• Journal of Ocean Engineering and Technology
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• v.18 no.4 s.59
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• pp.46-51
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• 2004

The surface layer in dredged and reclaimed marine clay is improved by mixing of shallow soils and hardening agents, which is made of cement, containing some other special admixtures. Tests in both laboratory and field settings are performed to investigate the improvement effect and strength properties of cement-stabilized soils. The test results show that the hardening agent sufficiently improves the soil properties of the surface layer, while increasing the load-carrying capacity. The strength of cement-stabilized soils depends, primarily, on water-to-cement ratio and curing temperature. That is, the higher curing temperature and the longer curing time, the higher the strength in cement-stabilized soils. The high ratio of water-ta-cement results in a lower strength.

• Geomechanics and Engineering
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• v.8 no.1
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• pp.53-66
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• 2015

Plastic wastes, particularly polyethylene terephthalate (PET) generated from used bottled water constitute a worldwide environmental issue. Reusing the PET waste for geotechnical applications not only reduces environmental burdens of handling the waste, but also improves inherent engineering properties of soil. This paper investigated factors affecting shear strength improvement of PET-mixed residual soil. Four variables were considered: (i) plastic content; (ii) plastic slenderness ratio; (iii) plastic size; and (iv) soil particle size. A series of unconfined compression tests were performed to determine the optimum configurations for promoting the shear strength improvement. The results showed that the optimum slenderness ratio and PET content for shear strength improvement were 1:3 and 1.5%, respectively. Large PET pieces (i.e., $1.0cm^2$) were favorable for fine-grained residual soil, while small PET pieces (i.e., $0.5cm^2$) were favorable for coarse-grained residual soil. Higher shear strength improvement was obtained for PET-mixed coarse-grained residual soil (148%) than fine-grained residual soils (117%). The orientation of plastic pieces in soil and frictional resistance developed between soil particles and PET surface are two important factors affecting the shear strength performance of PET-mixed soil.

• Geomechanics and Engineering
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• v.12 no.4
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• pp.689-705
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• 2017

The present study deals with the Pressure-settlement behavior of square and rectangular skirted footing resting on sand and subjected to a vertical load through a laboratory experimental study. A series of load tests were conducted in the model test tank to evaluate the improvement in pressure-settlement behavior and bearing capacity of square and rectangular model footings with and without structural skirt. The footing of width 5 cm and 6 cm and length/width ratio of 1 and 2 was used. The relative density of sand was maintained at 30%, 50%, 70%, and 87% respectively. The depth of skirt was varied from 0.25 B to 1.0 B. All the tests were carried out using a strain controlled loading frame of 50 kN capacity. The strain rate for all test was kept 0.24 mm/min. The results of present study reveal that, the use of structural skirt improves the bearing capacity of footing significantly. The improvement in bearing capacity was observed almost linearly proportional to the depth of skirt. The improvement in bearing capacity of skirted footings over footing without skirt was observed in the range of 33.3% to 68.5%, 68.9% to 127% and 146.7% to 262% for a skirt depth of 0.25 B, 0.50 B and 1.0 B respectively. The skirted footings were found more effective for sand at relative density of 30% and 50% than at relative density of 70% and 87%. The bearing capacity was found to increase linearly with footing width for footings with and without skirts. This observation was found to be consistent for footings with different skirt depths and for relative density of sand i.e., 30%, 50%, 70%, and 87%. The obtained results from the study for footing with and without skirts were comparable with available solutions from literature.

• Journal of the Korean Geotechnical Society
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• v.18 no.4
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• pp.65-73
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• 2002

Underground flexible pipes for electric cables are subject to external loads and surrounding soil pressure. Particularly, strain of flexible pipes is of great concern in terms of safety and maintenance for electric cables. In this paper, stress and strain of flexible pipes with various depth are compared using traditional formula, FEM analysis and model soil box test. The results show that theoretical values are more conservative in strain in comparison with model soil box test and FEM analysis. Considering the strain criteria - maximum 3.5%, flexible pipes can be buried at the depth of 40cm without additional soil improvement. From the result of this study, deformation formula compatible with the field condition was proposed.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1093-1100
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• 2015

Acetate and lactate in growth media are detrimental to the production of Thermus maltogenic amylase (ThMA), a heterologous protein, as well as to the growth of recombinant Escherichia coli. Only 50 mM of acetate or 10 mM of lactate reduced 90% of specific ThMA activity. In this study, mutant E. coli strains blocked in the ackA-pta or ackA-pta and ldh pathways were created, characterized, and assessed for their culture performace in 300 L-scale fermentation. The ackApta and ldh double-mutant strain formed significantly less lactate and acetate, and produced a concomitant increase in the excretion of pyruvate (17.8 mM) under anaerobic conditions. The ackA-pta mutant strain accumulated significant acetate but had an approximately 2-fold increase in the formation of lactate. The ackA-pta and ldh double-mutant strain had superior overall performance in large-scale culture under suboptimal conditions, giving 67% higher cell density and 66% higher ThMA activity compared with those of the control strain. The doublemutant strain also achieved a 179% improvement in volumetric ThMA production.

