• 한국발생생물학회지:발생과생식
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• 제14권3호
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• pp.155-162
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• 2010

The trophectoderm is one of the earliest cell types to differentiate in the forming placenta. It is an important for the initial implantation and placentation during pregnancy. Trophoblast stem cells (TBSCs) develop from the blastocyst and are maintained by signals emanating from the inner cell mass. However, several limitations including rarity and difficulty in isolation of trophoblast stem cells derived from blastocyst still exist. To establish a model for trophoblast differentiation, we isolated TBSCs from human term placenta ($\geq$38 weeks) and characterized. Cell cycle was analyzed by measuring DNA content by FACS analysis and phenotype of TBSCs was characterized by RT-PCR and FACS analysis. TBSCs have expressed various markers such as self-renewal markers (Nanog, Sox2), three germ layer markers (hNF68, alpha-cardiac actin, hAFP), trophoblast specific markers (CDX-2, CK7, HLA-G), and TERT gene. In FACS analysis, TBSCs isolated from term placenta showed that the majority of cells expressed CD13, CD44, CD90, CD95, CD105, HLA-ABC, cytokeratin 7, and HLA-G. Testing for CD31, CD34, CD45, CD71, vimentin and HLA-DR were negative. TBSCs were shown to decrease the growth rate when cultured in conditioned medium without FGF4/heparin as well as the morphology was changed to a characteristic giant cell with a large cytoplasm and nucleus. In invasion assay, TBSCs isolated from term placenta showed invasion activities in in vivo using nude mice and in vitro Matrigel system. Taken together, these results support that an isolation potential of TBSCs from term placenta as well as a good source for understanding of the infertility mechanism.

• 컴퓨터교육학회논문지
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• 제22권3호
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• pp.37-54
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• 2019

STEM 융합교육의 중요한 목적은 서로 다른 학문이 가지는 탐구의 방법을 이해함으로써 융합적 문제해결력을 기르는 것이다. 이를 위해 우선적으로 각 학문에서 중요하게 다루어지는 문제해결과정을 이해해야 한다. 본 연구는 과학(S), 공학(E), 수학(M) 각각의 분야에서 어떻게 문제해결과정을 정의하고 있는지 비교분석하고, 이를 근거로 SEM 문제해결과 CT 문제해결의 관련성을 파악하고자 하였다. 이를 위해 먼저 SEM 각 학문의 문제해결과정을 비교 분석하여 그 공통점과 차이점을 기술하였다. 다음으로 CT를 도구적 측면과 사고적 측면으로 구분하고 문제해결과정으로서 CT가 SEM 각각의 학문에서 문제해결과 어떤 차이가 있는지 기술하였다. 마지막으로, SEM 문제해결 프로세스와 CT와의 관계를 모형으로 제시하였다. 본 연구는 문제해결과정으로써 CT와 SEM이 융합할 수 있는 방향을 제시한다는 점에서 그 의미가 있다.

• 한국동물생명공학회지
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• 제38권4호
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• pp.199-208
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• 2023

Background: Canine induced pluripotent stem cells (iPSCs) are an attractive source for veterinary regenerative medicine, disease modeling, and drug development. Here we used vitamin C (Vc) to improve the reprogramming efficiency of canine iPSCs, and its functions in the reprogramming process were elucidated. Methods: Retroviral transduction of Oct4, Sox2, Klf4, c-Myc (OSKM), and GFP was employed to induce reprogramming in canine fetal fibroblasts. Following transduction, the culture medium was subsequently replaced with ESC medium containing Vc to determine the effect on reprogramming activity. Results: The number of AP-positive iPSC colonies dramatically increased in culture conditions supplemented with Vc. Vc enhanced the efficacy of retrovirus transduction, which appears to be correlated with enhanced cell proliferation capacity. To confirm the characteristics of the Vc-treated iPSCs, the cells were cultured to passage 5, and pluripotency markers including Oct4, Sox2, Nanog, and Tra-1-60 were observed by immunocytochemistry. The expression of endogenous pluripotent genes (Oct4, Nanog, Rex1, and telomerase) were also verified by PCR. The complete silencing of exogenously transduced human OSKM factors was observed exclusively in canine iPSCs treated with Vc. Canine iPSCs treated with Vc are capable of forming embryoid bodies in vitro and have spontaneously differentiated into three germ layers. Conclusions: Our findings emphasize a straightforward method for enhancing the efficiency of canine iPSC generation and provide insight into the Vc effect on the reprogramming process.

