• 환경영향평가
• /
• 제18권3호
• /
• pp.161-170
• /
• 2009

As urban growth continues, the earth ecosystem is increasingly dependent on the patterns of urban growth. The impact intensity from urban growth is expected to change predictably with distance from the urban center. However we can't fully understand yet how urban development pattern affects urban ecosystem. In researches about urban ecosystem, it is important to relate the spatial pattern of urbanization to ecological processes. So we used gradient analysis with time data; 1980's, 1990's and 2000's. We attempted to quantify the urban spatiotemporal impacts in Daejeon-city and Cheonan-city, Korea, along a 75km long and 3km wide transect. Through the results, we found the impacts range of urbanization with urban development process of two cities. When the urban growth was concentrated on in both cities, the impacts intensity and range were much stronger and wider. As a result, in urban planning or green space planning, we have to consider suitable urban development forms with surrounding areas, and make legal clauses which limits landuse change. This quantifying the urban gradient is an important step in understanding urban ecology.

• 대한의용생체공학회:의공학회지
• /
• 제27권4호
• /
• pp.170-179
• /
• 2006

The purpose of this study was to investigate the spatiotemporal cortical activation pattern and functional connectivity during visual perception of words. 61 channel recordings of electroencephalogram were obtained from 15 subjects while they were judging the meaning of Korean, English, and Chinese words with concrete meanings. We examined event-related potentials (ERP) and applied independent component analysis (ICA) to find and separate simultaneously activated neural sources. Spectral analysis was also performed to investigate the gamma-band activity (GBA, 30-50 Hz) which is known to reflect feature binding. Five significant ERP components were identified and left hemispheric dominance was observed for most sites. Meaningful differences of amplitudes and latencies among languages were observed. It seemed that familiarity with each language and orthographic characteristics affected the characteristics of ERP components. ICA helped confirm several prominent sources corresponding to some ERP components. The results of spectral and time-frequency analyses showed distinct GBAs at prefrontal, frontal, and temporal sites. The GBAs at prefrontal and temporal sites were significantly correlated with the LPC amplitude and response time. The differences in spatiotemporal patterns of GBA among languages were not prominent compared to the inter-individual differences. The gamma-band coherence revealed short-range connectivity within frontal region and long-range connectivity between frontal, posterior, and temporal sites.

• Molecules and Cells
• /
• 제26권2호
• /
• pp.113-120
• /
• 2008

Laser light microscopy enables observation of various simultaneously occurring events in living cells. This capability is important for monitoring the spatiotemporal patterns of the molecular interactions underlying such events. Two-photon excited fluorescence microscopy (two-photon microscopy), a technology based on multiphoton excitation, is one of the most promising candidates for such imaging. The advantages of two-photon microscopy have spurred wider adoption of the method, especially in neurological studies. Multicolor excitation capability, one advantage of two-photon microscopy, has enabled the quantification of spatiotemporal patterns of $[Ca^{2+}]_i$ and single episodes of fusion pore openings during exocytosis. In pancreatic acinar cells, we have successfully demonstrated the existence of "sequential compound exocytosis" for the first time, a process which has subsequently been identified in a wide variety of secretory cells including exocrine, endocrine and blood cells. Our newly developed method, the two-photon extracellular polar-tracer imaging-based quantification (TEPIQ) method, can be used for determining fusion pores and the diameters of vesicles smaller than the diffraction-limited resolution. Furthermore, two-photon microscopy has the demonstrated capability of obtaining cross-sectional images from deep layers within nearly intact tissue samples over long observation times with excellent spatial resolution. Recently, we have successfully observed a neuron located deeper than 0.9 mm from the brain cortex surface in an anesthetized mouse. This microscopy also enables the monitoring of long-term changes in neural or glial cells in a living mouse. This minireview describes both the current and anticipated capabilities of two-photon microscopy, based on a discussion of previous publications and recently obtained data.

