Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Identification of Korean Suminoe Oyster (Crassostrea ariakensis) by RFLP Analysis

  • Kim, Mi-Jung (Biotechnology Research Institute, National Fisheries Research and Development Institute) ;
  • Park, Jung-Youn (Biotechnology Research Institute, National Fisheries Research and Development Institute) ;
  • Allen, Stanish K. (Virginia Institute of Marine Science, College of William and Mary) ;
  • An, Hye-Suck (Biotechnology Research Institute, National Fisheries Research and Development Institute)
  • Published : 2008.03.31
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Abstract

The Suminoe oyster, Crassostrea ariakensis, occurs in estuaries where rivers meet seawater. In Korea, it is one of the most popular fisheries resources in the Nam River and Sumjin River. However, the genetic identification of this species has been questioned, because specimens are often mis-identified as other species. To identify the species, we conducted polymerase chain reaction (PCR) amplification of the internal transcribed spacer-1 (ITS-1) region, followed by digestion with the restriction enzyme HaeIII. Restriction profiles for oysters collected from Korea, Japan, and China (north and south) were determined by comparing the PCR-restriction fragment length polymorphism (RFLP) patterns of the ITS-1 regions. Our study verified that the oysters collected from Korea were C. ariakensis based on the PCR-RFLP patterns. These results emphasize the value of molecular markers for identifying morphologically uncertain species.

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References

  1. Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci., 62, 727-730 https://doi.org/10.2331/fishsci.62.727
  2. Avise, J.C. 1994. Molecular Markers, Natural History and Evolution. Chapman and Hall, Inc., New York, USA
  3. Avise, J.C., J. Arnold, R.M. Ball, E. Bermingham, T. Lamb, J.E. Neigel, C.A. Reeb and N.C. Saunders. 1987a. Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annu. Rev. Ecol. Syst., 18, 489-522 https://doi.org/10.1146/annurev.es.18.110187.002421
  4. Avise, J.C., C.A. Reeb and N.C. Saunders. 1987b. Geo-graphic population structure and species differences in mitochondrial DNA of mouthbrooding marine cat-fishes (Ariidae) and demersal spawning toadfishes (Batrachoididae). Evolution, 41, 991-1002 https://doi.org/10.2307/2409187
  5. Barkati, S. and R.M. Khan. 1987. Relative growth in three species of oysters. Pak. J. Sci. Ind. Res., 30, 624-627
  6. Boulding, E.G. and T.K. Hay. 1993. Quantitative genetics of shell form of an intertidal snail: constraints on short-term response to selection. Evolution, 47, 576-592 https://doi.org/10.2307/2410072
  7. Cagbn, A.R. 1950. Oyster culture in Japan. Fish. Leaf. Fish. Wild. Serv., 383, 1-80
  8. Cai, Y. and X. Li. 1990. Oyster culture in the People's Republic of China. World Aquacult., 21, 67-72
  9. Carriker, M.R. and P.M. Gaffney. 1996. A catalogue of selected species of living oysters (Ostrea) of the world. In: The Eastern Oyster. Kennedy, V.S., R.E.I. Newell and A.F. Eble, eds. Maryland Sea Grant College Publication, UM-SG-TS-96-01, 1-18
  10. Harry, H.W. 1981. Nominal species of living oysters proposed during the last fifty years. Veliger, 24, 39-45
  11. Hedgecock, D., G. Li, M.A. Banks and Z. Kain. 1999. Occurrence of the Kumamoto oyster Crassostrea sikamea in the Ariake Sea, Japan. Mar. Biol., 133, 65-68 https://doi.org/10.1007/s002270050443
  12. Hillis, D.M. and M.T. Dixon. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Q. Rev. Biol., 66, 411-433 https://doi.org/10.1086/417338
  13. Kuroda, T. and H. Tadashige. 1952. Check List and Bibliography of the Recent Marine Mollusca of Japan. Leo W. Stach Publication, Tokyo, Japan, 1-210
  14. Palumbi, S.R. 1996. What can molecular genetics con-tribute to marine biogeography? An urchin's tale. J. Exp. Mar. Biol. Ecol., 203, 75-92 https://doi.org/10.1016/0022-0981(96)02571-3
  15. Quayle, D.B. and G.F. Newkirk. 1989. Farming bivalve mollusks: methods for study and development. Adv. World Aquacult., 1, 1-294
  16. Rao, K.S. 1987. Taxonomy of Indian Oysters. In: Oyster Culture - Status and Prospects. Nagappan, K. and S. Mahadevan, eds. Central Marine Fisheries Research Institute, India, 1-6
  17. Seed, R. 1968. Factors influencing shell shape in Mytilus edulis, J. Mar. Biol. Ass. U.K., 48, 561-584 https://doi.org/10.1017/S0025315400019159
  18. Tchang, S. and T.K. Lou. 1956. A study on Chinese oysters. Acta Zool. Sinica, 8, 65-93
  19. Torigoe, K. 1981. Oysters in Japan. J. Sci. Hiroshima Univ., Ser. B, 29, 291-319
  20. Zhuang, Q. 1992. Research on marine bivalves in the People's Republic of China. Am. Malacol. Bull., 9, 207-215
  21. Zhang, Q., S.K. Allen Jr and K.S. Reece. 2005. Genetic variation in wild and hatchery stocks of Suminoe oyster (Crassostrea ariakensis) assessed by PCR-RFLP and microsatellite markers. Mar. Biotechnol., 7, 588-599 https://doi.org/10.1007/s10126-004-5105-7
  22. Zhou, M. and S. Allen. 2003. A review of the published work on Crassostrea ariakensis. J. Shellfish Res., 22, 1-20