• 생명과학회지
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• 제19권3호
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• pp.305-310
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• 2009

플라스미드 pGKV21에는 229-nt single-strand DNA initiation (ssi) signal이 존재한다. Asymmetric PCR 기법으로 합성된 229-nt ssDNA 단편을 이용하여 실제로 RNA polymerase에 의한 priming ability와 protein interaction을 확인하였다. in vitro primer RNA 합성 실험 결과, 229-nt ssDNA 단편은 filamentous M13 phage의 주형 DNA에서와 비슷한 효율로 시발체 RNA를 합성하였으며, 이 반응은 strand-specific하게 이루어졌다. DNase I footprinting과 gel retardation 실험 결과, RNA polymerase와 SSB 단백질은 229-nt ssDNA 단편에 stable interaction을 하며, 시발체 RNA를 합성하였다. 또한, in vivo 조건 하에서 RNA polymerase의 저해제인 rifampicin을 처리하여 세포내에 ssDNA 중간체가 집적되는 정도를 비교하여 본 결과, 플라스미드 pGKV21은 rifampicin-sensitive RNA polymerase가 상보가닥 합성에 관여 함을 보여 주었다.

• 전기학회논문지
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• 제57권5호
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• pp.797-802
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• 2008

High Speed Rail Train Control System consists of CTC, IXL and ATC. IXL and ATC perform train control and command via interchanging relevant information between a signal room and CTC. However, it is proved that IXL and ATC are attributed to train delay error since those systems are highly sensitive to trackside conditions. Especially, network error on IXL blocks transmitting signal information to adjacent signal room so that its effects give rise to system overall problems. In order to figure out the measures for which minimizing the occurrence rate of train delay error due to HSR TCS, This paper is performed analysis on communication network structure, the length of SSI network roof and SSI-TFM distance by examining and analyzing the error cases related to IXL in a network aspect.

• BMB Reports
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• 제28권4호
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• pp.336-341
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• 1995

Using a mutant M13 phage derivative lacking a great part of the complementary strand synthesis origin, we identified six single-strand initiation (ssi) signals for DNA replication in pACYC184, pLG214, pGKV21, and pDPT270 plasmids, and named them $ssiA_{YC}$, $ssiA_{LG}$, $ssiB_{LG}$, $ssiA_{KV}$, $ssiA_{PT}$, and $ssiB_{PT}$, respectively. Two of them were from pDPT270, one from downstream the on of pACYC184, two from pLG214, one from upstream the plus origin of pGKV21. Introduction of these ssi signals into the deleted $ori_c$ site of a mutant filamentous M13 phage ($M13{\Delta}lac182$) resulted in the restoration of growth activity of this phage. These ssi signals were classified into a number of groups on the basis of sequence similarity. $ssiA_{YC}$ and $ssiA_{LG}$ show extensive sequence homology to the n'-site (primosome assembly sites) of ColE1, whereas $ssiB_{PT}$ is homologous to the n'-site of ${\Phi}X174$. $ssiA_{PT}$ belongs to G4-type ssi signals which require only dnaG primase and SSB protein for the priming of replication. In addition, possible biological roles of these ssi signals are discussed.

• Smart Structures and Systems
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• 제10권4_5호
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• pp.331-351
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• 2012

In this study an output-only system identification technique for civil structures under ambient vibrations is carried out, mainly focused on using the Stochastic Subspace Identification (SSI) based algorithms. A newly developed signal processing technique, called Singular Spectrum Analysis (SSA), capable to smooth a noisy signal, is adopted for preprocessing the measurement data. An SSA-based SSI algorithm with the aim of finding accurate and true modal parameters is developed through stabilization diagram which is constructed by plotting the identified system poles with increasing the size of data matrix. First, comparative study between different approaches, with and without using SSA to pre-process the data, on determining the model order and selecting the true system poles is examined in this study through numerical simulation. Finally, application of the proposed system identification task to the real large scale structure: Canton Tower, a benchmark problem for structural health monitoring of high-rise slender structures, using SSA-based SSI algorithm is carried out to extract the dynamic characteristics of the tower from output-only measurements.