• Molecules and Cells
• /
• 제44권8호
• /
• pp.602-612
• /
• 2021

DNA methylation is an important epigenetic mechanism affecting genome structure, gene regulation, and the silencing of transposable elements. Cell- and tissue-specific methylation patterns are critical for differentiation and development in eukaryotes. Dynamic spatiotemporal methylation data in these cells or tissues is, therefore, of great interest. However, the construction of bisulfite sequencing libraries can be challenging if the starting material is limited or the genome size is small, such as in Arabidopsis. Here, we describe detailed methods for the purification of Arabidopsis embryos at all stages, and the construction of comprehensive bisulfite libraries from small quantities of input. We constructed bisulfite libraries by releasing embryos from intact seeds, using a different approach for each developmental stage, and manually picking single-embryo with microcapillaries. From these libraries, reliable Arabidopsis methylome data were collected allowing, on average, 11-fold coverage of the genome using as few as five globular, heart, and torpedo embryos as raw input material without the need for DNA purification step. On the other hand, purified DNA from as few as eight bending torpedo embryos or a single mature embryo is sufficient for library construction when RNase A is treated before DNA extraction. This method can be broadly applied to cells from different tissues or cells from other model organisms. Methylome construction can be achieved using a minimal amount of input material using our method; thereby, it has the potential to increase our understanding of dynamic spatiotemporal methylation patterns in model organisms.

• 대한물리의학회지
• /
• 제15권1호
• /
• pp.1-9
• /
• 2020

PURPOSE: Many patients with stroke have difficulties in walking with foot-drop. Various types of ankle-foot orthoses (AFOs) have been developed, but their weight needs to be reduced with the assistance of the ankle dorsiflexor. Therefore, an elastic AFO (E-AFO) was devised that not only improves the stability and flexibility of the ankle but also assists with ankle dorsiflexion while walking. This study examined the effects of an E-AFO, on the walking patterns of foot-drop patients with stroke. METHODS: Fourteen patients walked with and without an E-AFO, and the gait parameters were assessed using the GAITRite system. The spatiotemporal data on the gait patterns of stroke patients with foot-drop were compared using paired t-tests; the level of statistical significance was set to α<.05. RESULTS: No significant differences were observed in the velocity (p=.066) and affecte+d step length (p=.980), but the affected and less-affected stance (p=.022, p=.002) and swing time (p=.012, p=.005) were significantly different. The E-AFO produced a significant difference in the less-affected step length (p=.032). CONCLUSION: The E-AFO has a significant effect on the walking patterns of individuals with foot-drop and stroke. The E-AFO could be a useful assistive device for gait training in stroke patients.

• 대한한방내과학회지
• /
• 제41권2호
• /
• pp.194-203
• /
• 2020

Objectives: This study aimed to compare the gait patterns of cerebellar infarction patients with those of corticospinal tract stroke patients through a follow-up of patients with ataxic gait due to cerebellar infarction and corticospinal tract stroke. Methods: We investigated two cases of patients with cerebellar infarction and two cases each of acute or chronic corticospinal tract stroke who were hospitalized at Wonkwang University Gawangju Medical Center from September 1, 2017 to February 15, 2020 based on medical records and gait analyses. The spatiotemporal gait parameters of each patient were measured three times at 2-week intervals except those of the chronic corticospinal tract stroke patients, which were measured twice at a 1-month interval. Results: Spatiotemporal gait parameters, which include velocity, cadence, step length, stride length, and single support, were consistently increased in the cerebellar infarction patients in comparison to the corticospinal tract stroke patients. The stance phase was decreased in all the patients. Conclusions: The cerebellar infarction patients' gait spatiotemporal parameters were found to consistently improve. Moreover, gait analysis can be used to effectively measure improvement of ataxic gait.

• 한국경제지리학회지
• /
• 제22권4호
• /
• pp.458-471
• /
• 2019

DMSP-OLS 야간영상에서 추출된 빛합계지수와 시가지 정보는 사회·경제적 지표 추정과 도시 공간의 역동성 등의 연구에 폭넓게 이용되었다. 본 연구는 1992~2012년 각 연도의 DMSP-OLS 자료를 이용하여 북한과 인접한 중국 지린성 도시들의 경제성장과 시가지 발달의 시·공간적 변화 패턴을 살펴보았다. 지난 20여 년 동안 지린성 빛합계지수 및 각 도시의 빛합계지수는 모두 성장하였다. 또 빛합계지수와 시가지 면적 가중 중심점의 시계열 변화 분석을 한 결과 전자는 북서 방향으로 후자는 남동 방향으로 이동하는 패턴을 따랐다. 이러한 방향성은 중국 정부의 동북진흥전략과 연계된 것으로 추정된다. 향후 연구에서는 DMSP-OLS 자료의 시간적 한계를 극복하기 위한 SNPP VIIRS DNB 영상자료의 공동 활용도 고려되어야 하며, 동시에 경제통계자료와 빛합계지수 간 상관관계에 기반한 회귀모형의 구축을 통해 지역내총생산 등 경제지표의 추정에 관한 연구도 수행되어야 할 것이다.