• BMB Reports
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• 제41권1호
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• pp.23-28
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• 2008

The altered amino acid (AA) levels as neurotransmitter closely correlate to neurodegenerative conditions including Alzheimer's disease (AD). Target profiling analysis of nineteen AAs in brain cortex samples from three Tg2576 mice as AD model and three littermate mice as control model was achieved as their N(O,S)-ethoxycarbonyl/tert-butyldimethylsilyl derivatives by gas chromatography. Subsequently, star pattern recognition analysis was performed on the brain AA levels of AD mice after normalization to the corresponding control median values. As compared to control mice, $\gamma$-aminobutyric acid among ten AAs found in brain samples was significantly reduced (P < 0.01) while leucine was significantly elevated (P < 0.02) in AD mice. The normalized AA levels of the three AD mice were transformed into distorted star patterns which was different from the decagonal shape of control median. The present method allowed visual discrimination of the three AD mice from the controls based on the ten normalized AA levels.

• Archives of Pharmacal Research
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• 제26권6호
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• pp.458-462
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• 2003

The antioxidant activity of the stem bark from Albizzia julibrissin was evaluated for its potential to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, to inhibit the generation of the hydroxyl radical ($\cdot OH$), total reactive oxygen species (ROS) and to scavenge authentic peroxynitrites ($ONOO^{-}$). The methanol extract of A. julibrissin exhibited strong antioxidant activity in the tested model systems. Therefore, it was further fractionated using several solvents. The antioxidant activity of the individual fractions were in the order of ethyl acetate (EtOAc) ＞ n-butanol (n-BuOH) ＞ dichloromethane ($CH_2 CI-2$) ＞ and water ($H_2O$). The ethyl acetate soluble fraction, which exhibited strong antioxidant activity, was further purified by repeated silicagel, Sephadex LH-20 and RP-18 gel column chromatography. Sulfuretin (1) and 3 ,4 ,7-trihydroxyflavone (2) were isolated as the active principles. Compounds 1 and 2 exhibited good activity in all tested model systems. Compound 1 exhibited five times more inhibitory activity on the total ROS than Trolox. Compound 2 showed six times stronger DPPH radical scavenging activity than L-ascorbic acid. These results show the possible antioxidant activity of the A. julibrissin crude extract and its major constituents.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.58-58
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• 2022

With the development of advanced technology, automation of agricultural work is spreading. In association with the 4th industrial revolution-based technology, research on field smart farm technology is being actively conducted. A state-of-the-art unmanned automated agricultural production demonstration complex was established in Naju-si, Jeollanam-do. For the operation of the demonstration area platform, it is necessary to build a sophisticated, advanced, and intelligent field smart farming model. For the operation of the unmanned automated agricultural production demonstration area platform, we are building data on the growth of soybean for smart cultivated crops and conducting research to determine the optimal time for agricultural work. In order to operate an unmanned automation platform, data is collected to discover digital factors for water management immediately after planting, water management during the growing season, and determination of harvest time. A subsurface drip irrigation system was established for smart water management. Irrigation was carried out when the soil moisture was less than 20%. For effective water management, soil moisture was measured at the surface, 15cm, and 30cm depth. Vegetation indices were collected using drones to find key factors in soybean production prediction. In addition, major growth characteristics such as stem length, number of branches, number of nodes on the main stem, leaf area index, and dry weight were investigated. By discovering digital factors for effective decision-making through data construction, it is expected to greatly enhance the efficiency of the operation of the unmanned automated agricultural production demonstration area.

• Journal of Korean Neurosurgical Society
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• 제56권5호
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• pp.383-389
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• 2014