• 대한전기학회논문지:시스템및제어부문D
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• 제49권1호
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• pp.48-53
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• 2000

In this paper, we develop a TMS320C31 (60MHz) digital signal processor (DSP) board to synthesize gradient waveforms for Spiral Scan Imaging (SSI), which is one of the ultra fast magnetic resonance imaging (MRI) methods widely used. In SSI, accurate gradient waveforms are very essential to high quality magnetic resonance images. For this purpose, sampling rate for synthesizing the gradient waveforms is set twice as high as the data sampling rate. With the developed DSP boards accurate gradient waveforms are obtained. Ultra fast spiral scan imaging with the developed with the developed DSP board is currently under development.

• Smart Structures and Systems
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• 제29권3호
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• pp.499-512
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• 2022

This work proposes a novel contactless vibration damping and thermal isolation tripod platform based on Superconducting Magnetic Levitation (SML). This prototype is suitable for cryogenic environments, where classical passive, semi active and active vibration isolation techniques may present tribological problems due to the low temperatures and/or cannot guarantee an enough thermal isolation. The levitating platform consists of a Superconducting Magnetic Levitation (SML) with inherent passive static stabilization. In addition, the use of Operational Modal Analysis (OMA) technique is proposed to characterize the transmissibility function from the baseplate to the platform. The OMA is based on the Stochastic Subspace Identification (SSI) by using the Expectation Maximization (EM) algorithm. This paper contributes to the use of SSI-EM for SML applications by proposing a step-by-step experimental methodology to process the measured data, which are obtained with different unknown excitations: ambient excitation and impulse excitation. Thus, the performance of SSI-EM for SML applications can be improved, providing a good estimation of the natural frequency and damping ratio without any controlled excitation, which is the main obstacle to use an experimental modal analysis in cryogenic environments. The dynamic response of the 510 g levitating platform has been characterized by means of OMA in a cryogenic, 77 K, and high vacuum, 1E-5 mbar, environment. The measured vertical and radial stiffness are 9872.4 N/m and 21329 N/m, respectively, whilst the measured vertical and radial damping values are 0.5278 Nm/s and 0.8938 Nm/s. The first natural frequency in vertical direction has been identified to be 27.39 Hz, whilst a value of 40.26 Hz was identified for the radial direction. The determined damping values for both modes are 0.46% and 0.53%, respectively.

• 융합신호처리학회논문지
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• 제24권1호
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• pp.1-14
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• 2023

Underwater images carry information that is useful in the fields of aquaculture, underwater military security, navigation, transportation, and so on. In this research, we transmitted an underwater image through various underwater mediums in the presence of underwater turbulence and beam attenuation effects using a high-speed visible optical carrier signal. The optical beam undergoes scintillation because of the turbulence and attenuation effects; therefore, distorted images were observed at the receiver end. To understand the behavior of the communication media, we obtained the bit error rate (BER) performance of the system with respect to the average signal-to-noise ratio (SNR). Also, the structural similarity index (SSI) and peak SNR (PSNR) metrics of the received image were evaluated. Based on the received images, we employed suitable nonlinear filters to recover the distorted images and enhance them further. The BER, SSI, and PSNR metrics of the specific nonlinear filters were also evaluated and compared with the unfiltered metrics. These metrics were evaluated using the on-off keying and binary phase-shift keying modulation techniques for the 50-m and 100-m links for beam attenuation resulting from pure seawater, clear ocean water, and coastal ocean water mediums.

