• Title/Summary/Keyword: stacked

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Crystal Structure of SAV0927 and Its Functional Implications

  • Jeong, Soyeon;Kim, Hyo Jung;Ha, Nam-Chul;Kwon, Ae-Ran
    • Journal of Microbiology and Biotechnology
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    • v.29 no.3
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    • pp.500-505
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    • 2019
  • Staphylococcus aureus is a round-shaped, gram-positive bacterium that can cause numerous infectious diseases ranging from mild infections such as skin infections and food poisoning to life-threatening infections such as sepsis, endocarditis and toxic shock syndrome. Various antibiotic-resistant strains of S. aureus have frequently emerged, threatening human lives significantly. Despite much research on the genetics of S. aureus, many of its genes remain unknown functionally and structurally. To counteract its toxins and to prevent the antibiotic resistance of S. aureus, our understanding of S. aureus should be increased at the proteomic scale. SAV0927 was first sequenced in an antibiotic resistant S. aureus strain. The gene is a conserved hypothetical protein, and its homologues appear to be restricted to Firmicutes. In this study, we determined the crystal structure of SAV0927 at $2.5{\AA}$ resolution. The protein was primarily dimeric both in solution and in the crystals. The asymmetric unit contained five dimers that are stacked linearly with ${\sim}80^{\circ}$ rotation by each dimer, and these interactions further continued in the crystal packing, resulting in a long linear polymer. The crystal structures, together with the network analysis, provide functional implications for the SAV0927-mediated protein network.

Searching for LSB Dwarf Satellite Galaxies Around Nearby Galaxies in IMSNG Data

  • Lim, Gu;Im, Myungshin;Kim, Jisu;Lee, Jeong Hwan;Choi, Changsu;Ehgamberdiev, S.;Burkhonov, O.;Mirzaqulov, D.
    • The Bulletin of The Korean Astronomical Society
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    • v.44 no.1
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    • pp.75.2-75.2
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    • 2019
  • Low surface brightness (LSB) dwarf galaxies hold a key to resolve the small-scale problems of Lambda Cold Dark Matter (LCDM) paradigm such as missing satellites problem. Many recent studies found LSB dwarf galaxies around massive galaxies beyond Local Group up to 10Mpc. Motivated by this, we can increase the number of them by searching for LSB dwarf galaxies around galaxies up to 40Mpc. We use stacked deep (${\mu}_R{\sim}26.2mag\;arcsec^{-2}$) optical B, R-band images taken from Maidanak 1.5m telescope, one of facilities of Intensive Monitoring Survey of Nearby Galaxies (IMSNG) which monitored nearby galaxies in a day cadence from 2014 to 2016. Extended LSB sources in ambient regions of 16 nearby galaxies are selected using central surface brightness and total R magnitude criteria. After that, 24 LSB dwarf candidates are selected with visual inspection. To identify if the candidates are satellites or not, we are trying to compare the number density of LSB dwarf candidates around massive galaxies with those in Canada-France-Hawaii-Telescope Legacy Survey (CFHTLS) wide fields which have no dominant massive galaxies for control sample.

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Wrinkling of Graphene Papers Placed on Stretchable Adhesive Films (신축성 접착 필름 위에 놓인 그래핀 종이의 주름 생성)

  • Kim, Sang-Yun;Jeong, Myeong Hee;Suk, Ji Won
    • Composites Research
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    • v.34 no.2
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    • pp.108-114
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    • 2021
  • Graphene flakes are generally mass-produced by converting graphene oxide into reduced graphene oxide using chemical or thermal reduction. These graphene flakes can be stacked to form a free-standing graphene paper, which can be used for various applications. However, a graphene paper lacks stretchability, which hinders its application in stretchable devices. In this work, we introduced wrinkles in a graphene paper to make it stretchable. A graphene paper fabricated by vacuum-filtering a graphene dispersion was placed on a pre-stretched adhesive film. When the pre-stretched adhesive film returned to the original state, the graphene paper was wrinkled. The effect of the pre-stretching and wet condition of the graphene papers was experimentally investigated by using scanning electron microscopy. In addition, we observed the change of the period of the wrinkles in the graphene paper depending on the pre-stretching.

Design of Multipliers Optimized for CNN Inference Accelerators (CNN 추론 연산 가속기를 위한 곱셈기 최적화 설계)

  • Lee, Jae-Woo;Lee, Jaesung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.25 no.10
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    • pp.1403-1408
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    • 2021
  • Recently, FPGA-based AI processors are being studied actively. Deep convolutional neural networks (CNN) are basic computational structures performed by AI processors and require a very large amount of multiplication. Considering that the multiplication coefficients used in CNN inference operation are all constants and that an FPGA is easy to design a multiplier tailored to a specific coefficient, this paper proposes a methodology to optimize the multiplier. The method utilizes 2's complement and distributive law to minimize the number of bits with a value of 1 in a multiplication coefficient, and thereby reduces the number of required stacked adders. As a result of applying this method to the actual example of implementing CNN in FPGA, the logic usage is reduced by up to 30.2% and the propagation delay is also reduced by up to 22%. Even when implemented with an ASIC chip, the hardware area is reduced by up to 35% and the delay is reduced by up to 19.2%.

