• Journal of Advanced Marine Engineering and Technology
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• v.36 no.6
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• pp.802-807
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• 2012

Self-piercing rivet is sheet joining method. It is being used more to join aluminum alloy sheets. Self-piercing riveting is a large-deformation process that involves piercing. The self-piercing rivet, under the press from the punch, pierces the top sheet and forms a mechanical interlock with the bottom sheet. In this study, forging process was designed for manufacturing self-piercing rivet. The forging process has been simulated by using commercial FEM code DEFORM-2D. In simulation of forging process for manufacturing rivet, process sequence, formability, forging load, and distributions of stress and strain were investigated. The suitable forging process could be designed by comparisons of simulation results. The developed process consists of four stages: upsetting, first chamfering, back extrusion, and second chamfering. The simulated results for forging process were confirmed by experimental trials with the same conditions.

• KSBB Journal
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• v.28 no.1
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• pp.54-59
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• 2013

Three isolates, E. faecium P1, P2 and P3, from intestinal drugs of three phamaceutical companies, four clinical vancomycin resistant isolates, E. faecium V1, V2, V3 and E. faecalis V4, and three isolates, E. faecalis DW01, DW07 and DW14, from infant feces were tested for the presence of virulence genes, ace, agg, esp, efaA, gelE, sprE, vanA and vanB as well as fsrABC, regulatory genes of gelE and sprE, cylMBA, cytolysin activation genes and cpd, cob and ccf, pheromone genes by PCR and for their phenotype activities such as protease, biofilm formation, cell clumping and hemolysis. The genes encoding cell surface adherence proteins, ace, agg, esp and efaA, were predominantly amplified from the vancomycin resistant strain V4 and the fecal isolates DW01, DW07 and DW14. Both protease and biofilm formation activity were detected only from E. faecalis V4 from which the PCR products of gelE and spreE as well as fsrABC were amplified. The pheromone genes were amplified from the V4, DW01, DW07 and DW14 strains and these strains showed clumping activity. Biofilm formation was observed from the strains DW01, DW07 and DW14, all of which produced PCR products of pheromone, and V4 as well. Whole cytolysin regulator genes were amplified from DW01, DW07 and DW14 and ${\beta}$-hemolysis activity was detected from these strains. Any virulence genes or activities except the pheomone gene ccf were not detected from the pharmaceutical isolates, E. faecium P1, P2 and P3.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.17 no.1
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• pp.1-10
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• 2004

Adaptive finite element analysis, in which its solution error meets with the user defined allowable error, is recently used to improve the reliability of finite element analysis results. This adaptive analysis is composed of two procedures; one is the error estimation of an analysis result and the other is the reconstruction of finite elements. In the (p-method, an element size is controlled by relocating of nodal positions (r-method) and the order of an element shape function is determined by the hierarchical polynomial (p-method) corresponding to the clement solution error by the enhanced SPR. In order to show the effectiveness and the accuracy of the suggested rp-method, various numerical examples were analyzed and these analysis results were examined by comparing with those obtained by the existed methods.

• Journal of Power System Engineering
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• v.19 no.3
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• pp.11-15
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• 2015

In this paper, the multi-point hydrogen detection system based on the combination of the hetero-core optical fiber SPR hydrogen tip sensor and interrogator by pseudorandom noise (PN) code correlation reflectometry has been developed. In a light intensity-based experiment with an LED operating at 850 nm, it has been presented that a transmitted loss change of 0.32dB was induced with a response time of 25 s for 4% $H_2$ in $N_2$ in the case of the 25-nm Au, 60-nm $Ta_2O_5$, and 5-nm Pd multi-layers film. The proposed sensor characteristic shows excellent reproducibility in terms of loss level and time response for the in- and out- $H_2$ action. In addition, in the experiment for multi-point hydrogen detection, all sensors show the real-time response for 4% hydrogen adding with reproducible working. As a result, the real-time multi-point hydrogen detection could be realized by means of the combination of interrogating system and hetero-core optical fiber SPR hydrogen tip sensors.

• International Journal of Aeronautical and Space Sciences
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• v.10 no.2
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• pp.86-94
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• 2009

This paper deals with the creation of a new, low-cost point/position tracking system that can measure deformations in engineering structures with simple commercially widespread cameras. Though point tracking systems do exist today, such as Stereo Pattern Recognition (SPR) and Projection Moir$\acute{e}$ Interferometry (PMI) systems, they are far too costly to use to analyze small, simple structures because complex optical components such as large flashes, high-resolution cameras and data acquisition systems with several computers are required. We developed a point tracking system using commercial cameras. This system used IR LEDs and commercial IR CCD cameras to minimize the interference posed by other extraneous light sources. The main algorithm used for this system is an optical point tracking algorithm, which is composed of the point extraction algorithm and the point matching algorithm for 3-D motion estimation. a series of verification tests were performed. Then, the developed point tracking system was applied to measure deformations of an acrylic plate under a mechanical load. The measured deformations of the acrylic plate matched well with the numerical analysis results. The results indicate that the developed point tracking system is reliable enough to measure continuous deformed shapes of various engineering structures.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.1
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• pp.75-85
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• 2023

