• 한국식생활문화학회지
• /
• 제8권2호
• /
• pp.147-151
• /
• 1993

The composition of isothiocyanates in Dolsan leaf mustard was investigated. Five major volatile isothiocyanates detected in leaf mustard were sec-butyl isothiocyanate, allyl isothiocyanate, 3-butenyl isothiocyanate, n-hexyl isothiocyanate and ${\beta}-phenylethyl$ isothiocyanate. In both leaf and leaf stalk allyl isothiocyanate and 3-butenyl isothiocyanate were the most abundant. The compositional difference of isothiocyanates between leaf and leaf stalk was that n-hexyl and ${\beta}-phenylethyl$ isothiocyanates were present more in leaf than leaf stalk.

• 생물환경조절학회지
• /
• 제18권4호
• /
• pp.448-453
• /
• 2009

본 연구에서는 참외 추출물의 항암활성에 대해 알아보기 위해 참외를 부위별로 나누어 quinone reductase 유도활성과 다양한 간암세포에서의 증식 억제활성을 조사하였다. 참외 꼭지와 참외 줄기 잎 부위에서 농도의존적으로 QR 유도활성이 증가하였고, $200{\mu}g/mL$ 농도에서는 각각 3.9, 1.5배의 유도활성을 나타내었다. 암세포 사멸 활성 측정법을 통한 항암활성 평가 실험에서 마우스 유래의 간암세포인 Hepa1c1c7 세포에 대해서 조사한 결과 꼭지와 줄기 잎 부위에서 높은 암세포 독성을 보였다. 이러한 결과를 기초로 인체유래의 암세포에 대한 항암활성을 평가하기 위해 인체유래 간암 세포주인 HepG2에 대한 세포 증식 억제활성을 농도별로 조사하였다. 꼭지와 줄기 잎 부위 모두 인체유래 간암 세포에 대해 증식 억제효과를 보여주었지만, 특히 꼭지 부위는 최고농도에서 60.3%의 높은 증식 억제효과를 보였다. 그러나 마우스 유래의 간암세포에 대한 활성보다 인체유래 간암세포에 대한 활성이 낮게 나타났다. 참외의 꼭지 추출물에서 QR 유도활성과 항암활성을 확인함으로써 향후 참외 비가식 부위의 기능성 소재로의 이용화에 대한 연구가 필요할 것으로 생각된다.

• 생물환경조절학회지
• /
• 제13권4호
• /
• pp.185-193
• /
• 2004

배의 과경부에 지베렐린을 처리하면 과실비대에 효과는 있으나 저장성이 떨어지는 단점이 있다. 본 시험은 지베렐린 처리가 저장성을 향상시키기 위하여 폴리아민의 처리부위와 처리농도에 따른 과실의 특성변화를 조사하였다. 시험품종은 '황금배', '영산배', '추황배'로 하여 제베렐린을 만개 30일 후에 처리하고 폴리아민을 수확 7일 전에 과총 엽과 수관에 100, 200, 500ppm으로 엽면살포 하였다. 지베렐린 처리에 의하여 과중은 모든 품종에서 $10\%$ 정도 커졌고 경도는 $0.3\~0.4kg/\phi5mm$만큼 낮아졌으나 당도와 식미는 차이가 없었다. 과경부의 형태적 특성은 지베렐린 처리가 무처리에 비하여 수(髓, pith) 부분에서 내피세포까지의 거리와 내피에서 표피세포까지의 거리가평균 $42.0\%$신장되어 양 수분의 이동이 활발해지고 그에 따른 공급량이 많아져 과실의 무게가 비대해 진 것으로 추정할 수 있었다. 저장 30일 후 과실의 특성을 보면 '황금배'는 과총엽 살포의 스퍼미딘 200ppm이, 수관살포는 스퍼미딘 500ppm에서 과육의 경도가 높게 유지되어 식미가 양호하였다. '영산배'와 '추황배'도 비슷한 경향으로 주당 살포량이 적은 과총엽 200ppm살포처리가 저장성 증진에 효과적이었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권6호
• /
• pp.816-824
• /
• 2006