Josephson effect of the superconducting van der Waals junction

  • Park, Sungyu;Kwon, Chang Il;Kim, Jun Sung
    • Progress in Superconductivity and Cryogenics
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    • v.23 no.2
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    • pp.6-9
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    • 2021
  • Heterostructures fabricated by various combinations of van der Waals (vdW) materials enable us to investigate disorder-free physical properties and realize novel functional devices. Superconducting vdW junctions have attracted a lot of attention because of its simple structure without a barrier layer. In superconducting vdW junction, without extra fabrication effort, a natural barrier can be formed, whose character is sensitive to distance and angle of lattice between two superconducting vdW materials. Using high-quality single crystals and the dry transfer technique, we fabricated the vertically stacked NbSe2/NbSe2 and FeSe/FeSe vdW junctions and investigated their Josephson junction properties. We found that in the FeSe junctions, Josephson coupling is extremely sensitive to the fabrication conditions, in contrast to the NbSe2 junctions. We attributed this distinct character of the FeSe junctions to surface instability and small Fermi surface of FeSe.

Application of black phosphorus nanodots to live cell imaging

  • Shin, Yong Cheol;Song, Su-Jin;Lee, Yu Bin;Kang, Moon Sung;Lee, Hyun Uk;Oh, Jin-Woo;Han, Dong-Wook
    • Biomaterials Research
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    • v.22 no.4
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    • pp.352-359
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    • 2018
  • Background: Black phosphorus (BP) has emerged as a novel class of nanomaterials owing to its unique optical and electronic properties. BP, a two-dimensional (2D) nanomaterial, is a structure where phosphorenes are stacked together in layers by van der Waals interactions. However, although BP nanodots have many advantages, their biosafety and biological effect have not yet been elucidated as compared to the other nanomaterials. Therefore, it is particularly important to assess the cytotoxicity of BP nanodots for exploring their potentials as novel biomaterials. Methods: BP nanodots were prepared by exfoliation with a modified ultrasonication-assisted solution method. The physicochemical properties of BP nanodots were characterized by transmission electron microscopy, dynamic light scattering, Raman spectroscopy, and X-ray diffractometry. In addition, the cytotoxicity of BP nanodots against C2C12 myoblasts was evaluated. Moreover, their cell imaging potential was investigated. Results: Herein, we concentrated on evaluating the cytotoxicity of BP nanodots and investigating their cell imaging potential. It was revealed that the BP nanodots were cytocompatible at a low concentration, although the cell viability was decreased with increasing BP nanodot concentration. Furthermore, our results demonstrated that the cells took up the BP nanodots, and the BP nanodots exhibited green fluorescence. Conclusions: In conclusion, our findings suggest that the BP nanodots have suitable biocompatibility, and are promising candidates as fluorescence probes for biomedical imaging applications.

A Study on the Mechanical Properties of Duplex Stainless Steel Weldment According to Mo Contents

  • Bae, Seong Han;Lim, Hee Dae;Jung, Won Jung;Gil, Woong;Jeon, Eon Chan;Lee, Sung Geun;Lee, Hyo Jong;Kim, In Soo;Lee, Hae Woo
    • Korean Journal of Metals and Materials
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    • v.50 no.9
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    • pp.645-651
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    • 2012
  • This study investigated changes in phase fraction caused by the addition of Mo, as well as the subsequent behaviour of N and its effect on the mechanical properties of welded 24Cr-N duplex stainless steel weld metals. Filler metal was produced by fixing the contents of Cr, Ni, N, and Mn while adjusting the Mo content to 1.4, 2.5, 3.5 wt%. The delta ferrite fraction increased as the Mo content increased. In contrast, the ${\gamma}$ fraction decreased and changed from a round to an acicular shape. Secondary austenite (${\gamma}^{\prime}$) was observed in all specimens in a refined form, but it decreased as the Mo content increased to the extent that it was nearly impossible to find any secondary austenite at 3.5 wt% Mo. Both tensile and yield strengths increased with the addition of Mo. In contrast, the highest value of ductility was observed at 1.41 wt% Mo. At all temperatures, impact energy absorption showed the lowest value at 3.5 wt% Mo, at which the amount of ${\delta}$-ferrite was greatest. There was no significant temperature dependence of the impact energy absorption values for any of the specimens. As the fraction of ${\gamma}$ phase decreased, the amount of N stacked in the ${\gamma}$ phase increased. Consequently, the stacking fault energy decreased, while the hardness of ${\gamma}$ increased.

