• Korean journal of applied entomology
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• v.16 no.2 s.31
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• pp.115-119
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• 1977

Fusarium spp. were isolated from field grown rice, wheat and barley in 1976. The pathogens isolated included Fusarium (Calonectria) nivale, F. (Gibberella) moniliforme and F. (Gibberella) roseum 'Graminearum' and 'Avenaceum'. Among the saprophytes F. (Nectria) episphaeria was isolated. In each of these isolated both the Fusarium and perfect stages were found. F. nivale, and F. episphaeria with there Calonectria, and Nectria stages do not seem to have been recorded previously in Korea. Of the Fusaria isolated, $66.3\%$ from rice were F. moniliforme, and $68.2\%$ from wheat and barley were F. roseum 'Graminearum'. Perithecia also were produced under laboratory conditions. F. moniiforme was recovered wheat heads and also from barley seed.

• Korean Journal Plant Pathology
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• v.11 no.2
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• pp.132-138
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• 1995

우리나라 사과원에서의 토양병 발생현황과 발생실태를 1991년부터 1993년까지 3년간 조사한 결과, 전체 조사주수의 약 8.1%가 각종의 토양 병원균에 감염되어 있는 것으로 나타났다. 토양병 중 자주날개 무늬병의 발생빈도가 5.1%로 가장 높게 조사되었으며, 흰날개무늬병과 역병의 이병주율은 각각 2.5%와 0.2%로 조사되었다. 자주날개무늬병에 감염된 나무에서는 잎의 황화 및 조기낙엽, 신초생장 억제, 과실 생육 불량 등의 지상부 병징이 관찰되었고, 지하부 뿌리 표면에는 적자색 실모양의 균사나 균사속이 관찰되었다. 감염된 뿌리의 표피는 쉽게 벗겨지고 목질부로부터 잘 이탈되는 특징이 있었다. 흰날개무늬병균에 감염된 나무의 뿌리는 흰색의 균사 혹은 균사막으로 싸여 있으며 이 균사막은 시간이 경과하면 회색 내지 흑색으로 착색되었다. 굵은 뿌리의 표피를 제거하면 목질부에 백색 부채모양의 균사막과 실모양의 균사속을 확인할 수 있었다. 이 병원균은 목질부까지 부패시키므로 병의 증세가 심하게 나타났다. 자주날개무늬병은 새로 개원한 10년 이하의 과원에서 발생이 많았다. 자주날개무늬병은 왜화도가 높은 대목에서 발병율이 높았으나 흰날개무늬병은 대목의 종류에 관계없이 발명되었다. 자주날개무늬병은 식양토 과원에서 발생이 많았으며 흰날개무늬병은 사양토 과원에서 발생이 많았다.

• Korean Journal of Medicinal Crop Science
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• v.21 no.2
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• pp.142-147
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• 2013

Gastrodia elata has been cultivated as an important medicinal resources to treat various human diseases. One of the major problems associated with its field production is the degeneration of seed tubers, which is mainly caused by soil-borne pathogens. This study was conducted to produce disease-free seed tubers by the development of in vitro micropropagation method. First, tubers of G. elata were treated with $HgCl_2$ prior to culturing in vitro. Among various culture medium tested, water agar (WA) and WPM medium were the most effective on the induction and growth of vegetative stems. NAA ($0.1mg/{\ell}$) or TDZ ($1.0mg/{\ell}$) in WA medium showed better growth of vegetative stems compared to other plant hormones. Finally the induction and growth of vegetative stems were better in the dark compared to the light condition. In this study, we established an in vitro micropropagation system of G. elata, which might be an efficient way to increase the yield and quality of G. elata tubers in the field production.

