• Title/Summary/Keyword: strain improvement

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Experimental Study on Improvement of Pipe-rack Joint (Pipe-rack접합부 개선방법에 관한 실험적 연구)

  • Lee, Jong-Kun
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.22 no.4
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    • pp.1-9
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    • 2018
  • The development of new technology and process in industrial Plant which builds integrated structures, facilities and systems. Has become a key element for strengthening its competitiveness. Although domestic industrial Plant has demonstrated excellence in technology with a persistent increase in order quantity and orders received, the technology gap between countries has narrowed due to global construction trend. Therefore, it is necessary to develop new technology that could help overcome constraints and limitations of the current one to follow the trend in the age of unlimited competition. This study has focused on assembly technology of Pipe-rack joint connection in an effort to strengthen technological competitiveness in industrial Plant. Through an analysis of earlier studies on Pipe-rack and a coMParative analysis of strengths and weaknesses of current assembly technology of it, a new design plan has been made to improve it efficiently. In doing this, standards for design factors of both structural and performance features have been drawn, and value of stress, strain, moment and rotation has been calculated using finite element analysis. As a result, installation technology of modular type Pipe-rack, which has not been developed in Korea and is differentiated from the current one, has been developed. It is considered that the technology reduces work time and saves cost due to simplified joint connection of steel structure, unlike the current one. Moreover, since it is installed without a welding process in the field, industrial accidents would be reduced, which is likely to have economic competitiveness and satisfy.

A Study on the Ground Improvement Effect with Grouting in Backside of Retaining Wall (흙막이 벽체 배면 그라우팅 시 지반보강 효과에 관한 연구)

  • Chu, Ickchan;Byun, Yoseph;Baek, Seungin;Chun, Byungsik
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.10
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    • pp.77-83
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    • 2012
  • Recently, excavations using propped walls were popularized in downtown due to reduced settlement of nearby structures. These excavations is induced strain to propped walls or settlement in near ground. In this study, the ground reinforcing effect was proven using NDS, which is an inorganic injection material. Injection tests were performed to compute optimum injection pressure and volume. Next, calibration chamber tests were performed by using computed injection pressure and volume, and wall behaviour was examined for overburden pressures of 50kPa and 150kPa. Ground reinforcing effect was shown when the material behind the propped wall was grouted. From test results, optimum injection pressure was 350kPa and the optimum volume was 10L considering economics. Calibration chamber test results show that after the material was grouted, the maximum settlement was reduced to 19% of the non-grouted condition. For overburden pressures of 50kPa and 150kPa behind the wall, the settlement of the wall increased by 58% and 57% when compared to the case of no overburden pressure.

Comparison and Validation of Genetic Diversity and Population Structure Using Monomorphic SNP Data of the Korean Native Black Goat and Crossbred Goat (재래흑염소와 교잡종 염소의 Monomorphic SNP 분석을 통한 유전적 다양성과 집단구조의 비교 및 검증)

  • Kim, Kwan-Woo;Lee, Jinwook;Lee, Eun-Do;Lee, Sung-Soo;Choi, You-Lim;Lim, Hyun-Tae;Kim, Yousam;Lee, Sang-Hoon
    • Journal of Life Science
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    • v.30 no.11
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    • pp.1007-1011
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    • 2020
  • This study was conducted to analyze the genetic diversity and relationships that discriminate between Korean native black goat populations (Dangjin, Jangsu, Tongyoung, and Gyeongsang National University strains) and crossbred goats. Monomorphic single nucleotide polymorphisms (SNPs) in each strain were collected, and 133 common SNPs were selected for analysis. These 133 monomorphic SNPs showed differences in the genetic structure of the Korean native black goat and crossbred goats, and results from the principal component analysis (PCA) showed that the two can be clearly separated. Furthermore, analysis of the validation population comprising 70 individuals (Korean native black goats, n = 24; crossbred goats, n = 46) with the reference population showed that Korean native black goat strains and the reference population have the same genetic structure, and the crossbred goats shared only part of the genetic structure with the reference population. The result of the PCA analysis showed that the Korean native black goat strains form one population, whereas the foreign strains form another population which is more widely dispersed than the Korean native black goat strains. Thus, the results from this study can be used as baseline data for the conservation of genetic resources of Korean native black goat communities through utilization of monomorphic SNPs and for the introduction of exotic species for further improvement in genetic diversity. This study can also help reduce unnecessary inbreeding and gene flow between native strains.

