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http://dx.doi.org/10.4489/KJM.2012.40.2.78

Genetic Divesity Analysis of Fungal Species by Universal Rice Primer (URP)-PCR  

Kang, Hee-Wan (Graduate School of Bio.&Information Technology, Hankyong National University)
Publication Information
The Korean Journal of Mycology / v.40, no.2, 2012 , pp. 78-85 More about this Journal
Abstract
URP primers that were derived from repetitive DNA sequence of rice weedy rice have been applied for producing PCR polymorphisms in different fungal species. URP-PCR protocol employed stringent PCR with high annealing temperature over $55^{\circ}C$ throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, Each single URP primer produced characteristic fingerprints from diverse genomes of different fungal species, indicating its universal applicability. URP-PCR has been accessed for applicability to various fungal species with 33 genus, 142 species and 1,489 isolates. Numerous related papers have demonstrated that URP-PCR profiles of fungal species are very useful for identifying fungal species at intra and inter species levels. The results were reviewed in this paper.
Keywords
Fungal species; Genetic diversity; URP-PCR;
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Times Cited By KSCI : 15  (Citation Analysis)
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