Browse > Article

Quinone Reductase Inductive Activity and Growth Inhibitory Effect against Hepatoma Cell of Oriental Melon Extract  

Kim, Hye-Suk (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University)
Ku, Kang-Mo (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University)
Suh, Jun-Kyu (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University)
Kang, Young-Hwa (Department of Horticulture, College of Agriculture and Life Science, Kyungpook National University)
Publication Information
Journal of Bio-Environment Control / v.18, no.4, 2009 , pp. 448-453 More about this Journal
Abstract
This study was performed to elucidate anticancer activities of various parts, such as peel, flesh, placenta, seed, stalk and stem leaf of oriental melon. Chemopreventive and anticancer effects of oriental melon extract were evaluated by detoxifying enzyme, quinone reductase (QR) inductive activity, cytotoxicity and growth inhibitory effect against hepatoma cell. Stalk and stem leaf extracts of oriental melon showed the increment of QR inductive activity with dose-dependent manner and induced quinone reductase 3.9, 1.5-fold at $200{\mu}g/mL$ respectively compared to control. The growth inhibitory effect of oriental melon extract against mouse hepatoma cell (Hepa1c1c7) was investigated by crystal violet (CV) assay. Stalk and stem leaf of oriental melon showed potent growth inhibitory effect. Based on these result, the growth inhibitory effects of stalk, stem leaf at various concentration were examined in detail by MTT assay using human hepatoma cancer cell (HepG2). All of two parts showed growth inhibitory effects and expecially stalk exhibited inhibitory effect of 60.3% at maximum concentration. The above results suggest that stalk of oriental melon has a possibility as a source of natural cancer chemopreventive materials.
Keywords
anticancer; chemoprevention; MTT; QR;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Prochaska, H.J., M.J. De Long, and P. Talalay. 1985. On the mechanism of induction of cancer protective enzymes; A unifying proposal. Proc. Natl. Acad. Sci. USA 82(23):2832-2836
2 Kelly, W., X. Wu, and R.H. Liu. 2003. Antioxidant activity of apple peels. J. Agric. Food Chem. 51(3):609-614   DOI   ScienceOn
3 Kim, J.S, Y.J. Nam, and T.W. Kwon. 1996. Induction of quinone reductase activity by genistein, soybean isoflavone. Food Sci. Biotechnol. 5(1):70-75
4 Kim, H.J., M.H. Yu, S.O. Lee, J.H. Park, D.C. Park, and I.S. Lee. 2004. Effects of plum fruits extracts at different growth stages on quinone reductase induction and growth inhibition on cancer cells. J. Korean Soc. Food Sci. Nutr. 33(9):1445-1450   DOI   ScienceOn
5 Kim, Y.E., J.W. Yang, C.H. Lee, and E.K. Kwon. 2009. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake Sing (pine mushroom). J. Korean Soc. Food Sci. Nutr. 35(5):555-560   DOI
6 Martin, A. and M. Clynes. 1993. Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays. Cytotechnology 11(1):49-58   DOI   ScienceOn
7 Park, S.D., Y.S. Shin, S.G. Bae, Y.J. Seo, I.K. Yeon, and H.W. Do. 2004. Colletion of cultivation of oriental melon. Jae-Tak Youn. Seong ju fruit vegetable experiment station. Korea, pp. 7-13
8 Shim, S.M., S.W. Choi, and S.J. Bae. 2001. Effects of punica granatum L. fractions on quinone reductase induction and growth inhibition on serveral cancer cells. J. Kor. Soc. Food Sci. Nutr. 30(1):80-85
9 Wefers, H., T. Komai, P. Talalay, and H. Sies. 1984. Protection against reactive oxygen species by NAD(P)H: quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). Decreased hepatic low-level chemiluminescence during quinone redox cycling. FEBS Lett. 169(1):63-66   DOI   ScienceOn
10 Steinkellner, H., S. Rabot, C. Freywald, E. Nobis, G. Scharf, M. Chabicovsky, S. Knasmuller, and F. Kassie. 2001. Effects of cruciferous vegetables and their constituents on drug metabolizing enzymes involved in the bioactivation of DNA-reactive dietary carcinogens. Mutat. Res. 1:480-481   DOI   ScienceOn
