• Molecules and Cells
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• 제25권2호
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• pp.247-252
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• 2008

There are several branch points in the flavonoid synthesis pathway starting from chalcone. Among them, the hydroxylation of flavanone is a key step leading to flavonol and anthocyanin. The flavanone 3-${\beta}$-hydroxylase (GmF3H) gene was cloned from soybean (Glycine max cultivar Sinpaldal) and shown to convert eriodictyol and naringenin into taxifolin and dihydrokaempferol, respectively. The major flavonoids in this soybean cultivar were found by LC-MS/MS to be kamepferol O-triglycosides and O-diglycosides. Expression of GmF3H and flavonol synthase (GmFLS) was induced by ultraviolet-B (UV-B) irradiation and their expression stimulated accumulation of kaempferol glycones. Thus, GmF3H and GmFLS appear to be key enzymes in the biosynthesis of the UV-protectant, kaempferol.

• 한국식품영양학회지
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• 제27권2호
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• pp.302-309
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• 2014

In this study, the characteristics of soybeans with different cultivation methods depending on their origins and the properties of tofu made from these soybeans were studied. The isoflavone content was higher in the general Korean soybeans compared to the organic American soybeans. There were no significant differences among the Chinese soybean samples, so the cultivar method was considered to have a greater effect on the isoflavone contents rather than the cultivation method. The protein contents were higher in the tofu made from the general Chinese soybeans and the organic Korean soybeans. Furthermore, the tofu made from the organic Chinese soybeans contained the least amount of proteins. Isoflavone contents were not significantly different among the samples. Texture profile analyses consisting of hardness, cohesiveness, adhesiveness, springiness, and gumminess were all shown to be higher in the organic tofu than in general tofu, with the exception of adhesiveness. In sensory evaluation, color and texture were significantly different according to the samples, and the differences were found to be affected more by the origin of soybeans rather than the cultivation methods. According to this study, the physicochemical properties of the soybeans and tofu seemed to be influenced by the cultivation region rather than the methods.

• Journal of Biosystems Engineering
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• 제37권6호
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• pp.398-403
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• 2012

Purpose: Green soybeans (edamame) are now an economically important and popular food product in Japan. In order to shorten breeding time and to decide an optimal harvest time, we have been developing a dedicated NIRT spectrometer since 2004 for the determination of constituent content such as sucrose and free amino acids, which are two major contributors to the eating quality, in a single pod green soybean. Methods: The obtained models showed that the developed NIRT instrument had reasonable accuracy for the determination of these two components. Then we carried out the investigation into the change in two components during a few days storage using these models with changing time, variety/cultivar, packaging and temperature. Results: The result showed that the most affecting factor on decreasing both sucrose content and free amino acids was variety/cultivar. The time, packaging and temperature also affected significantly in most cases.

• 한국유기농업학회지
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• 제22권4호
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• pp.773-787
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• 2014

Xanthomonas axonopodis pv. glycines에 의해 발생하는 콩 불마름병은 콩을 재배하는 많은 지역에서 가장 많이 발생하는 세균성 병해 중 하나이다. 본 연구는 콩 불마름병 발생 억제를 위한 저항성품종, 파종시기, 작부체계에 대한 효과를 포장에서 평가하기 위하여 수행되었다. 100여 품종을 자연 감염된 포장에서 저항성을 평가한 결과 푸른콩 등 14품종이 저항성을 나타내었고 30여 품종이 병반면적률 3% 이하의 중간 저항성을 나타내었다. 또한 불마름병 감수성 품종인 태광콩을 5월 25일, 6월 5일, 6월 15일, 6월 25일 등 10일 간격으로 파종시기를 조절하여 병반면적률을 조사한 결과 23.3%-25.7%, 14.7%-18.0%, 10.7%-12.8%, 1.0%-2.7%의 발생을 각각 확인하였다. 불마름병 발생이 가장 낮은 처리구는 6월 25일 파종구였으며, 발생이 가장 심한 처리구는 5월 25일 처리구였다. 파종시기가 늦어질수록 불마름병 발생은 감소되는 것을 알 수 있었다. 2006년 12월부터 2007년 6월까지 5개의 콩-동계작물 작부체계의 토양을 채집하여 불마름병 병원균의 생존밀도를 조사하였다. 조사결과 모든 작부체계에서 병원균이 검출되었으며 콩-보리 작부체계와 콩-마늘 작부체계의 병원균 밀도가 다른 작부체계보다 생존밀도가 낮았다. 더욱이 두 개의 작부체계는 콩 불마름병 발생도 감소하는 것으로 확인되었다.

