI analysis technique of LS-DYNA code, finite element modeling and material properties of CCS structure, finite element modeling and equation of state(EOS) of fluid domain.

• Research in Plant Disease
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• v.30 no.2
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• pp.148-156
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• 2024

Ralstonia pseudosolanacearum, a plant pathogenic bacterium that can survive for a long time in soil and water, causes lethal wilt in the Solanaceae family. Sigma S is a part of the RNA polymerase complex, which regulates gene expression during bacterial stress response or stationary phase. In this study, we investigated the role of sigma S in R. pseudosolanacearum under stress conditions using a rpoS-defective mutant strain of R. pseudosolanacearum and its wild-type strain. The phenotypes of rpoS-defective mutant were complemented by introducing the original rpoS gene. There were no differences observed in bacterial growth rate and exopolysaccharide production between the wild-type strain and the rpoS mutant. However, the wild-type strain responded more sensitively to nutrient deficiency compared to the mutant strain. Under the nutrient deficiency, the rpoS mutant maintained a high bacterial viability for a longer period, while the viability of the wild-type strain declined rapidly. Furthermore, a significant difference in pH was observed between the culture supernatant of the wild-type strain and the mutant strain. The pH of the culture supernatant for the wild-type strain decreased rapidly during bacterial growth, leading to medium acidification. The rapid decline in the wild-type strain's viability may be associated with medium acidification and bacterial sensitivity to acidity during transition to the stationary phase. Interestingly, the rpoS mutant strain cannot utilize acetic acid, D-alanine, D-trehalose, and L-histidine. These results suggest that sigma S of R. pseudosolanacearum regulates the production or utilization of organic acids and controls cell death during stationary phase under nutrient deficiency.