11 Fish, B. 1984. Clinical trials for the evaluation of cancer therapy. Cancer Res. 54: 609-615
12 Talalay, P. and A.M. Benson. 1982. Elevation of quinone reductase activity by anticarcinogenic antioxidants. Adv. Enzyme Reg. 20:287-300   DOI   ScienceOn
13 Kennelly, E.J., C. Gerhauser, L.L. Song, J.G. Graham, C.W.W. Beecher, J.M. Pezzuto, and A.D. Kinghorn. 1997. Induction of quinone reductase by withanolides isolated from Physalis philadelphica (tomatillos). J. Agric. Food Chem. 45:3771-3777   DOI   ScienceOn
14 Yu, M.H., H.J. Lee, H.G. Im, S.O. Lee, and I.S. Lee. 2006. Induction of quinone reductase activity in hepatoma cells by paprika (Capsicum annuum L.). Kor. J. Food Sci. Technol. 38(5):707-711
15 Antonio, F., B. D'abrosca, S. Pacifico, C. Mastellone, M. Scognamiglio, and P. Monaco. 2009. Identification and assessment of antioxidant capacity of phytochemicals from kiwi fruits. J. Agric. Food Chem. 57:4148-4155   DOI   ScienceOn
16 Mehta, R.G., J. Liu, A. Constantinou, C.F. Thomas, M. Hawthorne, M. You, C. Gerhauser, J.M. Pezzuto, R.C. Moon, and R.M. Moriarty. 1995. Cancer chemopreventive activity of brassinin, a phytoalexin from cabbage. Carcinogenesis 16:399-404   DOI   ScienceOn
17 Prochaska, H.J. and A.B. Santamaria. 1998. Direct measurement of NAD(P)H: quinone reductase from cells cultured in microtiter wells: a screening assay for anticarcinogenic enzyme inducers. Anal. Biochem. 169(2):328-36   DOI   ScienceOn
18 Brenner, D.E. 2000. Multiagent chemopreventive agent combinations. J. Cell Biochem. Suppl. 34:121-124   DOI   PUBMED   ScienceOn
19 National Statistical Office. 2008. Annual report on the cause of death statistics. Republic of Korea
20 Talalay, P., J.W. Fahey, W.D. Holtzclaw, T. Prestera, and Y. Zhang. 1995. Chemoprotection against cancer by phase 2 enzyme induction. Toxicol. Lett. 82(83): 173-179   DOI   ScienceOn
21 Drysdale, B.E., C.M. Zacharchuk, M. Okajima, and H.S. Shin. 1986. Assay of a cytocidal protein excreted by activated macrophages. Method. Enzymol. 132: 549-55   DOI
22 Kim, H.Y., K.S. Woo, I.G. Hwang, Y.R. Lee, and H.S. Jeong. 2008. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J. Food Sci. Technol. 40(2):166-170   과학기술학회마을
23 Zhang, Y., T.W. Kensler, C.G. Cho, G.H. Posner, and P. Talalay. 1994. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornyl isothiocyanates. Proc. Natl. Acad. Sci. USA 91(8):3147-3150   DOI   ScienceOn
24 Shin, Y.S., J.E. Lee, I.K.Yeon, H.W. Do, J.D. Cheung, C.K. Kang, S.Y. Choi, S.J. Youn, J.G. Cho, and D.J. Kwoen. 2008. Antioxidant and antimicrobial effects of extract with water and ethanol of oriental melon (Cucumis melo L. var makuwa Makino). J. Kor. Soc. Appl. Biol. Chem. 51(3):194-199
25 Shin, Y.S., J.E. Lee, I.K. Yeon, H.W. Do, J.D. Cheung, C.K. Kang, S.Y. Choi, S.J. Youn, J.G. Cho, and D.J. Kwoen. 2008. Antioxidant effects and tyrosinase inhibition activity of oriental melon (Cucumis melo L. var makuwa Makino) extracts. J. Life Scien. 18(7): 963-967
26 Hwang, I.G., K.S. Woo, T.M. Kim, D.J. Kim, M.H. Yang, and H.S. Jeong. 2006. Change of physicochemical characteristics of korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Kor. J. Food Sci. Technol. 38(3):342-347
27 Kinghorn, A.D., B. Cui, A. Ito, H.S. Chung, E.K. Seo, L. Long, and L.C. Chang. 2000. Fractionation of plants to discover substances to combat cancer. Biologically active natural products. Pharmaceuticals. CRC Press, Washington D.C., USA, pp. 17-24
28 Bae, J.H., S.O. Yu, Y.M. Kim, S.U. Chon, B.W. Kim, and B.G. Heo. 2009. Physiological activity of methanol extracts from Ligularia fischeri and their hyperplasia inhibition ativity of cancer cell. J. Bio-Env. con. 18(1):67-73
29 Hamada, A. 2005. Development of an individualized therapy for establishing the optimal dosage by the pharmacokinetics profiles of anticancer agents. Yakugaku zasshi 125(8):631-637   DOI   PUBMED   ScienceOn
30 Doll, R. 1992. The lessons of life. Cancer Research. 52:2024-2029
31 Sporn, M.B. 1998. Carcinogenesis and cancer. Cancer Res. 51:6215-6218