• Research in Plant Disease
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• v.22 no.3
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• pp.198-201
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• 2016

Recently, severe dieback of apple tree has occurred in the apple orchards of Chungbuk province. Dieback rate and its casual agents have been investigated on the Chungbuk province apple orchards in 2013-2015. Out of 29,265 apple trees in the 27 orchards throughout Chungbuk province, 4,000 apple trees (13.7%) showed dieback symptoms. The causes of dieback were Phytophthora rot (50.4%), violet root rot by Helicobasidium sp. (27.1%), rodents (10%), white root rot by Rosellinia sp. (6.3%), and freezing injury (6.3%). Compared to previous reports published in 1995 and 2006, Phytophthora rot was the most dominant disease, which is thought to be due to high temperature during growing season and the increase of lowland cultivation. Results of this study will be useful to establish of the management strategy of apple tree dieback that has been increased recently.

• International Journal of Industrial Entomology and Biomaterials
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• v.20 no.1
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• pp.1-5
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• 2010

The studies have been conducted to control the soil borne fungal pathogens viz, Fusarium solani (Mart) Sacc. and Alternaria tenuissima the incitants of root rot and die-back diseases on mulberry stem cuttings planted in the mulberry nurseries and also in established mulberry gardens ten plant extracts with 10% concentration except Lantana camara (undiluted) were tested through poisoned food technique and four biofungicides were also screened by dual culture method under in vitro conditions. Plant extract of Prosopis juliflora showed the maximum inhibition on the mycelial growth (81.2% over A. tenuissima and 80.0% over F. solani) and followed by L. camara (66.7% over A. tenuissima and 68.9% over F. solani). Among the antagonists Pseudomonas fluorescens and Trichoderma viride showed maximum inhibition on the mycelial growth of both pathogenic fungi. The promising plant extracts (P. juliflora and L. camara) and antagonists (P. fluorescens and T. viride) were tested against both the pathogenic fungi under in vivo conditions along with the existing popular chemical Mancozeb. All the tested plant products and bio-fungicides showed inhibitory effect on both fungi. But the maximum survival percentage of mulberry cuttings was recorded in the treatment with T. viride (95% against F. solani and 90% against A. tenuisssima) followed by P. fluorescens (90% against both fungi) and T. harzianum (80% against F. solani and 85% against A. tenuisssima). Incase of the treatments with plant extracts and chemical fungicide the P. juliflora (60% against F. solani and 55% against A. tenuisssima) showed higher survival percentage and followed by L. camara (55% against F. solani and 50% against A. tenuisssima) and Mancozeb (55% against both fungi). In case of control only 10% of survival was recorded in F. solani inoculated cuttings and 15% survival in A. tenuissima inoculated cuttings.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.180-183
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• 2003

P. wasabiae was isolated from discolored seeds of wasabi(Wasabia japonical Mtsum.) and inoculated to fresh seeds, then the effect of fungicides on suppression of diseases were determined. Emergence rate of wasabi seeds where suppressed to 52.5% by the inoculation and it reached up to 92.7% by dipping treatment of inoculated seeds ito benomyl solution. The incidence rate of black leg disease and damping off were 32.0 and 22.0%, respectively, in control treatment that sown in the soil inoculated with P. wasabiae. But dipping treatment of inoculated seeds into benomyl solution resulted in 12.0% and 10.7% in incidence rate of those two diseases, respectively.

• Tuberculosis and Respiratory Diseases
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• v.49 no.6
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• pp.780-784
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• 2000

Bacillus species are aerobic-gram-positive, spore forming rods that are widely distributed in soil, dust, stream, and other environmental sources and are regarded as natural organism. But certain species of the genus Bacillus, most notably B. cereus, which is associated with food-borne illness, occasionally have been implicated in the occurrence of fatal illness and complication in a compromised host. We roport a case of pneumonia and bacteremia caused by B. cereus in an 81 year-old man, who had no obvious immunologic compromise. The condition was treated with combination of roxithromycin and gentamicin.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.396-401
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• 2010

