• The Korean Journal of Food And Nutrition
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• v.36 no.4
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• pp.236-243
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• 2023

The purpose of this study was to investigate the effects of sterilization, storage period and washing yuzu, according to the washing method, during the storage period. The results showed that the fungus size increased as the storage period increased, and no mold occurred in the yuzu washed with ozone water until 20 days. After 30 days of storage, a mold of 124.1±13.9 mm² was observed. The no-treatment sample had a fungus of 814.5±72.8 mm² in size on day 0 and the fungus the largest fungus was 6,362±636.7 mm² on day 30. In the case of water treatment, the fungus was 286.4±31.5~4,836.4±484.6 mm² in size. The results of the study confirmed that washing yuzu with ozone water has a sterilizing effect.

• Food Science of Animal Resources
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• v.35 no.3
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• pp.293-298
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• 2015

Porcine placenta extract (PPE) is known to possess anti-inflammatory properties owing to its high concentration of bioactive substances. However, the need to eliminate blood-borne infectious agents while maintaining biological efficacy raises concerns about the optimal method for sterilizing PPE. Therefore, the objective of this study was to compare the effects of the standard pressurized heat (autoclaving) method of sterilization with γ-irradiation on the anti-inflammatory effects of PPE. The anti-inflammatory actions of these two preparations of PPE were evaluated by measuring their inhibitory effects on the production of NO, the expression of iNOS protein, and the expression of iNOS, COX2, TNF-α, IL-1β, and IL-6 mRNA in lipopolysaccharide-stimulated RAW 264.7 cells. Compared with autoclaved PPE, γ-irradiated PPE showed significantly greater inhibition of NO production and iNOS protein expression, and produced a greater reduction in the expression of iNOS, COX2, TNF-α, IL-1β, and IL-6 mRNA. These results provide evidence that the sterilization process is crucial in determining the biological activity of PPE, especially its anti-inflammatory activity. Collectively, our data suggest that γ-irradiated PPE acts at the transcriptional level to effectively and potently suppresses the production of NO and the expression of pro-inflammatory cytokines.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.407-414
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• 1997

The resistance of a non-transgenic poplar clone, 'Ogy' and three transgenic poplar lines to the cottonwood leaf beetle, Chrysomela scripta F., was evaluated by in vitro feeding. The lines were transformed with neomycin phosphotransferase II(NPT II) as a selectable marker, proteinase inhibitor II(pin2) as a resistance gene, and CaMV 35S as a promoter. An efficient method of sterilizing the beetle eggs and introducing them into plant tissue cultures was developed. The resistance of the transgenic lines was investigated in terms of effects tin leaf area consumed, insect weight, insect developmental stages, and plantlet root dry weight after feeding. Also, leaf area consumed was examined by leaf age as measured through leaf plastochron index(LPI). The leaf area consumed and insect weight were highly significant between transformants and control, and insect development in vitro was significant among the transgenic lines. Larval infestation was the most severe around LPI 4 to 5 which were young leaves. The system provided a quick, highly controlled method to screen developing transgenic plantlets directly.

• Archives of Plastic Surgery
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• v.33 no.5
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• pp.627-631
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• 2006

Purpose: Ischial region is common site of pressure sore as well as greater trochanteric area. In general, ischial pressure sore associated with a large subcutaneous bursa often requires radical surgical treatment. The authors performed sclerotherapy using absolute ethanol which was considered as an alternative in treating recurrent ischial pressure sore. Methods: From may 2005 to February 2006, 11 ischial pressure sore patients were treated sclerotherapy using absolute ethanol. The authors performed sclerotherapy using absolute ethanol in 11 patients in whom the ischial sore has recurred despite of multiple radical surgical treatment. The patients' original disorders were spinal cord injury in 9 patients, cerebral palsy in 1 patient and giant cell tumor in thoracic vertebrae 1 patient. Results: Recurrence of pressure sore was not found in any patient during the follow-up period. The swap of the bursa taken before the surgery was germ cultured and compared with the discharge from an end of the inserted drain tube. The germ cultured results after the surgery were tested negative in all patients. Conclusion: This method involves causing the bursa to become scarred and closing it up by sterilizing, fixing, and denaturing by the pharmacologic effect of absolute ethanol instead of surgical excision of the bursa. We felt that aforementioned treatment modality may be considered as an alternative in treating recurrent ischial pressure sore.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.7
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• pp.269-274
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• 2013

We are used with the alkaline ion water which an application field does to object for drinking water compare with the alkaline ion water which asked ion acid electrolysis so as to be very different. This is used with sterilization disinfection use by residual chlorine in case of strong acidity according to ph intensity, and in case of middle acidity use by washing and face washing, and mix with meal materials in case of weak acidity widely usable in cooking. Acid ion water generates as we electrolyze water. Chlorine gas and sodium hydroxide etc. was generated at electrolysis process, and we have toward sterilizing power. Derelicts such as chlorine, phosphorus, sulfur etc. are gathered from a negative ion, and we make acid ion water to + electrode direction in electrolysis. We used a diaphragm in order to disconnect too acid water and alkaline water. We implemented so that the acid water which it came down to three kinds of PWM voltage to PWM (pulse width modulation) control, and implementation method of ph intensity change authorized ph intensity between weak acidity to electrode in strong acidity as we used Microprocessor, and intensity was adjusted successively by PWM control was generated.