Media Optimization of Corynebacterium glutamicum for Succinate Production Under Oxygen-Deprived Condition

  • Jeon, Jong-Min;Thangamani, Rajesh;Song, Eunjung;Lee, Hyuk-Won;Lee, Hong-Weon;Yang, Yung-Hun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.2
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    • pp.211-217
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    • 2013
  • Corynebacterium glutamicum is one of the well-studied industrial strain that is used for the production of nucleotides and amino acids. Recently, it has also been studied as a possible producer of organic acids such as succinic acid, based on its ability to produce organic acids under an oxygen deprivation condition. In this study, we conducted the optimization of medium components for improved succinate production from C. glutamicum under an oxygen deprivation condition by Plackett-Burman design and applied a response surface methodology. A Plackett-Burman design for ten factors such as glucose, ammonium sulfate, magnesium sulfate, potassium phosphate ($K_2HPO_4$ and $KH_2PO_4$), iron sulfate, manganese sulfate, biotin, thiamine, and sodium bicarbonate was applied to evaluate the effects on succinate production. Glucose, ammonium sulfate, magnesium sulfate, and dipotassium phosphate were found to have significant influence on succinate production, and the optimal concentrations of these four factors were sequentially investigated by the response surface methodology using a Box-Behnken design. The optimal medium components obtained for achieving maximum concentration of succinic acid were as follows: glucose 10 g/l, magnesium sulfate 0.5 g/l, dipotassium phosphate ($K_2HPO_4$) 0.75 g/l, potassium dihydrogen phosphate ($KH_2PO_4$) 0.5 g/l, iron sulfate 6 mg/l, manganese sulfate 4.2 mg/l, biotin 0.2 mg/l, thiamine 0.2 mg/l, and sodium bicarbonate 100 mM. The parameters that differed from a normal BT medium were glucose changed from 40 g/l to 10 g/l, dipotassium phosphate ($K_2HPO_4$) 0.5 g/l changed to 0.75 g/l, and ammonium sulfate ($(NH_4)_2SO_4$) 7 g/l changed to 0 g/l. Under these conditions, the final succinic acid concentration was 16.3 mM, which is about 1.46 fold higher than the original medium (11.1 mM) at 24 h. This work showed the improvement of succinate production by a simple change of media components deduced from sequential optimization.

A STUDY ON THE EFFECT OF THERMOCYCLING TO THE PHYSICAL PROPERTIES OF DENTURE LINERS (열 순환에 따른 의치이장채의 물리적 성질의 변화에 관한 연구)

  • Lee Dong-Su;Lim Heon-Song;Lim Ju-Hwan;Cho In-Ho
    • The Journal of Korean Academy of Prosthodontics
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    • v.39 no.5
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    • pp.556-575
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    • 2001
  • For the improvement of denture fitness of changed residual ridge, denture liner can be used. Denture liner should be very stable physically in various environments of the mouth as well as be bonded strongly with denture resin. In this study. the specimens bonded with four kinds of soft denture liner and three kinds of hard denture liner were used to test the physical properties of the liners. All experimental groups were stored in $37{\pm}1^{\circ}C$ distilled water for 24hours, followed by thermocycling between $15^{\circ}C$ and $45^{\circ}C$ with 15 second dwell time. 1000, 2000, 3000 cycles of thermocycling were excuted and physical properties were measured by Instron Universal Testing Machine. The obtained results were as follows : 1. In tensile bond test of sea liners, it was shown that both of $Molloplast-B^{(R)}$ specimens before and after thermocycling had the highest tensile strength, and in case of hard liners, Dura-Liner $II^{(R)}$ specimen had the highest tensile strength before and after thermocycling. Depending on thermocycling, $Soft-Relining^{(R)}$, $Denture-Relining^{(R)}$, $Molloplast-B^{(R)}$, $Coe-Soft^{(R)}$ and $Kooliner^{(R)}$ specimen showed significant difference(p<0.05). 2. In strain test of soft liners, it was shown that $Molloplast-B^{(R)}$ specimen before thermocycling and the $Coe-Soft^{(R)}$ after thermocycling showed highest results, and in case of hard liners, the Dura-Liner $II^{(R)}$ specimen before and after thermocycling had the highest result. Depending on thormocycling, $Denture-Relining^{(R)}$, $Molloplast-B^{(R)}$ and Dura-Liner $II^{(R)}$ specimen showed significant difference(p<0.05). 3. In maximum distance test of soft liners. the $Molloplast-B^{(R)}$ specimen before thermocycling and the $Coe-Soft^{(R)}$ after thermocycling showed highest results. and in case of hard liners, the Dura-Liner $II^{(R)}$ specimen before and after thermocycling showed highest result. Depending on thermocycling, $Denture-Relining^{(R)}$, $Molloplast-B^{(R)}$ specimens showed significant difference(p<0.05). 4. In elasticity test of soft liners, the $Molloplast-B^{(R)}$ specimen before and after thermocycling showed highest result. and in case of hard liners, the Dura-Liner $II^{(R)}$ specimen before thermocycling and the $Tokuso-Rebase^{(R)}$ after thermocycling showed highest results. Depending on thermocycling, $Soft-Relining^{(R)}$ $Molloplast-B^{(R)}$ specimens showed significant difference (p<0.05).