Analysis of Impact on ERP Customization Module Using CSR Data

  • Yoo, Byung-Keun;Kim, Seung-Hee
    • Journal of Information Processing Systems
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    • v.17 no.3
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    • pp.473-488
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    • 2021
  • The enterprise resource planning (ERP) system is a standardized and advanced business process that many companies are implementing now-a-days through customization. However, it affects the efficiency of operations as these customizations are based on uniqueness. In this study, we analyzed the impact of customized modules and processing time on customer service request (CSR), by utilizing the stacked CSR data during the construction and operation of ERP, focusing on small and medium-sized enterprises (SMEs). As a result, a positive correlation was found between unit companies and the length of ERP implementation; ERP modules and the length of ERP implementation; ERP modules and unit companies; and the type of ERP implementation and ERP module. In terms of CSR, a comparison of CSR processing time of CBO (customized business object) module and STD (standard) module revealed that while the five modules did not display statistically significant differences, one module demonstrated a statistically very significant difference. In sum, the analysis indicates that the CBO-type CSR and its processing cost are higher than those of STD-type CSR. These results indicate that companies planning to implement an ERP system should consider the ERP module and their customization ratio and level. It not only gives the theoretical validity that should be considered as an indicator for decision making when ERP is constructed, but also its implications on the impact of processing time suggesting that the maintenance costs and project scheduling of ERP software must also be considered. This study is the first to present the degree of impact on the operation and maintenance of customized modules based on actual data and can provide a theoretical basis for applying SW change ratio in the cost estimation of ERP system maintenance.

Thoracodorsal artery flaps for breast reconstruction-the variants and its approach

  • Thomsen, Jorn Bo;Rindom, Mikkel Borsen;Rancati, Alberto;Angrigiani, Claudio
    • Archives of Plastic Surgery
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    • v.48 no.1
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    • pp.15-25
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    • 2021
  • Thoracodorsal artery (TDA) flaps, ranging from the vascular-pedicled thoracodorsal artery perforator (TDAP) flap, the propeller TDAP flap, and the muscle-sparing latissimus dorsi (MSLD) flap to the conventional latissimus dorsi (LD) flap and the extended LD flap, can all be used for breast reconstruction. The aim of this paper and review is to share our experiences and recommendations for procedure selection when applying TDA-based flaps for breast reconstruction. We describe the different surgical techniques and our thoughts and experience regarding indications and selection between these procedures for individual patients who opt for breast reconstruction. We have performed 574 TDA flaps in 491 patients: 60 extended LD flaps, 122 conventional LD flaps, two MSLD flaps, 233 propeller TDAP flaps, 122 TDAP flaps, and 35 free contralateral TDAP flaps for stacked TDAP breast reconstruction. All the TDA flaps are important flaps for reconstruction of the breast. The LD flap is still an option, although we prefer flaps without muscle when possible. The vascular-pedicled TDAP flap is an option for experienced surgeons, and the propeller TDAP flap can be used in most reconstructive cases of the breast, although a secondary procedure is often necessary for correction of the pedicle bulk. The extended LD flap is an option for women with a substantial body mass index, although it is associated with the highest morbidity of all the TDA flaps. The MSLD flap can be used if the perforators are small or if dissection of the perforators is assessed to be hazardous.

Study of Simultaneous Analysis of Indicator Components of DTP(Digital Textile Printing) Textile Products Using HPLC-MS/MS - Focusing on Natural Dyes - (HPLC-MS/MS를 활용한 DTP(Digital Textile Printing) 제품의 지표성분 동시분석법 연구 - 천연염료를 중심으로 -)

  • Lee, Wonkyoung;Sung, Eunji;Moon, Joung Ryul;Kim, Miji;Kim, Jonghoon
    • Fashion & Textile Research Journal
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    • v.23 no.6
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    • pp.844-851
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    • 2021
  • Due to the increase in consumers' interest about well-being, interest in eco-friendly products has been increasing due to the harmful effects of various harmful substances contained in textile products and environmental issues. As a result, natural dyes of less potential risk than synthetic dyes and digital textile printing(DTP) textile products with less environmental pollution are drawing attention. However, due to the lack of evaluation criteria for DTP textile products with natural ink and the nature of many colors are stacked layer by layer for dying, the need for simultaneous analysis is emerging. To evaluate whether the natural dye is derived from natural ingredients, the biocarbon content is analyzed. However, in the case of ink made using natural dyes and DTP textile products using natural ink, it is difficult to analyze the biocarbon content due to the limitation of the presence of a small amount of dye contained therein. In this study, we were shown the possibility of natural derived verification by cross-checking the analytes of natural dyes (Persicaria tinctoria, an indigo dye; Dactylopius coccus, a light red; and Curcum longa L., i.e., turmeric) and natural ink with HPLC-MS/MS. The coefficient of determination was 0.999 or higher, the limit of quantification was 0.647-3.664 ㎍/L and a %RSD of each indicator material was less than 10. Then, the extraction amount of natural dyes for five patterned fabrics was analyzed.