Hyun Jung Lim;Sohyun Park;Joon Ha Lee;In-Woo Kim;HaeYong Kweon;Minchul Seo
International Journal of Industrial Entomology and Biomaterials
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v.47
no.2
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pp.90-98
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2023
The human eye, constantly exposed to solar radiation, can be damaged by UV radiation. In particular, ultraviolet B (UVB)-induced damage plays an important role in retinal degeneration and cell aging. In this study, we investigated the protective effects of the methanol extract of Oxya chinensis sinuosa (OCM), an edible insect known for its high protein content (64.2%), and various pharmacological effects, on human retinal pigment epithelial cells. ARPE-19 cells were treated with OCM and subsequently UVB irradiated. Our results showed that OCM effectively attenuates UVB-induced cell damage by reducing MAPK phosphorylation (JNK and p38 MAPK). Additionally, OCM increased the phosphorylation of Akt, and cell cycle regulators, including p21 and p27, in a dose-dependent manner. Moreover, OCM treatment increased ARPE-19 cell proliferation by activating the S6K1/S6 pathway. This study suggests that OCM prevents UVB-induced retinal cell damage by increasing cell proliferation via ROS reduction, suggesting its potential as a functional therapeutic superfood against retinal cell damage.
KSCE Journal of Civil and Environmental Engineering Research
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v.33
no.1
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pp.317-327
/
2013
Air pollution and artificial heat of urban areas have caused the urban heat island in which asphalt pavements absorb solar heat during the daytime and release the heat at night. Hence, in order to improve the environment of urban areas, it is necessary to examine cooling pavements that can reduce heat on road pavements in urban areas. The application of temperature insulation paints on road pavements require to reduce black brightness for visibility, to increase the reflection rate of infrared light and minimize the reflection rate of visible light. In the study, one part of Acrylic-emulsion was used as a main binder, and the changes in black brightness and the changes of addition ratio (0%, 15%, 30%) of hollow ceramics, as well as kinds of paints (carbon black pigment, mixed mineral pigment) were selected as the main experimental factors. The performance of temperature reduction of cooling pavements was analyzed through the reflection rate of spectrum, the reflection rate of solar heat, and the lamp test. Abrasion resistance, UV accelerated weather resistance, and sliding resistance were tested in real situations. In addition, the performance of heat reduction of testing pavements covered with high-reflection paints was analyzed by using an infrared camera. As the test results, when using mixed mineral paints and hollow ceramic of 30%, the reflection rate of spectrum was 43% in the area of near-infrared ray and 17% in the area of visible light at black brightness of $L^*$=42.89 and the reflection rate of solar heat was 27.5%. Total color difference was ${\Delta}E$=0.27 in the test of UV Accelerated Weather Resistance, indicating almost no changes in color. BPN was more than 53 when scattering #2 and #4 silica sand of more than $0.12kg/m^2$. In Taber's abrasion resistance test, abrasion loss was up to 86.4mg at 500 rotations. The performance of heat reduction was evaluated using an infrared camera at the test section applying high-reflection paints to asphalt pavements, in which the results showed that the temperature was reduced by $12.7^{\circ}C$ on CI-30-40 cooling pavements ($L^*$=38.76) and by $14.2^{\circ}C$ on CI-30-60 cooling pavements ($L^*$=57.12).