• 한국작물학회지
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• 제34권4호
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• pp.345-352
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• 1989

대두(Glycine max L.) 종자로부터 분리된 렉틴을 토끼에 면역화시켜 렉틴항혈청을 얻었다. 이를 이용한 면역화학적 방법을 사용하여 대두렉팅의 면역화학적 특성과 6개 대두 품종의 종자에서 렉틴의 존재를 조사하였다. Crossed immunoeiectrophoresis한 결과 종자는 4종의 침강선을 보임으로서 렉틴에 4종의 항원성분이 존재함을 보여 주었다. 1％ agarose gel을 이용하여 Immunodouble diffusion test한 결과 6개 대두 공시품종 모두 렉틴항체에 대하여 침강선을 나타내었고 푸종간 침강선은 모두 유사한 양상을 보였다. ELISA 방법에 의하여 6개 대두 품종간의 렉틴함량을 조사, 비교한 결과 렉틴함량은 장엽콩>광교>황금콩>백운콩>장백콩=팔달콩 순으로 나타났다. 공시품종의 조자로부터 분리한 렉틴은 모두 토끼 적혈구는 응집시켰으나 Fusarium sp., Alternaria sp., Cuvalaria sp. 등의 곰팡이 포자는 응집시키지 못하였다.

• 한국작물학회지
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• 제50권4호
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• pp.265-267
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• 2005

Study on effectiveness of Bradyrhizobium japonicum on soybean [Glycine max (L.) Merr.], local cultivar 'Sathiya' were carried out in the garden soil of Trib-huvan University. Different parameters like nodulation, chlorophyll content in fresh leaves and growth of plant in inoculated and uninoculated plant was studied. Pot experiment was conducted in the green house to evaluate the effectiveness of B. japonicum on soybean. It was observed that B. japonicum inoculation increased the number of nodules, shoot length of plant and total chlorophyll content in fresh leaves of soybean plant. However, root length was decreased in all inoculated plants.

• 한국식물병리학회지
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• 제14권3호
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• pp.247-252
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• 1998

A series of greenhouse test was conducted to evaluate infection of Calonectria ilicicola on soybean plants of different ages at time of inoculation. Lesion length and number of perithecia were determined on cultivars Braxton, Deltapine 726, and Riverside 699 that were 10∼40 days old and 4-10 days old at time of inoculation. Quadratic and linear relationships were described between plant age at inoculation and lesion length or perithecia production in greenhouse studies. Soybean seedlings exhibited low susceptibility to C. ilicicola regardless of cultivar susceptibility. On 8- or 1-0-days-old Braxton, lesion lenght and perithecia numbers were reduced. Lesion lengths were longest on plants 30 days old whereas perithecia production was greatest on plants 20∼30 days old at time of inoculation. Differences in lesion length and perithecia production that were observed on young plants (4∼10 days old) were similar to relative levels of susceptibility in soybean cultivars in greenhouse and field tests, suggesting that reaction to C. ilicicola in soybean cultivars may be determined early in plant development.