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Evaluation on Stress-Strain-Strength Behavior of the Textile Encased Soils via Triaxial Compression Tests (삼축압축시험을 통한 섬유로 구속된 흙의 응력-변형률-강도 거동 평가)

  • Yoo, Wan-Kyu;Kim, Byoung-Il;Cho, Wanjei
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.2
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    • pp.643-653
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    • 2013
  • Recently, there are an increasing number of studies on the method of wrapping the outer wall of granular piles with geosynthetic fibers such as geotextile or geogrid that has a certain level of tensile strength as an alternative method for the ground improvement techniques. In this study, triaxial compression tests are performed on the sand and clay specimen encased with various textiles to evaluate the reinforcing effect with regard to the tensile strength of the textile. Furthermore, triaxial compression tests are performed on the clay specimen inserted by sand only and sand encased with geosynthetics to compare behavioral differences between the conventional sand compaction pile and geosynthetic encased sand pile with regard to the replacement ratio, ${\alpha}_s$ and the tensile strength of the geosynthetics. Based on the experimental results, the strength enhancement due to the textile is affected by the longitudinal tensile strength rather than the transverse one of the applied textile. The effect of the confinement by the textile encasement results in the large increase of the cohesions. The overall behaviors, such as shear strength, pore pressure parameter at failure and stress ratio, of the geosynthetic encased sand pile is quite different from those of the conventional sand compaction pile.

A Study on the Improvement of Dietary Protein-efficiency by Supplement of the Panax Ginseng-by-products. (인삼의 부산물을 이용한 식의성 단백질의 효율 향상을 위한 연구)

  • 황우익;이성동
    • Journal of Ginseng Research
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    • v.3 no.1
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    • pp.1-34
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    • 1979
  • Our nation is confronted with the situation that the rice, a principal food, short of some essential amino acids, leads to imbalanced meals insufficient in the nutrient of Protein, to bring many difficulties in the elevation of nutritional state in our nation. While. our country has been produced much amounts of Panax Ginseng roots which has a stimulating effects on the metabolism of protein, lipid and nucleic acids in the body. And the leaf and trunk of Panax Ginseng were also produced a considerable amounts as the by-products. Author believe that these by-products (leaf and trunk) of Panax Ginseng might have some components possessing simillar activity with Panax Ginseng root although the quantity and qualify of the functional components may more or less be different. Therefore, this study was demised to observe the supplemental effect of the Panax Ginseng-by-Products on the dietary protein efficiency and nutritional state of rats. The feeds used for this experiment were rice containing 30% barely, fish four, and the leaf, trunk and small root of the Panax Ginseng, and the contents of the general nutrients including protein, lipid and carbohydrate etc. in each feed were analyzed for the combination of each feed. And, being based on analytical values of Protein in food. fish Pour as Protein source was added were rice containing 30% barely to be include 8.6 to 8.7%, 12%, 15% and 18% of protein. Then 2% of the leaf, trunk or small reef of Panax Ginseng was supplemented into each of above protein diet group, ton 16 kinds of diets were Prepared. The male albino rats from a Pure strain, weighing 70g to 80g. were used for experimental animals. They were maintained with coresponding fist for f and 8 weeks, and the growth rate, consumption of diets and protein, efficiency of feed and Protein in animals were determined. The lipids, proteins and cholesterols in serum and liver were also determined quantitatively after they were sacrificed in coresponding term. The results obtained are summarized as follows: 1. Body weigh of diet group containing 8.6 to 8.7%,12%, and 15% of protein are increased remarkably by supplement of 2% of the leaf or small root of Panax Ginseng in comparison with each of controls. But this tendency could not observed in diet group containing 18eA Proteins. 2. Feed efficiency showed same tendency in comparison with changes of gained body weight. Specially, in each of diets containing 8.7%, 12%, 15% and 18% of Proteins, supplement of the leaf of Panax Ginseng showed the better feed efficiency than supplement of the trunk or small root. 3. In feeding group for 8 weeks, protein efficiency showed worst efficiency in diet containing 18% proteins and showed the best efficiency was the diet group containing 12% Proteins. And the efficiency was improved according to supplement of the leaf of Panax Ginseng. 4. Nitrogen contents in serum and liver did not show large differences each other in all diet groups. But contexts of total cholesterol and 1ipid were decreased markedly in diet groups containing 12%, 15% and 18% of proteins in comparison with diet group containing 8.6% to 8.8% of proteins.