Journal of the Society of Cosmetic Scientists of Korea
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v.30
no.2
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pp.227-233
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2004
Chronic exposure to solar radiation, particularly ultraviolet (UV) light, causes a variety of adverse reactions on human skin, such as sunburn, photoaging and photocarcinogenesis. Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental facts play critical roles in cellular damage. And, repeated-UV irradiation activated the expression of the matrix metalloproteinase (MMP) and induced skin irritation. Therefore, the development of effective and safe photoprotectants that can reduce and improve the skin damage has been required. The purpose of this study was to investigate the photo-protective effect of several chinese medical plants (Juniperus chinensis) on the UV -induced skin cell damages. We tested free radical and superoxide scavenging effect in vitro. Fluorometric assays of the proteolytic activities of MMP-1 (collagenase) were performed using fluorescent collagen substrates. UVA induced MMP-1 synthesis and activity were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in skin fibroblasts. We also examined anti-inflammatory effects by the determination test of proinflammatory cytokine, interleukin 6 in HaCaT keratinocytes. Expression of prostaglandin E$_2$ (PGE$_2$) after UVB irradiation was measured by competitive enzyme immunoassay(EIA) using PGE$_2$ monoclonal antibody. In the human skin we tested anti-irritation effect on the SLS-induced damage skin after appling the extract containing emulsion. We found that Juniperus chinensis extract had potent radical scavenging effect by 98% at 100$\mu\textrm{g}$/mL. The extract of Juniperus chinensis showed strong inhibitory effect on MMP-1 activities by 97% at 100 $\mu\textrm{g}$/mL and suppressed the UVA induced expression of MMP-1 by 79% at 25$\mu\textrm{g}$/mL. This extract also showed strong inhibition on MMP-2 activity in UVA irradiated fibroblast by zymography. In the test of proinflammatory cytokines of human keratinocytes Juniperus chinensis extract decreased expression of interleukin 6 about 30%. The amount of PGE$_2$ by HaCaT keratinocytes was significantly increased at the doses of above 10 mJ/$\textrm{cm}^2$ of UVB (p < 0.05). At the concentrations of 3.2-25$\mu\textrm{g}$/mL of this extract, the production of PGE$_2$ by HaCaT keratinocytes (24 h after 10mJ/$\textrm{cm}^2$ UVB irradiation) was significantly inhibited in culture supernatants (p < 0.05). In SLS-induced skin irritation model in vivo, we found to reduce skin erythema and improve barrier recovery after appling Juniperus chinensis extract containing emulsion when compared to irritated non-treated and placebo-treated skin. Our results suggest that Juniperus chinensis extract can be effectively used for the prevention of UV and SLS-induced adverse skin reactions and applied as anti-aging and anti-irritation cosmetics.
In this study, we suggest a facile method to control conditions of single component independently when preparing consisting two-component metal oxides nanofiber by simply dispersing nanoparticles in precursor solution. The well dispersed $SiO_2$ nanoparticles in $TiO_2$ nanofibers were successfully synthesized through a simple electrospinning process. The as-synthesized nanodfibers were investigated via FE-SEM, XRD and EDS for structural studies, furthermore, the analysis of UV-VIS and photocatalytic activity were carried out for demonstrate the effect of $SiO_2$ nanoparticles dispersed in $TiO_2$ nanofibers. As a result, $TiO_2$ nanofibres dispersed with $SiO_2$ nanoparticles have enhanced photocatalytic activity than that of $TiO_2$ nanofibres only. In this strategy, the introduction of $SiO_2$ nanoparticles in $TiO_2$ nanofibers were attribute to enlarge absorption in the visible region (380~440 nm). Additionally, $Br{\o}nsted$ acid sites generated in each metal oxide of Ti and Si increase OH radicals efficiently as well as it limit recombination loss by holding photogenerated electrons for high efficient photocatalytic activity.
Kim, Kwangmin;Kim, Hyungsub;Choi, Dahyun;Lee, Minji;Park, Yunchan;Chu, Wonshik;Chun, Dooman;Lee, Caroline Sunyong
Journal of the Microelectronics and Packaging Society
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v.23
no.2
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pp.65-71
/
2016
In this study, Antimony Tin Oxide (ATO) ion storage layer and $TiO_2$ working electrode were fabricated using Nano Particle Deposition System. NPDS is the cutting-edge technology among the dry deposition methods. Accelerated particles are deposited on the substrate through the nozzle using NPDS. The thicknesses for coated layers were measured and layer's morphology was acquired using SEM. The fabricated electrochromic cell's transmittance was measured using UV-Visible spectrometer and power source at 630 nm. As a result, the integrated electrochromic/DSSC hybrid system was successfully fabricated as an energy harvesting system. The fabricated electrochromic cell was self-operated using DSSC as a power source. In conclusion, the electrochromic cell was operated for 500 cycles, with 49% of maximum transmittance change. Also the photovoltaic efficiency for DSSC was measured to be 2.55% while the electrochromic cell on the integrated system had resulted in 26% of maximum transmittance change.
