Browse > Article
http://dx.doi.org/10.5657/fas.2008.11.1.032

Identification of Korean Suminoe Oyster (Crassostrea ariakensis) by RFLP Analysis  

Kim, Mi-Jung (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Park, Jung-Youn (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Allen, Stanish K. (Virginia Institute of Marine Science, College of William and Mary)
An, Hye-Suck (Biotechnology Research Institute, National Fisheries Research and Development Institute)
Publication Information
Fisheries and Aquatic Sciences / v.11, no.1, 2008 , pp. 32-35 More about this Journal
Abstract
The Suminoe oyster, Crassostrea ariakensis, occurs in estuaries where rivers meet seawater. In Korea, it is one of the most popular fisheries resources in the Nam River and Sumjin River. However, the genetic identification of this species has been questioned, because specimens are often mis-identified as other species. To identify the species, we conducted polymerase chain reaction (PCR) amplification of the internal transcribed spacer-1 (ITS-1) region, followed by digestion with the restriction enzyme HaeIII. Restriction profiles for oysters collected from Korea, Japan, and China (north and south) were determined by comparing the PCR-restriction fragment length polymorphism (RFLP) patterns of the ITS-1 regions. Our study verified that the oysters collected from Korea were C. ariakensis based on the PCR-RFLP patterns. These results emphasize the value of molecular markers for identifying morphologically uncertain species.
Keywords
Suminoe oyster; Crassostrea ariakensis; Species identification; RFLP;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci., 62, 727-730   DOI
2 Avise, J.C., J. Arnold, R.M. Ball, E. Bermingham, T. Lamb, J.E. Neigel, C.A. Reeb and N.C. Saunders. 1987a. Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annu. Rev. Ecol. Syst., 18, 489-522   DOI
3 Barkati, S. and R.M. Khan. 1987. Relative growth in three species of oysters. Pak. J. Sci. Ind. Res., 30, 624-627
4 Cagbn, A.R. 1950. Oyster culture in Japan. Fish. Leaf. Fish. Wild. Serv., 383, 1-80
5 Carriker, M.R. and P.M. Gaffney. 1996. A catalogue of selected species of living oysters (Ostrea) of the world. In: The Eastern Oyster. Kennedy, V.S., R.E.I. Newell and A.F. Eble, eds. Maryland Sea Grant College Publication, UM-SG-TS-96-01, 1-18
6 Harry, H.W. 1981. Nominal species of living oysters proposed during the last fifty years. Veliger, 24, 39-45
7 Palumbi, S.R. 1996. What can molecular genetics con-tribute to marine biogeography? An urchin's tale. J. Exp. Mar. Biol. Ecol., 203, 75-92   DOI   ScienceOn
8 Hillis, D.M. and M.T. Dixon. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Q. Rev. Biol., 66, 411-433   DOI   ScienceOn
9 Quayle, D.B. and G.F. Newkirk. 1989. Farming bivalve mollusks: methods for study and development. Adv. World Aquacult., 1, 1-294
10 Tchang, S. and T.K. Lou. 1956. A study on Chinese oysters. Acta Zool. Sinica, 8, 65-93
11 Cai, Y. and X. Li. 1990. Oyster culture in the People's Republic of China. World Aquacult., 21, 67-72
12 Zhuang, Q. 1992. Research on marine bivalves in the People's Republic of China. Am. Malacol. Bull., 9, 207-215
13 Boulding, E.G. and T.K. Hay. 1993. Quantitative genetics of shell form of an intertidal snail: constraints on short-term response to selection. Evolution, 47, 576-592   DOI   ScienceOn
14 Hedgecock, D., G. Li, M.A. Banks and Z. Kain. 1999. Occurrence of the Kumamoto oyster Crassostrea sikamea in the Ariake Sea, Japan. Mar. Biol., 133, 65-68   DOI   ScienceOn
15 Rao, K.S. 1987. Taxonomy of Indian Oysters. In: Oyster Culture - Status and Prospects. Nagappan, K. and S. Mahadevan, eds. Central Marine Fisheries Research Institute, India, 1-6
16 Torigoe, K. 1981. Oysters in Japan. J. Sci. Hiroshima Univ., Ser. B, 29, 291-319
17 Seed, R. 1968. Factors influencing shell shape in Mytilus edulis, J. Mar. Biol. Ass. U.K., 48, 561-584   DOI
18 Avise, J.C. 1994. Molecular Markers, Natural History and Evolution. Chapman and Hall, Inc., New York, USA
19 Avise, J.C., C.A. Reeb and N.C. Saunders. 1987b. Geo-graphic population structure and species differences in mitochondrial DNA of mouthbrooding marine cat-fishes (Ariidae) and demersal spawning toadfishes (Batrachoididae). Evolution, 41, 991-1002   DOI   ScienceOn
20 Zhou, M. and S. Allen. 2003. A review of the published work on Crassostrea ariakensis. J. Shellfish Res., 22, 1-20
21 Kuroda, T. and H. Tadashige. 1952. Check List and Bibliography of the Recent Marine Mollusca of Japan. Leo W. Stach Publication, Tokyo, Japan, 1-210
22 Zhang, Q., S.K. Allen Jr and K.S. Reece. 2005. Genetic variation in wild and hatchery stocks of Suminoe oyster (Crassostrea ariakensis) assessed by PCR-RFLP and microsatellite markers. Mar. Biotechnol., 7, 588-599   DOI