• 한국식품영양과학회지
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• 제21권6호
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• pp.681-685
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• 1992

돼지고기에서 분리한 bacteriocin 생성능이 우수한 젖산균을 동정하기 위하여 이미 알려진 몇가지 젖산균을 참고균으로 하여 10% denaturing polyacrylamide gel에 전기영동으로 전개한 5S rRNA 와 tRNA 등 150개 이하의 핵산으로 구성된 저분자량 RNA(Low Molecular Weight RNA : LMW RNA) profiles에 나타난 15~25개의 밴드 양상이 균에 따라 차이점을 보여 동정에 효과적으로 이용할 수 있었다. 새로 분리한 젖산균은 참고균과는 다른 균종이었다. APT, TSB, MRS의 3종류 다른 배지에서 배양하여도 LMW RNA profiles에는 차이가 없었으며, 겔상의 밴드 전개거리를 3개의 표준 분자량 물질을 이용하여 만든 표준곡선을 사용하여 상대핵산단위(relative nucleotide units : RNU)로 나타낸 밴드의 양상을 도표화 하는 것이 profiles를 서로 비교 하는데 편리하였다.

• Journal of Ecology and Environment
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• 제46권4호
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• pp.304-315
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• 2022

Background: Filter-feeding zooplankton has limited food resources owing to their habitat. Consequently, it is crucial for them to acquire all essential compounds, such as fatty acids (FAs) and amino acids, from confined diets. To elucidate the trophic transfer of FAs to filter feeders, the primary consumers in freshwater ecosystems, we compared the FA accumulation patterns of two species of filter-feeding zooplankton, Daphnia magna and Branchinella kugenumaensis, in a laboratory experiment. Experimental neonates and nauplii preyed on a single phytoplankton species (Selenastrum capricornutum) for three days after hatching prior to diet switching. Five replicates per feeding group in each species were fed on six different types of mixed phytoplankton diet for 10 days after diet switching. Subsequently, the consumers and diets were harvested and FAs were extracted. Results: Principal component analysis showed that the FA profiles of zooplankton were well-grouped by species and diet. Although diet affects the FA profiles of consumers, they exhibit different FA accumulation patterns. D. magna had a higher 18C-ω3 content and ω3/ω6 ratio than did B. kugenumaensis. In contrast, B. kugenumaensis had higher contents of 18:1ω7 and 20:5ω3 (eicosapentaenoic acid), 22:6ω3 (docosahexaenoic acid), and a higher ratio of ∑18C monounsaturated FAs to ∑18C-ω3 polyunsaturated FAs than did D. magna. Conclusions: This study showed that two primary consumers, D. magna and B. kugenumaensis, fed the same diet had different assimilation patterns of FAs under controlled environments. Specific FA accumulation patterns in filter feeders can provide information on the transfer process of various FAs to high-trophic organisms.

• Transactions on Electrical and Electronic Materials
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• 제14권2호
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• pp.94-100
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• 2013

The elements B, P and As can each be implanted in silicon; for the fabrication of integrated semiconductor devices and the wells in CMOS (complementary metal oxide semiconductor). The implanted range due to different implanted species calculated using TRIM (Transport of Ions in Matter) simulation results was considered. The profiles of implanted samples could be measured using SIMS (secondary ion mass spectrometry). In the comparison between the measured and simulated data, some deviations were shown in the profiles of MeV implanted silicon. The Moliere, C-Kr, and ZBL potentials were used for the range calculations, and the results showed almost no change in the MeV energy region. However, the calculations showed remarkably improved results through the modification of the electronic stopping power. The results also matched very well with SIMS data. The calculated tolerances of $R_p$ and ${\Delta}R_p$ between the modified $S_e$ of TRIM and SIMS data were remarkably better than the tolerances between the TRIM and SIMS data.

• 한국식품영양과학회지
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• 제16권4호
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• pp.337-342
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• 1987

Triglyceride 조성(組成)과 췌장리파제에 의한 PN별(別) 획분(劃分)의 ${\beta}$위치(位置)의 지방산조성(脂肪酸組成)의 결과로부터, 산정가능(算定可能)한 홍화유의 triglyceride 분자종은 23종류였다. 10% 이상을 차지하는 triglyceride분자종은 $C_{18:1}\;C_{18:2}\;C_{18:2}\;OLL;\;13.5%,\;C_{16:0}\;C_{18:2}\;C_{18:2}\;PLL;\;15.0%,\;C_{18:2}\;C_{18:2}\;C_{18:2}\;LLL;\;50.6%$로서 전체의 79.1%이었다.

