• The Plant Pathology Journal
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• v.20 no.4
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• pp.313-315
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• 2004

The coat protein gene of Korean isolate of Ricer stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a protein of 322 amino acids. RSV-Kr showed 94%-99% sequence identities to that of Japanese- and Chinese isolates.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.651-655
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• 2000

Polymerase chain reaction (PCR) was used to specifically detect Phytophthora infestans by analyzing the sequences of the ribosomal internal transcribed spacer regions (ITS) in the rDNA of the Phytophthora species. Based on the sequence data, PISP-1 together with the ITS3 primer were used to detect p. infestans. A single ca. 450 bp segment was observed in P. infestans, but not in the other fungal or bacterial isolates. Two factors, the annealing temperature and template DNA quantity, were investigated to determine the optimal conditions. Using these species-specific primers, a unique band was obtained within annealing temperatures of $55^{\circ}C$-$61^{\circ}C$ and template DNA levels of 10 pg-100 ng.

• Proceedings of the Korean Reliability Society Conference
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• 2000.11a
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• pp.269-277
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• 2000

The component reliability database is required in PSA (Probabilistic Safety Analysis) for NPP (Nuclear Power Plant). We have applied a generic database to the PSA for the Korean NPPs, since there is no specific component reliability database. Therefore we are developing the plant-specific component reliability database for domestic NPPs. We also extend the experience and knowledge of PSA and component reliability database for NPP to chemical industry We collect the raw data like component operation history and maintenance history and then input the required data for the component reliability database through failure analysis. With the database, we can not only perform PSA with real data but also perform maintenance optimization.

• Korean journal of applied entomology
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• v.14 no.2 s.23
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• pp.47-52
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• 1975

Helminthosporium vicotoriae and H. carbonum were grown under fluorescent plus incandescent lights, or in darkness, with several different temperature regimes. There was little or no effect of light on toxin production by H. victoriae. Light-grown cultures of H. carbonum had significantly higher titres of host-specific toxin than did dark-grown cultures. Light had no significant effect on growth of either fungus. Maximum titres of host-specific toxins from both fungi were evident at the time maximum growth was reached. Minimum pH values in Fries modified medium occurred at the time of, or slightly before maximum level of toxin was reached.

• Korean Journal Plant Pathology
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• v.14 no.1
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• pp.92-98
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• 1998

RAPD analysis was performed from four host-specific toxin (HST) producing Alternaria, i.e., A. kikuchiana, A. mali, a. longipes and A. Longipes and A. alternata f. sp. lycopersici, nonpathogenic A. alternata and A. brassicicola to assess their phylogenetic relationship. DNA polymorphism was detected among species (pathotypes) of HST producing Alternaria by PCR amplification and differentiation of the species was recognized by RAPD analysis. Primer OPA-02 was the most profitable among 7 notificated primers for differentiation of the HST producing Alternaria species. UPGMA analysis of the RAPD bands from alternaria spp. revealed that HST producing Alternaria and nonpathogenic a. alternata are closely related.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1998.06a
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• pp.50-69
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• 1998

Dimethomorph is a novel fungicide with a high level of activity against diseases induced by certain Oomycetes, including fungal populations that are resistant to other products. In several ways, this fungicide illustrates the opportunities and challenges presented by many modern pesticides. The specific mode of action, which affects cell wall formation, is associated with a very high level of performance and low dose rates under field conditions. These low dose rates, combined with a low level of toxicity to non-target organisms present an outstanding safety profile. This same highly-specific mode of action, however, limits the spectrum of activity and suggests the need for a resistance management plan, both of which must be addressed in new product development. In addition, the biological and physiochemical properties of this, and other new products are not adequately described by the traditional classification of fungicides into“protectant”and“systemic”types. These unique profiles provide novel and useful products for disease control.

• Korean Journal of Plant Tissue Culture
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• v.28 no.3
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• pp.147-151
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• 2001

Explants of Lactuce sativa cultivar, chungchima, were co-cultivated with Agrobacterium tumefaciences LBA4404, EHA101 strains containing nptll gene and ferritin gene encoding iron storage protein from soybean for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 MS basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were tested with PCR analysis using nptll, ferritin specific primers whether ferritin gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot showed that transcripts of ferritin gene were detected in mature leaf of the transgenic lines. These results suggest that ferritin gene be successfully integrated and transcribed in the putative transgenic lettuce plants.

• Nuclear Engineering and Technology
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• v.15 no.1
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• pp.41-49
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• 1983

A computer code, BERD (Bayesian Estimation of Reliability Data), has been developed and tested in order to update the data for the reliability analysis of safety related systems in a specific nuclear power plant. The code has been used to derive the plant-specific data for reliability analysis of the auxiliary feedwater system of a pressurized water reactor. The prior information for components selected was taken from the U.S. Reactor Safety Study, WASH-1400, and the operating experiences from published licensee event reports. The results show that the updated data are well fitted to log-normal distribution curves and the error factors are reduced significantly.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.181-181
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• 2005

• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1441-1447
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• 2006

The light perception and phototransformation of phytochromes are the first process of the phytochrome-mediated light signal transduction. The chromophore ligation and its photochromism of various site-specific and deletion mutants of pea phytochrome A and bacterial phytochrome-like protein (Cph1) were analyzed in vitro. Serial truncation mutants from the N-terminus and C-terminus indicated that the minimal N-terminal domain for the chromophore ligation spans from the residue 78 to 399 of pea phytochrome A. Site-specific mutants indicated that several residues are critical for the chromophore ligation and/or photochromism. Histidine-324 appears to serve as an anchimeric residue for photochromism through its H-bonding function. Isoleucine-80 and arginine-383 playa critical role for the chromophore ligation and photochromism. Arginine-383 is presumably involved in the stabilization of the Pfr form of pea phytochrome A. Apparently, the amphiphilic ${\alpha}$-helix centered around the residue-391 is in the chromophore pocket and critical for the chromophore ligation.