• The Korean Journal of Health Service Management
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• v.11 no.4
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• pp.203-211
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• 2017

Objectives : This study aims to design a method for drying forceps jars. As part of the process, holes were made in the forceps jars. Drying time, rate of wet pack, and cost of rewashing linen were then determined. Moreover, the study looked into the bacteria incidence rate in the forceps jars. This study suggests a practical way for preventing infection in a hospital through a surgical device. Methods : This study investigated the effect of forceps jars with holes on reducing drying time, rate of wet pack, and cost of rewashing linen. It also looked into the bacteria incidence rate in the forceps jars through a parallel design. Data was collected and analyzed using SPSS version 21. Results : The drying time of a forceps jars was remarkably reduced from $18.44{\pm}0.2$ minutes(non-hole forceps jars) to $0.58{\pm}0.02$ minutes(4-hole forceps jars). The rate of wet pack of non-hole forceps jars was 51.4%. However, the rate of 4-hole forceps jars reached 4.6%. The cost of rewashing linen saw a decline from 38,073 won to 3,381 won. In addition, no bacteria and virus were detected from the 24-hours usage of forceps jars even though the jars had holes. Conclusions : The 4-hole forceps jars greatly increased work efficiency as a result of the reduced drying time, rate of wet-pack and cost of rewashing linen. The 4-hole forceps jars did not influence their sterilizing effect.

• Food Science and Preservation
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• v.11 no.2
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• pp.246-249
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• 2004

This study was conducted to evaluate the inhibition efficacy of high CO$_2$ atmosphere (35, 60, and 100% ${\times}$ 24, 48, and 72 h) on growth of Botrytis cinerea in vitro in order to offer sterilizing method of horticultural crops including peach fruits. Botrytis cinerea was isolated from a naturally infected peach fruits. Growth of the fungus at 25$^{\circ}C$ declined with increased CO$_2$ concentration and treatment duration. Especially, 100% CO$_2$ provided completely inhibition effect of growth of the fungus for 72 h. After removal of high CO$_2$ condition, however, the fungus showed normal growth speed. The growth of fungus at low temperature was completely inhibited temporarily by short-term 100% CO$_2$ treatment, but resumed right after transferring to normal atmosphere at 25$^{\circ}C$.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.18 no.8
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• pp.67-73
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• 2019

An ultra-high pressure treatment device is a device used for increasing the shelf life of food by sterilizing it by applying hydrostatic pressure to solid or liquid food. The ultrahigh pressure treatment system developed in this study is a pressure vessel with a processing capacity of 100 L and a maximum pressure of 700 MPa. Pressure vessels for ultrahigh-pressure processing equipment are manufactured using wire-winding techniques. The design formula for making ultra-high pressure vessels with wire windings is given in ASME Section VIII - Division 3. In this study, the ratio of the cylinder to the winding area that can be applied in a wire-winding application was analyzed using a finite element analysis. Furthermore, the relationship between the variation of the residual stress in the vessel and the ratio of the winding area due to the variation of the winding tension was analyzed, and a design guide applicable to the actual product design was developed. Finally, the design equation was modified by presenting the coefficients to correct the difference between the finite element analysis and the design equation.

• Biomedical Science Letters
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• v.27 no.4
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• pp.264-269
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• 2021

The purpose of this study was to evaluate the disinfecting efficacy of peracetic acid (PAA), sodium hypochlorite (NaOCl) and phenol, which are representative disinfectants in medical environments using four types of multi-drug resistance (MDR) clinical isolates with healthcare-associated infections (HAI). 26 antibiotic susceptibility tests were conducted for the four types of MDR clinical isolates in the same way as for clinical specimens. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the disinfectants were determined by using in vitro liquid medium dilution method and inoculation of the plate medium. Both the MIC and MBC of phenol against MRSA and VRE were 3.1%, while those against KPC and MRPA were 6.2%. The MIC and MBC of peracetic acid (PAA) against MRSA, VRE, KPC, and MRPA were 0.18%. The MIC and MBC of sodium hypochlorite (NaOCl) against MRSA were 0.39% and 0.78%, respectively. Both values of MIC and MBC were 0.78% for VRE. In addition, KPC and MRPA showed 0.39% for MIC and 0.78% for MBC. For all MDR strains used in this study, sodium hypochlorite and peracetic acid showed significant sterilizing efficiency, while no clear correlation was identified between antibiotic resistance clinical isolated and ability of disinfection.

• Journal of Korean Clinical Nursing Research
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• v.15 no.1
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• pp.55-65
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• 2009

Purpose: The purpose of this study was to compare the 1% chlorhexidine gluconate/61% ethanol (CHG/ethanol), 45% ethanol/18% 1-propanol (ethanol/propanol) and 7.5% povidone-iodine (PVI) scrub with brush to evaluate their antimicrobial effect. Method: Utilizing repeated measures design, 9 nurses participated in the study. Glove juice sampling procedure was used to evaluate microbial hand counts before the surgical hand antisepsis, one minute after hand wash, and after the surgery. Results: Waterless rub using CHG and ethanol combination resulted in a 3.94 log reduction at 1 min and 2.78 log reduction at 3 hrs. Ethanol/propanol resulted in a 2.42 at 1 min and 2.22 at 3 hrs. The traditional scrub using PVI with brush resulted in a 0.94 at 1 min and 0.95 at 3 hrs (p=.003) and 3 hrs (p=.026) after the surgical hand antisepsis. Repeated measures ANOVA results showed that there was a statistically significant difference among group (p=.002). Duncan post hoc test result showed that the PVI was less effective (p<.05) in sterilizing microbials on hands than CHG/ethanol or ethanol/propanol. Conclusion: Both of the two alcohol-based antiseptic rubs are acceptable alternatives to the PVI with brush for surgical hand antisepsis.