This study was conducted to evaluate the shoot height at which the yield and nutritive value of wormwood (Artemisia montana) is optimized in order to provide information on its potential to support animal production (Experiment 1). A second objective was to determine how the essential oil (EO) concentration in wormwood hay and silage differ (Experiment 2). In Experiment 1, Artemisia montana was harvested at five different shoot heights (20, 40, 60, 80 and 100 cm) from triplicate $1.8{\times}1.8m$ plots. Dry matter (DM) yield was measured at each harvest date and the harvested wormwood was botanically separated into leaf, stalk and whole plant fractions and analyzed for chemical composition and in vitro dry matter digestibility (DMD). Values for total digestible nutrients (TDN), digestible energy (DE) and metabolizable energy (ME) were subsequently calculated using prediction equations. Dry matter yields of stalk and whole plant increased linearly (p<0.001) and leaf yield increased quadratically (p<0.01) with shoot height, whereas the leaf/stalk ratio decreased linearly (p<0.001). As shoot height increased, there was a linear increase (p<0.001) in leaf DM, ether extract (EE) and neutral detergent fiber (NDF) contents and a quadratic increase (p<0.05) in leaf acid detergent fiber (ADF) and nitrogen free extract (NFE) contents, and stalk and whole plant DM (p<0.001), organic matter (OM, p<0.01 and p<0.05), NDF (p<0.001 and 0.05) and NFE (p<0.05) contents. However, there were decreases in leaf crude protein content (CP, quadratic, p<0.001) and stalk and whole plant EE content (linear, p<0.001), CP (quadratic, p<0.05) and ash (quadratic, p<0.05) contents. Digestibility of DM and TDN, and DE and ME value in leaves were not affected by increasing shoot height, but these measures linearly decreased (p<0.001) in stalk and whole plant. In Experiment 2, the hay had higher DM and CP concentrations, but lower EE concentration than the silage. Essential oil (EO) content in wormwood silage (0.49 g/100 g DM) was higher (p<0.05) than that in wormwood hay (0.32 g/100 g DM). Wormwood hay contained 25 essentail oils (EO) including camphor (10.4 g/100 g), 1-borneol (11.6 g/100 g) and caryophyllene oxide (27.7 g/100 g), and wormwood silage had 26 EO constituents including 3-cyclohexen-1-ol (8.1 g/100 g), trans-caryophyllene (8.6 g/100 g) and ${\gamma}$-selinene (16.8 g/100 g). It is concluded that the most ideal shoot height for harvesting wormwood is 60 cm based on the optimization of DM yield and nutritive value. Wormwood silage had a greater quantity and array of EO than wormwood hay.

• 화훼연구
• /
• 제16권1호
• /
• pp.44-48
• /
• 2008

호접란 촉성재배를 위한 하계 고온기 폐광 자연냉풍처리가 개화에 미치는 영향을 밝히고자 본 실험을 실시하여 다음과 같은 결과를 얻었다. 보령지방 폐광의 자연냉풍온도는 $12{\sim}14^{\circ}C$이였으며 이 온도를 주온 $22^{\circ}C$, 야온 $20^{\circ}C$로 조절하여 6월부터 8월까지 처리하였다. 이 온도는 호접란 화성유도 기본 온도범위이었으며, 이 때 외부 평균기온은 $28.4{\sim}32.8^{\circ}C$로 나타났다. 화경 발생은 20일저온경과에서 3% 발생되었으며, 45일 경과에서 65% 이상 발생되었고, 90일 경과에서 100% 발생하였다. 화경장은 30일 경과에서 48.7cm로 짧았으며, 60일 경과에서 62.4cm로 길어져 처리기간이 길수록 화경장이 신장되었다. 화수장, 소화수도 같은 경향이었고, 화경 마디수는 6~7개로 저온 경과일수와는 큰 영향이 없는 것으로 나타났다. 개화는 45일 경과에서 9월 15일에 가장 빨리 개화되었고 저온 경과일수가 길수록 늦어지는 경향을 나타내었다. 고온기 폐광 자연냉풍처리는 무처리에 비하여 화경 발생시기는 3개월, 개화기는 4개월 정도 촉진되었다.

• 한국연초학회지
• /
• 제18권1호
• /
• pp.54-59
• /
• 1996

Erwinia carotovora subsp. Carotovora (Ecc), a pathogen of tobacco hollow stalk disease, was isolated for testing susceptibility to streptomycin from diseased plants in burley tobacco growing area. Of 157 isolates tested, 17 isolates (108%) were resistant to the antibiotic at the antibiotic from field soils, which streptomycin had been used continuously for three years for control of the disease was three times higher than those of non-used. There was no difference in virulence and generation time between streptomycin-sensitive and resistant strains.

• 한국연초학회지
• /
• 제3권2호
• /
• pp.131-133
• /
• 1981

The hollow stalk, caused by Erwinia carotovors var. carotovora, is probaly the most devastating disease to burley tobacco in Korea. There are two modes of infection to tobacco plants: the primary infection along the plant stem from soil and the secondary one from the topped end of plants. The secondary infection rate could be reduced to almost zero by applying an antibiotic, streptomycin sulfate(400 ppm solution), right after topping.

