• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.4
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• pp.572-577
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• 2002

We investigated the optimal condition for production of bacterial cellulose with Gluconacetobacter persimmonus KJ145. For bacterial cellulose production, optimal medium composition and culture conditions were conducted to determine. Apple juice (10$^{\circ}$Brix) medium was suitable than Hestrin & Schramm medium which is generally used for the bacterial cellulose production. When 1% pyruvate as carbon source was added to apple juice, bacterial cellulose production rose to high level. The effect of various nitrogen sources was investigated: CSL was found to be essential to high cellulose yields and the optimal CSL concentration was 10%. Optimal temperature and culture time for the bacterial cellulose production was 35$^{\circ}C$ and 16 days, respectively At the optimal condition Gluconacetobacter persimmonus KJ145 produced 8.96g/L of bacterial cellulose (dry weight), which was much higher than reported values.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.469-472
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• 2002

Solid state fermentation was performed for the production of entomopathogenic fungus Metarhizium anisopliae HY-2 using wheat bran media containing rice bran. Fungal growth in a solid state fermentation system was estimated by viable cell count, spore count, and mycelial biomass. It was used chemical method measuring N-acetyl-glucosamine (chitin) content for estimating of mycelial biomass. In static flask culture, viable cell reached 2.40 ${\times}$ $10^8$ cfu/g at 23 days of culture at $27^{\circ}C$ and then mycelial biomass was 41.59 mg/g. Specific growth rate(${\mu}$ max) was 0.0418 $h^{-1}$ between 3 and 9 days when estimated by viable cell count and was 0.00976 $h^{-1}$ between 9 and 17 days when N-acetylglucosamine content was measured. Viable cells reached 1.12 ${\times}$ $10^8$ cfu/g in polypropylene-bag at 28 days of culture at $27^{\circ}C$. Formulated microbial pesticide containing M. anisopliae HY-2 were tested their bio-activity against Chestnut Brown Chafer (Adoretus tenuimaculatus). The protection rate of the liquid culture showed 13 ${\sim}$ 26 % with 1st to 3rd instar, and spore suspension of M. anisopliae HY-2 showed 56 ${\sim}$ 64%. Conidia produced by large scale solid-state fermentation showed 20 ${\sim}$ 27 % activity 60 ${\sim}$ 64 % with M. anisopliae HY-2.

• Korean Journal of Heritage: History & Science
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• v.48 no.2
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• pp.48-63
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• 2015

This study began with the purpose of awakening an interest in Korean Nu-jung and seeking application plan based on Nu-jung of Aneuisamdong. Also it intends to suggest activation plan of Nu-jung while using the culture of taste for the arts and classical scholar of ancestors with the physical place named Nu-jung as the medium. As concrete ways to activate Nu-jung, we suggested plans which use the game of go(Baduk), main material of the culture of taste for the arts and classical scholar, and increase availability by planning a program adding picturesqueness of Nu-jung. By bringing the material named the game of go(Baduk) to the place named Nu-jung, we suggested the plan capable of experiencing the culture of taste for the arts and classical scholar and also expecting local promotion and economical effect. And the plan is to design and conduct night program, 1 night 2 days program, tailored program, and so on by combining the material of the culture of taste for the arts and classical scholar with the medium named 'Dal(the moon)' in the static place named Nu-jung. The plans above are applicable to most of Nu-jung scattered all over the country, which means that we have a lot of Nu-jung in Korea and, at the same time, we have abundant contents to be used as cultural properties. Now it's important how to develop and use these contents through the connection with various academic fields in terms of managing cultural properties. By understanding the value of the original form of culture while emerging from simply using cultural properties, we can use cultural properties in a higher level. For this, preserving cultural properties should be supported by continuous interdisciplinary study.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.344-348
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• 2000

Abstract The removal percentage (94%) of 100 ppm of pyrene in a shaken culture of white rot fungus, Irpex lacteus, was much higher than that in a static culture (37.9%). Over 90% of the pyrene disappeared with I. lacteus grown at $15-27^{\circ}C$, yet less than 50% was removed at $37^{\circ}C$. The transformation rates of pyrene ($4.5-5.0{\;}\mu\textrm{g}/ml/day$) were not very different among cultures with 5- 30% inoculum sizes, and over 90% of the 100 ppm pyrene was removed in every case during 20 days of incubation. The biodegradation of pyrene by I. lacteus was confirmed by measuring the $CO_2$ evolved from the mineralization of the added pyrene. The activity of lignin peroxidase (LiP), which is known to be involved in the biodegradation by white rot fungi, was high between 8 to 12 days of incubation. Although manganese peroxidase activity was demonstrated during the same period as LiP, its activity was quite low, and no laccase activity was detected. Even though the activity patterns of ligninolytic enzymes did not coincide with the pyrene removal, this study shows that I. lacteus has a high biodegrading capability and can be a candidate for the bioremediation of polycyclic aromatic hydrocarbon contaminants.inants.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.723-726
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• 2001

This study was performed to improve the yield of bacterial cellulose(BC) by UV and NTG mutagenesis of strain KJ-1 which produced largely BC. some mutants showed high BC productivity with twice elevation compared to that the wild strain KJ-1. A difference was found in production and bioconversion phase of synthesized organic acid, such as gluconic acid, 2-keto gluconic acid, and 5-keto gluconic acid between mutants and strain KJ-1 in the static culture. The organic acid produced in secondary metabolism phase, were more rapidly consumed in the culture with the mutants than that the parent strain after glucose in the broth was conversed to a limiting substrate. Therefore, we suggested the reason for increasing of BC production that the mutant strain consumed more efficiently synthesized acids as substrates than that of the parent strain.