• 한국작물학회지
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• 제51권7호
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• pp.658-668
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• 2006

SSR 마커를 이용하여 우리나라 콩의 품종판별 기술을 확립하기 위하여 1913년부터 2002년까지 국내에서 육성된 콩 91개 품종에 대하여 5개의 SSR마커(Sat_043, Sat_036, Sat_022, Sat_088 및 Satt045)를 이용하여 판별하였다. 판별용으로 이용된 SSR 5개 마커의 총 대립인자수는 64개이었고, 범위는 $10{\sim}15$개이었으며, 평균 대립인자 수는 12.8개이었다. PIC값은 $0.790(Satt045){\sim}0.905(Sat_043)$의 범위이었으며, 평균 PIC값은 0.857이었다. SSR 마커 5개의 조합으로 5단계의 판별을 통하여 총 91품종 중에서 82품종이 구별되어 약 90%가 판별되었다. 판별 1단계에서 Sat_043으로 판별하였을때 부석의 1품종이, 2단계의 Sat_036으로는 호장콩 등 34품종이, 3단계의 Sat_022로는 단경콩 등 29품종이, 4단계의 Sat_088로는 신팔달콩2호 등 12품종이, 5단계의 Satt045로는 새별콩 등 6품종이 판별되었다. 서로 간에 판별되지 않은 품종들은 형태적 특성에 의하여 구별이 가능하였다.

• 한국작물학회지
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• 제45권4호
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• pp.227-231
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• 2000

Small seed size is one of the major traits of soybean cultivars for sprouts with regard to high sprout yield. This study was conducted to identify quantitative trait loci (QTL) for seed size and weight in a set of F 6 seeds of 89 lines derived from a cross between 'Pureunkong', a soybean cultivar developed for sprouts and 'Jinpumkong 2', a soybean cultivar with no beany taste in seed due to the lack of lipoxygenases. The genetic map of 25 linkage groups with a total of 98 markers including RFLP, RAPD, SSR and classical markers was constructed from this F/sbu 5/-derived population and was used for QTL analysis. 'Pureunkong' was significantly smaller (P＜0.01) than 'Jinpumkong 2' in seed size and seed weight. Genetic variation was detected and transgressive segregation was common in the population for these traits. Seven DNA markers including opT14-1600 in LG A2, opF02-400 in LG B2, Satt100, opC09-700, opG04-730 and opQll-650 in LG C2, and opY07-1100 ＆ 1000 in LG(unknown) were significantly associated and accounted for 4.7 to 10.9% and 5.1 to 10.1 % of the phenotypic variation in seed size and seed weight, respectively. 'Pureunkong' alleles increased seed size and seed weight at the all four significant marker loci on the LG C2. These marker loci in LG C2 were closely linked and were presumed to be a single QTL. Overall, at least three independent QTLs from 3 linkage groups (A2, B2, and C2) were putatively involved in the control of seed size and seed weight.

• Journal of Microbiology
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• 제44권3호
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• pp.363-368
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• 2006

In this study, native Byadyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by Iytic rhizobiophages. Eight indigenous (Soy2, ASR011, ASR031, ASR032, MSR091, ISR050, ISR076 and ISR078) and two exotic strains (USDA123 and CB1809), all of which evidenced a distinct reaction with six phages, were employed in this study. The symbiotic interaction of these strains was studied initially using soybean cultivar JS335 in a sand culture in a controlled environment, and the efficiency was assessed based on the nodule number, nodule dry weight, plant dry weight, nitrogenase activity, and total accumulation of N per plant. Symbiotic effectiveness was found to be highest with the native phage-sensitive isolate ASR011, whereas it was at a minimum with the phage-resistant isolates, ISR050 and ISR078. Additionally, the effectiveness of these strains was evaluated using six soybean cultivars belonging to different maturity groups; namely, Brags, Lee, Pusa20, PK416, JS33S and NRC37. Analysis of variance data evidenced significant differences due to both symbionts, for the majority of the tested parameters. The CB1809, USDA123, and ASR011 strains evidenced relatively superior symbiotic effectiveness with soybean cultivars Brags, Lee and JS335. Strain ISR078 evidenced no significant responses with any of the cultivars. The ASR031 strain performed moderately well with all tested cultivars. The symbiotic response of all the strains was quite poor with cultivar PK416. Our studies showed that a significant relationship existed between the phage sensitivity and symbiotic efficiency of the bacterial strains with the host-cultivars.