Journal of the Korean Applied Science and Technology
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v.34
no.1
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pp.50-57
/
2017
Zinc oxide is, one of metal oxide semiconductor, harmless to human and environment-friendly. It has excellent chemical and thermal stability properties. Wurtzite-zinc oxide is a large band gap energy of 3.37 eV and high exciton binding energy of 60 meV. It can be applied to various fields, such as solar cells, degradation of the dye waste, the gas sensor. The photocatalytic activity of zinc oxide is varied according to the particle shape and change of crystallinity. Therefore, It is very important to specify the additives and the experimental variables. In this study, the zinc oxide were synthesized by using a microwave assisted hydrothermal synthesis. The precursor was used as the zinc nitrate, the pH value was controlled as 11 by NaOH. Surfactants are the ethanolamine, cetyltrimethylammonium bromide, sodium dodecyl sulfate, sorbitan monooleate was added by changing the concentration. The composite particles had the shape of a star-like, curcular cone, seed shape, flake-sphere. Physical and chemical properties of the obtained zinc oxide was characterized using x-ray diffractometer, field emission scanning electron microscopy, thermogravimetric analysis and optical properties was characterized using UV-visible spectroscopy, photoluminescence and raman spectroscopy.
Traditional anti-reflective (AR) coating films prepared using tetraethylorthosilicate (TEOS) as a precursor absorbs water easily in addition to having a weak abrasion resistance. To improve the transmittance, hydrophobicity and abrasion resistance of AR coating film, various AR coating films were prepared using methyltrimethoxysilane (MTMS) as a precursor in addition to introducing a fluoroalkylsilane, acid catalyst, base catalyst and acid-base two step catalyst. The prepared AR coating films were then characterized by UV-Vis spectroscopy, contact angle analyzer, atomic force microscope (AFM), pencil scratch hardness test and cross-cut test. As a result, the transmittance of bare glass was 90.5%, while that of AR coating glass increased to 94.8% at curing temperature of $300^{\circ}C$. When the fluoroalkylsilane was added, the water contact angle of AR coating film increased from $96.3^{\circ}$ to $108^{\circ}$, indicating that the hydrophobicity of the film was greatly improved. The abrasion resistance of AR coating film was also improved by the acid catalyst, whereas the transmittance increased by the base catalyst. In the case of AR coating film prepared using an acid-base two step catalyzed reaction, both the transmittance and abrasion resistance of the film was synergistically enhanced as compared with those of AR coating films prepared without introduction of a catalyst.