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Effect of Supplementation with Docosahexaenoic Acid from Gestation to Adulthood on Spatial Learning Performance in Rat (임신기부터 성장기 동안 Docosahexaenoic Acid 보충에 의한 흰쥐의 공간기억력 개선 효과)

  • Lim, Sun-Young
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1400-1405
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    • 2007
  • The effect of supplementation with docosahexaenoic acid into n-3 fatty acid deficient diet on improvement of loaming related brain function was investigated. On the second day after conception, Sprague Dawley strain dams were subjected to a diet containing either n-3 fatty acid deficient (Def) or n-3 fatty acid deficient + docosahexaenoic acid (Def+DHA). After weaning, male pups were fed on the same diet of their respective dams until adulthood. Motor activity and Morris water maze tests were measured at 10 weeks old. In motor activity test, there were no statistically significant differences in moving time and moving distance between the Def and Def+DHA diet groups. The n-3 fatty acid deficient with DHA (Def+DHA) group exhibited a shorter escape latency, swimming time and swimming distance (P<0.05) compared to the n-3 fatty acid deficient group (Def) but there was no difference in resting time and swimming speed between the experimental diet groups. In memory retention trial, the number of crossing of the platform position (region A) was significantly greater than those of other regions for the Def+DHA group. However, the Def group swam randomly without preference for the provisions platform location, indicating poorer memory retention. From those results, supplementation with DHA into the n-3 fatty acid deficient diet improved the spatial loaming ability in rats as assessed by Morris water maze test.

Effect of Treatment with Docosahexaenoic Acid into N-3 Fatty Acid Adequate Diet on Learning Related Brain Function in Rat (N-3계 지방산 적절 함량 식이의 docosahexaenoic acid 첨가가 기억력 관련 뇌 기능에 미치는 영향)

  • Lim, Sun-Young
    • Journal of Life Science
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    • v.19 no.7
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    • pp.917-922
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    • 2009
  • The effect of adding docosahexaenoic acid into an n-3 fatty acid adequate diet on the improvement of learning related brain function was investigated. On the second day after conception, Sprague Dawley strain dams were subjected to a diet containing either n-3 fatty acid adequate (Adq, 3.4% linolenic acid) or n-3 fatty acid adequate+docosahexaenoic acid (Adq+DHA, 3.31%linolenic acid plus 9.65% DHA). After weaning, male pups were fed on the same diet of their respective dams until adulthood. Motor activity and Morris water maze tests were measured at 10 weeks. In the motor activity test, there were no statistically significant differences in moving time and moving distance between the Adq and Adq+DHA diet groups. The n-3 fatty acid adequate with DHA (Adq+DHA) group tended to show a shorter escape latency, swimming time and swimming distance compared to the n-3 fatty acid adequate group (Adq), but the differences were not statistically significant. There was no difference in resting time, but the Adq+DHA group showed a higher swimming speed compared to the Adq group. In memory retention trials, the numbers of crossing of the platform position (region A), in which the hidden platform was placed, were significantly greater than those of other regions for both Adq and Adq+DHA groups. Based on these results, adding DHA into the n-3 fatty acid adequate diet from gestation to adulthood tended to induce better spatial learning performance in Sprague Dawley rats as assessed by the Morris water maze test, although the difference was not significant.

Physiological and Ecological Characteristics of Lipid-Producing Botryococcus Isolated from the Korean Freshwaters (한국산 고지질 미세조류 Botryococcus의 분포 및 생장 특성)

  • Shin, Sang-Yoon;Jo, Beom-Ho;Lee, Hyung-Gwan;Oh, Hee-Mock
    • Korean Journal of Environmental Biology
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    • v.31 no.4
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    • pp.288-294
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    • 2013
  • Recently, sustainable production of biofuel using algal biomass is being pursued because of its enormous potential. First and foremost, securing superior strains to develop an efficient production system for algal biodiesel through screening or genetic improvement of microalgae is necessary. The genus of Botryococcus is regarded as one of the superior microalgae for biodiesel production due to its ability to accumulate high amounts of lipids and hydrocarbons. However, its low growth rate is a bottleneck for large-scale production and commercialization. The purpose of this study is to obtain indigenous Botryococcus strains which possess high lipid content and biomass productivity. The Botryococcus sp. was isolated from the Seobu Reservoir in Jeju Island and identified as Botryococcus sudeticus J2 by comparative analysis of 18s rRNA gene and ITS regions. The biomass productivity and lipid content of B. sudeticus J2 were 0.116 g $L^{-1}day^{-1}$ and 40.1% of dry wt., respectively. This was higher than the value of B. braunii UTEX 572, which is widely regarded as a superior strain among Botryococcus species. The relatively high growth rate of B. sudeticus J2 was achieved under a light intensity of 240 ${\mu}mol$ photons $m^{-2}s^{-1}$ with ambient air spargingwhen compared to 120 ${\mu}mol$ photons $m^{-2}s^{-1}$ with 2% $CO_2$ supply. In summary, it is likely that the isolated B. sudeticus J2 can be used for the mass cultivation and biodiesel production.