Tolclofos-methyl is one of the most widely used organophosphorous pesticides in control of soil-borne diseases in ginseng field. In Korea, residues of tolclofosmethyl in ginseng and cultivation soil is quite often detecting. The objective of this study was to know the possibility for the accelerated degradation of tolclofos-methyl by various chemical treatment under soil slurry condition. The degradation of tolclofos-methyl was accelerated by zerovalent metals treatment in soil slurry. The degradation rate of tolclofos-methyl was found to be at higher zerovalent zinc than unannealed zerovalent and annealed zerovalent iron. The effect of different sizes of zerovalent iron on tolclofos-methyl degradation was showed that the smaller size of zerovalent iron, the greater the degradation rate. In aqueous solution of pH 4.0 below the degradation rate of tolclofos-methyl was very high. Under this experimental condition, tolclofos-methyl degradation was the greatest at 2% (w/v) of ZVI under 0.1 N of HCl in 24 hours, the degradation rate was 94.4%. By testing various chemicals, it was found that $Fe_2(SO_4)_3$ as iron source showed better for degrading tolclofos-methyl in $H_2O_2$ 500 mM treatment and sodium sulfite also showed the degradable possibility tolclofos-methyl in soil slurry.

• The Korean Journal of Mycology
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• v.20 no.4
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• pp.337-346
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• 1992

Trichoderma spp. are an effective control agent for damping-off or other plant diseases. The interaction between. T. hamatum and Rhizoctonia solani on the rhizosphere or surface soil were examined to assess the possible roles of antibiosis or competition in the mechanisms of biological control agents as a basic research. In a proportional comparison, total bacteria, fungi, actinomycetes and Trichoderma spp were 65%, 8.8%, 25.9% and 0.28% respectively in their distribution in the soil. Among Trichoderma spp isolated, the 5 species of Trichoderma spp were indentified as T. koninggii, T. pseudokoninggii, T. aureoviridi, T. hamatum and T. viride respectively. In a mycoparasitic test, one isolate of T. hamatum strain Tr-5 showed an enzymatic ability to break fungal hyphae into piecies and infected on the R. solani hyphae showing a parasitism. Spore germination of the all isolates of Trichoderma spp showed a 1.7-7.3% of germination in natural soil conditions, but the percentage was high in sterile soil indicating all the natural soil were fungistatic on conidia of Trichoderma spp. In rhizosphere competent assay in pea plant, the antagonistic T. hamatum, T. viride, T. koninggii, T. pseudokoninggii showed a colonizing upper soil depth in rhizosphere around 1-3 cm in root zone, but the colonizing ability was much reduced along the deeper the soil depth. Propagule density was decreased in deeper the soil layer. Disease development rate treated alone with plant pathogens, Fusarium solani, Rhizoctonia solani, Cylindrocarpon destructans increased, but disease incidence rate reduced in treatment with combinations with antagonistic T. hamatum strain Tr-5.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1537-1543
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• 2008

We tested Trichoderma harzianum as a biocontrol agent for Rhizoctonia solani AG2-1, using six natural antifungal materials to improve its efficacy. Among the six materials tested, peony (Paeonia suffruticosa) root bark (PRB) showed the strongest antifungal activity against R. solani AG2-1, and was not antagonistic to T. harzianum. Scanning electron microscopy showed that treatment with PRB extract resulted in shortened and deformed R. so/ani AG2-1 hyphal cells. The control of radish damping-off caused by R. so/ani AG2-1 was greatly increased by combined treatments of T. harzianum and PRB, as compared with either of the two treatments alone, with the control effect increased from 42.3-51.5% to 71.4-87.6%. The antifungal compound in PRB, which was isolated in chloroform and identified as paeonol by mass spectrometry, $^1H$ NMR, and $^{13}C$ NMR analyses, inhibited the growth of R. so/ani AG2-1 but not that of T. harzianum. Thus, PRB powder or extract may be used as a safe additive to T. harzianum to improve the control of the soil borne diseases caused by R. so/ani AG2-1.