• 한국지리정보학회지
• /
• 제26권4호
• /
• pp.173-187
• /
• 2023

본격적인 인구감소 시대에 들어선 가운데 지방 도시들은 사회적 인구 유입도 어려운 상황을 고려하여 생활인구 유도를 통한 도시 활력 증진을 모색할 필요가 있다. 본 연구는 이동통신 빅데이터를 활용한 도시활력도 분석을 위해 인구감소 지역인 부산광역시 행정동을 대상으로 주민등록인구와 생활인구의 시공간 분포특성을 비교하였다. 다음으로 행정동을 인구증감의 변화 양상으로 유형화한 후, 도시쇠퇴 및 활력 관련 지표를 사용하여 유형별 지역 특성을 분석하였다. 시공간 분포특성 분석결과, 주민등록인구와 생활인구 밀도 분포패턴은 대체로 비슷한 패턴을 보였으나, 원도심 지역에서 주민등록인구 밀도가 낮으면서 생활인구 밀도가 높은 반대의 특성을 가지는 지역이 나타났다. 주민등록인구와 생활인구의 변화 양상은 상당한 차이를 보였으며, 주민등록인구는 밀도가 낮은 지역에서 인구가 감소하는 반면, 생활인구는 밀도가 높은 지역에서 감소하는 경향을 보였다. 생활인구 감소 군집에 인접해서 인구증감의 지역 간 격자가 큰 군집이 나타나, 인구 밀도가 높은 지역에서 주변 지역에 생활인구가 확산하는 전이효과가 나타날 가능성을 시사했다. 유형화 결과, 주민등록인구가 감소하는 지역에서도 통근·통학 또는 방문으로 인한 활발한 인구 유입이 있었으며, 이러한 지역은 사업체 수의 증가를 유지하고 있어, 산업·경제적 성장이 존재함을 확인하였다. 다만 부산의 약 47% 행정동은 주민등록인구와 생활인구가 모두 감소하고 지역 쇠퇴가 진행되고 있었으며, 이러한 지역에 대해 우선적인 도시 활력 증진을 위한 노력이 필요하다. 본 연구는 교통과 통신의 발달로 인한 도시활동의 한계거리 증가와 이동량의 증가로 지역간 교류가 날로 확대되는 점을 고려해 주민등록인구뿐만 아니라 도시계획지표로서의 생활인구 데이터의 활용 필요성을 제시하였다.

• Journal of Computing Science and Engineering
• /
• 제5권3호
• /
• pp.210-222
• /
• 2011

In this work, we study the problem of privacy-preservation data publishing in moving objects databases. In particular, the trajectory of a mobile user in a plane is no longer a polyline in a two-dimensional space, instead it is a two-dimensional surface of fixed width $2A_{min}$, where $A_{min}$ defines the semi-diameter of the minimum spatial circular extent that must replace the real location of the mobile user on the XY-plane, in the anonymized (kNN) request. The desired anonymity is not achieved and the entire system becomes vulnerable to attackers, since a malicious attacker can observe that during the time, many of the neighbors' ids change, except for a small number of users. Thus, we reinforce the privacy model by clustering the mobile users according to their motion patterns in (u, ${\theta}$) plane, where u and ${\theta}$ define the velocity measure and the motion direction (angle) respectively. In this case, the anonymized (kNN) request looks up neighbors, who belong to the same cluster with the mobile requester in (u, ${\theta}$) space: Thus, we know that the trajectory of the k-anonymous mobile user is within this surface, but we do not know exactly where. We transform the surface's boundary poly-lines to dual points and we focus on the information distortion introduced by this space translation. We develop a set of efficient spatiotemporal access methods and we experimentally measure the impact of information distortion by comparing the performance results of the same spatiotemporal range queries executed on the original database and on the anonymized one.

• Bulletin of the Korean Chemical Society
• /
• 제25권2호
• /
• pp.267-270
• /
• 2004

The bromate-1,4-cyclohexanedione-ferroin oscillating reactions are uncovered to support two types of wave activities, in which spontaneous formation of circular waves has been achieved after the disappearance of initial waves. The induction period of the revival wave is typically above 10 hours and its dependence on the initial concentrations of reactants is qualitatively different from that of initial waves. In addition to their differences in propagating speed and wavelength, the initial waves and the revival patterns have different colors, suggesting that different reaction mechanisms are involved in the formation of these spatiotemporal behaviors. Our experiments further show that the addition of hydroquinone to the reacting system can significantly shorten the induction time of the revival wave, which implicates that hydroquinone is not only a product in the bromate-1,4-cyclohexanedione-ferroin oscillating reaction but also plays a critical role in the following reactions.