Purpose: Solar ultraviolet (UV) radiation causes inflammation and matrix metalloproteinase (MMP) overexpression and extracellular matrix depletion, leading to skin photoaging such as wrinkle formation, dryness, and sagging. Activation of MMP is influenced by various molecules such as reactive oxygen species (ROS), proinflammatory cytokines, and transient receptor potential vanilloid type (TRPV)-1, which are increased in UV-irradiated skin cells. Aralia elata (AE) ethanolic extract was reported to inhibit ROS generation caused by UVB-irradiation in keratinocytes. In this study, we investigated the photoprotective effect of AE ethanolic extract on UVB-irradiated human keratinocytes (HaCaT) and human dermal fibroblasts (HDF). Methods: AE was freeze-dried, extracted in 70% ethanol, and concentrated. Skin cells were treated with AE extract for 24 h and then exposed to UVB ($55mJ/cm^2$). After 48 h of incubation, proinflammatory cytokines, MMP-1, type-1 procollagen, and TRPV-1 levels were measured by ELISA or Western blotting. Results: Treatment with AE extract ($100{\mu}g/mL$) significantly inhibited UVB-induced IL-6, IL-8, and $PGE_2$ production in HaCaT by 25.6%, 5.3%, and 70.2%, respectively, and also inhibited elevation of MMP-1 and TRPV-1 caused by UVB irradiation by 20.0% and 41.9%, respectively (p < 0.05). In HDF, AE extract treatment significantly inhibited both elevation of MMP-1 and reduction of type-1 procollagen caused by UVB irradiation (p < 0.05). In addition, type-1 procollagen was elevated by AE extract treatment in normal HDFs (p < 0.05). Conclusion: AE 70% ethanol extract has photoprotective ability via reduction of proinflammatory mediators, TRPV-1 and MMP-1 production, and elevation of collagen synthesis. Our findings suggest that AE extract might be a good natural material to protect against UVB-induced premature skin aging.
Yeom, Ahram;Kim, Hong Seung;Jang, Nak Won;Yun, Young;Ahn, Hyung Soo
Journal of the Korean Institute of Electrical and Electronic Material Engineers
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v.33
no.3
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pp.214-218
/
2020
In this study, MgxZn1-xO thin films, which can be applied not only to active layers of light-emitting devices (LEDs), such as UV-LEDs, but also to solar cells, high mobility field-effect transistors, and power semiconductor devices, are fabricated using the sol-gel method. ZnO and Mg0.3Zn0.7O solution synthesized by the sol-gel method and the thin film were grown by spin coating on a Si (100) substrate and sapphire substrate. The solutions are synthesized by dissolving precursor materials in 2-methoxyethanol (2-ME) solvent, and then monoethanolamine (MEA) was added to the mixed solution as a sol stabilizer. Zinc acetate dihydrate is used as a ZnO precursor, while Mg nitrate hexahydrate and Mg acetate tetrahydrate are used as an MgO precursor. Then, the optical and structural characteristics of the fabricated thin films are compared. The molar concentration of the Zn precursor in the solvent is fixed at 0.3 M, and the amount of the Mg precursor is 30% of Mg2+/Zn2+. The optical characteristics are measured using an UV-vis spectrophotometer, and the transmittance of each wavelength is measured. Structural characteristics are measured using X-ray diffraction (XRD) and transmission electron microscopy (TEM). Composition analyses are performed using energy dispersive X-ray spectroscopy (EDS). The Mg0.3Zn0.7O thin film was well formed at the ratio of the Mg precursor added regardless of the type of Mg precursor, and the c-axis of the thin film was decreased, while the band gap was increased to 3.56 eV.
Aerosol optical properties as well as vertical location of layer can alter the radiative balance of the Earth by reflecting and absorbing solar radiation. In this study, radiative transfer model (RTM) and satellite-based analysis have been used to quantify the top-of-atmosphere (TOA) radiative effect of aerosol layers in the cloudy atmosphere of the northeast Asia. RTM simulation results show that the atmospheric warming effect of aerosols increases with their height in the presence of underlying cloud layer. This relationship is higher for stronger absorbing aerosols and higher surface albedo condition. Over study region ($20-50^{\circ}N$, $110-140^{\circ}E$) and aerosol event cases, it is possible to qualitatively identify absorbing aerosol effects in the presence of clouds by combining the UV Absorbing Aerosol Index (AAI) derived from Total Ozone Mapping Spectrometer (TOMS), cloud parameters derived from the Moderate Resolution Imaging Spectro-radiometer (MODIS), with TOA Upward Shortwave Flux (USF) from the Clouds and the Earth's Radiant Energy System (CERES). As the regional-mean radiative effect of aerosols, 6 - 26 % lower the USF between aerosols and cloud cover is taken into account. These results demonstrate the importance of estimation for the accurate quantification of aerosol's direct and indirect effect.
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