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Discrimination of Korean Soybean Cultivars by SSR Markers  

Kim, Seong-Hun (Department of Crop science, Chungbuk National University)
Chung, Jong-Wook (National Institute of Agricultural Biotechnology, RDA)
Moon, Jung-Kyung (National Institute of Crop Science, RDA)
Woo, Seon-Hee (Department of Crop science, Chungbuk National University)
Cho, Yong-Gu (Department of Crop science, Chungbuk National University)
Jong, Seung-Keun (Department of Crop science, Chungbuk National University)
Kim, Hong-Sig (Department of Crop science, Chungbuk National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.51, no.7, 2006 , pp. 658-668 More about this Journal
Abstract
The objective of this study was to develop a technique for the cultivar discrimination using SSR markers in soybean. A total of 91 soybean cultivars developed from 1913 to 2002 in Korea were evaluated by five polymorphic SSR markers (Sat_043, Sat_036, Sat_022, Sat_088 and Satt045). Five SSR markers generated a total of 64 alleles and the number of alleles for each SSR marker ranged from 10 to 15 with average of 12.8. Polymorphic information contents (PIC) by five markers of 91 cultivars were ranged from 0.790 to 0.905 with average of 0.857. A total of 82 cultivars (90%) among 91 soybean cultivars could be individually discriminated by combination of five SSR markers through five step analysis. A cultivar, Buseok, by Sat_043 at the first step, 34 cultivars including Hojangkong by Sat_036 at the second step, 29 cultivars including Dankyeongkong by Sat_022 at the third step, 12 cultivars including Sinpaldalkong 2 by Sat_088 at the fourth step, and 6 cultivars including Saebyeolkong by Satt045 at the fifth step were discriminated. Soybean cultivars which were not discriminated by SSR markers could be discriminated by morphological characteristics.
Keywords
cultivar; discrimination; SSR; soybean;
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