• 한국음향학회지
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• 제40권2호
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• pp.161-168
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• 2021

본 논문에서는 발성하고 있는 입 주변에 40 kHz의 주파수를 갖는 초음파 신호를 방사하고 되돌아오는 신호의 도플러 변이를 검출하여 발성음을 합성하는 무 음성 대화기술을 제안하였다. 무음성 대화 기술에서는 비 음성 신호로 부터 추출된 특징변수와 해당 음성 신호의 파라메터 간 대응 규칙을 생성하고 이를 이용하여 음성신호를 합성하게 된다. 기존의 무 음성 대화기술에서는 추정된 음성 파라메터와 실제 음성 파라메터간의 오차가 최소화되도록 대응규칙을 생성한다. 본 연구에서는 추정 음성 파라메터가 실제 음성 파라메터의 분포와 유사하도록 생성적 적대 신경망을 도입하여 대응 규칙을 생성하도록 하였다. 60개 한국어 음성을 대상으로 한 실험에서 제안된 기법은 객관적, 주관적 지표상 으로 기존의 신경망 기반 기법보다 우수한 성능을 나타내었다.

• 융합신호처리학회 학술대회논문집
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• 한국신호처리시스템학회 2000년도 추계종합학술대회논문집
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• pp.25-28
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• 2000

시변 특성을 갖는 무선 채널 환경에서 UEP(Unequal Error Protection) 채널 부호화 방법은 효율적인 정보 전송을 가능케 한다. 본 논문에서는 IP-SCCC (Interleaved Puncturing Serially Concatenated Convolutional Codes)로 CSI(Channel State Information)와 SSI(Source Significance Information)를 이용한 두가지 UEP 채널 부호화 방법을 제안한다. 또한 AWGN(Additive White Gaussian Noise)과 Rayleigh 페이딩이 존재하는 채널을 가정하고 모의 실험을 통해 알고리즘의 우수성을 검증한다.

• 전자공학회논문지
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• 제53권12호
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• pp.131-138
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• 2016

본 연구에서는 CT에서 사용되는 요오드화 조영제가 가돌리늄조영제와 비교하여 MR영상신호에 어떠한 영향을 미치는지 알아보고자 하였으며, 이에 따른 CT조영증강 검사 이후 MRI검사를 시행하는 프로토콜이 적정한지에 대하여 논하고자 하였다. 실험은 iodine과 gadolinium의 두 개의 팬텀을 제작하여 MRI에서의 통상적인 T1, T2, T2 FLAIR, 3D Angio 검사를 시행 후, 이에 대한 정량적 분석이 이루어졌다. 실험결과 체내 자유수(Free water)와 유사한 셀라인의 신호강도 SSI(Saline's Signal Intensity)는 iodine팬텀에서 각 175, 1231, 333, 37 [a.u]을 보였고, gadolinium팬텀에서 101, 1021, 321, 31 [a.u]을 기록하였다. 셀라인의 SI(Signal Intensity)를 기준으로 가장 큰 차이의 신호강도 BDEPS(the Biggest Difference of EPS)는 iodine팬텀에서 각 1297, 123, 757, 232 [a.u]를 보였고, gadolinium팬텀에서 793, 6, 1495, 365 [a.u]를 기록하였다. 이때 셀라인과 비교한 신호증강정도 EPS(Enhancement Percentage to Saline)는 iodine팬텀에서 641.1 -90.0, 127.3, 527%를 보였고, gadolinium팬텀에서 685.1, 99.4, 365.7, 1077.4% 기록하였다. BDEPS를 보이는 지점인 BP(BDEPS's point)는 iodine팬텀에서 900, 900, 477, 900 mmol을 보였고, gadolinium팬텀에서 4, 0.2, 0.2, 40 mmol을 기록하였다. 셀라인과 비교 하여 육안으로 SI변화를 확인할 수 있는 지점 CPSS(Change Point of SI to SSI)는 iodine팬텀에서 63, 423, 63, 29 mmol을 보였고, gadolinium팬텀에서 각 [50, 30], [4, 0.2], [4, 1], 0.2 mmol을 기록하였다. 본 연구를 통하여 iodine 역시 MR신호에 영향을 끼치며, 이는 gadolinium과는 다른 패턴을 보이는 것을 확인하였다. 이에 따라 임상현장에서 본 연구의 정량화 데이터를 감안하여 CT와 MRI 검사 순서 프로토콜을 결정한다면 유용한 진단학적 MR영상을 구현 할 수 있을 것으로 사료된다.