• Journal of Microbiology and Biotechnology
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• v.20 no.9
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• pp.1314-1321
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• 2010

Sulfur metabolism in S. cerevisiae is well established, but the mechanisms underlying the formation of sulfide remain obscure. Here, we investigated by real-time RT-PCR the dependence of expression levels of MET3, MET5/ECM17, MET10, MET16, and MET17 along with SSU1 on nitrogen availability in two wine yeast strains that produce divergent sulfide profiles. MET3 was the most highly expressed of the genes studied in strain PYCC4072, and SSU1 in strain UCD522. The strains behaved differently according to the sampling times, with UCD522 and PYCC4072 showing the highest expression levels at 120 h and 72 h, respectively. In the presence of 267 mg assimilable N/l, the genes were more highly expressed in strain UCD522 than in PYCC4072. MET5/ECM17 and MET17 were only weakly expressed in both strains under any condition tested. MET10 and SSU1 in both strains, but MET16 only in PYCC4072, were consistently upregulated when sulfide production was inhibited. This study illustrates that strain genotype could be important in determining enzyme activities and therefore the rate of sulfide liberation. This linkage, for some yeast strains, of sulfide production to expression levels of genes associated with sulfate assimilation and sulfur amino acid biosynthesis could be relevant for defining new strategies for the genetic improvement of wine yeasts.

• Journal of the Korean GEO-environmental Society
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• v.16 no.10
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• pp.5-14
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• 2015

Suction affects the unsaturated soil as the negative pore pressure, and leads to increases of the yield stress and the plastic shear stiffness of the soil skeleton due to the growth in interparticle stress. Hence, in this study, in order to account for these effects of suction under the dynamic loading condition such as the earthquake, the element simulation of the cyclic triaxial test using induced stress-strain relation based on cyclic elasto-plastic constitutive model extended for unsaturated soil considering the $1^{st}$ and the $2^{nd}$ yield functions was conducted. Through the stress path, stress-strain relation and relation between volumetric strain and axial strain, it was seen in all the cases that the simulation results demonstrated a good agreement with the experimental results. It is expected that the results of this study possibly contribute to the accuracy improvement on the prediction of unsaturated soil behavior under the dynamic loading condition.

• The Journal of the Acoustical Society of Korea
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• v.30 no.5
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• pp.239-248
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• 2011

In order to improve the quality of strain images in medical ultrasound imaging, displacements need to be accurately estimated. In this paper, in order to apply one-dimensional displacement estimation methods to two-dimensional motion estimation, the axial and lateral displacements are separately estimated. In order to estimate lateral displacements, one-dimensional signals aligned in the lateral direction are converted to analytic signals, which are then crosscorrelated. Strain images are produced by first compensating two-dimensional displacements for lateral motion with lateral motion displacement estimates obtained from the proposed lateral displacement estimation algorithm and then estimating axial displacements. Both phantom and human data experiments show that the proposed method provides better signal-to-noise ratio and contrast-to-noise ratio characteristics than a conventional strain imaging method that utilizes axial displacement estimates only.

• Korean Journal of Organic Agriculture
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• v.20 no.3
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• pp.327-343
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• 2012

In order to develop a high cellulolytic direct-fed microorganism (DFM) for ruminant productivity improvement, this study isolated cellulolytic bacteria from the rumen of Holstein dairy cows, and compared their cellulolytic abilities via DM degradability, gas production and cellulolytic enzyme activities. Twenty six bacteria were isolated from colonies grown in Dehority's artificial (DA) medium with 2% agar and cultured in DA medium containing filter paper at $39^{\circ}C$ for 24h. 16s rDNA gene sequencing of four strains from isolated bacteria showed that H8, H20 and H25 strains identified as Ruminococcus flavefaciens, and H23 strain identified as Fibrobacter succinogenes. H20 strain had higher degradability of filter paper compared with others during the incubation. H8 (R. flavefaciens), H20 (R. flavefaciens), H23 (F. succinogenes), H25 (R. flavefaciens) and RF (R. flavefaciens sijpesteijn, ATCC 19208) were cultured in DA medium with filter paper as a single carbon source for 0, 1, 2, 3, 4 and 6 days without shaking at $39^{\circ}C$, respectively. Dry matter degradability rates of H20, H23 and H25 were relatively higher than those of H8 and RF since 2 d incubation. The cumulative gas production of isolated cellulolytic bacteria increased with incubation time. At every incubation time, the gas production was highest in H20 strain. The activities of carboxymethylcellulase (CMCase) and Avicelase in the culture supernatant were significantly higher in H20 strain compared with others at every incubation time (p<0.05). Therefore, although further researches are required, the present results suggest that H20 strain could be a candidate of DFM in animal feed due to high cellulolytic ability.

• Journal of the Optical Society of Korea
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• v.18 no.5
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• pp.523-530
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• 2014

Machine material and structural strain are critical factors for appraising mechanical properties and safety. Particularly in three and four-point bending tests, which appraise the deflection and flexural strain of an object due to external force, measurements are made by the crosshead movement or deflection meter of a universal testing machine. The Digital Image Correlation (DIC) method is one of the non-contact measurement methods. It uses the image analyzing method that compares the reference image with the deformed image for measuring the displacement and strain of the objects caused by external force. Accordingly, the advantage of this method is that the object's surface roughness, shape, and temperature have little influence. However, its disadvantage is that it requires extensive time to compare the reference image with the deformed image for measuring the displacement and strain. In this study, an algorithm is developed for DIC that can improve the speed of image analysis for measuring the deflection and strain of an object caused by a three-point bending load. To implement this algorithm for improving the speed of image analysis, LabVIEW 2010 was used. Furthermore, to evaluate the accuracy of the developed fast correlation algorithm, the deflection of an aluminum specimen under a three-point bending load was measured by using the universal test machine and DIC measurement system.