This study was conducted to assess the effect of heptapeptide, composed of seven amino acids, on the activation of human hair cells isolated from human hair follicles. We have confirmed that the heptapeptide could bind to Lgr5 from the results of surface plasmon resonance (SPR) analysis. Heptapeptide enhanced the proliferation of human hair follicle dermal papilla cells (HHFDPCs) in a dose dependent manner. It induced the protein level of nuclear β-catenin, and the expressions of β-catenin downstream target genes, including LEF1, Cyc-D1 and c-Myc, in HHFDPCs. Heptapeptide significantly induced the phosphorylation of Akt and ERK, and the mRNA expressions of growth factors, including hepatocyte growth factor (HGF), keratinocyte growth factor (KGF) and vascular endothelial growth factor (VEGF), in HHFDPCs. In addition, heptapeptide significantly increased mRNA expression levels of differentiation-related transcription factors of human hair germinal matrix cells (HHGMCs) and differentiation markers of human hair outer root sheath cells (HHORSCs). Additionally, we investigated the effect of heptapeptide on human hair follicle stem cells (HHFSCs) differentiation and found that the heptapeptide reduced the mRNA and protein levels of stem cell markers, while it increased those levels of differentiation markers. These results have indicated that the heptapeptide promotes proliferation or differentiation of various types of hair follicle constituent cells through the induction of Wnt/β-catenin signaling. From the results, we have suggested that the heptapeptide in this study could be applied as a new functional material for the improvement of hair growth and alopecia.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.52 no.3
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• pp.148-155
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• 2003

In this paper, a nonlinear anisotropic filtering method without the loss of important information happened due to the repeated filtering in magnetic resonance images is proposed. First of all original images are divided into four regions, e.g., SPR(Strong Plain Region), EPR(Easy Plain Region), SER(Strong Edge Region), and EER(Easy Edge Region). An optimal template among multiple templates is selected, then the nonlinear anisotropic filtering based on the template is applied in pixel by pixel basis. In the proposed algorithm, filtering strength of EER containing important information is adjusted very weak and filtering strength for remaining regions is also adjusted according to the degree of the importance. In spite of repeated filtering, resulting images by the proposed method could still preserve anatomy information of original images without any degradation. Compared to the existing nonlinear anisotropic filtering, the proposed filtering method with multiple templates provides higher reliability for filtered images.

• Asian Journal of Atmospheric Environment
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• v.6 no.1
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• pp.33-40
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• 2012

In this study, we characterized the physical form of allergenic Cry j 1 in the urban atmosphere. Through an immunofluorescence antibody method, we showed that allergenic Cry j 1 exists as fine particles (${\leq}1.1{\mu}m$). To determine Cry j 1 concentrations and its particle size distribution, we used the ELISA method to confirm that most Cry j 1 exists as fine particles in the urban atmosphere and is found at high concentrations on fine day next to rainy day. Furthermore, we evaluated Cry j 1 denaturation by using the Biacore J system based on the surface plasmon resonence (SPR) principle and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). We showed that the dissociation constant ($K_D$) of Cry j 1 that has been exposed to urban polluted air is lower ($1.76{\times}10^{-14}$ M) than that of Cry j 1 ($1.32{\times}10^{-9}-3.37{\times}10^{-9}$ M) of original pollen grains that has not been exposed to air pollutants. Cry j 1 turns into low molecular weight proteins by reacting with various acidic solutions. In sum, we showed that allergenic Cry j 1 exists as fine particles that can deposit in the lower respiratory tract. This finding clarifies the relationship between Japanese cedar pollinosis and air pollutants.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.145-152
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• 2008

A cell-based in vitro exposure system was developed to determine whether oxidative stress plays a role in the cytotoxic effects of volatile organic compounds (VOCs) such as benzene, toluene, xylene, and chlorobenzene, using human epithelial HeLa cells. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for immobilization of the HeLa cells on a gold (Au) substrate. In addition, an immobilized cell-based sensor was applied to the electrochemical detection of the VOCs. Layer formation and immobilization of the cells were investigated with surface plasmon resonance (SPR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The adhered living cells were exposed to VOCs; this caused a change in the SPR angle and the VOC-specific electrochemical signal. In addition, VOC toxicity was found to correlate with the degree of nitric oxide (NO) generation and EIS. The primary reason for the marked increase in impedance was the change of aqueous electrolyte composition as a result of cell responses. The p53 and NF-${\kappa}B $ downregulation were closely related to the magnitude of growth inhibition associated with increasing concentrations of each VOC. Therefore, the proposed cell immobilization method, using a self-assembly technique and VOC-specific electrochemical signals, can be applied to construct a cell microarray for onsite VOC monitoring.

• Proceedings of the Membrane Society of Korea Conference
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• 2004.05a
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• pp.65-68
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• 2004

Molecular imprinting, which was first proposed by Wulff and Sarhan in 1972 [1], is a facile way to construct molecular recognition sites by applying a simple radical polymerization [2]. Since 1994, the authors have proposed an alternative molecular imprinting method in which polymeric materials are directly converted into molecular recognition materials [3].(omitted)