Objective : Neural tissue transplantation has been a promising strategy for the treatment of Parkinson's disease (PD). However, transplantation has the disadvantages of low-cell survival and/or development of dyskinesia. Transplantation of cell aggregates has the potential to overcome these problems, because the cells can extend their axons into the host brain and establish synaptic connections with host neurons. In this present study, aggregates of human brain-derived neural stem cells (HB-NSC) were transplanted into a PD animal model and compared to previous report on transplantation of single-cell suspensions. Methods : Rats received an injection of 6-OHDA into the right medial forebrain bundle to generate the PD model and followed by injections of PBS only, or HB-NSC aggregates in PBS into the ipsilateral striatum. Behavioral tests, multitracer (2-deoxy-2-[$^{18}F$]-fluoro-D-glucose ([$^{18}F$]-FDG) and [$^{18}F$]-N-(3-fluoropropyl)-2-carbomethoxy-3-(4-iodophenyl)nortropane ([$^{18}F$]-FP-CIT) microPET scans, as well as immunohistochemical (IHC) and immunofluorescent (IF) staining were conducted to evaluate the results. Results : The stepping test showed significant improvement of contralateral forelimb control in the HB-NSC group from 6-10 weeks compared to the control group (p<0.05). [$^{18}F$]-FP-CIT microPET at 10 weeks posttransplantation demonstrated a significant increase in uptake in the HB-NSC group compared to pretransplantation (p<0.05). In IHC and IF staining, tyrosine hydroxylase and human ${\beta}2$ microglobulin (a human cell marker) positive cells were visualized at the transplant site. Conclusion : These results suggest that the HB-NSC aggregates can survive in the striatum and exert therapeutic effects in a PD model by secreting dopamine.

• 한국산림과학회지
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• 제107권2호
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• pp.184-192
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• 2018

본 연구는 중부지역 일본잎갈나무의 수간중량 추정식을 개발하기 위하여 지역별 개체목의 지위와 직경을 고려하여 표준지를 선정한 뒤, 총 55본의 표본목을 선정하였다. 표본목의 실측자료를 이용하여 수피외생중량, 수피외건중량, 수피내 건중량 및 이용중량 등 네종류의 중량을 추정하기 위해 11개의 모형을 비교 분석하였다. 중량을 추정하기 위해 흉고직경을 변수로 이용하는 경우, 흉고직경과 수고를 이용하는 경우, 재적을 이용하는 경우의 3개 변수에 따라 모형을 구분하였다. 최적의 모형은 적합지수와 추정의 표준오차 및 잔차의 분포를 이용하여 모형의 이행능력을 비교하여 선정하였다. 그 결과, 1변수인 흉고직경을 이용하는 식은 $W=a+bD+c^D^2$이며 설명력은 90~92%를 나타냈고, 흉고직경과 수고를 이용한 2변수식은 $W=aD^bH^C$으로 97~98%의 설명력으로 2변수 모형이 1변수 모형 보다 높은 적합도를 보였다. 또한 전체수간에 대한 재적과 이용재적에 대한 식인 W = aV로 중량추정설명력이 98~99%으로 높게 나타났고 SEE도 7.7~17.5, CV(%)도 8.0~10.0으로 우세한 적합도를 보였다. 본 연구는 개체목의 중량정보 제공 및 임분단위의 중량 바이오매스추정식의 기초연구로 활용될 것으로 판단된다.

• Reproductive and Developmental Biology
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• 제36권4호
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• pp.275-281
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• 2012

Embryonic stem cell-preconditioned microenvironment is important for cancer cells properitities by change cell morphology and proliferation. This microenvironment induces cancer cell reprogramming and results in a change in cancer cell properties such as differentiation and migration. The cancer microenvironment affects cancer cell proliferation and growth. However, the mechanism has not been clarified yet. Using the ES-preconditioned 3-D microenvironment model, we provide evidence showing that the ES microenvironment inhibits proliferation and reduces oncogenic gene expression. But ES microenvironment has no effect on telomerase activity, cell viability, cellular senescence, and methylation on Oct4 promoter region. Furthermore, methylation of Nanog was increase on ES-preconditioned microenvironment and supports results that no difference on RNA expression levels. Taken together, these results demonstrated that in the ES-preconditioned 3-D microenvironment is a crucial role for cancer cell proliferation not senescence.

• BMB Reports
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• 제48권4호
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• pp.223-228
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• 2015

The Drosophila lymph gland is the hematopoietic organ in which stem-like progenitors proliferate and give rise to myeloid-type blood cells. Mechanisms involved in Drosophila hematopoiesis are well established and known to be conserved in the vertebrate system. Recent studies in Drosophila lymph gland have provided novel insights into how external and internal stresses integrate into blood progenitor maintenance mechanisms and the control of blood cell fate decision. In this review, I will introduce a developmental overview of the Drosophila hematopoietic system, and recent understandings of how the system uses developmental signals not only for hematopoiesis but also as sensors for stress and environmental changes to elicit necessary blood responses. [BMB Reports 2015; 48(4): 223-228]