• Fisheries and Aquatic Sciences
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• 제11권1호
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• pp.32-35
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• 2008

The Suminoe oyster, Crassostrea ariakensis, occurs in estuaries where rivers meet seawater. In Korea, it is one of the most popular fisheries resources in the Nam River and Sumjin River. However, the genetic identification of this species has been questioned, because specimens are often mis-identified as other species. To identify the species, we conducted polymerase chain reaction (PCR) amplification of the internal transcribed spacer-1 (ITS-1) region, followed by digestion with the restriction enzyme HaeIII. Restriction profiles for oysters collected from Korea, Japan, and China (north and south) were determined by comparing the PCR-restriction fragment length polymorphism (RFLP) patterns of the ITS-1 regions. Our study verified that the oysters collected from Korea were C. ariakensis based on the PCR-RFLP patterns. These results emphasize the value of molecular markers for identifying morphologically uncertain species.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.710-712
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• 2003

A new species of Gram-negative halophilic cartenoid producing bacterium was isolated from the Haeundae Coast, Korea. This strain is non-motile, aerobic, orange-pigmented, rod-shaped, and produced carotenoids, mainly astaxanthin. All the type strains of the genus Paracoccus were compared with this strain using 16S rDNA sequence analysis, fatty acid patterns, and physiological reaction profiles. From the results obtained, this strain is classified as a new species, Paracoccus sp..

• Nutrition Research and Practice
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• 제9권1호
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• pp.49-56
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• 2015

BACKGROUND/OBJECTIVES: Glutathione S-transferase (GST) forms a multigene family of phase II detoxification enzymes which are involved in the detoxification of reactive oxygen species. This study examines whether daily supplementation of kale juice can modulate blood pressure (BP), levels of lipid profiles, and blood glucose, and whether this modulation could be affected by the GSTM1 and GSTT1 polymorphisms. SUBJECTS/METHODS: 84 subclinical hypertensive patients showing systolic BP over 130 mmHg or diastolic BP over 85 mmHg received 300 ml/day of kale juice for 6 weeks, and blood samples were collected on 0-week and 6-week in order to evaluate plasma lipid profiles (total cholesterol, triglyceride, HDL-cholesterol, and LDL-cholesterol) and blood glucose. RESULTS: Systolic and diastolic blood pressure was significantly decreased in all patients regardless of their GSTM1 or GSTT1 polymorphisms after kale juice supplementation. Blood glucose level was decreased only in the GSTM1-present genotype, and plasma lipid profiles showed no difference in both the GSTM1-null and GSTM1-present genotypes. In the case of GSTT1, on the other hand, plasma HDL-C was increased and LDL-C was decreased only in the GSTT1-present type, while blood glucose was decreased only in the GSTT1-null genotype. CONCLUSIONS: These findings suggest that the supplementation of kale juice affected blood pressure, lipid profiles, and blood glucose in subclinical hypertensive patients depending on their GST genetic polymorphisms, and the improvement of lipid profiles was mainly greater in the GSTT1-present genotype and the decrease of blood glucose was greater in the GSTM1-present or GSTT1-null genotypes.

• 한국균학회지
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• 제40권2호
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• pp.78-85
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• 2012

20 mer의 URP primers 가 한국 재래적미의 반복배열 DNA 염기서열로부터 고안되어 다양한 곰팡이 종의 PCR 다형성검출에 적용 되어 왔다. URP-PCR 방법은 PCR반응중 $55^{\circ}C$이상의 고온에서 annealing반응을 함으로 하여 재생적인 PCR결과를 얻을 수 있으며 효모균류에서 담자균류의 고등 균류까지 33속, 142 종, 1,489 균주의 다양한 곰팡이 종의 유전체 DNA에 적용되어 그 유용성이 평가 되어 왔다. 본 논문은 URP-PCR의 특성과 지금까지 다양한 곰팡이 종 다양성 검정결과를 종합하여 보고 한다.

• 제어로봇시스템학회논문지
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• 제11권10호
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• pp.830-836
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• 2005

Application of microarray technologies which monitor simultaneously the expression pattern of thousands of individual genes in different biological systems results in a tremendous increase of the amount of available gene expression data and have provided new insights into gene expression during drug development, within disease processes, and across species. There is a great need of data mining methods allowing straightforward interpretation, visualization and analysis of the relevant information contained in gene expression profiles. Specially, classifying biological samples into known classes or phenotypes is an important practical application for microarray gene expression profiles. Gene expression profiles obtained from tissue samples of patients thus allowcancer classification. In this research, molecular classification of microarray gene expression data is applied for multi-class cancer using computational biology such gene selection, principal component analysis and fuzzy clustering. The proposed method was applied to microarray data from leukemia patients; specifically, it was used to interpret the gene expression pattern and analyze the leukemia subtype whose expression profiles correlated with four cases of acute leukemia gene expression. A basic understanding of the microarray data analysis is also introduced.

• 한국동물위생학회지
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• 제20권1호
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• pp.37-45
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• 1997

The result of API staph-ident system was compared with cellular fatty acid composition for the identification of Staphylococcus species isolated from cattle. Isolated strains from cattle were correctly identified to S aureus, S intermedius, S hyicus, S simulans, S saprophyticus, S epidemis, S sciuri and S xylosus by API staph-ident system. The correlation between bacterial cellular fatty acid profile and Staphylococcus species isolated to API STAPH-IDENT system were. In conclusion, the result presented indicate that Staphylococci can be indentified to the species level by the cellular fatty acid profiles. Moreover, computerized fatty acid profile correlative anaylsis can be applied for determining identify of Staphylococcus species.