• The Journal of the Convergence on Culture Technology
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• v.2 no.4
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• pp.49-54
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• 2016

Development of AR/VR has expanded computer games from indoor static contents to outdoor dynamic contents. The syndrome of 'Pokemon Go' which is a representative AR game was born, and Nintendo's stock price shot up a massive 120%. Although some people gained profits, new damages are emerging including illegal intrusion or road accidents because it is a type of game to visit real locations. There are reported about 114 thousand road accidents related to the game. This study aims to examine the trends of AR game in 2016 and analyze damages and positive cases to suggest a method for preventing accidents due to the AR game, and a business model.

• The Journal of the Convergence on Culture Technology
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• v.3 no.2
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• pp.31-35
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• 2017

Recently, Many deliveries have sprung up and those are beginning to receive much attention from consumers. Like this, the application makes our lives more comfortable. Now, the delivery food application uses only static information of the user and the users only makes oder. In this thesis, however, the studies suggested that designed food application uses the user's location for mesuring a distance of delivery and estimated arrival time is displayed in the delivery application and The application provides a graphical view of the location of the user in real time.

• International Journal of Advanced Culture Technology
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• v.6 no.3
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• pp.163-172
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• 2018

The power train of hydro-mechanical continuously variable transmission(HMCVT) for the middle class forklift makes use of an hydro-static unit, hydraulic multi-wet disc brake & clutches and complex helical & planetary gears. The complex helical & planetary gears are a very important part of the transmission because of strength problems. The helical & planetary gears belong to the very important part of the HMCVT's power train where strength problems are the main concerns including the gear bending stress, the gear compressive stress and scoring failures. The present study, calculates specifications of the complex helical & planetary gear train and analyzes the gear bending and compressive stresses of the gears. It is necessary to analyze gear bending and compressive stresses confidently for an optimal design of the complex helical & planetary gears in respect of cost and reliability. This paper not only analyzes actual gear bending and compressive stresses of complex helical & planetary gears using Lewes & Hertz equation, but also verifies the calculated specifications of the complex helical & planetary gears by evaluating the results with the data of allowable bending and compressive stress from the Stress - No. of cycles curves of gears. In addition, this paper explains actual gear scoring and evaluates the possibility of scoring failure of complex helical & planetary gear train of hydro-mechanical continuously variable transmission for the forklift.

• The Research Journal of the Costume Culture
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• v.19 no.5
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• pp.1019-1030
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• 2011

The aim of this study was to develop a pattern of flight attendant uniform shirts to provide better comfort for their work postures. Flight attendants' work postures were evaluated to determine the problems of clothing and mobility during their work. The pattern of the flight attendants' uniform shirt was altered by applying dynamic wearing ease(DWE). DWE was calculated from four standardized dynamic postures and a static posture. An experimental garment was made with the altered postures. The researcher redesigned the pattern of the uniform shirts, which minimizes physical limitations in movements. The fit and mobility of the shirts were evaluated. Results of this study are as follows. First, the five representative work postures were selected by "clothing stress" and "repetitiveness." These postures included raised arms, twisting midriff and shoulder postures. Five representative postures were selected by using the ergonomic posture assessment device index(OWAS). Second, the experimental garment was developed by applying DWE across the back and at armhole depth, back length, and side length. Third, the fit and mobility of the experimental garments and the original uniform shirts were compared at the flight working environment set and 5 dynamic body postures of raising arms. The experimental garments made with an altered pattern provided better fit and mobility than the original sample shirts.

• The Research Journal of the Costume Culture
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• v.19 no.5
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• pp.1049-1060
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• 2011

The purpose of this study was to develop a shirt pattern that enhances the functionality of golf swing motions. The pattern was made with 3D body surface shape data that changed according to dynamic golf postures. The data were collected from the golf swing motions. The 3D body surface data in golf swing postures piled up on the 3D surface data in a static posture. The results showed that the surface shape data changed more in the address, back swing, and finish postures than the other swing postures. The experimental pattern was developed with 3D surface scan data in those three golf swing motions. The pattern had raglan sleeves and the front-bodied piece was divided into two pieces with a princess line, which comes from armscye line of the address posture. The back bodice piece was divided into three pieces with a yoke line and a back princess line. The yoke line was made by back shoulder shape in the back swing posture. The level of comfort of the experimental garment and commercial golf shirts was evaluated by 38 women golfers. The experimental garment pattern was evaluated to be more comfortable in golf swing postures than commercial golf shirts.