• The Plant Pathology Journal
• /
• 제30권4호
• /
• pp.397-406
• /
• 2014

Maize is a socioeconomically important crop in many countries. Recently, a high incidence of stalk rot disease has been reported in several maize fields in Gangwon province. In this report, we show that maize stalk rot is associated with the fungal pathogens Fusarium subglutinans and F. temperatum. Since no fungicides are available to control these pathogens on maize plants, we selected six fungicides (tebuconazole, difenoconazole, fluquinconazole, azoxystrobin, prochloraz and kresoxim-methyl) and examined their effectiveness against the two pathogens. The in vitro antifungal effects of the six fungicides on mycelial growth and colony formation were investigated. Based on the inhibition of mycelial growth, the most toxic fungicide was tebuconazole with 50% effective concentrations ($EC_{50}$) of < $0.1{\mu}g/ml$ and $EC_{90}$ values of $0.9{\mu}g/ml$ for both pathogens, while the least toxic fungicide was azoxystrobin with $EC_{50}$ values of 0.7 and $0.5{\mu}g/ml$ for F. subglutinans and F. temperatum, respectively, and $EC_{90}$ values of > $3,000{\mu}g/ml$ for both pathogens. Based on the inhibition of colony formation by the two pathogens, kresoxim-methyl was the most toxic fungicide with complete inhibition of colony formation at concentrations of 0.1 and $0.01{\mu}g/ml$ for F. subglutinans and F. temperatum, respectively, whereas azoxystrobin was the least toxic fungicide with complete inhibition of colony formation at concentrations > $3,000{\mu}g/ml$ for both pathogens.

• 한국연초학회지
• /
• 제14권2호
• /
• pp.126-132
• /
• 1992

This experiment was conducted to examine estimates of heterosis, degree of dominance, heritability and phenotypic and genotypic correlation for several mutant characters in flue-cured tobacco variety. Significant heterosis was noted, recording 2.73 and 6.16% for leaf width, -6.86 and -4.72 for leaf shape index, 19.8 and 23.4% for bacterial wilt disease index in Fl an4 F2 generation, respectively, and -1.44 for days to flower in F2 generation. And also leaf width was appeared partial dominance in Fl, and leaf shape index in Fl and F2, Leaf width in F2 was appeared overdominance. Estimated heritabilities in the broad sense ranged from 0.77 to 0.87 for plant and stalk height and days to flower, while those of leaf number, leaf length and width, leaf shape index and bacterial wilt disease index ranged from 0.50 to 0.65. And the heritabilities in the narrow sense were appeared 0.64 and 0.72 for stalk and plant height, respectively, and the others were ranged from 0.32 to 0.47 Positive genotpic correlations appeared among plant height, stalk height, leaf length and leaf width, and between leaf shape index ands day to flower. And negative genotypic correlations appeared between leaf shape index and plant height and stalk height and leaf width, and between bacterial wilt disease index and leaf length.

• 펄프종이기술
• /
• 제38권3호
• /
• pp.47-60
• /
• 2006

This study was carried out to investigate the sheet formation properties of Morus Hanjis, made of bast and whole stalk pulps by different pulping methods, such as alkali, alkali-peroxide and sulfomethylated pulping. Two species of Morus, M. alba and M. lhou, were used. Effect of morphological properties of pulp stocks on the sheet formation and its gray levels based on optical property were evaluated using an Image analyzer. In addition, the effect of fiber distribution index(FDI) which was calculated from tile data of Confocal laser scanning microscopy(CLSM) on the sheet formation and optical properties of Morus Hanji were also discussed. On the sheet formation, Hanji from whole stalk pulp was superior than that of bast pulp. The more the sheet formation improved, the more paper opacity decreased. In the aspect of Hanji's surface characteristics analyzed by an Image analyzer, the average gray level and its standard deviation of Hanji from the whole stalk pulp were rather lower than those of bast pulp because of better sheet formation of the former. However, high brightness Hanji showed high value of gray level. The sheet formation and paper opacity were increased with the decrease of standard deviation of gray level. From these results, gray level measurement could be used to predict the paper opacity as well as sheet formation. Sheet formation of whole stalk Hanji and its FDI measured by CLSM were higher than those of bast fibers. In conclusion, the sheet formation and opacity of Hanji could be evaluated by standard deviation value of Hanji's gray level using an Image analyzer and by fiber distribution index using CLSM.