• Title/Summary/Keyword: stamp

Search Result 448, Processing Time 0.03 seconds

Protein Array Fabricated by Microcontact Printing for Miniaturized Immunoassay

  • Lee Woo-Chang;Lim Sang-Soo;Choi Bum-Kyoo;Choi Jeong-Woo
    • Journal of Microbiology and Biotechnology
    • /
    • v.16 no.8
    • /
    • pp.1216-1221
    • /
    • 2006
  • A protein array was fabricated for a miniaturized immunoassay using microcontact printing ($\mu$CP). A polydimethylsiloxane (PDMS) stamp with a 5 $\mu$m$\times$5 /$\mu$m dimension was molded from a silicon master developed by photolithography. Under optimal fabrication conditions, including the baking, incubation, and exposure time, a silicon master was successfully fabricated with a definite aspect ratio. An antibody fragment was utilized as the ink for the $\mu$CP, and transferred to an Au substrate because of the Au-thiol (-SH) interaction. The immobilization and antibody-antigen interaction were investigated with fluorescence microscopy. When human serum albumin (HSA) was applied to the protein array fabricated with an antibody against HSA, the detection limit was 100 pg/ml of HSA when using a secondary antibody labeled with a fluorescence tag. The fabricated protein array maintained its activity for 14 days.

Improved Defect Control Problem using Scaled Down Silicon Oxide Stamps for Nanoimprint Lithography (나노임프린트 리소그래피를 위한 스케일 다운된 산화막 스탬프 제작과 패턴결함 개선에 관한 연구)

  • Park, Hyung-Seok;Choi, Woo-Beom;Sung, Man-Young
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.19 no.2
    • /
    • pp.130-138
    • /
    • 2006
  • We have investigated pattern scaling down of silicon stamps through the oxidation technique, During oxidizing the silicon stamps, silicon dioxide that has 300 nm and 500 nm thickness was grown, and critical deformations were not observed in the patterns. There was positive effect to reduce size of patterns because vertical and horizontal patterns have different orientation. We achieved pattern reduction rate of $26\%$. In addition, the formation of polymer patterns had been investigated with varied temperature and pressure conditions to improve the filling characteristics of polymers during nanoimprint lithography when pattern sizes were few micrometers. In these varied conditions, polymers had been affected by free space compensation and elastic stress relaxation for filling the cavities. Based on the results, defect control which is an important issue in the nanoimprint lithography were facilitated.

Analysis of Wrinkling and Die Design for Welded Blank Hydroforming of Automotive Subframe (자동차 서브프레임 용접판재 유압성형시 주름발생 원인분석과 금형설계)

  • Kim H. Y.;Shin Y. S.;Hong C. K.;Jeon B. H.;Oh S. I.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
    • /
    • 2000.10a
    • /
    • pp.153-157
    • /
    • 2000
  • This paper presents possible defects when welded blank hydroforming and focus on wrinkling, and the die design to prevent this defect An explicit finite element code, PAM-STAMP, is used to simulate welded blank hydroforming process. The numerical results are compared to the experimental results in the aspect of deformed shape. An automobile subframe is taken as an example to carry out finite element analysis.

  • PDF

A Clock Regenerator using Two 2nd Order Sigma-Delta Modulators for Wide Range of Dividing Ratio

  • Oh, Seung-Wuk;Kim, Sang-Ho;Im, Sang-Soon;Ahn, Yong-Sung;Kang, Jin-Ku
    • JSTS:Journal of Semiconductor Technology and Science
    • /
    • v.12 no.1
    • /
    • pp.10-17
    • /
    • 2012
  • This paper presents a clock regenerator using two $2^{nd}$ order ${\sum}-{\Delta}$ (sigma-delta) modulators for wide range of dividing ratio as defined in HDMI standard. The proposed circuit adopts a fractional-N frequency synthesis architecture for PLL-based clock regeneration. By converting the integer and decimal part of the N and CTS values in HDMI format and processing separately at two different ${\sum}-{\Delta}$ modulators, the proposed circuit covers a very wide range of the dividing ratio as HDMI standard. The circuit is fabricated using 0.18 ${\mu}m$ CMOS and shows 13 mW power consumption with an on-chip loop filter implementation.

Preparation of Antistiction Coatings for Nanoimprinting (나노임프린팅 공정을 위한 점착방지막 형설)

  • Cha, N.G.;Park, C.H.;Kim, K.C.;Park, J.G.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
    • /
    • 2006.05a
    • /
    • pp.86-90
    • /
    • 2006
  • Nanoimprint lithography (NIL) is a novel method to fabricate nanometer scale patterns. It is a simple process with low cost, high throughput and high resolution. NIL process creates patterns by the mechanical deformation of imprint resist and physical contact process. This physical contact process causes the stiction between the resist and the stamp. Stiction becomes a key issue especially in the stamps including narrow pattern size and wide area during NIL process development. The antistiction layer coating using fluorocarbon is very effective to prevent this problem and ensure successful NIL. In this paper, the concept of antistiction coating is explained and different preparation methods for nanoimprinting are briefly discussed.

