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Photoprotection and Anti-inflammatory Effects of Chinese Medical Plants  

Jin-Hwa, Kim (R&D Center, Hanbul Cosmetics Co.,)
Sung-Min, Park (R&D Center, Hanbul Cosmetics Co.,)
Gwan-Sub, Sim (R&D Center, Hanbul Cosmetics Co.,)
Bum-Chun , Lee (R&D Center, Hanbul Cosmetics Co.,)
Hyeong-Bae, Pyo (R&D Center, Hanbul Cosmetics Co.,)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.30, no.2, 2004 , pp. 227-233 More about this Journal
Abstract
Chronic exposure to solar radiation, particularly ultraviolet (UV) light, causes a variety of adverse reactions on human skin, such as sunburn, photoaging and photocarcinogenesis. Free radicals and reactive oxygen species (ROS) caused by UV exposure or other environmental facts play critical roles in cellular damage. And, repeated-UV irradiation activated the expression of the matrix metalloproteinase (MMP) and induced skin irritation. Therefore, the development of effective and safe photoprotectants that can reduce and improve the skin damage has been required. The purpose of this study was to investigate the photo-protective effect of several chinese medical plants (Juniperus chinensis) on the UV -induced skin cell damages. We tested free radical and superoxide scavenging effect in vitro. Fluorometric assays of the proteolytic activities of MMP-1 (collagenase) were performed using fluorescent collagen substrates. UVA induced MMP-1 synthesis and activity were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in skin fibroblasts. We also examined anti-inflammatory effects by the determination test of proinflammatory cytokine, interleukin 6 in HaCaT keratinocytes. Expression of prostaglandin E$_2$ (PGE$_2$) after UVB irradiation was measured by competitive enzyme immunoassay(EIA) using PGE$_2$ monoclonal antibody. In the human skin we tested anti-irritation effect on the SLS-induced damage skin after appling the extract containing emulsion. We found that Juniperus chinensis extract had potent radical scavenging effect by 98% at 100$\mu\textrm{g}$/mL. The extract of Juniperus chinensis showed strong inhibitory effect on MMP-1 activities by 97% at 100 $\mu\textrm{g}$/mL and suppressed the UVA induced expression of MMP-1 by 79% at 25$\mu\textrm{g}$/mL. This extract also showed strong inhibition on MMP-2 activity in UVA irradiated fibroblast by zymography. In the test of proinflammatory cytokines of human keratinocytes Juniperus chinensis extract decreased expression of interleukin 6 about 30%. The amount of PGE$_2$ by HaCaT keratinocytes was significantly increased at the doses of above 10 mJ/$\textrm{cm}^2$ of UVB (p < 0.05). At the concentrations of 3.2-25$\mu\textrm{g}$/mL of this extract, the production of PGE$_2$ by HaCaT keratinocytes (24 h after 10mJ/$\textrm{cm}^2$ UVB irradiation) was significantly inhibited in culture supernatants (p < 0.05). In SLS-induced skin irritation model in vivo, we found to reduce skin erythema and improve barrier recovery after appling Juniperus chinensis extract containing emulsion when compared to irritated non-treated and placebo-treated skin. Our results suggest that Juniperus chinensis extract can be effectively used for the prevention of UV and SLS-induced adverse skin reactions and applied as anti-aging and anti-irritation cosmetics.