  • PDF

What is Natural History\ulcorner (지역사란 무엇인가\ulcorner)

  • Choe, Jae Chun
    • The Korean Journal of Ecology
    • /
    • v.18 no.4
    • /
    • pp.525-531
    • /
    • 1995
  • The Korean government has recently announced its plan to establish the first National Museum of Natural History. However, the Korean word for natural history, jayonsa, is not a very familiar term to some academics as well as the general public. This article discusses the definition and history of natural history, describes the functions of natural history meseums, and makes suggestions to the establishment of our National Museum. Modern natural history is no longer an art of ‘stamp collecting’. It is a comprehensive scientific endeavor pursuing to enlighten the history of the planet Earth and the diversity of natural objects it contains. Natural history museum must have two museums within the museum: the outer museum for exhibition and general public education and the inner museum for research and specialist education. I hope that our National Museum of Natural History will be a place where we all get to know about Nature and thus to love her.

  • PDF

Development of Fabrication Process of Light Guiding Plate with Nanometer-Sized-Cylindrical Pattern Using Nano Imprint Lithography Method (나노 임프린트 리소그래피법에 의한 나노미터급 원기둥 패턴을 갖는 도광판의 제작 공정 개발)

  • Lee, Byoung-Wook;Hong, Chin-Soo;Kim, Chang-Kyo
    • Journal of Institute of Control, Robotics and Systems
    • /
    • v.14 no.4
    • /
    • pp.332-335
    • /
    • 2008
  • PMMA light guiding plate with nano pattern was fabricated by nano imprint lithography method. A silicon mold for electroplating of nickel was fabricated by conventional photolithography process. A nickel stamp for nano imprint lithography was fabricated by electroplating process using silicon mold. The nano imprint lithography was performed on PMMA plate at $140^{\circ}C$ under pressure of 20kN. The nano pattern on PMMA plate was investigated using FE-SEM. It is shown that the patterns were well transferred for several steps and the nano imprint lithography method could be applied for fabricating patterns of light guiding plate.

Nano Molding Technology for Optical Storage Media with Large-area Nano-pattern (대면적 광 정보저장매체의 나노성형에 대한 기술 개발)

  • Shin Hong-Gue;Ban Jun-Ho;Cho Ki-Chul;Kim Heon-Yong;Kim Byeong-Hee
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.23 no.4 s.181
    • /
    • pp.162-167
    • /
    • 2006
  • Hot embossing lithography(HEL) has the production advantage of comparatively few process step, simple operation, a relatively low cost for embossing tools(Si), and high replication accuracy for small features. In this paper, we considered the nano-molding characteristic according to molding parameters(temperature, pressure, times, etc) and induced a optimal molding condition using HEL. High precision nano-patter master with various shapes were designed and manufactured using the DRIE(Deep Reactive ion Etching), LPCVD(Low Pressure Chemical Vapor Deposition) and thermal oxidation process, and we investigated the molding characteristic of DVD and Blu-ray nickel stamp. We induced flow behaviors of polymer, rheology by shapes and sizes of the pattern through various molding experiments. Finally, with achieving nano-structure molding with high aspect ratio, we will secure a basic technology about the molding of large-area nano-pattern media.

Finite Element Analysis for Forming Processes of $\Omega$-type Bellows Tube (오메가형 벨로즈관의 성형을 위한 유한요소해석)

  • 이정훈;김낙수;전병희
    • Proceedings of the Korean Society for Technology of Plasticity Conference
    • /
    • 1997.03a
    • /
    • pp.165-170
    • /
    • 1997
  • The study presents an computer-aided analysis and its design for the forming process of $\Omega$-type bellows tube. Finite element analysis was carried out to perform the process simulation. Based on the analytic results of various conditions, the forming conditions used for angled U-type bellows tube were settled. The 3D modeling was constructed by I-DEAS and PAM-STAMP was used for process simulation. It is concluded that the spring back of formed bellows influences $\Omega$-shape and these results can be used for the process design.

  • PDF

Giardiasis in Black-tailed Prairie Dogs (Cynomys ludovicianus) (검은 꼬리 프레리 독(Cynomys ludovicianus)의 편모충증)

  • Cho Ho-Seong;Kim Young-Seob;Jeong Young-Mok;Seung Won-Woo;Park Nam-Yong
    • Journal of Veterinary Clinics
    • /
    • v.22 no.4
    • /
    • pp.417-419
    • /
    • 2005
  • Five black-tailed prairie dogs showed weakness. emaciation and anemia during the lairage period in May 2005. Among them, one adult male and one female prairie dogs were found dead. The stamp smear for revealed a tremendous number of Giardia trophozoites. Histopathological examinations of the intestine showed slightly inflammatory changes and cystic enlargement of the crypts in the duodenal and jejunal mucous membranes. From these findings, this disease was diagnosed as giardiasis in black-tailed prairie dog.