Keywords
Juniperus chinensis; skin cell protection; anti-inflammation; prostaglandin E$_2$; anti-oxidation;
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1 A. Vanella, A. Russo, R. Acquaviva, and A. Campisi, UVA irradiation induces collagenase in human dermal fibroblasts in vitro and in vivo, Ar. of Dermatol. Res., 283, 509 (1999)
2 Y. Kawaguchi, K. Tanaka, T. Okada, H. Konishi, and M. Takahashi, The effects of ultraviolet A and reactive oxygen species on the mRNA expression of 72-kDa type IV collagenase and its tissue inhibitor in cultured human dermal fibroblasts, Arch. Dermatol. Res., 288, 39 (1996)
3 S. Kondo, The roles of cytokines in photoaging, J. Dermatol. Sci., 23, S30 (2000)
4 M. S. Blois, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199 (1958)
5 T. Agner and J. Serup, Sodium lauryl sulfate for irritant patch testing: a dose response study using bioengineering methods for dertermination of skin irritation, J. Invest. Dermatol., 95, 543 (1990)
6 B. Tebbe, S. Wu, C. C. Geilen, J. Eberle, V. Kodelja, C. E. Orfanos, and C. Christoph, L-Ascorbic acid inhibits UVA-induced lipid peroxidation and secretion of IL-land IL-6 in cultured human keratinocytes in vitro, J. Invest. Dermatol., 108(3), 302 (1997)
7 M. Wlaschek, UVA-induced autocrine stimulation of fibroblast-derived collagenase/MMP-1 by interrelated loops of interleukin-1 and interleukin-6, Photochem. Photobiol., May, 59(5), 550 (1994)
8 A. L. Norins, Free radical formation in the skin following exposure to ultraviolet light, J. Invest. Dermatol., 39, 445 (1962)
9 J. J. Lee, C. W. Lee, Y. H. Cho, S. M. Park, B. C. Lee, and H. B. Pyo, Tinged autunmal leaves of maple and cherry trees as potent antioxidant sources, Cos. & Toil., 115, 39 (2000)
10 M. Demeule, M. Brossard, M. Page, D. Gingras, and R. Beliveau, Matrix metalloproteinase inhibition by green tea catechins, Biochim. Biophys. Acta., 1478(1), 51 (2000)
11 H. C. Korting, T. Herzinger, A. Hartinger, M. Kerscher, T. Angerpointner, and H. I. Maibach, Discrimination of the irritancy potential of surfactants in vitro by two cytotoxicity assays using normal human keratinocytes, HaCaT cells and 3T3 mouse fibroblasts:correlation with in vivo data from a soap chamber assay, J. dermatol. Sci., 7, 119 (1994)
12 A. Pupe, R. Moison, De haes P, van H. G. B. L Rhodes, H. Degreef, and Garmv, Eicosapentaenoic acid, a n-3 polyunsaturated fatty acid differentially modulates TNF-alpha, IL-lalpha, IL-6 and $PGE_2$ expression in UVB-irradiated human keratinocytes, J. Invest. Dermatol., 118, 692 (2002)
13 S. M. Ann, H. Y. Yoon, B. G. Lee, K. C. Park, J. H. Chung, C. H. Moon, and S. H. Lee, Fructose-1,6-diphosphate attenuates prostaglandin $E_2$ production and cyclooxygenase-2 expression in UVB-irradiated HACAT keratinocyte, British J. pharmacology, 137, 497 (2002)
14 K. Furuno, T. Akasako, and N. Sugihara, The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids, Boil. Pharm. Bull., 25, 19 (2002)
15 J. Y. Seo, G. E. Rhie, and J. H. Chung, The effect of ultraviolet irradiation on the expression of type I procollagen and MMP-1 in human dermal fibroblast and human skin in vivo, Kor. J. Invest. Dermiaol., 8, 116 (2001)
16 V. V. PJM, J. P. Nater, and E. Bleumink, Skin irritancy of surfactants as assessed by water vapor loss measurements, J. Invest. Dermatol., 82, 291 (1984)
17 D. Fagot, D. Asselineau, and F. Bernerd, Direct role of human dermal fibroblasts and indirect participation of epidermal keratinocytes in MMP-1 production after UV-B irradiation. Arch. Dermatol. Res., 293, 573 (2002)
18 E. Held, H. Lund, and T. Agner, Effects of different moisturizers on SLS-irritated human skin, Contact Dermatitis, 44, 229 (2000)
19 F. Bonina, In vitro antioxidant and in vivo photoprotective effects of a lyophilized extract of Capparis spinosa L buds, J. Cosmet. Sci. Nov-Dec, 53(6), 321 (2002)
20 Y. Kawaguchi, K. Tanaka, T. Okada, H. Konishi, and M. Takahashi, The effects of ultraviolet A and reactive oxygen species on the mRNA expression of 72-kDa type IV collagenase and its tissue inhibitor in cultured human dermal fibroblasts, Arch. Dermatol. Res., 288, 39 (1996)
21 K. P. Wilhelm and H. I. Maibach, Skin color reflectance measurements for objective quantification of erythema in human beings, J. Am. Acad. Dermatol., 21, 1306 (1989)